SRR3548893_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725505
Sequences flagged as poor quality	0
Sequence length	46
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23819	3.283092466626694	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5195	0.7160529562167043	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4107	0.5660884487357082	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2644	0.3644358067828616	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	2350	0.32391230935693066	No Hit
GATAGAGGGATAGAACAACTTTTTTTTCACTTTATTACTGAACTTT	1305	0.17987470796204025	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGT	1269	0.17491264705274256	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	1264	0.17422347192645124	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGT	1256	0.17312079172438508	No Hit
AGCTTTGGGCTCTAAAAACTAAAGGCTAAACATGCCTTTTAGATTA	1212	0.16705605061302128	No Hit
TCCAAAGGGACTTTCAATTTTTTTTACATTGAATTAAATGCAGTAT	1142	0.1574075988449425	No Hit
TCGAATGGGTAGATCTATAGGCATTGATTGTTCTTTGATTTAAAGA	1039	0.14321059124334085	No Hit
AGGCGAGGGAGAAAGAAAGAGAGGAGAGAAAGAAAGAGAGAAGAAG	1010	0.13921337551085106	No Hit
CAGACGACTTGATGAAGATCTTAATTTTGTTTTTGGTTTAAAGTAG	974	0.1342513146015534	No Hit
GTAGATGGGAAGGTTTTTCTTTTCCCCACAGATTTCATACCTAAAA	962	0.13259729429845418	No Hit
AAACCGGGGGAAATTATACTCCTGAGATGTCAAGAGGGAAGCGTAA	920	0.1268082232376069	No Hit
AAGTTTGGGGAATTTCTTGTTGTATTTCTCTCTTGTTTTAGCAGTA	920	0.1268082232376069	No Hit
GGGGATGGGTTTACATTATTTTTTCCTGCCGACCTGGTTTGATTTT	853	0.11757327654530292	No Hit
CTAGCCGGGAGCTTAAAGAAACTTGACCAAAAGACAGTATTAAAAA	838	0.1155057511664289	No Hit
AAAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	824	0.11357606081281314	No Hit
GAACATGGATTTTTAGATTTATTTGAAAGGGAAAGATGATGAATAT	816	0.11247338061074699	No Hit
AGAAGCGGGAAGTTCCTATGGTTTACATGAACTCCTAAGACTATGA	807	0.11123286538342259	No Hit
ATCTTTGGGTGGTTGGATTCTAAGAGAATCAAACCGAGAGACATGA	806	0.11109503035816432	No Hit
AAGACAGGTCTCTCATCAAGATCCAGATTTCTGTTCTTTTTTGATA	803	0.1106815252823895	No Hit
AACAACGGGTGGTATATCAGAAACAAAATGTTTTTATTTGTACTAT	781	0.10764915472670761	No Hit
CCGGTAGGGAGTCCCAACAATTATTTATTTTTAGAAAGTAAATTTA	766	0.10558162934783358	No Hit
ATAACCGGGAACTTTTTTATTGTTTGATGGGAAAAATTAAGATTTA	754	0.10392760904473436	No Hit
ACCGATGGGAATATTTTTATTTGTTGTTTCTTGATATTTTCTTATT	751	0.10351410396895955	No Hit
AACAGCGGGAACTTGTTTATTTTTAATCTCGAGTATTTAGTTGCCA	738	0.10172224864060206	No Hit
TCCGGAGGGATCATCTTTTCTTTCAAAAATACTTATCAAACTGTCA	735	0.10130874356482726	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTC	30	4.865027E-6	40.000004	12
GGCCGAT	30	4.865027E-6	40.000004	8
CCTACCG	30	4.865027E-6	40.000004	1
CGACAAG	30	4.865027E-6	40.000004	1
GCCGATG	30	4.865027E-6	40.000004	9
CTAACCG	30	4.865027E-6	40.000004	1
CGACTCC	30	4.865027E-6	40.000004	13
CTTACGG	120	0.0	40.000004	2
CTTGTCG	20	0.0012554628	40.0	29
TTGATAC	40	1.9266736E-8	40.0	14
GTTTGCG	20	0.0012554628	40.0	33
TTTAGCG	25	7.791808E-5	40.0	1
CTCGTAT	20	0.0012554628	40.0	40
GCGCGTA	20	0.0012554628	40.0	7
TTAGCCC	20	0.0012554628	40.0	40
ACGCAGT	20	0.0012554628	40.0	21
ACGCAAC	25	7.791808E-5	40.0	30
TGGACGC	20	0.0012554628	40.0	22
AGCCCAC	20	0.0012554628	40.0	2
CGTTGTG	40	1.9266736E-8	40.0	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content