##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	640943
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17701574086931	31.0	31.0	34.0	30.0	34.0
2	31.32569198821112	31.0	31.0	34.0	30.0	34.0
3	31.506171375613743	31.0	31.0	34.0	30.0	34.0
4	35.4248958175688	37.0	35.0	37.0	33.0	37.0
5	34.999056078309614	35.0	35.0	37.0	32.0	37.0
6	34.9682467863757	35.0	35.0	37.0	32.0	37.0
7	34.06279341532711	35.0	35.0	37.0	30.0	37.0
8	34.39960963767449	37.0	35.0	37.0	31.0	37.0
9	35.57037989337585	39.0	35.0	39.0	30.0	39.0
10	35.46705089220102	37.0	35.0	39.0	30.0	39.0
11	35.862678896563345	37.0	35.0	39.0	31.0	39.0
12	35.54841850211329	37.0	35.0	39.0	30.0	39.0
13	35.44677451817088	37.0	35.0	39.0	30.0	39.0
14	36.50313366399196	38.0	35.0	40.0	31.0	41.0
15	36.57845549448235	38.0	35.0	40.0	31.0	41.0
16	36.48544098305153	38.0	35.0	40.0	31.0	41.0
17	36.393512371614946	38.0	35.0	40.0	31.0	41.0
18	36.0822335215456	38.0	35.0	40.0	30.0	41.0
19	35.93970758710213	38.0	34.0	40.0	30.0	41.0
20	35.89926873372515	38.0	34.0	40.0	30.0	41.0
21	35.91135717216664	38.0	34.0	40.0	30.0	41.0
22	35.87839948326138	38.0	34.0	40.0	30.0	41.0
23	35.75843405731867	38.0	34.0	40.0	30.0	41.0
24	35.341041247037566	37.0	34.0	40.0	27.0	41.0
25	35.40035541381995	37.0	34.0	40.0	27.0	41.0
26	35.272348711195846	37.0	34.0	40.0	27.0	41.0
27	35.120152650079646	37.0	33.0	40.0	27.0	41.0
28	35.055274806027995	37.0	33.0	40.0	27.0	41.0
29	34.7863725791529	37.0	33.0	40.0	25.0	41.0
30	34.77991334642862	37.0	33.0	40.0	25.0	41.0
31	34.603673025526454	37.0	33.0	40.0	25.0	41.0
32	34.45012895062431	37.0	33.0	40.0	24.0	41.0
33	34.36297299447845	37.0	33.0	40.0	24.0	41.0
34	34.23268371758487	36.0	33.0	40.0	23.0	41.0
35	33.99237373682215	36.0	33.0	40.0	23.0	41.0
36	33.880847438851816	36.0	33.0	40.0	22.0	41.0
37	33.748695281795726	36.0	33.0	40.0	22.0	41.0
38	33.58344345753054	36.0	33.0	40.0	21.0	41.0
39	33.467085216626124	36.0	33.0	40.0	21.0	41.0
40	33.099434739126565	35.0	32.0	39.0	19.0	41.0
41	33.15846026869784	35.0	32.0	39.0	19.0	41.0
42	32.96441493237308	35.0	32.0	39.0	18.0	40.0
43	32.919204983906525	35.0	32.0	39.0	18.0	40.0
44	32.787433515928875	35.0	32.0	39.0	18.0	40.0
45	32.5655682330566	35.0	31.0	39.0	18.0	40.0
46	32.38043788605227	35.0	31.0	39.0	17.0	40.0
47	32.213435516106735	35.0	31.0	39.0	15.0	40.0
48	32.04202869833979	35.0	31.0	39.0	15.0	40.0
49	31.85700288481191	35.0	30.0	39.0	14.0	40.0
50	31.668469739118766	35.0	30.0	38.0	12.0	40.0
51	29.611871882523094	34.0	26.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	11.0
12	23.0
13	31.0
14	66.0
15	126.0
16	269.0
17	569.0
18	1280.0
19	2266.0
20	3583.0
21	4926.0
22	6555.0
23	8254.0
24	9358.0
25	10092.0
26	10909.0
27	12384.0
28	14591.0
29	17105.0
30	21165.0
31	26250.0
32	34168.0
33	44715.0
34	65930.0
35	68647.0
36	65542.0
37	81259.0
38	87919.0
39	42932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.819302184437618	37.36556916917729	18.196937949240414	25.618190697144676
2	21.619083132197403	40.310448823062266	18.335483810572857	19.73498423416747
3	18.890135316244972	36.67159170160217	21.888841909498975	22.549431072653885
4	21.23933017444609	36.38685499334574	17.28687262361864	25.08694220858953
5	15.942603320420067	42.36741800752953	16.238417456778528	25.451561215271873
6	18.311300692885325	41.733196243659734	19.87883477937976	20.076668284075183
7	71.66191065352146	19.05052399355325	5.181271969582318	4.10629338334298
8	74.38961030856099	14.5792995632997	4.016269777499716	7.014820350639604
9	70.41078535844841	18.00050862557201	6.693574935680707	4.895131080298873
10	39.48791078145795	36.432724906895	10.683321293781194	13.396043017865864
11	32.80962581696033	31.91188608035348	18.73255500099073	16.54593310169547
12	28.839850033466313	31.738391713459702	23.96078902492109	15.460969228152893
13	22.853358254946226	38.587674723025295	19.67788087240207	18.881086149626412
14	17.678951170384885	38.248643015057496	26.56258044787134	17.509825366686275
15	16.792444881994186	35.18986867786995	27.65019666335384	20.367489776782023
16	18.801203851200494	32.99528975275493	28.350726975721706	19.852779420322868
17	18.741448147495174	33.493149936889864	23.49990560783096	24.265496307783998
18	21.43482337742982	34.3336302916172	23.135910681605072	21.095635649347912
19	21.959831061420438	35.25789344762327	23.055560322836822	19.726715168119473
20	24.407786651855158	34.64535847961519	21.791641378406503	19.155213490123145
21	21.28910059084817	35.34011604776088	22.28996962288378	21.080813738507167
22	21.62391975573491	34.09538757736647	22.831515438970392	21.449177227928224
23	21.68461158012491	34.87283580599211	19.66883170578351	23.77372090809947
24	19.23259946672325	36.44770283784985	24.164395273838704	20.155302421588193
25	18.68387672538744	34.111457649120126	23.974830835191273	23.229834790301165
26	21.60706958341069	36.39403191859494	20.517581126558838	21.481317371435527
27	18.936005229794226	35.83173542733129	24.018672487257057	21.213586855617425
28	19.688022179819423	35.08127867844723	25.33361000900236	19.89708913273099
29	19.95122811232824	35.1475872269453	25.366530253080228	19.534654407646237
30	22.922319145384222	32.203643693745	23.983099901239267	20.89093725963151
31	23.70678827914495	33.808622607626575	22.047514365552008	20.43707474767647
32	25.258564334113952	32.83958167887004	20.64707782127272	21.254776165743287
33	23.706008178574383	31.916722703890986	22.64023477906772	21.737034338466916
34	21.895082714063495	32.019851999319755	24.18280564730405	21.9022596393127
35	22.980046587606072	32.18507730016554	24.800333258963743	20.034542853264643
36	22.940885538963684	31.334611658134964	25.861426055047016	19.863076747854333
37	24.129914828619707	32.16339050430381	21.561823750317892	22.144870916758588
38	22.56659328520633	32.240152400447464	23.785422416657955	21.40783189768825
39	23.67589629655055	29.08495763273801	23.960476984692868	23.27866908601857
40	23.428448395567155	31.217908612778363	21.25789656802555	24.095746423628935
41	19.646676849579446	27.44362603226808	27.70932828660271	25.200368831549763
42	25.241402121561514	30.204870011841926	21.770422642887123	22.78330522370944
43	24.273921393946107	28.484904273858984	24.924213229569556	22.316961102625353
44	22.4472378979098	29.420244857967088	24.058145576127675	24.074371667995436
45	24.464733993506442	31.070781645169692	24.217442112637162	20.2470422486867
46	21.574617399675166	28.85108348168246	27.959428529526026	21.61487058911635
47	23.555760808683456	30.260881232808533	23.63673524790816	22.546622710599852
48	22.28716126082975	27.067305517027258	28.339493527505567	22.306039694637434
49	23.03246934594808	27.198206392768153	27.06371705440265	22.705607206881112
50	20.899050305565396	29.191363350563154	24.72185514156485	25.1877312023066
51	20.359688771076367	28.088301143783458	28.432013455174644	23.119996629965538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20949.0
1	16526.5
2	12104.0
3	8203.0
4	4302.0
5	4014.0
6	3726.0
7	3934.5
8	4143.0
9	4596.0
10	5049.0
11	5209.0
12	5369.0
13	5282.5
14	5196.0
15	5025.0
16	4854.0
17	4551.5
18	4249.0
19	3768.0
20	3287.0
21	3168.5
22	3050.0
23	3024.0
24	2998.0
25	3614.5
26	4022.0
27	3813.0
28	4776.0
29	5739.0
30	7030.0
31	8321.0
32	9390.0
33	10459.0
34	11539.5
35	12620.0
36	13424.5
37	14229.0
38	15983.5
39	17738.0
40	20122.0
41	22506.0
42	26027.5
43	29549.0
44	42903.0
45	56257.0
46	55032.0
47	53807.0
48	49586.5
49	45366.0
50	43106.0
51	40846.0
52	38726.0
53	36606.0
54	33595.0
55	30584.0
56	28486.0
57	26388.0
58	26451.0
59	26514.0
60	25562.0
61	24610.0
62	24163.5
63	23717.0
64	21072.5
65	18428.0
66	16648.5
67	14869.0
68	12756.5
69	10644.0
70	8977.0
71	7310.0
72	6496.5
73	5683.0
74	4799.5
75	3306.5
76	2697.0
77	2188.0
78	1679.0
79	1304.5
80	930.0
81	874.5
82	819.0
83	623.0
84	427.0
85	289.5
86	152.0
87	132.0
88	112.0
89	82.0
90	52.0
91	39.5
92	27.0
93	17.5
94	8.0
95	7.0
96	6.0
97	4.5
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	640943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.243780387721888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3810810758675	22.17128300546324
2	7.93053735226146	4.321156359632733
3	2.81304418055901	2.2991387362832625
4	1.4084977046951537	1.534912085733005
5	0.8333644514240122	1.1351999048765056
6	0.5647971171985664	0.9232325174745698
7	0.4374678413927427	0.8342794458316243
8	0.29170968488040727	0.6357819673482697
9	0.24145518616945824	0.5920336858929502
>10	2.5998412577565677	16.614502504083696
>50	0.9463266042909014	18.39511834113426
>100	0.5425137675804982	21.56303983333778
>500	0.0035114159713948104	0.6251658448301374
>1k	0.0035114159713948104	2.4507585312124562
>5k	0.0017557079856974052	3.0909117745159507
>10k+	5.852359952324683E-4	2.8134854623495547
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17646	2.753130933639965	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT	6830	1.0656173793925514	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	6577	1.0261442905219342	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	5979	0.9328442622822934	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4069	0.6348458443262506	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3468	0.5410777557442705	No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	2783	0.43420397757678925	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2323	0.36243472508475794	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT	1520	0.23715057345192941	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCT	1208	0.18847229784863864	No Hit
AACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	946	0.14759502795100343	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTAT	730	0.11389468330257135	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5936565342003891	0.0
2	0.0	0.0	0.0	0.6963177692868164	0.0
3	0.0	0.0	0.0	1.015066862419903	0.0
4	0.0	0.0	0.0	2.7985327868468803	0.0
5	0.0	0.0	0.0	3.000734854737473	0.0
6	0.0	0.0	0.0	3.924685970515319	0.0
7	0.0	0.0	0.0	4.667965794150182	0.0
8	0.0	0.0	0.0	5.038201524940595	0.0
9	0.0	0.0	0.0	6.788903225403819	0.0
10	0.0	0.0	0.0	7.537487732918528	0.0
11	0.0	0.0	0.0	9.426735294714195	0.0
12	0.0	0.0	0.0	9.860939272290985	0.0
13	0.0	0.0	0.0	10.092785161863068	0.0
14	0.0	0.0	0.0	10.411378234882042	0.0
15	0.0	0.0	0.0	10.568022429451604	0.0
16	0.0	0.0	0.0	10.82951214070518	0.0
17	0.0	0.0	0.0	11.10083111914788	0.0
18	0.0	0.0	0.0	11.522709507709735	0.0
19	0.0	0.0	0.0	11.683254205132126	0.0
20	0.0	0.0	0.0	11.86252131624809	0.0
21	0.0	0.0	0.0	12.050681573868504	0.0
22	0.0	0.0	0.0	12.236813570005445	0.0
23	0.0	0.0	0.0	12.42965443104925	0.0
24	0.0	0.0	0.0	12.564611829757093	0.0
25	0.0	0.0	0.0	12.685995478537093	0.0
26	0.0	0.0	0.0	12.802386483665474	0.0
27	0.0	0.0	0.0	12.924862273244267	0.0
28	0.0	0.0	0.0	13.05435896795815	0.0
29	0.0	0.0	0.0	13.19430901031761	0.0
30	0.0	0.0	0.0	13.38480956964972	0.0
31	0.0	0.0	0.0	13.510405761510773	0.0
32	0.0	0.0	0.0	13.632101450519	0.0
33	0.0	0.0	0.0	13.75442121998368	0.0
34	0.0	0.0	0.0	13.882357713556432	0.0
35	0.0	0.0	0.0	14.020435514546536	0.0
36	0.0	0.0	0.0	14.142287223668875	0.0
37	0.0	0.0	0.0	14.267415355187591	0.0
38	0.0	0.0	0.0	14.392387466592194	0.0
39	0.0	0.0	0.0	14.535457911233916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAC	35	1.2108103E-7	45.000004	34
ATCCGGT	35	1.2108103E-7	45.000004	13
ATATAAG	35	1.2108103E-7	45.000004	1
TATTCGG	65	0.0	45.000004	2
ACTACCC	35	1.2108103E-7	45.000004	42
ATGAACG	35	1.2108103E-7	45.000004	1
ATAGTCG	35	1.2108103E-7	45.000004	1
CGCAATG	35	1.2108103E-7	45.000004	1
GCGCTTA	35	1.2108103E-7	45.000004	34
GTAACGA	35	1.2108103E-7	45.000004	37
ACGACAG	35	1.2108103E-7	45.000004	1
AGACTCG	20	7.030948E-4	45.0	1
GATACGT	25	3.8888676E-5	45.0	2
ATCTCGA	20	7.030948E-4	45.0	44
GTCGCAT	20	7.030948E-4	45.0	42
ACGCCCA	20	7.030948E-4	45.0	10
GATCGCG	20	7.030948E-4	45.0	9
CACGGTA	20	7.030948E-4	45.0	10
ATAGGCC	20	7.030948E-4	45.0	1
TTTACGG	25	3.8888676E-5	45.0	2
>>END_MODULE