FastQCFastQC Report
Sat 18 Jun 2016
SRR3548890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558796
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124572.229257188669926No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30470.5452794937687456No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24370.4361162213043758No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14270.25537047509287825No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT12440.2226214933535673TruSeq Adapter, Index 13 (96% over 25bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9240.16535551435586512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA551.8189894E-1245.00000443
TCGTAAT551.8189894E-1245.00000444
TTTTCGT551.8189894E-1245.00000441
ATTTACG253.8882237E-545.01
AACGTTC207.030173E-445.039
AACCGCA207.030173E-445.010
GGTACGC406.8048394E-945.034
ACGCCTA207.030173E-445.018
CGTATCT207.030173E-445.043
GCGAAAT207.030173E-445.028
GTTTATC253.8882237E-545.041
TGGCGAT207.030173E-445.032
TTGTCGT253.8882237E-545.027
CCGTGAT207.030173E-445.045
CGATTCC253.8882237E-545.014
TATGCGT253.8882237E-545.045
GCGTTCC207.030173E-445.032
TCCTACC253.8882237E-545.044
TAGCACG351.2104283E-745.01
CCGTACT351.2104283E-745.044