##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626265
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112993700749684	31.0	31.0	34.0	28.0	34.0
2	31.434223531572098	31.0	31.0	34.0	28.0	34.0
3	31.56579722641374	31.0	31.0	34.0	30.0	34.0
4	35.54658491213783	37.0	35.0	37.0	33.0	37.0
5	35.1225790999018	37.0	35.0	37.0	32.0	37.0
6	35.276504355185104	37.0	35.0	37.0	32.0	37.0
7	35.1369100939698	37.0	35.0	37.0	32.0	37.0
8	35.5966579642803	37.0	35.0	37.0	35.0	37.0
9	36.9248497041987	39.0	37.0	39.0	32.0	39.0
10	36.24798288264552	37.0	35.0	39.0	32.0	39.0
11	36.458695600105386	38.0	35.0	39.0	32.0	39.0
12	36.19344207324375	37.0	35.0	39.0	32.0	39.0
13	35.85516035544059	38.0	35.0	39.0	30.0	39.0
14	37.06814846750178	39.0	35.0	40.0	31.0	41.0
15	36.98329940201033	39.0	35.0	40.0	32.0	41.0
16	36.86685668207548	38.0	35.0	40.0	32.0	41.0
17	36.82696462360183	38.0	35.0	40.0	32.0	41.0
18	36.63564784875412	38.0	35.0	40.0	31.0	41.0
19	36.79234189999441	38.0	35.0	40.0	32.0	41.0
20	36.516841911970175	38.0	35.0	40.0	31.0	41.0
21	36.595557791030956	38.0	35.0	40.0	31.0	41.0
22	36.69322730792875	38.0	35.0	40.0	31.0	41.0
23	36.47941526350666	38.0	35.0	40.0	31.0	41.0
24	36.07028973357924	38.0	34.0	40.0	30.0	41.0
25	36.22837297310244	38.0	35.0	40.0	31.0	41.0
26	36.240513201280606	38.0	35.0	40.0	31.0	41.0
27	36.13167109769826	38.0	35.0	40.0	30.0	41.0
28	36.08992678818072	38.0	35.0	40.0	30.0	41.0
29	35.80682778057212	38.0	34.0	40.0	30.0	41.0
30	35.828962180546576	38.0	34.0	40.0	30.0	41.0
31	35.74431750137721	38.0	34.0	40.0	30.0	41.0
32	35.63257247331401	38.0	34.0	40.0	29.0	41.0
33	35.59059343887971	38.0	34.0	40.0	29.0	41.0
34	35.495525057284055	38.0	34.0	40.0	29.0	41.0
35	35.24899842718338	38.0	34.0	40.0	27.0	41.0
36	35.216833129745396	37.0	34.0	40.0	27.0	41.0
37	35.096222844961794	37.0	34.0	40.0	27.0	41.0
38	34.87920129657573	37.0	34.0	40.0	26.0	41.0
39	34.82491597007657	37.0	34.0	40.0	25.0	41.0
40	34.628543827293555	37.0	33.0	40.0	24.0	41.0
41	34.64680766129354	37.0	34.0	40.0	25.0	41.0
42	34.51362442416549	37.0	33.0	40.0	24.0	41.0
43	34.449086249431154	37.0	33.0	40.0	24.0	41.0
44	34.3544937047416	36.0	33.0	40.0	24.0	41.0
45	34.14633741307593	36.0	33.0	40.0	23.0	41.0
46	33.11346474735136	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	7.0
10	13.0
11	6.0
12	9.0
13	17.0
14	23.0
15	48.0
16	75.0
17	172.0
18	307.0
19	682.0
20	1225.0
21	2031.0
22	3140.0
23	4113.0
24	5424.0
25	6687.0
26	7610.0
27	8648.0
28	9772.0
29	11847.0
30	14810.0
31	19171.0
32	25785.0
33	35944.0
34	60007.0
35	67511.0
36	62174.0
37	84484.0
38	116930.0
39	77588.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.650683017572433	33.58386625470048	19.80711040853313	25.95834031919395
2	23.632807198230783	33.00471844985749	19.324407399423567	24.038066952488162
3	19.524003417083822	35.06039775494399	19.337820251810335	26.077778576161847
4	18.810088381116618	37.54369156826583	18.54342810152252	25.10279194909503
5	17.23615402425491	38.87858973437762	16.11506311226078	27.77019312910669
6	19.179899882637542	41.33026753850208	17.84324527157034	21.646587307290044
7	80.02474990618987	13.027552234277822	3.7011488746776524	3.246548984854654
8	81.87684127326291	11.748700629925033	3.111143046473937	3.2633150503381154
9	76.95400509369037	14.20772356749938	5.333045915067903	3.5052254237423455
10	38.24786631857121	38.27309525520347	10.34721723232178	13.131821193903539
11	30.680143389779087	31.922908034138903	20.54258181440764	16.85436676167437
12	29.571347592472836	30.161513097490676	24.100181233184035	16.16695807685245
13	23.327664806431784	36.66291426153465	21.614332590836145	18.395088341197415
14	18.820627050849083	37.28788931203245	24.97744565000439	18.91403798711408
15	18.194534262652393	32.89757530757746	30.07576664830383	18.83212378146631
16	19.72615426377013	31.819118104955567	28.777274795813273	19.677452835461025
17	20.180275123150743	31.41369867388406	24.66352103342834	23.742505169536855
18	22.049451909335506	31.651617126935083	25.423582668678595	20.875348295050816
19	23.3883419958005	34.06816603195133	22.170327257630557	20.373164714617616
20	24.608272855739983	33.55161153824659	22.391639322012246	19.44847628400118
21	21.841073666898197	34.683241119973175	23.845975745091934	19.629709468036694
22	23.465944927466804	30.583858270859782	23.085754433027553	22.86444236864586
23	21.463278324670867	34.04165968080605	22.472435789961118	22.022626204561966
24	19.98211619681764	33.22698857512395	26.28919067806759	20.50170454999082
25	20.628328263594483	33.608456484076235	23.8282516187237	21.934963633605584
26	21.878757395032455	36.405994267602374	21.538965134567636	20.17628320279754
27	19.70475757067695	35.38565942532315	23.007193440476474	21.90238956352343
28	19.379974930740183	35.93782184857848	26.148515404820643	18.5336878158607
29	19.838726417730513	34.650986403519276	24.622484092197393	20.887803086552818
30	21.381204442208972	33.68733683025557	25.22590277278788	19.70555595474759
31	26.227076397371718	32.45511085562821	20.97993660830479	20.33787613869528
32	24.804196306675287	33.00104588313254	22.799932935738067	19.394824874454105
33	24.606197057156315	32.134958843301156	21.94949422369125	21.30934987585128
34	21.239890461705507	34.25243307545528	23.670810279993294	20.83686618284592
35	22.301900952472195	33.272975497592874	25.202110927482774	19.223012622452156
36	24.724517576425313	32.454312471557564	23.436724070481347	19.384445881535772
37	23.08511572577104	32.61462799294229	22.992822527204936	21.30743375408174
38	23.96940592241304	32.92248489058146	23.059727112324655	20.048382074680845
39	22.965358115174887	30.5749163692686	24.9865472284097	21.473178287146816
40	25.076125921135624	29.770624256504835	23.838630611642035	21.31461921071751
41	21.235898541352302	28.620791517967632	26.55553160403344	23.58777833664663
42	25.38845376957039	28.828531053148428	23.441035344462808	22.341979832818375
43	23.80270332846319	28.942540298435965	25.025189017428723	22.22956735567212
44	23.295250413163757	29.583642707160706	25.87945997301462	21.241646906660918
45	23.364071120053012	31.62495109897567	24.091079654778728	20.919898126192585
46	22.326810535476195	30.153050226341882	26.899635138479717	20.620504099702202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20405.0
1	16165.0
2	11925.0
3	8208.0
4	4491.0
5	4151.5
6	3812.0
7	3812.0
8	3858.5
9	3905.0
10	3865.0
11	3825.0
12	3904.5
13	3984.0
14	3688.0
15	3392.0
16	3561.5
17	3731.0
18	3485.5
19	3240.0
20	3240.0
21	3216.5
22	3193.0
23	3353.0
24	3513.0
25	3721.0
26	3929.0
27	4516.5
28	5104.0
29	6532.5
30	7961.0
31	7961.0
32	9057.5
33	10154.0
34	12699.0
35	15244.0
36	15896.5
37	16549.0
38	20591.5
39	24634.0
40	27172.5
41	29711.0
42	31911.0
43	34111.0
44	34111.0
45	40204.0
46	46297.0
47	49071.0
48	51845.0
49	51002.5
50	50160.0
51	47144.0
52	44128.0
53	41823.0
54	39518.0
55	35956.0
56	32394.0
57	32394.0
58	30381.0
59	28368.0
60	26820.5
61	25273.0
62	25331.0
63	25389.0
64	22187.0
65	18985.0
66	16725.5
67	14466.0
68	13517.5
69	12569.0
70	12569.0
71	10549.5
72	8530.0
73	6773.5
74	5017.0
75	3591.5
76	2166.0
77	2303.0
78	2440.0
79	1853.5
80	1267.0
81	1267.0
82	727.0
83	187.0
84	162.5
85	138.0
86	162.5
87	187.0
88	128.0
89	69.0
90	52.5
91	36.0
92	23.5
93	11.0
94	11.0
95	8.5
96	6.0
97	6.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	626265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.019137927205428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1457299491539	16.986824682453623
2	9.021012049370091	3.9726981711612424
3	3.240951742713331	2.140888903146649
4	1.639394377560794	1.4439220366638483
5	1.0665797137374398	1.1742582913571986
6	0.7613598456181304	1.0058692471740867
7	0.5279107877719615	0.8136898314447347
8	0.4303269855611759	0.7580343399096057
9	0.3147132100261194	0.6236742221170825
>10	3.660786698091879	19.204278639669873
>50	1.3743365522345954	21.72745689678429
>100	0.8096303115755363	24.871249685651772
>500	0.003633888292529337	0.5799493543830881
>1k	0.00290711063402347	1.889956367346653
>5k	0.0	0.0
>10k+	7.267776585058675E-4	2.80724933073624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17542	2.8010506734369636	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4213	0.6727184179221256	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3366	0.5374721563555364	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	2322	0.3707695624056909	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1909	0.3048230381707424	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT	907	0.1448268704142815	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGT	797	0.127262420860179	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT	766	0.12231243962220466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.596768141282045E-4	0.0	0.0	0.41148715000838304	0.0
2	1.596768141282045E-4	0.0	0.0	0.5304463765338954	0.0
3	1.596768141282045E-4	0.0	0.0	0.6856522398665101	0.0
4	1.596768141282045E-4	0.0	0.0	1.4342171444995329	0.0
5	1.596768141282045E-4	0.0	0.0	1.5606811812890709	0.0
6	1.596768141282045E-4	0.0	0.0	2.268049467877017	0.0
7	1.596768141282045E-4	0.0	0.0	2.764644359815733	0.0
8	1.596768141282045E-4	0.0	0.0	3.1103446624032958	0.0
9	1.596768141282045E-4	0.0	0.0	3.717755263346986	0.0
10	1.596768141282045E-4	0.0	0.0	4.527955418233495	0.0
11	1.596768141282045E-4	0.0	0.0	5.393882781250748	0.0
12	1.596768141282045E-4	0.0	0.0	5.813673125593798	0.0
13	1.596768141282045E-4	0.0	0.0	5.994427279186926	0.0
14	1.596768141282045E-4	0.0	0.0	6.178694322690873	0.0
15	1.596768141282045E-4	0.0	0.0	6.341404996287514	0.0
16	1.596768141282045E-4	0.0	0.0	6.635849041539923	0.0
17	1.596768141282045E-4	0.0	0.0	6.960472004662563	0.0
18	1.596768141282045E-4	0.0	0.0	7.421299290236561	0.0
19	1.596768141282045E-4	0.0	0.0	7.586405116045125	0.0
20	1.596768141282045E-4	0.0	0.0	7.804044613701867	0.0
21	1.596768141282045E-4	0.0	0.0	8.023121202685765	0.0
22	1.596768141282045E-4	0.0	0.0	8.254971936799917	0.0
23	1.596768141282045E-4	0.0	0.0	8.494008127549838	0.0
24	1.596768141282045E-4	0.0	0.0	8.66550102592353	0.0
25	1.596768141282045E-4	0.0	0.0	8.81416013987689	0.0
26	1.596768141282045E-4	0.0	0.0	8.954995089937965	0.0
27	1.596768141282045E-4	0.0	0.0	9.126008957869272	0.0
28	1.596768141282045E-4	0.0	0.0	9.281534174830144	0.0
29	1.596768141282045E-4	0.0	0.0	9.436899714976887	0.0
30	1.596768141282045E-4	0.0	0.0	9.678810088381116	0.0
31	1.596768141282045E-4	0.0	0.0	9.83768851843868	0.0
32	1.596768141282045E-4	0.0	0.0	9.998004039823398	0.0
33	1.596768141282045E-4	0.0	0.0	10.162630835189576	0.0
34	1.596768141282045E-4	0.0	0.0	10.313685101354858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	25	7.790586E-5	40.0	26
TCACGCG	30	4.8639613E-6	40.0	1
TCACGCA	35	3.0534102E-7	40.0	34
GTCATAG	20	0.0012553312	40.0	1
GTACCTA	20	0.0012553312	40.0	23
AATCCCG	50	7.8216544E-11	40.0	1
GGTACGT	20	0.0012553312	40.0	8
AGATCTA	20	0.0012553312	40.0	40
TCGCAAT	20	0.0012553312	40.0	25
CAACGTT	20	0.0012553312	40.0	34
CGAGTAC	40	1.925946E-8	40.0	21
TACCACG	50	7.8216544E-11	40.0	1
CGCGGCA	35	3.0534102E-7	40.0	26
TTGTCAC	20	0.0012553312	40.0	12
CGACCGT	20	0.0012553312	40.0	16
GCACGAT	25	7.790586E-5	40.0	40
CGATTTA	25	7.790586E-5	40.0	12
CACGATA	20	0.0012553312	40.0	22
GAATACG	25	7.790586E-5	40.0	1
GCGTTCC	25	7.790586E-5	40.0	26
>>END_MODULE