FastQCFastQC Report
Sat 18 Jun 2016
SRR3548892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490971
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185123.770487462599624No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43550.887017766833479No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38000.7739764670418415No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT27610.5623550067111907TruSeq Adapter, Index 13 (100% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20400.4155031559908834No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT17760.36173215933323966No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC15010.3057207044815274No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT14190.2890191070348351TruSeq Adapter, Index 13 (100% over 22bp)
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT9030.18392124993125866TruSeq Adapter, Index 13 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT7440.15153644512608686No Hit
GGCACGTGGGGAGCAACATGGAATAAGAAAAGGTCAAGGGAGGCAGAGGCA5260.10713463728000228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGG453.8380676E-1045.0000042
TTCCGAG351.2100281E-745.0000041
TGCGGCC302.1629512E-645.00000439
TCGATCT302.1629512E-645.00000419
AGAACCG351.2100281E-745.0000041
GTAATGC351.2100281E-745.00000431
GTAATCG351.2100281E-745.00000410
CCGTAGT351.2100281E-745.00000427
CTAGTTA302.1629512E-645.00000426
CGTAGTG351.2100281E-745.00000428
TGTTCGG351.2100281E-745.0000042
ACTACCG302.1629512E-645.0000041
GTCGTCT302.1629512E-645.00000418
CGTACTC302.1629512E-645.00000445
TACTCGC302.1629512E-645.00000445
CCTATTC302.1629512E-645.00000433
CGCAAAG700.045.0000041
CAATCTA351.2100281E-745.00000418
CGATCTT302.1629512E-645.00000420
TCGAAGG351.2100281E-745.0000042
Produced by FastQC (version 0.11.5)