FastQCFastQC Report
Sat 18 Jun 2016
SRR3548891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544493
Sequences flagged as poor quality0
Sequence length46
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235844.3313688146587745No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57141.049416613253063No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT52360.961628524149989No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45970.8442716435289342No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25560.4694275224842193No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT17070.31350265292666757No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT14840.27254712181791135No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT14730.2705268938259996No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGT13310.24444758702132077No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC11410.20955273988829975No Hit
GGCACGTGGGGAGCAACATGGAATAAGAAAAGGTCAAGGGAGGCAG6310.11588762389966446No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC6160.11313276754705755No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.10982693992392922No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.10082774250541329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC850.040.021
TCTGCGT304.862788E-640.037
GAGTGTA200.00125518740.026
CGGGTAC353.052428E-740.06
GATACTC200.00125518740.09
CTGTCGG200.00125518740.02
TCGTTCA257.789243E-540.016
GCCTTAC451.2187229E-940.040
GTCGCAA200.00125518740.020
ACACGAC850.040.026
CTGTATG200.00125518740.021
GTATTCG200.00125518740.09
ATTAGCG257.789243E-540.01
GGTAAGT200.00125518740.08
ATCTACG304.862788E-640.01
TTTACGG507.8216544E-1140.02
GAATGCG304.862788E-640.01
CGCGGCA200.00125518740.04
CATGCGA555.456968E-1240.010
TGAAGCG200.00125518740.024