FastQCFastQC Report
Sat 18 Jun 2016
SRR3548901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712751
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136840.7989485920603754No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC58760.34307380348924044No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT45800.2674060619436217Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40120.23424303941436903No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT37590.21947148184412094No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC36040.2104217133722298No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT35980.21007139975396305No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33390.19494952856544823No Hit
GGGGAGGGTTGTTGGGGGATAGGAAGAAAATACTATTATACAAGGAATTGGG23260.13580491268141137No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17390.101532563694314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC206.3123245E-446.00000432
ATCGTAC206.3123245E-446.00000428
CGCTAAT206.3123245E-446.00000412
CGAGGAT206.3123245E-446.00000420
TCGCTAA206.3123245E-446.00000411
ACTACGG850.046.0000041
ACGCTAC206.3123245E-446.00000435
CGTACAT206.3123245E-446.00000434
CGTGTAA206.3123245E-446.00000424
TTAGTAC206.3123245E-446.00000413
TATCGAT206.3123245E-446.00000411
GTGCGAT206.3123245E-446.0000049
TCGAACA206.3123245E-446.00000441
TACCGTA206.3123245E-446.00000415
AGTCGAT405.6134013E-946.00000426
CGGACGT206.3123245E-446.00000439
ACAACGT301.8619412E-646.037
AACGTCG301.8619412E-646.028
TCGTTAC253.417862E-546.018
TTTAACG301.8619412E-646.045
Produced by FastQC (version 0.11.5)