##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855283
Sequences flagged as poor quality	0
Sequence length	46
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74115702054174	33.0	31.0	34.0	30.0	34.0
2	31.964813985546304	33.0	31.0	34.0	30.0	34.0
3	32.12457981744054	34.0	31.0	34.0	30.0	34.0
4	35.85918929757753	37.0	35.0	37.0	35.0	37.0
5	35.55722491853574	37.0	35.0	37.0	33.0	37.0
6	35.628075151733405	37.0	35.0	37.0	33.0	37.0
7	35.597892159671126	37.0	35.0	37.0	33.0	37.0
8	35.90366229657318	37.0	35.0	37.0	35.0	37.0
9	37.35285630604139	39.0	37.0	39.0	34.0	39.0
10	36.78438949447142	39.0	37.0	39.0	32.0	39.0
11	36.949011029097974	39.0	37.0	39.0	33.0	39.0
12	36.71684109236358	39.0	35.0	39.0	33.0	39.0
13	36.55247327492771	39.0	35.0	39.0	32.0	39.0
14	37.77675576388166	40.0	37.0	41.0	33.0	41.0
15	37.63823085458264	39.0	36.0	41.0	33.0	41.0
16	37.59466632681814	39.0	36.0	41.0	33.0	41.0
17	37.4797277626236	39.0	36.0	40.0	33.0	41.0
18	37.29369927848443	39.0	36.0	40.0	33.0	41.0
19	37.29999894771672	39.0	35.0	41.0	32.0	41.0
20	37.156197422373644	39.0	35.0	40.0	32.0	41.0
21	37.20368930517735	39.0	35.0	40.0	32.0	41.0
22	37.24614308948033	39.0	35.0	41.0	33.0	41.0
23	37.11097145623145	39.0	35.0	41.0	32.0	41.0
24	36.82277561929794	39.0	35.0	40.0	32.0	41.0
25	36.93764753888479	39.0	35.0	40.0	32.0	41.0
26	36.88642823486495	39.0	35.0	40.0	32.0	41.0
27	36.73151342888845	39.0	35.0	40.0	31.0	41.0
28	36.712330304706164	39.0	35.0	40.0	31.0	41.0
29	36.41486502128535	38.0	35.0	40.0	31.0	41.0
30	36.40336941106043	38.0	35.0	40.0	31.0	41.0
31	36.292829390973516	38.0	35.0	40.0	31.0	41.0
32	36.19551657170784	38.0	35.0	40.0	30.0	41.0
33	36.161793231012425	39.0	35.0	40.0	30.0	41.0
34	35.987236973025304	38.0	35.0	40.0	30.0	41.0
35	35.77004804257772	38.0	35.0	40.0	29.0	41.0
36	35.754744336085245	38.0	35.0	40.0	29.0	41.0
37	35.61889339551938	38.0	35.0	40.0	28.0	41.0
38	35.55822224924382	38.0	35.0	40.0	28.0	41.0
39	35.475032240790476	38.0	35.0	40.0	27.0	41.0
40	35.32335963651797	38.0	35.0	40.0	26.0	41.0
41	35.28723708994567	38.0	35.0	40.0	26.0	41.0
42	35.190961354311966	38.0	35.0	40.0	26.0	41.0
43	35.12996400021981	38.0	34.0	40.0	26.0	41.0
44	35.0725257020191	38.0	34.0	40.0	25.0	41.0
45	34.94833172178098	38.0	34.0	40.0	25.0	41.0
46	34.114150520938686	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	18.0
11	31.0
12	36.0
13	39.0
14	68.0
15	98.0
16	153.0
17	257.0
18	425.0
19	768.0
20	1371.0
21	2246.0
22	3308.0
23	4835.0
24	6654.0
25	8379.0
26	9423.0
27	10391.0
28	11400.0
29	12966.0
30	15834.0
31	19905.0
32	26394.0
33	37363.0
34	61663.0
35	86629.0
36	80956.0
37	103676.0
38	174803.0
39	175179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.97892393511855	31.93539448346337	20.20851577781857	25.87716580359951
2	24.482656617751083	34.3030318619685	19.613274202807727	21.601037317472695
3	20.088204722881198	36.06233258465327	20.354198551824368	23.49526414064117
4	19.57433972147231	36.47248922286541	19.802334431995025	24.150836623667253
5	16.90960769710143	39.085659366548846	18.77600747354969	25.228725462800032
6	17.305967732317843	44.35678015346967	20.454983905911845	17.882268208300644
7	78.57048485705901	12.28400424187082	5.748038953188594	3.3974719478815785
8	80.1912349479646	11.496896348927782	5.100066293846598	3.2118024092610282
9	75.29800077868963	14.294917588681175	7.228367686485059	3.178713946144142
10	34.60597252605278	36.70726531452163	14.741553380576955	13.945208778848638
11	25.693717751902	33.28009559408991	25.368679138951666	15.657507515056421
12	25.216331904176748	31.246382776227282	28.947728412700823	14.589556906895146
13	21.88842757309569	35.22740426268264	27.331772056734437	15.55239610748723
14	17.257445781104032	37.02961475909143	29.663748724106526	16.04919073569801
15	16.486823659537254	34.73131115665809	31.9117765698605	16.870088613944155
16	19.41182041499714	32.619378615031515	31.288357187036336	16.680443782935008
17	17.363492551588187	32.18572098358087	30.643190616439238	19.807595848391703
18	17.925411822753404	32.80154054272095	31.298295418007843	17.974752216517807
19	18.723860991040393	35.28095378956439	29.36361414876713	16.631571070628084
20	20.667194367244527	33.62278918206021	29.48252215933206	16.227494291363207
21	20.24207192239294	33.793609834405686	29.469543998886916	16.494774244314456
22	18.512936653715787	32.74460032527246	29.54121618224611	19.20124683876565
23	16.93626554017793	34.65998973439201	28.786027548776254	19.61771717665381
24	16.64747224018249	34.20762484464206	32.593188453412495	16.55171446176295
25	18.163344764247626	34.424044439092086	29.20518705504494	18.207423741615347
26	18.19666706809325	35.80732926996094	29.27533927366731	16.7206643882785
27	17.164727932158126	35.62154281097602	29.731445615077114	17.48228364178874
28	17.49701560769944	36.109568411859	29.73460246491512	16.65881351552644
29	16.48998050937526	36.18474820614931	30.036841606813187	17.288429677662247
30	20.573190394290545	34.3444216709557	28.65566134250301	16.426726592250752
31	20.399680573564538	36.74234142383281	25.72201248007969	17.135965522522955
32	20.362616818059053	34.199791180229234	27.75794678486536	17.679645216846353
33	18.64809659492823	33.939994130597704	28.2497138374082	19.16219543706586
34	18.461374773028343	34.058785220798256	28.717161454161953	18.76267855201144
35	19.070997552856774	35.2821229932081	27.45804605025471	18.18883340368042
36	21.21391399104156	33.02474151830447	28.80602093108363	16.95532355957034
37	18.2493981524244	34.67378633738774	28.791288965172928	18.285526545014925
38	19.30694284815669	34.20797560573518	28.4467246513727	18.038356894735426
39	18.722457946667944	33.57321494756707	29.37214933536619	18.332177770398804
40	20.53308670931142	31.914699578969767	28.41036241805344	19.141851293665372
41	18.857851728609127	32.242310439936254	29.29580033743217	19.604037494022446
42	19.57071519017682	31.821864809659495	29.22833728719032	19.379082712973368
43	18.009945246193364	31.156003334568794	30.66295015801787	20.17110126121997
44	19.414509583377665	31.59971611735531	30.22414803053492	18.761626268732105
45	19.933869841911974	32.98580703696905	29.479131468765306	17.601191652353666
46	19.309982777630328	32.71583791563728	30.01018376373668	17.96399554299571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36058.0
1	27657.0
2	19256.0
3	12352.5
4	5449.0
5	5214.5
6	4980.0
7	4980.0
8	5165.0
9	5350.0
10	5625.5
11	5901.0
12	5613.5
13	5326.0
14	5663.5
15	6001.0
16	5695.5
17	5390.0
18	6260.5
19	7131.0
20	7131.0
21	7672.5
22	8214.0
23	9225.5
24	10237.0
25	15320.0
26	20403.0
27	17724.5
28	15046.0
29	19837.0
30	24628.0
31	24628.0
32	31749.0
33	38870.0
34	39342.5
35	39815.0
36	44787.0
37	49759.0
38	47419.5
39	45080.0
40	48739.0
41	52398.0
42	53199.0
43	54000.0
44	54000.0
45	56287.5
46	58575.0
47	56849.5
48	55124.0
49	55708.0
50	56292.0
51	53127.5
52	49963.0
53	43529.5
54	37096.0
55	34770.5
56	32445.0
57	32445.0
58	29342.0
59	26239.0
60	24020.5
61	21802.0
62	18744.5
63	15687.0
64	14097.5
65	12508.0
66	11164.5
67	9821.0
68	8082.5
69	6344.0
70	6344.0
71	5436.5
72	4529.0
73	4203.5
74	3878.0
75	3011.5
76	2145.0
77	1940.0
78	1735.0
79	1357.0
80	979.0
81	979.0
82	693.0
83	407.0
84	311.0
85	215.0
86	169.0
87	123.0
88	76.0
89	29.0
90	20.5
91	12.0
92	12.5
93	13.0
94	13.0
95	11.5
96	10.0
97	10.5
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	855283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.51574254257013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1494928518428	14.284801469345759
2	9.402370186384987	3.481837313220831
3	3.2735547432960375	1.8183689047763605
4	1.6506727543249815	1.2225372696446588
5	0.9506486361515514	0.8800982697713776
6	0.7097681901857074	0.7885131044630705
7	0.47221655931747963	0.6120408165662525
8	0.38100047358292327	0.5643605341966961
9	0.3031457719803614	0.5051672160171338
>10	3.664651828661901	16.14957742112385
>50	1.209863370067385	15.897905067310356
>100	0.7500517795535862	26.437084644455965
>500	0.06350988819251364	8.093004294143478
>1k	0.017147669811978686	4.1869085160701305
>5k	0.001270197763850273	1.5679887137559085
>10k+	6.350988819251365E-4	3.5098064451381883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29847	3.48972211537	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7190	0.8406574198247831	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6144	0.718358718693111	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3606	0.42161483392046845	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	2231	0.2608493329108611	No Hit
GGCCCTTTTCCTGCTGAAATTTTTTTCAATACTTGTTTTGCCCAGT	1698	0.19853077870131877	No Hit
GTACCAGGGTTGAACAAACTCCTGAGTGTTTATAAATCAAATCAGA	1539	0.1799404407663896	No Hit
AAGAAGGGTGGCCGGGGAGATAAAAGTAAAGGCTTGTTTTGTTTTG	1412	0.1650915544913204	No Hit
TTGGTAAGGGATGTTTAGAGTTTTATAAGCAAATATAAATACTGTA	1405	0.16427311194072605	No Hit
CGGGGGGGAATCTTCCTTAGTCACTCTCTACTTTTTTAAAATCATC	1333	0.15585484570604116	No Hit
AGATATGGGATTTTCTTATGGTTTGTAATATTTCTGTAAATTGTGA	1283	0.15000882748751	No Hit
AAGACAGGGGAGGAAGGCATGATAAGTAATTTAAACCTGTATCATG	1220	0.1426428445321607	No Hit
GACATTGGTGTTGATTATTAACAGGTAACTAAATCAGATATTTATT	1213	0.14182440198156634	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1188	0.13890139287230074	No Hit
AAGTTAGGGTTCTCTTTTAGGGAGTTTGACACCTCTGTAGCCTGCA	1185	0.1385506317791889	No Hit
AGAGAAGGGCAGAACATTTGTTTGTATGGTTTTAAACCAAATGTTA	1156	0.1351599412124408	No Hit
GTACAGGAAATTAAAGTTTTGTTTGAGACTTCATAAGCTTGGTGCA	1151	0.1345753393905877	No Hit
GCTCACGGGGCTTTTAGTTTACCTATTTTTACTAATGTATGATGTT	1149	0.13434149866184641	No Hit
AACACAGGGATAGTCAAGATGTCAAGTATAAAAGGGTATATATGTC	1148	0.1342245782974758	No Hit
TTGATAGGGTCTAATGACATAATTGCTTTGCTTTGCTTTATTTCTT	1144	0.1337568968399933	No Hit
AGGATAGGGTTTTTTTTTTGTTTGTTTCTGTTTTGTTTTGTTTGTT	1138	0.13305537465376957	No Hit
TTGTCAGGGATATGCTTACAGTGCACCTAGAGCTTTTTATAACAGC	1132	0.13235385246754583	No Hit
ACAATCGGGATTCTAAACCATTTTTAAGTGGTCTGGACAGTGAGTT	1064	0.12440326769034343	No Hit
ACAGATGGGGTAAAAGGTTTTGATCTTGATGACCTTCTGAAAAACT	1058	0.12370174550411969	No Hit
GGCTAGCGAGGAAGTGTATTTAAAAAGAAACCTACATTTCCAGCAA	1049	0.12264946222478408	No Hit
CCCGCAGGGAGAGGAGCTTTAAATTATTAATGATCCTTTGGCTTTA	1047	0.12241562149604282	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGT	1025	0.1198433734798891	No Hit
TAACGAGGGACTGTAATTTTTTAAATTTTTTTTTGTTCTTTTTTGT	1023	0.11960953275114787	No Hit
GATCATGGGAATTTTGAGTGATTACTAATATTTATTTATTTATTTT	1005	0.11750496619247663	No Hit
CAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTT	1003	0.11727112546373539	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT	969	0.1132958330751342	No Hit
CAGCTTGGGGTATATTAGATTATTTATCAGAAAAGTATCAAACATT	964	0.11271123125328109	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTAT	951	0.11119126651646298	No Hit
CGTTGCGGGCAATTAGGTTCTTGATTTTAAGCATATTTTTTCATAT	947	0.11072358505898047	No Hit
ACTGTAGGGGGTATAAAATTGTGAAAGACAGTAATATGATCATGAA	940	0.10990514250838611	No Hit
AGGCTGCCTGCTTAATGATTAATATTTTTCATACATTTTTTTTTAA	938	0.10967130177964486	No Hit
ATAGAAGGGATTATTTTGTAGGAAGAAGACTTGTATACAATGTAGA	934	0.10920362032216238	No Hit
TTATTAGGGGGGGGATTATGAGCTAATTTTTTTTATAGGACACAGA	918	0.10733289449223239	No Hit
ACTTAAGGGCATTATTTATGTTTTTTTTCCCTTTGTTCATATTCTT	911	0.10651445194163803	No Hit
TGAGTTGGGCTCTACTATAGCTGTACAAAAAAAAGTTTGCTTTTTT	907	0.10604677048415553	No Hit
AGATGAGTCGGGAATTATATAATATTAAAAGAAATCACACAGAGGA	903	0.10557908902667304	No Hit
AGATAAGGGTATGCACTAACCTTTTTTGGTGGCTAATTAGGGTTTA	899	0.10511140756919056	No Hit
TGAATTGGGGGGCTTTTCGTTTGTTGTTTGTTTGTTTGTTTTCACA	889	0.10394220392548432	No Hit
CAACAAGGGGGGGGAGTACTACTTTCTTAAGTTTCAAAGGAAACTG	888	0.10382528356111369	No Hit
CGCATAGGGGTTTTTGTTTTTTTTAATGTATACCATAGTACCAGTA	885	0.10347452246800182	No Hit
ACCAGAGGGAAATATGTTCTTTCAAGCATTCTCTTTATATATATTT	884	0.10335760210363121	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1692036437062353E-4	0.0	0.0	0.3072667175659986	0.0
2	1.1692036437062353E-4	0.0	0.0	0.41506729351571353	0.0
3	1.1692036437062353E-4	0.0	0.0	0.555605571489203	0.0
4	1.1692036437062353E-4	0.0	0.0	1.2166733116407085	0.0
5	1.1692036437062353E-4	0.0	0.0	1.3319567909101433	0.0
6	1.1692036437062353E-4	0.0	0.0	1.8744672815898364	0.0
7	1.1692036437062353E-4	0.0	0.0	2.2675535466038728	0.0
8	1.1692036437062353E-4	0.0	0.0	2.6138716658696595	0.0
9	1.1692036437062353E-4	0.0	0.0	3.1197860825013475	0.0
10	1.1692036437062353E-4	0.0	0.0	3.839314004838165	0.0
11	1.1692036437062353E-4	0.0	0.0	4.645947598631096	0.0
12	1.1692036437062353E-4	0.0	0.0	4.980690601824191	0.0
13	1.1692036437062353E-4	0.0	0.0	5.1579418742100565	0.0
14	1.1692036437062353E-4	0.0	0.0	5.325254915624419	0.0
15	1.1692036437062353E-4	0.0	0.0	5.445916731654902	0.0
16	1.1692036437062353E-4	0.0	0.0	5.674496043999471	0.0
17	1.1692036437062353E-4	0.0	0.0	5.944815926424353	0.0
18	1.1692036437062353E-4	0.0	0.0	6.289731001317692	0.0
19	1.1692036437062353E-4	0.0	0.0	6.449911900505446	0.0
20	1.1692036437062353E-4	0.0	0.0	6.6038960203815575	0.0
21	1.1692036437062353E-4	0.0	0.0	6.815171118799275	0.0
22	1.1692036437062353E-4	0.0	0.0	7.022120163735278	0.0
23	1.1692036437062353E-4	0.0	0.0	7.248361068792435	0.0
24	1.1692036437062353E-4	0.0	0.0	7.407723525429595	0.0
25	1.1692036437062353E-4	0.0	0.0	7.568372106074832	0.0
26	1.1692036437062353E-4	0.0	0.0	7.702245923279195	0.0
27	1.1692036437062353E-4	0.0	0.0	7.8389258292284545	0.0
28	1.1692036437062353E-4	0.0	0.0	7.980984071938762	0.0
29	1.1692036437062353E-4	0.0	0.0	8.129823695782566	0.0
30	1.1692036437062353E-4	0.0	0.0	8.311634862378884	0.0
31	1.1692036437062353E-4	0.0	0.0	8.465502061890625	0.0
32	1.1692036437062353E-4	0.0	0.0	8.631061297839429	0.0
33	1.1692036437062353E-4	0.0	0.0	8.79626977269512	0.0
34	1.1692036437062353E-4	0.0	0.0	8.941134104150322	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	4.866053E-6	40.0	1
ATCCTAT	20	0.0012555885	40.0	27
CGGAATG	20	0.0012555885	40.0	1
GTATTCG	45	1.2205419E-9	40.0	36
CCTAGTT	75	0.0	40.0	31
CGAAACG	25	7.79298E-5	40.0	1
ACGCATT	60	0.0	40.0	15
ACGTTGA	25	7.79298E-5	40.0	31
ACGTTAT	25	7.79298E-5	40.0	11
TCACACG	40	1.9274012E-8	40.0	1
ACCGCAA	20	0.0012555885	40.0	14
CTATACG	90	0.0	40.0	1
ATTACCG	30	4.866053E-6	40.0	1
GACCGAC	20	0.0012555885	40.0	10
ATTCGAC	25	7.79298E-5	40.0	24
CGCGACG	40	1.9274012E-8	40.0	1
ACTTACG	25	7.79298E-5	40.0	1
ATCCGCA	20	0.0012555885	40.0	33
ACGATTG	20	0.0012555885	40.0	1
GTTACGA	55	5.456968E-12	40.0	13
>>END_MODULE