##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355874
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.037766175668917	31.0	31.0	33.0	28.0	34.0
2	31.20058784850818	31.0	31.0	34.0	28.0	34.0
3	31.411547345408767	31.0	31.0	34.0	30.0	34.0
4	35.337141797377726	37.0	35.0	37.0	33.0	37.0
5	34.88847738244435	35.0	35.0	37.0	32.0	37.0
6	34.85397640738014	35.0	35.0	37.0	32.0	37.0
7	33.94003214620905	35.0	35.0	37.0	30.0	37.0
8	34.32526680791516	35.0	35.0	37.0	31.0	37.0
9	35.50632246244457	38.0	35.0	39.0	30.0	39.0
10	35.29726813422728	37.0	35.0	39.0	30.0	39.0
11	35.70772520611228	37.0	35.0	39.0	30.0	39.0
12	35.26375627328774	37.0	35.0	39.0	30.0	39.0
13	34.88646824437863	37.0	33.0	39.0	27.0	39.0
14	35.915427932358085	38.0	34.0	40.0	29.0	41.0
15	36.19702197968944	38.0	34.0	40.0	30.0	41.0
16	36.17330853054733	38.0	34.0	40.0	31.0	41.0
17	36.13217318489128	38.0	34.0	40.0	31.0	41.0
18	35.77796354889652	37.0	34.0	40.0	30.0	41.0
19	35.539646054502434	36.0	34.0	40.0	29.0	41.0
20	35.43765490032989	36.0	34.0	40.0	29.0	41.0
21	35.514187605725624	36.0	34.0	40.0	30.0	41.0
22	35.440256944873745	36.0	33.0	40.0	30.0	41.0
23	35.29984769890467	36.0	34.0	40.0	29.0	41.0
24	34.868560220752286	36.0	33.0	39.0	27.0	41.0
25	34.97336978818346	36.0	33.0	39.0	27.0	41.0
26	34.74084647937191	36.0	33.0	39.0	27.0	41.0
27	34.56571707964055	35.0	33.0	39.0	25.0	41.0
28	34.50573798591636	36.0	33.0	39.0	25.0	41.0
29	34.16563446613127	35.0	33.0	39.0	24.0	41.0
30	34.19057306799598	36.0	33.0	39.0	24.0	40.0
31	33.93075920129034	35.0	33.0	39.0	23.0	40.0
32	33.64375874607305	35.0	33.0	39.0	22.0	40.0
33	33.563868110623424	35.0	33.0	39.0	22.0	40.0
34	33.32638799125533	35.0	33.0	39.0	21.0	40.0
35	33.0935780641463	35.0	32.0	39.0	21.0	40.0
36	32.94310907793208	35.0	31.0	39.0	20.0	40.0
37	32.61464450901162	35.0	31.0	39.0	18.0	40.0
38	32.58699708323733	35.0	31.0	39.0	18.0	40.0
39	32.31964965128107	35.0	31.0	39.0	16.0	40.0
40	32.06915087924378	35.0	30.0	39.0	15.0	40.0
41	32.27342823583628	35.0	31.0	39.0	15.0	40.0
42	32.03840966184661	35.0	31.0	39.0	15.0	40.0
43	32.04321473330448	35.0	31.0	39.0	15.0	40.0
44	31.958988293609536	35.0	31.0	39.0	15.0	40.0
45	31.656465490594986	35.0	30.0	39.0	13.0	40.0
46	31.534464445281195	35.0	30.0	38.0	12.0	40.0
47	31.412997296796057	35.0	30.0	38.0	12.0	40.0
48	31.310952752940647	35.0	29.0	38.0	10.0	40.0
49	31.223663431439217	35.0	29.0	38.0	10.0	40.0
50	30.982207185689315	35.0	29.0	38.0	10.0	40.0
51	29.147392054491196	33.0	24.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	4.0
12	6.0
13	9.0
14	14.0
15	44.0
16	135.0
17	329.0
18	692.0
19	1374.0
20	2162.0
21	3162.0
22	4343.0
23	5654.0
24	6193.0
25	6865.0
26	7634.0
27	8450.0
28	9561.0
29	11247.0
30	13315.0
31	16075.0
32	20588.0
33	27019.0
34	41857.0
35	41351.0
36	31063.0
37	36781.0
38	39459.0
39	20478.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.474162203476514	40.29319365842967	19.191062005091688	23.04158213300213
2	18.72432377751676	43.44543293412837	18.375604848907198	19.45463843944767
3	16.25575344082456	40.42807285724723	21.82514035866627	21.491033343261943
4	19.605815541455684	40.0799159252994	17.08891349185386	23.225355041391055
5	13.716933521414884	47.03518661099153	16.45301426909524	22.794865598498344
6	16.75958344807432	44.71610738632212	20.44375256410977	18.080556601493786
7	67.08245053024385	23.49342744904095	5.165030319719901	4.259091700995296
8	69.77834851660981	18.68947998448889	3.8457431562856517	7.686428342615645
9	66.57749652967061	21.958052569167737	7.114877737626238	4.349573163535409
10	36.81274833227491	42.098326935938005	9.502801553358774	11.58612317842832
11	30.840971804627483	32.786042250909034	22.632167564924664	13.740818379538824
12	29.975777943879013	32.69499879170718	24.873691250273975	12.455532014139836
13	19.294469390851816	47.00174780961801	18.147434204240824	15.556348595289343
14	12.11383804380202	47.14280897171471	26.784479900189396	13.958873084293879
15	10.63016685680887	39.648302489083214	32.37297470453026	17.34855594957766
16	11.263818092920529	36.34713409802346	33.71923770772802	18.669810101328
17	12.47492089896986	35.94643047820296	24.505583436834385	27.073065185992796
18	15.474016084344457	38.45040660458477	25.789183812248158	20.286393498822616
19	17.91729657125837	41.511883419412484	23.059847024508677	17.51097298482047
20	20.391205876237095	41.35199536914751	21.19598509584853	17.060813658766865
21	14.604888246963812	40.812759572208144	23.33803537206989	21.244316808758157
22	16.101204358846108	40.24458094718917	21.247407790397723	22.40680690356699
23	15.5782664650972	40.43481681718811	18.76310154717681	25.22381517053789
24	13.277171133603465	41.97637366034046	27.712055390390983	17.034399815665093
25	13.603691194074308	38.30822144916459	25.81138268038688	22.276704676374223
26	16.416484486082155	44.820919763736605	19.402372749905865	19.36022300027538
27	12.186335613166458	43.736547204909606	23.7339620202656	20.343155161658338
28	16.496850008710947	39.86720018883088	26.107835919454637	17.528113883003535
29	12.431085159354154	40.44633774875378	27.247003152801273	19.875573939090803
30	16.632010205859377	41.18002439065512	21.79479253893232	20.393172864553186
31	20.795562474358903	40.009947340912795	18.814243243395133	20.38024694133317
32	19.77525753497024	38.499862310817875	21.223522932273784	20.5013572219381
33	21.49777730320282	40.48792550172252	19.48723424582858	18.52706294924608
34	15.453222207860085	38.504358284111795	20.985798344357836	25.05662116367029
35	17.733523662869445	36.57530474268983	24.2616768856393	21.429494708801432
36	21.512108218077184	33.22749062870567	24.912469019934022	20.347932133283127
37	15.557472588612823	39.184936241478724	22.859776212929294	22.397814956979158
38	17.694464894878525	38.620129596430196	22.016219223657814	21.66918628503347
39	17.107740380022143	37.2367748135576	24.719423166626388	20.93606163979386
40	19.19162400175343	36.291496428511216	22.22022401186937	22.296655557865986
41	16.719119688429053	29.449749068490533	29.15076684444494	24.680364398635472
42	20.90543282172904	31.211608603044898	23.996133462967233	23.886825112258833
43	18.40145669534723	32.181333842876974	26.153076650724692	23.264132811051102
44	16.864957822150537	34.12640428915852	24.287247733748462	24.72139015494248
45	19.620146456330048	39.512299296942174	21.539365056171565	19.32818919055621
46	18.04009284184852	34.840983044560716	28.655928783783025	18.46299532980774
47	19.28660143758746	35.774459499710574	22.665044369636444	22.273894693065525
48	19.054215817958042	29.159196794371038	30.141566959092263	21.645020428578654
49	19.78368748489634	28.80429590248234	28.090560142072757	23.321456470548565
50	18.188178962216963	33.1412241411286	23.270033775999373	25.40056312065506
51	16.541247744988393	33.571432585690445	27.268078027616514	22.619241641704647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18511.0
1	14531.5
2	10552.0
3	6851.0
4	3150.0
5	2755.0
6	2360.0
7	2428.0
8	2496.0
9	2588.5
10	2681.0
11	2908.0
12	3135.0
13	3200.5
14	3266.0
15	3435.0
16	3604.0
17	3434.5
18	3265.0
19	3210.5
20	3156.0
21	2990.0
22	2824.0
23	2640.0
24	2456.0
25	2948.5
26	3227.0
27	3013.0
28	3402.0
29	3791.0
30	4273.0
31	4755.0
32	5944.5
33	7134.0
34	7836.0
35	8538.0
36	8972.5
37	9407.0
38	10084.0
39	10761.0
40	13359.5
41	15958.0
42	19627.0
43	23296.0
44	30635.5
45	37975.0
46	37301.5
47	36628.0
48	33511.0
49	30394.0
50	28253.5
51	26113.0
52	23044.0
53	19975.0
54	17010.0
55	14045.0
56	11612.5
57	9180.0
58	8469.0
59	7758.0
60	7208.5
61	6659.0
62	5930.5
63	5202.0
64	5097.5
65	4993.0
66	3770.0
67	2547.0
68	1883.0
69	1219.0
70	955.0
71	691.0
72	597.0
73	503.0
74	361.0
75	156.5
76	94.0
77	64.5
78	35.0
79	35.5
80	36.0
81	37.5
82	39.0
83	24.0
84	9.0
85	6.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	2.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	355874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.826942924039727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25106925565736	23.93644062281651
2	8.911635991034277	5.316137161287951
3	2.965523013574317	2.6535745699742233
4	1.538062930043627	1.835028609119703
5	0.8996524747408888	1.3416941507783793
6	0.5844054285156698	1.0458616416501516
7	0.4088760693983822	0.8536866229465877
8	0.3141354097675604	0.7495759150045485
9	0.30715444263378205	0.8245330226372747
>10	2.69757360039346	18.232231450588042
>50	0.735973868061874	15.89248541152778
>100	0.3729732068497844	15.620318158696023
>500	0.004986272818847386	0.8715301101607065
>1k	0.006980781946386339	6.036164001874136
>5k	0.0	0.0
>10k+	9.97254563769477E-4	4.790738550938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16106	4.525759116990844	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3677	1.0332308626086761	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGT	3453	0.9702872364938152	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3170	0.8907647088576294	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC	2985	0.8387800176466951	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	2764	0.776679386524444	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	2375	0.6673710358160473	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1869	0.5251858803958704	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	782	0.21974069474027325	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	572	0.16073104525759116	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	537	0.15089610367714418	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.14836711869931493	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCC	511	0.1435901470745264	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	473	0.13291221050146962	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	414	0.11633330898014466	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCGT	392	0.11015134570100654	No Hit
AACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	392	0.11015134570100654	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT	380	0.10677936573056757	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	369	0.10368838409099851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8099833086991466E-4	0.0	0.0	1.0478427758139117	0.0
2	2.8099833086991466E-4	0.0	0.0	1.2347066658424048	0.0
3	2.8099833086991466E-4	0.0	0.0	1.6092774408920012	0.0
4	2.8099833086991466E-4	0.0	0.0	3.9207697106279187	0.0
5	2.8099833086991466E-4	0.0	0.0	4.163552268499525	0.0
6	2.8099833086991466E-4	0.0	0.0	5.359200166351012	0.0
7	2.8099833086991466E-4	0.0	0.0	6.301949566419576	0.0
8	2.8099833086991466E-4	0.0	0.0	6.798473617066715	0.0
9	2.8099833086991466E-4	0.0	0.0	9.177405486211413	0.0
10	2.8099833086991466E-4	0.0	0.0	10.287067894816705	0.0
11	2.8099833086991466E-4	0.0	0.0	12.780647082956328	0.0
12	2.8099833086991466E-4	0.0	0.0	13.382826506010554	0.0
13	2.8099833086991466E-4	0.0	0.0	13.666915818520039	0.0
14	2.8099833086991466E-4	0.0	0.0	14.045420570201813	0.0
15	2.8099833086991466E-4	0.0	0.0	14.25616931835425	0.0
16	2.8099833086991466E-4	0.0	0.0	14.652938961542569	0.0
17	2.8099833086991466E-4	0.0	0.0	15.154520982145366	0.0
18	2.8099833086991466E-4	0.0	0.0	15.820206027976194	0.0
19	2.8099833086991466E-4	0.0	0.0	16.070856539112157	0.0
20	2.8099833086991466E-4	0.0	0.0	16.364780793202087	0.0
21	2.8099833086991466E-4	0.0	0.0	16.65111809235853	0.0
22	2.8099833086991466E-4	0.0	0.0	16.964150232947617	0.0
23	2.8099833086991466E-4	0.0	0.0	17.28898430343324	0.0
24	2.8099833086991466E-4	0.0	0.0	17.51265897480569	0.0
25	2.8099833086991466E-4	0.0	0.0	17.702051849812012	0.0
26	2.8099833086991466E-4	0.0	0.0	17.888353743178765	0.0
27	2.8099833086991466E-4	0.0	0.0	18.093482524713803	0.0
28	2.8099833086991466E-4	0.0	0.0	18.285966381359696	0.0
29	2.8099833086991466E-4	0.0	0.0	18.476483249689498	0.0
30	2.8099833086991466E-4	0.0	0.0	18.743993660677656	0.0
31	2.8099833086991466E-4	0.0	0.0	18.943502475595295	0.0
32	2.8099833086991466E-4	0.0	0.0	19.135143337248575	0.0
33	2.8099833086991466E-4	0.0	0.0	19.32987518054143	0.0
34	2.8099833086991466E-4	0.0	0.0	19.51645807223905	0.0
35	2.8099833086991466E-4	0.0	0.0	19.740413741942373	0.0
36	2.8099833086991466E-4	0.0	0.0	19.953129478410897	0.0
37	2.8099833086991466E-4	0.0	0.0	20.155729274968106	0.0
38	2.8099833086991466E-4	0.0	0.0	20.36985000309098	0.0
39	2.8099833086991466E-4	0.0	0.0	20.56851582301601	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTATA	30	2.1612614E-6	45.000004	44
ATCCTCG	30	2.1612614E-6	45.000004	16
TCGTTGA	90	0.0	45.000004	24
CCTTACT	30	2.1612614E-6	45.000004	11
GCCTTAC	30	2.1612614E-6	45.000004	10
TCGCAAT	30	2.1612614E-6	45.000004	14
CTCGTGT	30	2.1612614E-6	45.000004	19
GAAGACG	30	2.1612614E-6	45.000004	1
CGACGCG	45	3.8380676E-10	45.000004	1
GCAACCG	30	2.1612614E-6	45.000004	1
ATAGCGG	30	2.1612614E-6	45.000004	2
GTCTCCG	30	2.1612614E-6	45.000004	10
GGATCCA	30	2.1612614E-6	45.000004	8
TACGGTA	30	2.1612614E-6	45.000004	14
GCGTTTC	30	2.1612614E-6	45.000004	9
TCAGGCG	30	2.1612614E-6	45.000004	1
TAGTTAG	30	2.1612614E-6	45.000004	42
TATTGAG	30	2.1612614E-6	45.000004	30
TGCGACT	30	2.1612614E-6	45.000004	33
GAAACGC	30	2.1612614E-6	45.000004	30
>>END_MODULE