##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1712751
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.523130916286135	33.0	31.0	34.0	31.0	34.0
2	32.87213085848439	34.0	31.0	34.0	31.0	34.0
3	32.942051705122346	34.0	31.0	34.0	31.0	34.0
4	36.36639593262535	37.0	37.0	37.0	35.0	37.0
5	36.33962438206137	37.0	37.0	37.0	35.0	37.0
6	36.48303752267551	37.0	37.0	37.0	35.0	37.0
7	36.523445030830516	37.0	37.0	37.0	35.0	37.0
8	36.356830619278576	37.0	37.0	37.0	35.0	37.0
9	38.03412872040361	39.0	39.0	39.0	35.0	39.0
10	37.89797524567202	39.0	38.0	39.0	35.0	39.0
11	37.25014844539574	39.0	35.0	39.0	35.0	39.0
12	37.02359770918248	39.0	35.0	39.0	34.0	39.0
13	36.984068904353286	39.0	35.0	39.0	34.0	39.0
14	38.34974786177325	40.0	35.0	41.0	35.0	41.0
15	38.447227296904224	40.0	35.0	41.0	35.0	41.0
16	38.44466358507454	40.0	35.0	41.0	35.0	41.0
17	38.473723267421825	40.0	36.0	41.0	35.0	41.0
18	38.38409611204431	40.0	36.0	41.0	35.0	41.0
19	38.248165670316354	40.0	35.0	41.0	35.0	41.0
20	38.10777865550801	40.0	35.0	41.0	35.0	41.0
21	38.0377743174577	40.0	35.0	41.0	35.0	41.0
22	38.01111136411539	40.0	35.0	41.0	35.0	41.0
23	37.94969029356865	40.0	35.0	41.0	34.0	41.0
24	37.86774901897591	40.0	35.0	41.0	34.0	41.0
25	37.80381561593016	40.0	35.0	41.0	34.0	41.0
26	37.71074954853332	40.0	35.0	41.0	34.0	41.0
27	37.67452478498042	40.0	35.0	41.0	34.0	41.0
28	37.7039295262417	40.0	35.0	41.0	34.0	41.0
29	37.68591756770248	40.0	35.0	41.0	34.0	41.0
30	37.5699485797994	40.0	35.0	41.0	34.0	41.0
31	37.47256606476949	40.0	35.0	41.0	34.0	41.0
32	37.37651999619326	40.0	35.0	41.0	34.0	41.0
33	37.22866721432362	39.0	35.0	41.0	34.0	41.0
34	37.06186246570576	39.0	35.0	41.0	33.0	41.0
35	36.962015494371336	39.0	35.0	41.0	33.0	41.0
36	36.84171195929823	39.0	35.0	41.0	33.0	41.0
37	36.735831419745196	39.0	35.0	41.0	33.0	41.0
38	36.73723500964238	39.0	35.0	41.0	33.0	41.0
39	36.62770536989907	39.0	35.0	41.0	32.0	41.0
40	36.60662641563193	39.0	35.0	41.0	33.0	41.0
41	36.58836748599183	39.0	35.0	41.0	33.0	41.0
42	36.52932037406488	38.0	35.0	41.0	33.0	41.0
43	36.42930817147384	38.0	35.0	41.0	32.0	41.0
44	36.344828874716754	38.0	35.0	41.0	32.0	41.0
45	36.31168979028475	38.0	35.0	41.0	32.0	41.0
46	36.218513957954194	38.0	35.0	41.0	32.0	41.0
47	36.1516128147057	38.0	35.0	41.0	32.0	41.0
48	36.140852931920634	37.0	35.0	41.0	32.0	41.0
49	36.04271373947527	37.0	35.0	41.0	31.0	41.0
50	35.917365688299114	37.0	35.0	40.0	31.0	41.0
51	35.82201907924736	37.0	35.0	40.0	31.0	41.0
52	35.574218902806074	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	10.0
14	11.0
15	24.0
16	71.0
17	136.0
18	282.0
19	526.0
20	965.0
21	1650.0
22	2652.0
23	4116.0
24	6456.0
25	10362.0
26	14188.0
27	16811.0
28	17063.0
29	18579.0
30	21456.0
31	26100.0
32	32057.0
33	44640.0
34	112374.0
35	293969.0
36	101285.0
37	118128.0
38	184426.0
39	678066.0
40	6339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.55761987586053	21.278808186362173	22.36098533879122	19.802586598986075
2	28.592247209314138	23.19916905609747	25.85688170668124	22.35170202790715
3	28.252559770801476	23.84888404677621	26.7434087033083	21.15514747911401
4	24.444767511447957	26.87209057241829	24.953130957156063	23.73001095897769
5	22.079099647292573	36.50209516736525	23.59829303850939	17.820512146832783
6	84.75073142564213	3.578103296976618	9.037857808870058	2.633307468511185
7	86.67360287630835	2.878059916473556	6.961140294181699	3.487196913036396
8	79.07699367859075	4.770804395968824	11.092038480783255	5.060163444657163
9	45.07000725733046	23.267421826056445	16.526687183367578	15.135883733245523
10	40.09362715304209	21.377946940331665	24.066428803719862	14.461997102906377
11	35.20009621947382	21.490747925413558	26.327177739204355	16.981978115908266
12	21.14925053320652	34.119904177548285	28.280468089056725	16.450377200188466
13	16.331387341183863	37.73870223984689	30.24555233072408	15.684358088245167
14	15.422381887384681	26.189154173607253	42.08311657678203	16.305347362226033
15	17.036218341136568	20.580574759553492	43.14322397126027	19.23998292804967
16	16.918162651780673	22.367874839950467	28.755610126632536	31.958352381636328
17	20.491711871719822	24.95955337349095	32.34136193760798	22.20737281718125
18	24.95873597504833	28.47389959194302	26.90221754358923	19.665146889419418
19	26.548167246727633	25.038724251219236	26.153772498162315	22.259336003890816
20	21.096236405642152	29.393414454290202	29.15140612967092	20.358943010396725
21	19.94277043189582	25.388570784661635	28.01574776485315	26.652911018589393
22	17.199347716042787	31.040647472983522	25.60932674977273	26.150678061200956
23	17.706134750468692	26.960953460251957	36.000095752388994	19.33281603689036
24	19.447368590063586	25.274251773900584	33.27582351433454	22.002556121701286
25	16.11033944805754	33.60377544663527	27.53400815413332	22.751876951173873
26	16.605682904286727	30.852120360752966	30.037057342252314	22.50513939270799
27	17.98007999995329	28.000158808840283	35.046308541054714	18.973452650151714
28	15.851107370540143	27.723819749630856	32.1698834214664	24.2551894583626
29	18.87571515065529	30.003835934120023	29.248034302709502	21.872414612515186
30	26.090190576446897	26.76915675425091	24.66126132753681	22.479391341765382
31	25.763347970604016	28.0847595476517	25.38325769478459	20.76863478695969
32	23.886250832724663	28.3877224418494	23.67343530962761	24.05259141579833
33	18.82480290480052	28.864732818722626	26.285140105012346	26.025324171464504
34	18.095508337172188	26.53082672262343	29.458280859272595	25.915384080931787
35	26.264062902313295	27.637467442728102	24.28414871747265	21.814320937485952
36	19.53815820279772	31.525364749458618	26.572981128021528	22.363495919722133
37	20.3397341469951	31.65159442324074	26.050663523185797	21.958007906578363
38	19.038027127118887	30.503558310577546	25.953685036528952	24.504729525774614
39	23.70799958662993	27.649786804970482	26.65507128590204	21.98714232249755
40	17.2592513447664	25.542533619889873	30.325774149307165	26.872440886036557
41	20.640828701895373	28.957974626784626	24.114363383819367	26.286833287500635
42	20.3602274936637	25.997766166827518	27.165069528495383	26.476936811013392
43	21.436566085788304	28.366061383119906	28.1132517219374	22.084120809154395
44	18.766008602534754	32.83752279227979	24.874368778649085	23.522099826536376
45	22.066313200225835	29.318680882393295	26.61244979567958	22.002556121701286
46	22.477464616864914	27.839131315643662	26.557392172008658	23.126011895482765
47	20.649528156748996	26.535088871645673	29.890684635419863	22.92469833618547
48	20.00979710419086	24.922244973145542	29.534590842451703	25.533367080211892
49	20.011315129870017	28.340663645795566	29.17481875649175	22.473202467842672
50	18.577306333495063	29.994961322457264	27.725221004103922	23.70251133994375
51	20.08966860915568	25.08876071302834	29.224271362270404	25.597299315545573
52	22.01160589017318	23.784835040236437	30.647770750097358	23.555788319493026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4615.0
1	3924.5
2	3234.0
3	10751.0
4	18268.0
5	11514.0
6	4760.0
7	5389.0
8	6018.0
9	7343.0
10	8668.0
11	10131.0
12	11594.0
13	11415.5
14	10667.0
15	10097.0
16	9144.0
17	8191.0
18	7455.0
19	6719.0
20	7118.5
21	7518.0
22	6781.0
23	6044.0
24	6888.0
25	7732.0
26	7739.0
27	7746.0
28	10295.5
29	12845.0
30	16172.0
31	19499.0
32	20163.5
33	20828.0
34	23058.0
35	25288.0
36	29912.5
37	34537.0
38	36521.0
39	45951.0
40	53397.0
41	69637.5
42	85878.0
43	120322.0
44	154766.0
45	164451.5
46	174137.0
47	176760.0
48	179383.0
49	182770.5
50	186158.0
51	168573.5
52	150989.0
53	128872.0
54	106755.0
55	96109.5
56	85464.0
57	75617.0
58	65770.0
59	60039.0
60	54308.0
61	49234.0
62	44160.0
63	40024.0
64	28332.0
65	20776.0
66	16974.5
67	13173.0
68	11091.0
69	9009.0
70	7673.5
71	6338.0
72	5948.0
73	5558.0
74	4371.0
75	3184.0
76	2534.5
77	1885.0
78	1379.0
79	873.0
80	771.5
81	670.0
82	387.5
83	105.0
84	71.0
85	37.0
86	68.0
87	99.0
88	69.5
89	23.5
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1712751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.013633344231643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99985897635587	13.689789194128158
2	11.575748060102024	4.401940585999611
3	4.647182230087929	2.6507945702016182
4	2.4160716015132966	1.8375319825833747
5	1.4998348471094867	1.425865492992075
6	0.9375121716396592	1.0695307612386504
7	0.675347512618042	0.8988566989400812
8	0.48416455848178863	0.7364581914595622
9	0.3555222261981897	0.6083792329191627
>10	2.9808076560722814	12.531142433477532
>50	1.0485248605317055	14.587479532074532
>100	1.3618471096946854	40.65304873488449
>500	0.011410403772271763	1.3820507342679451
>1k	0.005551007240564642	2.3802115170190365
>5k	3.0838929114248014E-4	0.3445451894169896
>10k+	3.0838929114248014E-4	0.8023751483972237
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13684	0.7989485920603754	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5876	0.34307380348924044	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCTT	4580	0.2674060619436217	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4012	0.23424303941436903	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	3759	0.21947148184412094	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3604	0.2104217133722298	No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	3598	0.21007139975396305	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3339	0.19494952856544823	No Hit
GGGGAGGGTTGTTGGGGGATAGGAAGAAAATACTATTATACAAGGAATTGGG	2326	0.13580491268141137	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1739	0.101532563694314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2750545759424458	0.0
2	0.0	0.0	0.0	0.8575093519139676	0.0
3	0.0	0.0	0.0	1.4929198698468138	0.0
4	0.0	0.0	0.0	1.907340880256383	0.0
5	0.0	0.0	0.0	4.092188531783079	0.0
6	0.0	0.0	0.0	4.537028441378811	0.0
7	5.838560304445888E-5	0.0	0.0	5.761257766015025	0.0
8	5.838560304445888E-5	0.0	0.0	7.305848894556185	0.0
9	5.838560304445888E-5	0.0	0.0	8.520152666674841	0.0
10	5.838560304445888E-5	0.0	0.0	9.434704752763245	0.0
11	5.838560304445888E-5	0.0	0.0	10.335185908517934	0.0
12	5.838560304445888E-5	0.0	0.0	11.043987129477665	0.0
13	5.838560304445888E-5	0.0	0.0	11.31145157702433	0.0
14	5.838560304445888E-5	0.0	0.0	11.488593496661219	0.0
15	5.838560304445888E-5	0.0	0.0	12.090081979225234	0.0
16	5.838560304445888E-5	0.0	0.0	12.817508207556148	0.0
17	5.838560304445888E-5	0.0	0.0	13.702342021694923	5.838560304445888E-5
18	5.838560304445888E-5	0.0	0.0	14.095101973374998	5.838560304445888E-5
19	5.838560304445888E-5	0.0	0.0	14.5081217293115	5.838560304445888E-5
20	5.838560304445888E-5	0.0	0.0	15.001596846243267	5.838560304445888E-5
21	1.1677120608891777E-4	0.0	0.0	15.46465306398887	5.838560304445888E-5
22	1.7515680913337666E-4	0.0	0.0	15.970214000750838	5.838560304445888E-5
23	1.7515680913337666E-4	0.0	0.0	16.3452247291054	5.838560304445888E-5
24	2.919280152222944E-4	0.0	0.0	16.635634718648536	5.838560304445888E-5
25	2.919280152222944E-4	0.0	0.0	16.92873044593172	5.838560304445888E-5
26	2.919280152222944E-4	0.0	0.0	17.22754796231326	5.838560304445888E-5
27	3.503136182667533E-4	0.0	0.0	17.525256152236956	5.838560304445888E-5
28	3.503136182667533E-4	0.0	0.0	17.857178305544707	5.838560304445888E-5
29	3.503136182667533E-4	0.0	0.0	18.20655775416275	5.838560304445888E-5
30	4.086992213112122E-4	0.0	0.0	18.54034824676792	5.838560304445888E-5
31	4.086992213112122E-4	0.0	0.0	18.808980406375476	5.838560304445888E-5
32	4.086992213112122E-4	0.0	0.0	19.108148236375282	5.838560304445888E-5
33	4.086992213112122E-4	0.0	0.0	19.42880196829545	5.838560304445888E-5
34	4.086992213112122E-4	0.0	0.0	19.839719842522353	5.838560304445888E-5
35	5.838560304445888E-4	0.0	0.0	20.15214120441325	5.838560304445888E-5
36	5.838560304445888E-4	0.0	0.0	20.469758884975107	5.838560304445888E-5
37	5.838560304445888E-4	0.0	0.0	20.79339028265054	5.838560304445888E-5
38	5.838560304445888E-4	0.0	0.0	21.27483796535515	5.838560304445888E-5
39	6.422416334890478E-4	0.0	0.0	21.609854555624256	5.838560304445888E-5
40	7.006272365335066E-4	0.0	0.0	21.91071556811235	5.838560304445888E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	20	6.3123245E-4	46.000004	32
ATCGTAC	20	6.3123245E-4	46.000004	28
CGCTAAT	20	6.3123245E-4	46.000004	12
CGAGGAT	20	6.3123245E-4	46.000004	20
TCGCTAA	20	6.3123245E-4	46.000004	11
ACTACGG	85	0.0	46.000004	1
ACGCTAC	20	6.3123245E-4	46.000004	35
CGTACAT	20	6.3123245E-4	46.000004	34
CGTGTAA	20	6.3123245E-4	46.000004	24
TTAGTAC	20	6.3123245E-4	46.000004	13
TATCGAT	20	6.3123245E-4	46.000004	11
GTGCGAT	20	6.3123245E-4	46.000004	9
TCGAACA	20	6.3123245E-4	46.000004	41
TACCGTA	20	6.3123245E-4	46.000004	15
AGTCGAT	40	5.6134013E-9	46.000004	26
CGGACGT	20	6.3123245E-4	46.000004	39
ACAACGT	30	1.8619412E-6	46.0	37
AACGTCG	30	1.8619412E-6	46.0	28
TCGTTAC	25	3.417862E-5	46.0	18
TTTAACG	30	1.8619412E-6	46.0	45
>>END_MODULE