FastQCFastQC Report
Sat 18 Jun 2016
SRR3548883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671794
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224353.3395654024894537No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTAT59410.8843484758720679No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54760.8151308287957321No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGT52760.7853597977951574No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT48420.7207566605239105No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42800.6371000634122961No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT38960.5799396838911929No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28800.42870284640827394No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC13220.19678651491379798No Hit
AACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTC10850.1615078431781171No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC10380.15451165089298208No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTG8030.11953068946730695No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT7830.1165535863672495No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTGCCAT7570.11268335233717479No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC6880.10241234664197654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAT200.001255396240.00000434
CTTCGAC200.001255396240.00000415
TCGTTCG200.001255396240.0000041
ATCTCGC401.9263098E-840.00000414
GTCGATT200.001255396240.00000432
GTCGATA200.001255396240.00000414
CTACTCT200.001255396240.00000428
GGTCGTA200.001255396240.0000049
CTCAACT200.001255396240.00000423
CACGGTA257.791189E-540.00000410
CGAGTCG257.791189E-540.00000412
CTCCGAA200.001255396240.00000421
ACGTTCA257.791189E-540.0000041
ACGTTAT257.791189E-540.00000410
CCTAGCG200.001255396240.0000041
TAGACGT257.791189E-540.00000414
TAGACGA257.791189E-540.00000433
TCCGAAC200.001255396240.00000422
GACCGTA257.791189E-540.0000049
CGCATCT200.001255396240.00000413
Produced by FastQC (version 0.11.5)