FastQCFastQC Report
Sat 18 Jun 2016
SRR3548899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349873
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171801.2727123218258312No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC141021.044690870918968No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG72810.539384075390796No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT28760.21305708018458033Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT25410.18823993071940842Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT20090.14882881574785184No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT18470.13682768675275378Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC18350.13593871423459836No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA17860.13230874311879712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG351.01974365E-746.0000041
ACGATAG351.01974365E-746.0000041
TCATGCG351.01974365E-746.0000041
TAATACG253.4174744E-546.01
ACCGGTA405.6134013E-946.041
CGAACTA206.311848E-446.017
CGTATCC206.311848E-446.012
CGTATAG206.311848E-446.022
CGACGTT253.4174744E-546.028
CGTGAAT206.311848E-446.018
TAGCGAT253.4174744E-546.011
GCTATCG206.311848E-446.040
TATGCGA206.311848E-446.013
CGTTCGA206.311848E-446.029
TACGCTA206.311848E-446.042
ACGATAT253.4174744E-546.026
TATCTCG206.311848E-446.036
TACGAAT900.046.012
CGCTAAT405.6134013E-946.043
CGCTAAA253.4174744E-546.012
Produced by FastQC (version 0.11.5)