FastQCFastQC Report
Sat 18 Jun 2016
SRR3548894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855283
Sequences flagged as poor quality0
Sequence length46
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298473.48972211537No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71900.8406574198247831No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61440.718358718693111No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36060.42161483392046845No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT22310.2608493329108611No Hit
GGCCCTTTTCCTGCTGAAATTTTTTTCAATACTTGTTTTGCCCAGT16980.19853077870131877No Hit
GTACCAGGGTTGAACAAACTCCTGAGTGTTTATAAATCAAATCAGA15390.1799404407663896No Hit
AAGAAGGGTGGCCGGGGAGATAAAAGTAAAGGCTTGTTTTGTTTTG14120.1650915544913204No Hit
TTGGTAAGGGATGTTTAGAGTTTTATAAGCAAATATAAATACTGTA14050.16427311194072605No Hit
CGGGGGGGAATCTTCCTTAGTCACTCTCTACTTTTTTAAAATCATC13330.15585484570604116No Hit
AGATATGGGATTTTCTTATGGTTTGTAATATTTCTGTAAATTGTGA12830.15000882748751No Hit
AAGACAGGGGAGGAAGGCATGATAAGTAATTTAAACCTGTATCATG12200.1426428445321607No Hit
GACATTGGTGTTGATTATTAACAGGTAACTAAATCAGATATTTATT12130.14182440198156634No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.13890139287230074No Hit
AAGTTAGGGTTCTCTTTTAGGGAGTTTGACACCTCTGTAGCCTGCA11850.1385506317791889No Hit
AGAGAAGGGCAGAACATTTGTTTGTATGGTTTTAAACCAAATGTTA11560.1351599412124408No Hit
GTACAGGAAATTAAAGTTTTGTTTGAGACTTCATAAGCTTGGTGCA11510.1345753393905877No Hit
GCTCACGGGGCTTTTAGTTTACCTATTTTTACTAATGTATGATGTT11490.13434149866184641No Hit
AACACAGGGATAGTCAAGATGTCAAGTATAAAAGGGTATATATGTC11480.1342245782974758No Hit
TTGATAGGGTCTAATGACATAATTGCTTTGCTTTGCTTTATTTCTT11440.1337568968399933No Hit
AGGATAGGGTTTTTTTTTTGTTTGTTTCTGTTTTGTTTTGTTTGTT11380.13305537465376957No Hit
TTGTCAGGGATATGCTTACAGTGCACCTAGAGCTTTTTATAACAGC11320.13235385246754583No Hit
ACAATCGGGATTCTAAACCATTTTTAAGTGGTCTGGACAGTGAGTT10640.12440326769034343No Hit
ACAGATGGGGTAAAAGGTTTTGATCTTGATGACCTTCTGAAAAACT10580.12370174550411969No Hit
GGCTAGCGAGGAAGTGTATTTAAAAAGAAACCTACATTTCCAGCAA10490.12264946222478408No Hit
CCCGCAGGGAGAGGAGCTTTAAATTATTAATGATCCTTTGGCTTTA10470.12241562149604282No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGT10250.1198433734798891No Hit
TAACGAGGGACTGTAATTTTTTAAATTTTTTTTTGTTCTTTTTTGT10230.11960953275114787No Hit
GATCATGGGAATTTTGAGTGATTACTAATATTTATTTATTTATTTT10050.11750496619247663No Hit
CAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTT10030.11727112546373539No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT9690.1132958330751342No Hit
CAGCTTGGGGTATATTAGATTATTTATCAGAAAAGTATCAAACATT9640.11271123125328109No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTAT9510.11119126651646298No Hit
CGTTGCGGGCAATTAGGTTCTTGATTTTAAGCATATTTTTTCATAT9470.11072358505898047No Hit
ACTGTAGGGGGTATAAAATTGTGAAAGACAGTAATATGATCATGAA9400.10990514250838611No Hit
AGGCTGCCTGCTTAATGATTAATATTTTTCATACATTTTTTTTTAA9380.10967130177964486No Hit
ATAGAAGGGATTATTTTGTAGGAAGAAGACTTGTATACAATGTAGA9340.10920362032216238No Hit
TTATTAGGGGGGGGATTATGAGCTAATTTTTTTTATAGGACACAGA9180.10733289449223239No Hit
ACTTAAGGGCATTATTTATGTTTTTTTTCCCTTTGTTCATATTCTT9110.10651445194163803No Hit
TGAGTTGGGCTCTACTATAGCTGTACAAAAAAAAGTTTGCTTTTTT9070.10604677048415553No Hit
AGATGAGTCGGGAATTATATAATATTAAAAGAAATCACACAGAGGA9030.10557908902667304No Hit
AGATAAGGGTATGCACTAACCTTTTTTGGTGGCTAATTAGGGTTTA8990.10511140756919056No Hit
TGAATTGGGGGGCTTTTCGTTTGTTGTTTGTTTGTTTGTTTTCACA8890.10394220392548432No Hit
CAACAAGGGGGGGGAGTACTACTTTCTTAAGTTTCAAAGGAAACTG8880.10382528356111369No Hit
CGCATAGGGGTTTTTGTTTTTTTTAATGTATACCATAGTACCAGTA8850.10347452246800182No Hit
ACCAGAGGGAAATATGTTCTTTCAAGCATTCTCTTTATATATATTT8840.10335760210363121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG304.866053E-640.01
ATCCTAT200.001255588540.027
CGGAATG200.001255588540.01
GTATTCG451.2205419E-940.036
CCTAGTT750.040.031
CGAAACG257.79298E-540.01
ACGCATT600.040.015
ACGTTGA257.79298E-540.031
ACGTTAT257.79298E-540.011
TCACACG401.9274012E-840.01
ACCGCAA200.001255588540.014
CTATACG900.040.01
ATTACCG304.866053E-640.01
GACCGAC200.001255588540.010
ATTCGAC257.79298E-540.024
CGCGACG401.9274012E-840.01
ACTTACG257.79298E-540.01
ATCCGCA200.001255588540.033
ACGATTG200.001255588540.01
GTTACGA555.456968E-1240.013
Produced by FastQC (version 0.11.5)