##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490971
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011206364530697	31.0	31.0	33.0	28.0	34.0
2	31.166626949453228	31.0	31.0	34.0	28.0	34.0
3	31.414028527143152	31.0	31.0	34.0	30.0	34.0
4	35.365828124268035	37.0	35.0	37.0	33.0	37.0
5	34.94728201869357	35.0	35.0	37.0	32.0	37.0
6	34.90585390990507	35.0	35.0	37.0	32.0	37.0
7	34.09621138519383	35.0	35.0	37.0	30.0	37.0
8	34.47095856985443	36.0	35.0	37.0	32.0	37.0
9	35.58931179234619	39.0	35.0	39.0	30.0	39.0
10	35.26012941701241	37.0	35.0	39.0	29.0	39.0
11	35.66819628857916	37.0	35.0	39.0	30.0	39.0
12	35.46106592853753	37.0	35.0	39.0	30.0	39.0
13	35.1575836454699	37.0	34.0	39.0	29.0	39.0
14	36.14436494212489	38.0	35.0	40.0	30.0	41.0
15	36.316114393721826	38.0	35.0	40.0	31.0	41.0
16	36.32958158424836	38.0	35.0	40.0	31.0	41.0
17	36.290736112723565	38.0	35.0	40.0	31.0	41.0
18	36.01885854765353	37.0	35.0	40.0	30.0	41.0
19	35.875495701375435	37.0	34.0	40.0	30.0	41.0
20	35.75717710414668	37.0	34.0	40.0	30.0	41.0
21	35.74763071545977	37.0	34.0	40.0	30.0	41.0
22	35.69160296636665	37.0	34.0	40.0	30.0	41.0
23	35.52645879288186	37.0	34.0	40.0	29.0	41.0
24	35.151218707418565	37.0	33.0	40.0	27.0	41.0
25	35.23119695460628	37.0	34.0	40.0	27.0	41.0
26	35.09755769689045	36.0	33.0	40.0	27.0	41.0
27	34.9145081888747	36.0	33.0	40.0	27.0	41.0
28	34.82739306394879	36.0	33.0	40.0	26.0	41.0
29	34.63541023807924	36.0	33.0	40.0	25.0	41.0
30	34.57853518843272	36.0	33.0	40.0	25.0	41.0
31	34.338097362165996	36.0	33.0	39.0	24.0	41.0
32	34.22115359155632	36.0	33.0	39.0	24.0	41.0
33	34.08910098559793	36.0	33.0	40.0	24.0	41.0
34	34.05307645461748	36.0	33.0	40.0	23.0	41.0
35	33.89691040815038	36.0	33.0	40.0	23.0	41.0
36	33.69311222047738	35.0	33.0	39.0	22.0	41.0
37	33.51083057858814	35.0	33.0	39.0	21.0	41.0
38	33.350034930780026	35.0	32.0	39.0	21.0	40.0
39	33.227844414435886	35.0	32.0	39.0	21.0	40.0
40	33.103714883363786	35.0	32.0	39.0	20.0	40.0
41	33.15193565404066	35.0	32.0	39.0	20.0	40.0
42	33.03297343427616	35.0	32.0	39.0	18.0	40.0
43	32.947974523953555	35.0	32.0	39.0	18.0	40.0
44	32.856129995457984	35.0	32.0	39.0	18.0	40.0
45	32.607253788920325	35.0	31.0	39.0	18.0	40.0
46	32.4668402003377	35.0	31.0	39.0	17.0	40.0
47	32.37007073737553	35.0	31.0	39.0	17.0	40.0
48	32.22007002450246	35.0	31.0	39.0	15.0	40.0
49	32.075059423061646	35.0	31.0	39.0	15.0	40.0
50	31.889999613011767	35.0	31.0	39.0	13.0	40.0
51	29.971354723598747	34.0	26.0	37.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	2.0
11	8.0
12	10.0
13	20.0
14	31.0
15	68.0
16	169.0
17	368.0
18	874.0
19	1568.0
20	2483.0
21	3675.0
22	4974.0
23	6194.0
24	7024.0
25	7706.0
26	8403.0
27	9688.0
28	11282.0
29	13951.0
30	16952.0
31	21182.0
32	27192.0
33	36648.0
34	54728.0
35	56428.0
36	48209.0
37	58833.0
38	62076.0
39	30219.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.079741980687253	36.925195174460406	18.97342205547782	25.021640789374526
2	19.600342993781712	38.99008291732098	19.692609135773804	21.716964953123505
3	18.366868918938188	40.363280112267326	18.87361982683295	22.396231141961543
4	16.77207004079671	40.46165659478869	18.67075652126093	24.095516843153668
5	16.045143195830303	42.691930887975055	15.619048782922004	25.643877133272635
6	17.157225172158842	44.03192856604565	17.71265512627019	21.098191135525315
7	72.30671465320762	18.611893574162224	5.090321016923607	3.9910707557065486
8	75.73298626599127	16.20686354183852	3.74971230480008	4.31043788737013
9	69.98315582794096	20.322585244342335	6.195885296687584	3.498373631029124
10	35.29088276089627	44.142933085660864	9.540074668361267	11.026109485081603
11	28.346888105407448	33.010503675369826	25.207598819482207	13.435009399740514
12	27.886168429499907	32.68950711956511	25.282144973939396	14.142179476995587
13	20.00444018078461	42.90620016253506	20.615881589747666	16.47347806693267
14	13.965386957681819	45.72164140040858	25.038138708803576	15.274832933106028
15	12.389326457163458	39.35629599304236	31.195528860156706	17.058848689637472
16	15.251613639094774	36.485046978334765	30.829723140470616	17.433616242099838
17	13.967627415875885	35.3752054602003	25.445087388053473	25.212079735870347
18	17.566006953567523	37.037625440199115	25.09353912960236	20.302828476631003
19	19.25510875387752	38.45237295074455	23.680013687162784	18.61250460821515
20	22.119432715985262	38.18107383124462	22.14529982422587	17.55419362854425
21	17.872949726154904	38.95280984009239	23.092606284281555	20.08163414947115
22	18.253012907075977	37.192420733607484	22.311501086622222	22.243065272694317
23	16.954769222622108	39.21188827853376	20.592458617718766	23.240883881125363
24	15.144682679832414	38.52956691943108	27.037849486018523	19.287900914717977
25	15.477085204625121	37.78471640891213	25.31941805116799	21.418780335294752
26	16.438445447898147	43.69198995460017	20.161475932387045	19.70808866511464
27	14.000826932751629	40.672463343048776	24.299602216831545	21.027107507368054
28	14.477433494035289	41.49450782225427	25.849795609109293	18.178263074601148
29	14.852201046497655	38.4535950188504	24.957685891834753	21.736518042817192
30	16.783272331767048	40.32926588332101	22.10436054267971	20.78310124223223
31	19.96859284967951	39.71293620193453	20.129294805599514	20.18917614278644
32	21.10063527173703	38.03809186285952	19.7284564668789	21.132816398524557
33	19.27649494572999	39.24019952298608	19.962482509150234	21.52082302213369
34	16.235582142326123	38.200626920938305	22.537176330170215	23.026614606565357
35	16.40443121895183	37.326033513181024	21.98704200451758	24.282493263349565
36	19.857384652046658	35.59782553348364	23.922797884192754	20.62199193027694
37	16.494456902749857	38.80738373549558	22.18623910577203	22.51192025598253
38	18.141804709443125	37.74703597564826	21.83530188137385	22.27585743353477
39	18.41982520352526	36.10702057759012	24.114255220776787	21.35889899810783
40	20.11320424220575	34.42117762556241	22.05873666672777	23.406881465504075
41	16.760053037755796	31.06497125084781	27.543582003825072	24.631393707571323
42	20.80998674056105	32.358937696931186	22.825177047116835	24.005898515390932
43	18.834106291410286	32.32594185807308	24.066187208613137	24.773764641903494
44	18.542846726181384	34.70795627440317	23.475520957449625	23.273676041965818
45	19.434549087420642	38.944662719386685	21.34851141920806	20.272276773984615
46	18.934112198072796	35.45932448148669	25.060135934708978	20.54642738573154
47	18.49681549419416	35.16969434039892	23.26023329280141	23.073256872605512
48	19.677740640485894	31.161921987245684	28.003283289644397	21.157054082624025
49	20.171659833269175	29.762450328023448	26.603811630422165	23.462078208285213
50	18.33163262188602	33.262860739229	23.59528363182347	24.810223007061516
51	17.507958718539385	33.92338855044392	25.9363587666074	22.632293964409303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21303.0
1	16743.0
2	12183.0
3	8060.5
4	3938.0
5	3462.5
6	2987.0
7	3119.5
8	3252.0
9	3492.0
10	3732.0
11	3977.0
12	4222.0
13	4349.0
14	4476.0
15	4550.0
16	4624.0
17	4326.5
18	4029.0
19	3871.5
20	3714.0
21	3489.5
22	3265.0
23	3197.5
24	3130.0
25	3377.0
26	4316.5
27	5009.0
28	5524.0
29	6039.0
30	6961.0
31	7883.0
32	9456.0
33	11029.0
34	10855.0
35	10681.0
36	12105.0
37	13529.0
38	14174.0
39	14819.0
40	18248.5
41	21678.0
42	27167.0
43	32656.0
44	38471.5
45	44287.0
46	44803.0
47	45319.0
48	45163.5
49	45008.0
50	41310.0
51	37612.0
52	33302.5
53	28993.0
54	25751.5
55	22510.0
56	19150.5
57	15791.0
58	14350.5
59	12910.0
60	11659.5
61	10409.0
62	9577.0
63	8745.0
64	7406.5
65	6068.0
66	4865.0
67	3662.0
68	3131.5
69	2601.0
70	1957.0
71	1313.0
72	1379.0
73	1445.0
74	1138.0
75	762.5
76	694.0
77	539.5
78	385.0
79	348.0
80	311.0
81	221.0
82	131.0
83	103.0
84	75.0
85	57.5
86	40.0
87	29.5
88	19.0
89	13.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	490971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.52963580647352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81626469177208	27.09739922354683
2	8.533648038418365	5.7226022165759
3	2.995363045876994	3.013002961092746
4	1.5254564005726798	2.045919901994238
5	0.9147042121507369	1.5334849552030752
6	0.5895546475299231	1.1860531571815314
7	0.44017446379074987	1.0331222623549527
8	0.337342860441709	0.9048786602019623
9	0.2611119786827689	0.7879490594946829
>10	2.6494103987714652	19.876190528047395
>50	0.6489082709783622	15.356315101446642
>100	0.28127582848491745	13.514152936184006
>500	0.001850498871611299	0.4494238671961043
>1k	0.004317830700426364	3.65081919178354
>5k	0.0	0.0
>10k+	6.168329572037663E-4	3.8286859776964026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18512	3.770487462599624	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4355	0.887017766833479	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3800	0.7739764670418415	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	2761	0.5623550067111907	TruSeq Adapter, Index 13 (100% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2040	0.4155031559908834	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT	1776	0.36173215933323966	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1501	0.3057207044815274	No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1419	0.2890191070348351	TruSeq Adapter, Index 13 (100% over 22bp)
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	903	0.18392124993125866	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	744	0.15153644512608686	No Hit
GGCACGTGGGGAGCAACATGGAATAAGAAAAGGTCAAGGGAGGCAGAGGCA	526	0.10713463728000228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.073560352851798E-4	0.0	0.0	0.8257106835230594	0.0
2	4.073560352851798E-4	0.0	0.0	1.0212415804599457	0.0
3	4.073560352851798E-4	0.0	0.0	1.2933554120304458	0.0
4	4.073560352851798E-4	0.0	0.0	2.823384680561581	0.0
5	4.073560352851798E-4	0.0	0.0	3.0411164814215095	0.0
6	4.073560352851798E-4	0.0	0.0	4.16419707070275	0.0
7	4.073560352851798E-4	0.0	0.0	5.07647091172391	0.0
8	4.073560352851798E-4	0.0	0.0	5.601145485171222	0.0
9	4.073560352851798E-4	0.0	0.0	6.8879832006371045	0.0
10	4.073560352851798E-4	0.0	0.0	8.151805300109375	0.0
11	4.073560352851798E-4	0.0	0.0	9.729495224768876	0.0
12	4.073560352851798E-4	0.0	0.0	10.340936633731932	0.0
13	4.073560352851798E-4	0.0	0.0	10.603884954508514	0.0
14	4.073560352851798E-4	0.0	0.0	10.871314191673235	0.0
15	4.073560352851798E-4	0.0	0.0	11.088434958480237	0.0
16	4.073560352851798E-4	0.0	0.0	11.538359699452718	0.0
17	4.073560352851798E-4	0.0	0.0	12.141450309692425	0.0
18	4.073560352851798E-4	0.0	0.0	12.879579445629172	0.0
19	4.073560352851798E-4	0.0	0.0	13.213204038527733	0.0
20	4.073560352851798E-4	0.0	0.0	13.537052086579452	0.0
21	4.073560352851798E-4	0.0	0.0	13.889415057101132	0.0
22	4.073560352851798E-4	0.0	0.0	14.300029940668594	0.0
23	4.073560352851798E-4	0.0	0.0	14.724902285471035	0.0
24	4.073560352851798E-4	0.0	0.0	15.015754494664654	0.0
25	4.073560352851798E-4	0.0	0.0	15.261186505923975	0.0
26	4.073560352851798E-4	0.0	0.0	15.51558034995957	0.0
27	4.073560352851798E-4	0.0	0.0	15.745736509895696	0.0
28	4.073560352851798E-4	0.0	0.0	15.997278861684295	0.0
29	4.073560352851798E-4	0.0	0.0	16.25187638373753	0.0
30	4.073560352851798E-4	0.0	0.0	16.603831998223928	0.0
31	4.073560352851798E-4	0.0	0.0	16.881241458253136	0.0
32	4.073560352851798E-4	0.0	0.0	17.15457735792949	0.0
33	4.073560352851798E-4	0.0	0.0	17.421599239058928	0.0
34	4.073560352851798E-4	0.0	0.0	17.69004686631186	0.0
35	4.073560352851798E-4	0.0	0.0	17.99149033242289	0.0
36	4.073560352851798E-4	0.0	0.0	18.29171173042807	0.0
37	4.073560352851798E-4	0.0	0.0	18.55771522146929	0.0
38	4.073560352851798E-4	0.0	0.0	18.812720099557815	0.0
39	4.073560352851798E-4	0.0	0.0	19.09949874839858	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAGG	45	3.8380676E-10	45.000004	2
TTCCGAG	35	1.2100281E-7	45.000004	1
TGCGGCC	30	2.1629512E-6	45.000004	39
TCGATCT	30	2.1629512E-6	45.000004	19
AGAACCG	35	1.2100281E-7	45.000004	1
GTAATGC	35	1.2100281E-7	45.000004	31
GTAATCG	35	1.2100281E-7	45.000004	10
CCGTAGT	35	1.2100281E-7	45.000004	27
CTAGTTA	30	2.1629512E-6	45.000004	26
CGTAGTG	35	1.2100281E-7	45.000004	28
TGTTCGG	35	1.2100281E-7	45.000004	2
ACTACCG	30	2.1629512E-6	45.000004	1
GTCGTCT	30	2.1629512E-6	45.000004	18
CGTACTC	30	2.1629512E-6	45.000004	45
TACTCGC	30	2.1629512E-6	45.000004	45
CCTATTC	30	2.1629512E-6	45.000004	33
CGCAAAG	70	0.0	45.000004	1
CAATCTA	35	1.2100281E-7	45.000004	18
CGATCTT	30	2.1629512E-6	45.000004	20
TCGAAGG	35	1.2100281E-7	45.000004	2
>>END_MODULE