FastQCFastQC Report
Sat 18 Jun 2016
SRR3548882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355874
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161064.525759116990844No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36771.0332308626086761No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGT34530.9702872364938152No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31700.8907647088576294No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC29850.8387800176466951No Hit
AATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT27640.776679386524444No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT23750.6673710358160473No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18690.5251858803958704No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT7820.21974069474027325No Hit
AAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT5720.16073104525759116No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT5370.15089610367714418No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.14836711869931493No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCC5110.1435901470745264No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4730.13291221050146962No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT4140.11633330898014466No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCGT3920.11015134570100654No Hit
AACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG3920.11015134570100654No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT3800.10677936573056757No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3690.10368838409099851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA302.1612614E-645.00000444
ATCCTCG302.1612614E-645.00000416
TCGTTGA900.045.00000424
CCTTACT302.1612614E-645.00000411
GCCTTAC302.1612614E-645.00000410
TCGCAAT302.1612614E-645.00000414
CTCGTGT302.1612614E-645.00000419
GAAGACG302.1612614E-645.0000041
CGACGCG453.8380676E-1045.0000041
GCAACCG302.1612614E-645.0000041
ATAGCGG302.1612614E-645.0000042
GTCTCCG302.1612614E-645.00000410
GGATCCA302.1612614E-645.0000048
TACGGTA302.1612614E-645.00000414
GCGTTTC302.1612614E-645.0000049
TCAGGCG302.1612614E-645.0000041
TAGTTAG302.1612614E-645.00000442
TATTGAG302.1612614E-645.00000430
TGCGACT302.1612614E-645.00000433
GAAACGC302.1612614E-645.00000430
Produced by FastQC (version 0.11.5)