##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1349873
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47946732766712	33.0	31.0	34.0	31.0	34.0
2	32.86908472130341	34.0	31.0	34.0	31.0	34.0
3	32.91919980620399	34.0	31.0	34.0	31.0	34.0
4	36.36388460247742	37.0	37.0	37.0	35.0	37.0
5	36.2684037683545	37.0	37.0	37.0	35.0	37.0
6	36.350053671715784	37.0	37.0	37.0	35.0	37.0
7	36.52504272624165	37.0	37.0	37.0	35.0	37.0
8	36.471682891649806	37.0	37.0	37.0	35.0	37.0
9	38.29412692897776	39.0	39.0	39.0	37.0	39.0
10	37.99464097733639	39.0	38.0	39.0	35.0	39.0
11	37.731323613406595	39.0	38.0	39.0	35.0	39.0
12	37.294055811176314	39.0	35.0	39.0	35.0	39.0
13	37.17063457080777	39.0	35.0	39.0	34.0	39.0
14	38.05431844329059	40.0	37.0	41.0	33.0	41.0
15	38.31823215961798	40.0	37.0	41.0	33.0	41.0
16	38.53791949316714	41.0	37.0	41.0	34.0	41.0
17	38.56572507191417	40.0	37.0	41.0	35.0	41.0
18	38.495782936617	40.0	36.0	41.0	35.0	41.0
19	38.43146947898062	40.0	36.0	41.0	35.0	41.0
20	38.28305255383284	40.0	35.0	41.0	34.0	41.0
21	38.232729301200926	40.0	35.0	41.0	34.0	41.0
22	38.18342095886057	40.0	35.0	41.0	34.0	41.0
23	38.159818738503546	40.0	35.0	41.0	34.0	41.0
24	38.09907895039015	40.0	35.0	41.0	34.0	41.0
25	37.9765940944074	40.0	35.0	41.0	34.0	41.0
26	37.86956772970494	40.0	35.0	41.0	34.0	41.0
27	37.76166943112426	40.0	35.0	41.0	34.0	41.0
28	37.739802929608935	40.0	35.0	41.0	34.0	41.0
29	37.686033426848304	40.0	35.0	41.0	34.0	41.0
30	37.63273433871186	40.0	35.0	41.0	34.0	41.0
31	37.498588385722215	40.0	35.0	41.0	33.0	41.0
32	37.3823018906223	40.0	35.0	41.0	33.0	41.0
33	37.25847320451628	40.0	35.0	41.0	33.0	41.0
34	37.056508278926984	40.0	35.0	41.0	33.0	41.0
35	36.951371721636036	40.0	35.0	41.0	33.0	41.0
36	36.840981336762795	39.0	35.0	41.0	32.0	41.0
37	36.68646828257177	39.0	35.0	41.0	32.0	41.0
38	36.689538201001135	39.0	35.0	41.0	32.0	41.0
39	36.639144571378196	39.0	35.0	41.0	32.0	41.0
40	36.55758726932089	39.0	35.0	41.0	31.0	41.0
41	36.42719055792656	39.0	35.0	41.0	31.0	41.0
42	36.46855963486936	39.0	35.0	41.0	31.0	41.0
43	36.449614148886596	39.0	35.0	41.0	31.0	41.0
44	36.42492886367829	39.0	35.0	41.0	31.0	41.0
45	36.37050967016897	39.0	35.0	41.0	31.0	41.0
46	36.30967135426814	38.0	35.0	41.0	31.0	41.0
47	36.20904411007554	38.0	35.0	41.0	31.0	41.0
48	36.10538695121689	38.0	35.0	41.0	31.0	41.0
49	36.069257626458196	38.0	35.0	41.0	31.0	41.0
50	35.96268686017129	38.0	35.0	41.0	31.0	41.0
51	35.8926417522241	38.0	35.0	41.0	31.0	41.0
52	35.662147476095896	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	6.0
14	8.0
15	28.0
16	56.0
17	148.0
18	332.0
19	537.0
20	944.0
21	1518.0
22	2535.0
23	3794.0
24	6000.0
25	9168.0
26	12176.0
27	14074.0
28	14824.0
29	16135.0
30	18253.0
31	21858.0
32	27094.0
33	38154.0
34	100123.0
35	148924.0
36	83500.0
37	98384.0
38	162343.0
39	566062.0
40	2888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.79890330423677	23.229666790875882	26.67280551577815	15.298624389109197
2	35.58490317237251	24.143752782669186	26.852081640272825	13.419262404685478
3	31.461626390038173	24.85159715025043	30.211508786382126	13.475267673329268
4	27.84158213402298	28.153907812068248	29.8216202561278	14.182889797780977
5	25.991630323741568	31.76232134430424	27.477251563665618	14.768796768288572
6	27.71734822461076	39.380741743852944	23.473393422936827	9.428516608599475
7	87.72988273711675	1.6240787096267573	8.285372031294797	2.3606665219616954
8	85.85815110014053	1.9909280354522239	8.795123689413744	3.3557971749934996
9	80.34415089419524	4.406488610410016	11.071485984237036	4.177874511157716
10	44.425734865428076	29.68664459545454	15.008152618801917	10.879467920315467
11	33.88392833992531	23.33286168402509	27.322496264463396	15.460713711586202
12	32.626106307778585	19.071053350944865	29.317572838333678	18.98526750294287
13	28.68440216227749	21.68604009414219	32.762711751401795	16.866845992178522
14	19.14461582682223	24.173162956811492	35.418146744175196	21.264074472191087
15	15.454416822915935	28.86041872087226	37.23579921963029	18.449365236581517
16	26.583537858746713	25.688046208791494	31.483183973603442	16.24523195885835
17	24.631798695136506	24.491711442483847	28.54683366509294	22.329656197286706
18	21.657592973561215	24.514676565869532	33.64523921880058	20.18249124176867
19	22.36558550322882	26.358109244351134	28.615654954206803	22.660650298213238
20	19.03208672223239	34.118468922631976	27.732979324721658	19.116465030413973
21	23.519545912837724	27.844249051577446	32.266739167314256	16.369465868270574
22	21.372455038362865	23.41027637414779	33.27038914031172	21.946879447177622
23	20.2390891587579	25.306528836416465	32.88057469110057	21.57380731372507
24	20.96123116767281	27.408874760810832	31.386137807038146	20.243756264478215
25	22.869114353720683	25.89243580692406	30.095794196935564	21.142655642419694
26	19.67896239127681	29.619527170333804	26.87793592434251	23.82357451404688
27	22.082003269937246	26.683102780780114	30.21802791818193	21.016866031100705
28	18.33846591494163	28.470159785402032	28.181169635958348	25.010204663697994
29	20.84099763459229	27.45954619434569	27.951370240015173	23.748085931046848
30	24.183978789115717	25.61922491967763	26.913346662982367	23.283449628224282
31	22.816294569933614	25.891917239621804	28.65447342083292	22.63731476961166
32	26.735626240394467	25.10925101842914	25.020353766613603	23.134768974562792
33	26.37077710273485	25.546773659447965	27.249081950672398	20.833367287144792
34	21.1182829792136	24.117009526081343	31.513927606522984	23.250779888182073
35	23.583477853101737	27.627858324449782	26.8612676896271	21.927396132821382
36	26.87867673477431	26.14001465323034	25.80116796172677	21.18014065026858
37	21.816719054311033	28.589578427007577	28.415784299708196	21.177918218973193
38	25.33630941577467	24.954866124442816	29.03050879601266	20.67831566376985
39	22.716136999554774	26.824301249080467	27.661935604312404	22.797626147052352
40	27.173519286629187	24.53156704371448	28.884495059905635	19.410418609750696
41	21.039238506140947	24.788924587720473	27.93033122375216	26.241505682386418
42	20.127004540427137	26.29943705815288	30.705703425433356	22.867854975986628
43	20.88144588416836	25.93058754416156	31.106630031121448	22.08133654054863
44	23.430426417892647	25.967998470967267	28.014190964631485	22.587384146508597
45	21.290150999390313	25.61359476039598	26.45182176397335	26.64443247624036
46	23.136028352296844	24.618464107364176	28.097828462381276	24.147679077957704
47	20.49852097197292	25.325049097211366	30.668292498627647	23.508137432188065
48	20.875297157584455	27.781206083831588	28.662177849323605	22.681318909260355
49	24.159235720693726	24.303915998023516	29.697015941499682	21.839832339783076
50	21.612848023480726	25.516622674873858	29.133703689161873	23.736825612483546
51	21.024125973332307	26.082750006852496	27.4696952972613	25.423428722553897
52	20.537932086944473	26.103344536856433	29.827398577495806	23.531324798703285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	584.0
1	2505.5
2	4427.0
3	13247.5
4	22068.0
5	13317.0
6	4566.0
7	4989.0
8	5412.0
9	6621.5
10	7831.0
11	9421.0
12	11011.0
13	11534.0
14	11204.5
15	10352.0
16	9314.0
17	8276.0
18	7436.0
19	6596.0
20	6701.5
21	6807.0
22	7144.5
23	7482.0
24	7716.5
25	7951.0
26	9780.5
27	11610.0
28	12772.5
29	13935.0
30	16870.5
31	19806.0
32	20420.5
33	21035.0
34	24654.0
35	28273.0
36	31475.5
37	34678.0
38	37458.0
39	40557.5
40	40877.0
41	48870.0
42	56863.0
43	61841.5
44	66820.0
45	69405.5
46	71991.0
47	81183.5
48	90376.0
49	106552.5
50	122729.0
51	119980.5
52	117232.0
53	112718.0
54	108204.0
55	96573.0
56	84942.0
57	80277.0
58	75612.0
59	73141.5
60	70671.0
61	57374.0
62	44077.0
63	38772.5
64	28367.0
65	23266.0
66	20719.0
67	18172.0
68	15732.5
69	13293.0
70	11029.5
71	8766.0
72	7542.5
73	6319.0
74	5691.0
75	5063.0
76	4086.0
77	3109.0
78	2339.5
79	1570.0
80	1134.5
81	699.0
82	563.5
83	428.0
84	321.5
85	215.0
86	157.0
87	99.0
88	54.5
89	6.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1349873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.89598838838541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24507171748881	14.373871080092284
2	10.716547244311855	4.264325990728246
3	4.252258891405673	2.5380868058344763
4	2.128727202308887	1.6941252679671108
5	1.3359508776809919	1.3290031574897154
6	0.9017075089239115	1.0764217276362045
7	0.6723305736760599	0.9363676900910769
8	0.517154925391755	0.8231446712472544
9	0.42605074169084617	0.762903054858966
>10	4.150080604071939	19.112184705577427
>50	1.77477425922525	25.712205279733247
>100	0.8684985724285357	22.40920432428596
>500	0.005984486287190541	0.822010663056025
>1k	0.003740303929494088	1.2764011309738312
>5k	3.740303929494088E-4	0.5418304940893461
>10k+	7.480607858988176E-4	2.327913956338817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17180	1.2727123218258312	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	14102	1.044690870918968	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	7281	0.539384075390796	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT	2876	0.21305708018458033	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2541	0.18823993071940842	Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	2009	0.14882881574785184	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1847	0.13682768675275378	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1835	0.13593871423459836	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1786	0.13230874311879712	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2204651845025421	0.0
2	0.0	0.0	0.0	0.6331706760561919	0.0
3	0.0	0.0	0.0	0.9828331998639872	0.0
4	0.0	0.0	0.0	1.3669434087503047	0.0
5	0.0	0.0	0.0	2.5027539627802025	0.0
6	0.0	0.0	0.0	3.6547882652664363	0.0
7	0.0	0.0	0.0	4.637325140957705	0.0
8	0.0	0.0	0.0	6.298962939476529	0.0
9	0.0	0.0	0.0	7.1648221721599	0.0
10	0.0	0.0	0.0	8.034904024304508	0.0
11	0.0	0.0	0.0	9.228497792014508	0.0
12	0.0	0.0	0.0	9.971308411976533	0.0
13	0.0	0.0	0.0	10.306969618623382	0.0
14	7.408104317961763E-5	0.0	0.0	10.507210678337888	0.0
15	7.408104317961763E-5	0.0	0.0	10.719601029133852	0.0
16	7.408104317961763E-5	0.0	0.0	11.0588922068965	0.0
17	7.408104317961763E-5	0.0	0.0	11.427519477758278	0.0
18	7.408104317961763E-5	0.0	0.0	11.831483406216734	0.0
19	7.408104317961763E-5	0.0	0.0	12.101064322347362	0.0
20	1.4816208635923527E-4	0.0	0.0	12.457616383170862	0.0
21	2.222431295388529E-4	0.0	0.0	12.824391627953148	0.0
22	4.444862590777058E-4	0.0	0.0	13.198278652880678	0.0
23	4.444862590777058E-4	0.0	0.0	13.555867848308694	0.0
24	4.444862590777058E-4	0.0	0.0	13.831893815195947	0.0
25	4.444862590777058E-4	0.0	0.0	14.120883964639637	0.0
26	4.444862590777058E-4	0.0	0.0	14.368092405730021	0.0
27	4.444862590777058E-4	0.0	0.0	14.688418836438688	0.0
28	4.444862590777058E-4	0.0	0.0	14.951480620769509	0.0
29	4.444862590777058E-4	0.0	0.0	15.299661523713713	0.0
30	4.444862590777058E-4	0.0	0.0	16.12759126228912	0.0
31	4.444862590777058E-4	0.0	0.0	16.49362569663961	0.0
32	4.444862590777058E-4	0.0	0.0	16.760613776258953	0.0
33	4.444862590777058E-4	0.0	0.0	17.05545632811383	0.0
34	5.185673022573235E-4	0.0	0.0	17.39037672432888	0.0
35	5.185673022573235E-4	0.0	0.0	17.74559532637515	0.0
36	5.185673022573235E-4	0.0	0.0	17.99858208883354	0.0
37	5.926483454369411E-4	0.0	0.0	18.305573931769878	0.0
38	5.926483454369411E-4	0.0	0.0	18.67546058036571	0.0
39	5.926483454369411E-4	0.0	0.0	19.96891559428183	0.0
40	5.926483454369411E-4	0.0	0.0	20.314874065930645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	35	1.01974365E-7	46.000004	1
ACGATAG	35	1.01974365E-7	46.000004	1
TCATGCG	35	1.01974365E-7	46.000004	1
TAATACG	25	3.4174744E-5	46.0	1
ACCGGTA	40	5.6134013E-9	46.0	41
CGAACTA	20	6.311848E-4	46.0	17
CGTATCC	20	6.311848E-4	46.0	12
CGTATAG	20	6.311848E-4	46.0	22
CGACGTT	25	3.4174744E-5	46.0	28
CGTGAAT	20	6.311848E-4	46.0	18
TAGCGAT	25	3.4174744E-5	46.0	11
GCTATCG	20	6.311848E-4	46.0	40
TATGCGA	20	6.311848E-4	46.0	13
CGTTCGA	20	6.311848E-4	46.0	29
TACGCTA	20	6.311848E-4	46.0	42
ACGATAT	25	3.4174744E-5	46.0	26
TATCTCG	20	6.311848E-4	46.0	36
TACGAAT	90	0.0	46.0	12
CGCTAAT	40	5.6134013E-9	46.0	43
CGCTAAA	25	3.4174744E-5	46.0	12
>>END_MODULE