##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556980
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941444935186183	31.0	31.0	34.0	28.0	34.0
2	31.254547739595676	31.0	31.0	34.0	28.0	34.0
3	31.4306581923947	31.0	31.0	34.0	28.0	34.0
4	35.425320478293656	37.0	35.0	37.0	33.0	37.0
5	34.976358217530255	35.0	35.0	37.0	32.0	37.0
6	35.11363244640741	36.0	35.0	37.0	32.0	37.0
7	34.99506265934145	37.0	35.0	37.0	32.0	37.0
8	35.42883766023915	37.0	35.0	37.0	33.0	37.0
9	36.79923516104707	39.0	37.0	39.0	32.0	39.0
10	35.99722252145499	37.0	35.0	39.0	31.0	39.0
11	36.205097130956226	37.0	35.0	39.0	32.0	39.0
12	35.846868828324176	37.0	35.0	39.0	31.0	39.0
13	35.31571151567381	37.0	35.0	39.0	29.0	39.0
14	36.49398901217279	38.0	35.0	40.0	31.0	41.0
15	36.56382814463715	38.0	35.0	40.0	31.0	41.0
16	36.482520018672126	38.0	35.0	40.0	31.0	41.0
17	36.49727638335308	38.0	35.0	40.0	31.0	41.0
18	36.24450429099788	38.0	35.0	40.0	31.0	41.0
19	36.35806851233438	38.0	35.0	40.0	31.0	41.0
20	36.10301267550002	37.0	34.0	40.0	30.0	41.0
21	36.20189773420949	38.0	34.0	40.0	31.0	41.0
22	36.277336708678945	38.0	35.0	40.0	31.0	41.0
23	36.09651693058997	38.0	34.0	40.0	31.0	41.0
24	35.67137599195662	37.0	34.0	40.0	30.0	41.0
25	35.86787317318396	37.0	34.0	40.0	30.0	41.0
26	35.79331753384323	37.0	34.0	40.0	30.0	41.0
27	35.704946317641564	37.0	34.0	40.0	30.0	41.0
28	35.70229810765198	37.0	34.0	40.0	30.0	41.0
29	35.425625695716185	37.0	34.0	40.0	29.0	41.0
30	35.45845093181084	37.0	34.0	40.0	29.0	41.0
31	35.34587776939926	37.0	34.0	40.0	29.0	41.0
32	35.22839060648497	37.0	34.0	40.0	28.0	41.0
33	35.229334985098205	37.0	34.0	40.0	28.0	41.0
34	35.166646917304035	37.0	34.0	40.0	28.0	41.0
35	34.930834141261805	37.0	34.0	40.0	27.0	41.0
36	34.865221372401166	37.0	34.0	40.0	26.0	41.0
37	34.74652770296959	37.0	33.0	40.0	25.0	41.0
38	34.54419907357535	36.0	33.0	40.0	25.0	41.0
39	34.50502890588531	36.0	33.0	40.0	24.0	41.0
40	34.32831699522424	36.0	33.0	40.0	23.0	41.0
41	34.40896800603253	36.0	33.0	40.0	24.0	41.0
42	34.27734927645517	36.0	33.0	40.0	23.0	41.0
43	34.19753492046393	36.0	33.0	40.0	23.0	41.0
44	34.08968006032533	36.0	33.0	40.0	23.0	41.0
45	33.90825882437431	35.0	33.0	40.0	23.0	41.0
46	32.901973140866815	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	0.0
10	5.0
11	18.0
12	10.0
13	12.0
14	17.0
15	32.0
16	83.0
17	170.0
18	281.0
19	623.0
20	1109.0
21	2016.0
22	2915.0
23	3940.0
24	5115.0
25	6183.0
26	7185.0
27	8118.0
28	9430.0
29	11756.0
30	14671.0
31	19325.0
32	25956.0
33	36569.0
34	61281.0
35	68498.0
36	53914.0
37	69637.0
38	91377.0
39	56731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.74070882257891	35.55262307443714	20.35082049624762	25.355847606736326
2	20.70702718230457	36.4887428632985	19.91040970950483	22.893820244892098
3	19.350066429674314	37.05339509497648	19.958885417788792	23.637653057560414
4	18.138173722575317	38.74106790189953	17.908003878056665	25.21275449746849
5	16.64458328844842	41.04115049014327	16.250673273726164	26.06359294768214
6	18.87500448849151	40.62785019210743	19.39207871018708	21.105066609213978
7	77.68519515961076	14.606987683579304	4.129412187152142	3.5784049696577975
8	79.90610075765736	12.735466264497827	3.2225573629214694	4.135875614923337
9	74.74397644439657	16.0759811842436	6.023016984451866	3.1570253869079683
10	37.576394125462315	42.21928974110381	9.674853675176847	10.52946245825703
11	30.2456102553054	31.342597579805382	24.59190635211318	13.819885812776041
12	31.155876333081977	30.323889547200977	24.7314086681748	13.788825451542246
13	20.73323997270997	41.154440015799494	21.800603253258643	16.311716758231892
14	15.555136629681495	43.273008007468846	24.982405113289527	16.189450249560128
15	14.101763079464252	35.88566914431398	33.19634457251607	16.8162232037057
16	15.71959495852634	33.80642033825272	32.748931739021145	17.72505296419979
17	16.88785952816977	30.75999138209631	25.094258321663254	27.25789076807067
18	19.703041401845667	34.081295558188806	25.912061474379687	20.303601565585836
19	20.523178570146143	37.12000430895185	23.620237710510253	18.736579410391755
20	23.440698050199288	35.96197349994614	22.47477467772631	18.122553772128263
21	19.059212179970555	35.32119645229631	25.16409924952422	20.455492118208912
22	18.764228518079644	33.955079176990196	23.51161621602212	23.76907608890804
23	18.41699881503824	36.956802757729186	21.671693777155372	22.9545046500772
24	17.691838127042264	36.06467018564401	27.23024166038278	19.01325002693095
25	17.130597148910194	35.560881898811445	24.860856763259005	22.447664189019353
26	18.898883263312864	41.14959244497109	21.15426047613918	18.797263815576862
27	14.950447053754173	38.45182950913857	24.68867822902079	21.909045208086468
28	16.09662824517936	38.34841466479945	27.83995834679881	17.714998743222377
29	16.11709576645481	36.65320119214334	26.539911666487125	20.68979137491472
30	19.21990017594887	36.690724981148335	24.677367230421197	19.412007612481595
31	22.99759416855183	36.76972243168516	20.769686523753098	19.46299687600991
32	22.705124061905273	37.21174907537075	21.387662034543432	18.695464828180544
33	22.922905669862473	35.0719954037847	21.994147007073863	20.01095191927897
34	18.959208589177347	35.842220546518725	23.317354303565658	21.881216560738267
35	19.85672735107185	36.10793924377895	24.215770763761714	19.819562641387485
36	22.770117418937843	32.52684117921649	25.934863011239184	18.768178390606487
37	19.217925239685446	36.309562282308164	23.736758950052067	20.735753527954326
38	18.51000035907932	35.101619447736006	24.21164135157456	22.17673884161011
39	19.89945779022586	34.52870839168372	26.20848145355309	19.363352364537327
40	21.729326008115194	33.17426119429782	23.89547200976696	21.20094078782003
41	18.53836762540845	30.353693130812598	27.757011023735146	23.350928220043805
42	24.460124241444937	31.15102876225358	21.664512190742936	22.72433480555855
43	21.65230349384179	30.93109267837265	24.810585658371934	22.606018169413623
44	18.734963553448956	34.6150669682933	23.412689863190778	23.23727961506697
45	20.59104456174369	37.0323889547201	22.139933211246365	20.23663327228985
46	20.4438220402887	33.94484541635247	26.488742863298505	19.122589680060326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23704.0
1	18525.5
2	13347.0
3	8670.5
4	3994.0
5	3489.5
6	2985.0
7	2985.0
8	3022.5
9	3060.0
10	3035.0
11	3010.0
12	3242.0
13	3474.0
14	3663.5
15	3853.0
16	3695.5
17	3538.0
18	3810.0
19	4082.0
20	4082.0
21	3637.5
22	3193.0
23	3441.5
24	3690.0
25	4175.0
26	4660.0
27	5019.5
28	5379.0
29	6853.5
30	8328.0
31	8328.0
32	8503.0
33	8678.0
34	10755.0
35	12832.0
36	14546.5
37	16261.0
38	18234.5
39	20208.0
40	26526.0
41	32844.0
42	37588.5
43	42333.0
44	42333.0
45	48067.0
46	53801.0
47	57194.0
48	60587.0
49	56121.5
50	51656.0
51	44805.0
52	37954.0
53	34331.0
54	30708.0
55	26972.5
56	23237.0
57	23237.0
58	21720.0
59	20203.0
60	18325.5
61	16448.0
62	14945.0
63	13442.0
64	12011.5
65	10581.0
66	8294.0
67	6007.0
68	4534.5
69	3062.0
70	3062.0
71	2803.0
72	2544.0
73	1987.0
74	1430.0
75	1285.0
76	1140.0
77	791.5
78	443.0
79	304.5
80	166.0
81	166.0
82	108.0
83	50.0
84	36.0
85	22.0
86	30.0
87	38.0
88	20.5
89	3.0
90	3.0
91	3.0
92	2.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	556980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.494713743345937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8997428257603	18.836371874539186
2	9.601518721887773	4.703729051880354
3	3.453287958000934	2.5376190001372945
4	1.7905821409016247	1.7543918790133124
5	1.1119651317885364	1.3618633797871067
6	0.7854595106239368	1.154376352183315
7	0.5371500305380074	0.9210135364680614
8	0.44296783542522156	0.86802962610003
9	0.3254002974067355	0.7173528423279856
>10	3.492573358880767	19.182159095140687
>50	0.8997675135483307	15.80748325474447
>100	0.6500361220328756	24.160921612124714
>500	0.003672520463462574	0.6018145876682516
>1k	0.004407024556155089	2.7302051921870607
>5k	7.345040926925149E-4	0.938974688502423
>10k+	7.345040926925149E-4	3.7236940271957555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20697	3.715932349455995	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5219	0.9370174871629142	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	4120	0.7397034004811662	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3906	0.7012819131746203	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2103	0.37757190563395454	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTAT	1890	0.3393299579877195	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGT	1681	0.3018061689827283	No Hit
AATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	1475	0.26482099895867	No Hit
AAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	921	0.16535602714639663	No Hit
GCCAACGGGGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAT	712	0.12783223814140543	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	620	0.11131458939279686	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	557	0.10000359079320623	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.181586412438508E-4	0.0	0.0	0.851736148515207	0.0
2	7.181586412438508E-4	0.0	0.0	1.063952027002765	0.0
3	7.181586412438508E-4	0.0	0.0	1.3266185500377032	0.0
4	7.181586412438508E-4	0.0	0.0	2.695249380588172	0.0
5	7.181586412438508E-4	0.0	0.0	2.9002836726632912	0.0
6	7.181586412438508E-4	0.0	0.0	3.8153973212682684	0.0
7	7.181586412438508E-4	0.0	0.0	4.54684189737513	0.0
8	7.181586412438508E-4	0.0	0.0	4.946317641567022	0.0
9	7.181586412438508E-4	0.0	0.0	6.051204711120686	0.0
10	7.181586412438508E-4	0.0	0.0	7.120183848612158	0.0
11	7.181586412438508E-4	0.0	0.0	8.493841789651334	0.0
12	7.181586412438508E-4	0.0	0.0	9.037308341412619	0.0
13	7.181586412438508E-4	0.0	0.0	9.278071025889618	0.0
14	7.181586412438508E-4	0.0	0.0	9.517397393084133	0.0
15	7.181586412438508E-4	0.0	0.0	9.717763653991167	0.0
16	7.181586412438508E-4	0.0	0.0	10.115982620560882	0.0
17	7.181586412438508E-4	0.0	0.0	10.616180114187223	0.0
18	7.181586412438508E-4	0.0	0.0	11.268088620776329	0.0
19	7.181586412438508E-4	0.0	0.0	11.560558727422888	0.0
20	8.976983015548135E-4	0.0	0.0	11.837408883622393	0.0
21	8.976983015548135E-4	0.0	0.0	12.180329634816331	0.0
22	0.0010772379618657763	0.0	0.0	12.5270207188768	0.0
23	0.0010772379618657763	0.0	0.0	12.901540450285468	0.0
24	0.0010772379618657763	0.0	0.0	13.168515925167869	0.0
25	0.0010772379618657763	0.0	0.0	13.383424898560092	0.0
26	0.0010772379618657763	0.0	0.0	13.587920571654278	0.0
27	0.0010772379618657763	0.0	0.0	13.812883766023914	0.0
28	0.0010772379618657763	0.0	0.0	14.027972279076447	0.0
29	0.0012567776221767389	0.0	0.0	14.264426011705986	0.0
30	0.0012567776221767389	0.0	0.0	14.559589213257208	0.0
31	0.0014363172824877015	0.0	0.0	14.829257783044275	0.0
32	0.0014363172824877015	0.0	0.0	15.052425580810802	0.0
33	0.0014363172824877015	0.0	0.0	15.29839491543682	0.0
34	0.0014363172824877015	0.0	0.0	15.538439441272578	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	60	0.0	40.000004	24
GTTTCGT	30	4.86299E-6	40.000004	36
CGTTGAT	60	0.0	40.000004	25
ACTTACG	30	4.86299E-6	40.000004	1
ATCGTTG	60	0.0	40.000004	23
TTGAGCG	30	4.86299E-6	40.000004	1
AACGGCA	30	4.86299E-6	40.000004	22
TCAGCTT	30	4.86299E-6	40.000004	13
GTTCGTA	30	4.86299E-6	40.000004	30
CGAGGAT	30	4.86299E-6	40.000004	12
ACAGCCG	30	4.86299E-6	40.000004	1
GACGGTC	30	4.86299E-6	40.000004	9
GGCATGT	30	4.86299E-6	40.000004	9
CGCGTTA	30	4.86299E-6	40.000004	30
CATCCGG	60	0.0	40.000004	2
TTATTCG	30	4.86299E-6	40.000004	1
TATCGCG	30	4.86299E-6	40.000004	1
TCACGGC	20	0.0012552117	40.0	15
GCGAGAC	55	5.456968E-12	40.0	21
CTTCGCG	20	0.0012552117	40.0	1
>>END_MODULE