FastQCFastQC Report
Sat 18 Jun 2016
SRR3548887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626265
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175422.8010506734369636No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42130.6727184179221256No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33660.5374721563555364No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT23220.3707695624056909No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19090.3048230381707424No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT9070.1448268704142815No Hit
AATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGT7970.127262420860179No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT7660.12231243962220466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC257.790586E-540.026
TCACGCG304.8639613E-640.01
TCACGCA353.0534102E-740.034
GTCATAG200.001255331240.01
GTACCTA200.001255331240.023
AATCCCG507.8216544E-1140.01
GGTACGT200.001255331240.08
AGATCTA200.001255331240.040
TCGCAAT200.001255331240.025
CAACGTT200.001255331240.034
CGAGTAC401.925946E-840.021
TACCACG507.8216544E-1140.01
CGCGGCA353.0534102E-740.026
TTGTCAC200.001255331240.012
CGACCGT200.001255331240.016
GCACGAT257.790586E-540.040
CGATTTA257.790586E-540.012
CACGATA200.001255331240.022
GAATACG257.790586E-540.01
GCGTTCC257.790586E-540.026