##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544493
Sequences flagged as poor quality	0
Sequence length	46
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.894147399507432	31.0	31.0	33.0	28.0	34.0
2	31.20562064158768	31.0	31.0	34.0	28.0	34.0
3	31.39815020578777	31.0	31.0	34.0	28.0	34.0
4	35.40993364469332	37.0	35.0	37.0	33.0	37.0
5	34.946184799437276	35.0	35.0	37.0	32.0	37.0
6	35.07502024819419	36.0	35.0	37.0	32.0	37.0
7	34.91390706583923	36.0	35.0	37.0	32.0	37.0
8	35.3289629067775	37.0	35.0	37.0	33.0	37.0
9	36.70644985334982	39.0	37.0	39.0	32.0	39.0
10	35.97101156488697	37.0	35.0	39.0	31.0	39.0
11	36.22400838945588	37.0	35.0	39.0	32.0	39.0
12	35.90749192367946	37.0	35.0	39.0	31.0	39.0
13	35.46945507104775	37.0	35.0	39.0	30.0	39.0
14	36.57567314914976	38.0	35.0	40.0	31.0	41.0
15	36.576494096342834	38.0	35.0	40.0	31.0	41.0
16	36.51182659832174	38.0	35.0	40.0	31.0	41.0
17	36.481811520074636	38.0	35.0	40.0	31.0	41.0
18	36.230766970374276	37.0	35.0	40.0	31.0	41.0
19	36.33769763798616	38.0	35.0	40.0	31.0	41.0
20	36.05918717045031	37.0	34.0	40.0	30.0	41.0
21	36.137322242893845	37.0	34.0	40.0	30.0	41.0
22	36.20844345106365	38.0	34.0	40.0	31.0	41.0
23	35.98181611150189	37.0	34.0	40.0	31.0	41.0
24	35.547782983435965	37.0	34.0	40.0	29.0	41.0
25	35.770733508052444	37.0	34.0	40.0	30.0	41.0
26	35.760215466498195	37.0	34.0	40.0	30.0	41.0
27	35.63437913802381	37.0	34.0	40.0	30.0	41.0
28	35.617286172641336	37.0	34.0	40.0	30.0	41.0
29	35.36157856942146	37.0	34.0	40.0	29.0	41.0
30	35.2813057284483	37.0	34.0	40.0	28.0	41.0
31	35.155434505126784	37.0	34.0	40.0	27.0	41.0
32	35.05370500630862	37.0	34.0	40.0	27.0	41.0
33	34.97565074298476	37.0	34.0	40.0	27.0	41.0
34	34.93098350208359	37.0	34.0	40.0	26.0	41.0
35	34.71920851140419	37.0	34.0	40.0	25.0	41.0
36	34.65098724868823	37.0	33.0	40.0	25.0	41.0
37	34.47142938476711	36.0	33.0	40.0	24.0	41.0
38	34.243184026240925	36.0	33.0	40.0	23.0	41.0
39	34.233154512546534	36.0	33.0	40.0	23.0	41.0
40	34.108458694602135	36.0	33.0	40.0	23.0	41.0
41	34.223345387360354	36.0	33.0	40.0	23.0	41.0
42	34.07827097869027	36.0	33.0	40.0	23.0	41.0
43	34.01186057488342	36.0	33.0	40.0	22.0	41.0
44	33.89290587757786	36.0	33.0	40.0	22.0	41.0
45	33.66010949635716	35.0	33.0	40.0	22.0	41.0
46	32.725575902720514	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	5.0
12	12.0
13	10.0
14	21.0
15	29.0
16	71.0
17	135.0
18	322.0
19	657.0
20	1264.0
21	2008.0
22	2917.0
23	4021.0
24	5389.0
25	6535.0
26	7648.0
27	8466.0
28	9448.0
29	11703.0
30	14730.0
31	19060.0
32	25388.0
33	35638.0
34	59124.0
35	68508.0
36	55719.0
37	69894.0
38	85821.0
39	49943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.68215018374892	36.20285292923876	18.938351824541364	26.17664506247096
2	20.144060621532326	37.93051517650365	19.050749963727725	22.874674238236302
3	18.449089336318373	39.86038388004988	18.83844236748682	22.852084416144926
4	16.330053829893128	40.300793582286644	18.889499038555133	24.479653549265095
5	16.05218065246018	42.02404805938736	15.938313256552425	25.98545803160004
6	17.52878365745749	43.32948265634269	17.969560673874597	21.172173012325228
7	73.32895005078119	16.67753304450195	5.699797793543718	4.2937191111731465
8	75.99987511317869	15.190094271184387	4.229255472522144	4.580775143114788
9	70.60862123112693	18.48765732525487	7.149770520465829	3.753950923152364
10	34.76334865645656	43.67475798586942	9.850448031471478	11.71144532620254
11	27.523769818895744	32.387744195058524	26.25120984108152	13.837276144964214
12	27.269404749005037	32.44155572247944	25.916035651514346	14.373003877001173
13	19.831292596966353	42.12689602988468	21.28548943696246	16.75632193618651
14	13.782729989182595	44.843735364825626	25.699136628019094	15.674398017972683
15	12.133122005241573	38.916570093646754	31.23125549823414	17.719052402877537
16	14.793027642228642	36.4667681678185	30.777071514234343	17.963132675718512
17	13.697880413522304	35.2627122846391	25.82953316204249	25.209874139796103
18	17.190487297357357	36.390550475396374	25.324476164064553	21.094486063181712
19	18.82981048424865	38.523727577765	24.39517128778515	18.251290650201195
20	21.653170931490394	37.921148664904784	22.776785009173672	17.64889539443115
21	17.366614446834028	39.01978537832442	23.477253151096527	20.136347023745024
22	17.78204678480715	36.937848604114286	22.820862710815383	22.45924190026318
23	17.03988848341485	39.03723280189093	20.675931554675632	23.246947160018586
24	14.652713625335863	38.43924531628506	27.04938355497683	19.858657503402245
25	15.160341822576232	37.826014292194756	25.435956017800045	21.577687867428967
26	16.28505784280055	43.214513317893896	20.69980697639823	19.800621862907327
27	13.543975771956665	40.26442947843223	24.412067740081138	21.779527009529968
28	14.366759535935264	41.33808882758823	25.88334468946341	18.411806947013094
29	14.730951545749898	38.44438771480993	24.824194250431137	22.000466489009042
30	16.511874349165186	40.25138982502989	21.847663789984445	21.389072035820476
31	19.39069923763942	39.78269693090636	20.306229832155786	20.52037399929843
32	20.667116014347293	38.00085584204021	19.59051815174851	21.741509991863992
33	18.782794269164157	38.74962579867877	20.314310744123432	22.15326918803364
34	15.686335728833981	38.1466795716382	22.685323778267122	23.481660921260698
35	16.00920489335951	37.18413276203735	21.8963329188805	24.910329425722644
36	19.257915161443766	35.360234199521386	24.371479523152733	21.010371115882116
37	16.089279384675283	38.82694543364194	21.809463115228294	23.27431206645448
38	17.828144714440775	37.65870268304643	22.394227290341657	22.11892531217114
39	18.19766277987045	35.695775703268914	24.37625460749725	21.73030690936339
40	19.74846324929797	34.147179118923475	22.375310610053756	23.729047021724796
41	16.32289120337635	30.86412497497672	27.79943911124661	25.013544710400314
42	20.683828809553106	32.13962346623372	23.073574866894525	24.102972857318644
43	18.31777451684411	32.0143693307352	24.515283024758812	25.15257312766188
44	18.420622487341433	34.29024799216886	23.525738622902406	23.763390897587296
45	19.287851267142095	38.25411896938987	22.011485914419467	20.446543849048567
46	18.31299943249959	35.110276899794854	25.988580202132994	20.58814346557256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27157.0
1	21352.5
2	15548.0
3	10275.0
4	5002.0
5	4481.5
6	3961.0
7	3961.0
8	3990.5
9	4020.0
10	4032.5
11	4045.0
12	4263.5
13	4482.0
14	4412.0
15	4342.0
16	4305.5
17	4269.0
18	4165.0
19	4061.0
20	4061.0
21	3883.5
22	3706.0
23	3635.5
24	3565.0
25	3826.0
26	4087.0
27	5211.0
28	6335.0
29	7442.5
30	8550.0
31	8550.0
32	9359.0
33	10168.0
34	11037.0
35	11906.0
36	13330.5
37	14755.0
38	16456.0
39	18157.0
40	23091.5
41	28026.0
42	34662.0
43	41298.0
44	41298.0
45	46770.5
46	52243.0
47	58276.0
48	64309.0
49	56957.0
50	49605.0
51	45473.5
52	41342.0
53	35370.0
54	29398.0
55	25568.0
56	21738.0
57	21738.0
58	18582.5
59	15427.0
60	14082.0
61	12737.0
62	11711.5
63	10686.0
64	9289.0
65	7892.0
66	6183.5
67	4475.0
68	3521.5
69	2568.0
70	2568.0
71	2024.0
72	1480.0
73	1420.0
74	1360.0
75	1169.5
76	979.0
77	685.0
78	391.0
79	327.0
80	263.0
81	263.0
82	193.0
83	123.0
84	74.5
85	26.0
86	16.0
87	6.0
88	5.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	544493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.005587129897375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95235823998789	20.511380281711716
2	10.11904824253198	5.465416779706646
3	3.7115526449386302	3.0069797502027376
4	1.9308169597806728	2.085713825569621
5	1.2442419403585103	1.6800742065512166
6	0.8498786997115229	1.3770883964942053
7	0.653758178593077	1.2358586417714847
8	0.47485488429968475	1.025898796160998
9	0.3742881858316524	0.9097084992751119
>10	3.4464071880268508	20.55875005168607
>50	0.7968909744733488	15.293760616071117
>100	0.43566104688431584	17.309717775437473
>500	0.003414271527306551	0.536260832367121
>1k	0.004779980138229171	2.635017549413271
>5k	0.0013657086109226202	2.019276518026126
>10k+	6.828543054613101E-4	4.349097479555083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23584	4.3313688146587745	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5714	1.049416613253063	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	5236	0.961628524149989	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4597	0.8442716435289342	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2556	0.4694275224842193	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT	1707	0.31350265292666757	No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT	1484	0.27254712181791135	No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT	1473	0.2705268938259996	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGT	1331	0.24444758702132077	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1141	0.20955273988829975	No Hit
GGCACGTGGGGAGCAACATGGAATAAGAAAAGGTCAAGGGAGGCAG	631	0.11588762389966446	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	616	0.11313276754705755	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	598	0.10982693992392922	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	549	0.10082774250541329	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.102126198132942	0.0
2	0.0	0.0	0.0	1.3708165210572036	0.0
3	0.0	0.0	0.0	1.7419875002984428	0.0
4	0.0	0.0	0.0	3.532827786583115	0.0
5	0.0	0.0	0.0	3.802803709138593	0.0
6	0.0	0.0	0.0	5.18298674179466	0.0
7	0.0	0.0	0.0	6.221016615456948	0.0
8	0.0	0.0	0.0	6.850225806392369	0.0
9	0.0	0.0	0.0	8.245836034623034	0.0
10	0.0	0.0	0.0	9.78763730663204	0.0
11	0.0	0.0	0.0	11.596108673573397	0.0
12	0.0	0.0	0.0	12.353326856359953	0.0
13	0.0	0.0	0.0	12.68078744813983	0.0
14	0.0	0.0	0.0	12.987862102910414	0.0
15	0.0	0.0	0.0	13.246451285875116	0.0
16	0.0	0.0	0.0	13.781628046641554	0.0
17	0.0	0.0	0.0	14.500829211762134	0.0
18	0.0	0.0	0.0	15.360895365046016	0.0
19	0.0	0.0	0.0	15.753003252567067	0.0
20	0.0	0.0	0.0	16.123623260537784	0.0
21	0.0	0.0	0.0	16.56623684785663	0.0
22	0.0	0.0	0.0	17.033644142348937	0.0
23	0.0	0.0	0.0	17.520886402580015	0.0
24	0.0	0.0	0.0	17.86322321866397	0.0
25	0.0	0.0	0.0	18.14642245171196	0.0
26	0.0	0.0	0.0	18.439355510539162	0.0
27	0.0	0.0	0.0	18.71796331633281	0.0
28	0.0	0.0	0.0	19.005753976635145	0.0
29	0.0	0.0	0.0	19.29593217910974	0.0
30	0.0	0.0	0.0	19.693182465155658	0.0
31	0.0	0.0	0.0	20.00833803189389	0.0
32	0.0	0.0	0.0	20.314494401213604	0.0
33	0.0	0.0	0.0	20.616793971639673	0.0
34	0.0	0.0	0.0	20.935806337271554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	85	0.0	40.0	21
TCTGCGT	30	4.862788E-6	40.0	37
GAGTGTA	20	0.001255187	40.0	26
CGGGTAC	35	3.052428E-7	40.0	6
GATACTC	20	0.001255187	40.0	9
CTGTCGG	20	0.001255187	40.0	2
TCGTTCA	25	7.789243E-5	40.0	16
GCCTTAC	45	1.2187229E-9	40.0	40
GTCGCAA	20	0.001255187	40.0	20
ACACGAC	85	0.0	40.0	26
CTGTATG	20	0.001255187	40.0	21
GTATTCG	20	0.001255187	40.0	9
ATTAGCG	25	7.789243E-5	40.0	1
GGTAAGT	20	0.001255187	40.0	8
ATCTACG	30	4.862788E-6	40.0	1
TTTACGG	50	7.8216544E-11	40.0	2
GAATGCG	30	4.862788E-6	40.0	1
CGCGGCA	20	0.001255187	40.0	4
CATGCGA	55	5.456968E-12	40.0	10
TGAAGCG	20	0.001255187	40.0	24
>>END_MODULE