##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636569
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208158110118465	31.0	31.0	34.0	30.0	34.0
2	31.399867100031575	31.0	31.0	34.0	30.0	34.0
3	31.58328005290864	31.0	31.0	34.0	30.0	34.0
4	35.52521721918598	37.0	35.0	37.0	33.0	37.0
5	35.117252018241544	37.0	35.0	37.0	32.0	37.0
6	35.13580774432936	37.0	35.0	37.0	32.0	37.0
7	34.30773568929684	37.0	35.0	37.0	32.0	37.0
8	34.64257606009718	37.0	35.0	37.0	32.0	37.0
9	35.78688563219384	39.0	35.0	39.0	31.0	39.0
10	35.50306879537018	37.0	35.0	39.0	30.0	39.0
11	35.95769351005154	37.0	35.0	39.0	31.0	39.0
12	35.73746129641877	37.0	35.0	39.0	31.0	39.0
13	35.47972018744237	37.0	35.0	39.0	30.0	39.0
14	36.54118563737788	38.0	35.0	40.0	31.0	41.0
15	36.67649539955606	38.0	35.0	40.0	31.0	41.0
16	36.675749211790084	38.0	35.0	40.0	31.0	41.0
17	36.62581746833415	38.0	35.0	40.0	31.0	41.0
18	36.42584071797401	38.0	35.0	40.0	31.0	41.0
19	36.3918915310045	38.0	35.0	40.0	31.0	41.0
20	36.33349094913513	38.0	35.0	40.0	30.0	41.0
21	36.32428849032862	38.0	35.0	40.0	30.0	41.0
22	36.294774015071425	38.0	35.0	40.0	30.0	41.0
23	36.18187030785351	38.0	35.0	40.0	30.0	41.0
24	35.800461536769774	38.0	34.0	40.0	29.0	41.0
25	35.872095562303535	38.0	34.0	40.0	30.0	41.0
26	35.756419178439415	38.0	34.0	40.0	30.0	41.0
27	35.58601816927937	38.0	34.0	40.0	29.0	41.0
28	35.48948346526457	38.0	34.0	40.0	29.0	41.0
29	35.30843474941444	37.0	34.0	40.0	27.0	41.0
30	35.267438408090875	37.0	34.0	40.0	27.0	41.0
31	35.13362573420949	37.0	34.0	40.0	27.0	41.0
32	35.065309495121504	37.0	34.0	40.0	27.0	41.0
33	35.029724978753286	37.0	34.0	40.0	27.0	41.0
34	34.94014945748222	37.0	34.0	40.0	26.0	41.0
35	34.80608072337798	37.0	33.0	40.0	25.0	41.0
36	34.655025613876894	37.0	33.0	40.0	25.0	41.0
37	34.477568024833126	37.0	33.0	40.0	24.0	41.0
38	34.43581607021391	37.0	33.0	40.0	24.0	41.0
39	34.34650446377376	37.0	33.0	40.0	24.0	41.0
40	34.12056195007926	36.0	33.0	40.0	23.0	41.0
41	34.068740387923384	36.0	33.0	40.0	23.0	41.0
42	34.01021570324662	36.0	33.0	40.0	23.0	41.0
43	33.96651894767103	36.0	33.0	40.0	23.0	41.0
44	33.84544644806769	36.0	33.0	40.0	23.0	41.0
45	33.68003311502759	35.0	33.0	39.0	23.0	41.0
46	33.55149559592126	35.0	33.0	39.0	23.0	41.0
47	33.39971314971354	35.0	32.0	39.0	22.0	40.0
48	33.28475938979121	35.0	32.0	39.0	22.0	40.0
49	33.10437046101836	35.0	32.0	39.0	20.0	40.0
50	32.934754912664616	35.0	32.0	39.0	20.0	40.0
51	31.016359577673434	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	15.0
11	12.0
12	29.0
13	28.0
14	54.0
15	84.0
16	207.0
17	402.0
18	770.0
19	1387.0
20	2158.0
21	3112.0
22	4341.0
23	5421.0
24	6398.0
25	7433.0
26	8758.0
27	10471.0
28	12525.0
29	15543.0
30	19699.0
31	24795.0
32	31987.0
33	43862.0
34	66327.0
35	67907.0
36	65118.0
37	84711.0
38	99329.0
39	53681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.759729110277124	35.52120822723067	19.045068170143377	24.67399449234883
2	22.63211058031415	33.27384776827021	19.85095095739818	24.243090694017457
3	20.01998212291205	35.419883783219106	20.787848607142354	23.772285486726496
4	19.34495710598537	35.65976351346044	18.888447285368905	26.106832095185283
5	16.98464738308023	38.19774447074866	16.837609120142513	27.9799990260286
6	18.100158820175032	40.735725428036865	18.510797729704084	22.653318022084015
7	80.30944013924649	13.07305256774992	3.289981133231433	3.327526159772153
8	83.36221210897797	10.291107484027656	2.7641936695000857	3.582486737494286
9	77.43449649605935	13.9433431411206	5.031190648617825	3.590969714202231
10	40.578319082456105	37.13972876467437	9.969854014254542	12.312098138614981
11	34.2242553438826	29.57809758250873	20.63594048720563	15.561706586403046
12	33.47571119548706	27.08661590495296	23.539474903741777	15.898197995818206
13	23.757047547084447	35.96844961033289	21.016888978256873	19.257613864325783
14	18.067483650633317	38.25602566257547	24.904134508592154	18.772356178199065
15	17.439429189922855	32.83870248158487	30.436449151623783	19.285419176868494
16	19.797539622570373	29.797398239625238	31.440739338547747	18.96432279925664
17	19.63290703757173	28.4544173530285	24.367507685733987	27.545167923665776
18	22.831617625112123	30.61663386058699	24.903349047785863	21.64839946651502
19	23.41395826689644	32.854411697710695	23.243199087608726	20.488430947784135
20	25.938900574800215	30.69235228231347	22.698089288042617	20.670657854843704
21	23.07825231828757	31.846351298916538	23.754219888181797	21.321176494614093
22	21.1364361129744	30.28092791197812	23.951841827044674	24.630794148002806
23	22.263101093518532	32.52090503935944	22.6044623599327	22.611531507189323
24	19.967356248890535	30.520179273574428	28.62093504396224	20.891529433572796
25	20.007728934333905	30.828865370446877	24.818205096383895	24.34520059883532
26	21.028356706028724	34.16943017960347	22.760140691739622	22.042072422628184
27	19.35579646511219	34.18215464466539	24.811764317772308	21.65028457245012
28	19.427901767129722	34.365009920369985	27.318326842808872	18.888761469691424
29	18.39941938737199	32.4258642817982	25.87245059058798	23.30226574024183
30	20.95530885104364	32.35344479545815	26.200930299778975	20.490316053719233
31	24.95676038261367	31.90054809455063	23.098517207089884	20.04417431574582
32	24.77563312068291	32.29170757608366	23.598698648536136	19.33396065469729
33	24.50983318383396	30.58662925778667	23.174549813138874	21.7289877452405
34	21.791039148937507	31.291973061836188	24.002896779453604	22.9140910097727
35	22.410610632940024	31.59861696061228	25.468409551831773	20.522362854615917
36	25.962307306827697	30.324285348485397	24.386044560762464	19.327362783924446
37	22.10129616742254	33.43800907678508	23.896388294120513	20.56430646167187
38	23.480565343269934	31.515358115145414	23.994413802745658	21.009662738838994
39	24.54313672202071	29.15190654901511	24.53496792963528	21.769988799328903
40	24.761809010492186	28.691312332205936	24.501193114964757	22.045685542337125
41	21.294627919361453	27.806883464322013	27.103267674046332	23.795220942270202
42	24.889996214078913	28.602712353256283	22.80554032634326	23.701751106321545
43	23.570264967348393	28.734983953035727	24.537010127731637	23.157740951884243
44	21.73071575901434	30.959408956452485	25.16333657466826	22.146538709864917
45	23.347508282684203	32.41706712076774	23.011645241914074	21.223779354633983
46	22.0995681536487	30.447602695073115	26.48793767839779	20.964891472880396
47	23.41207316096134	30.62417428432739	24.7248923525965	21.238860202114775
48	24.518944529186935	27.878517489855774	26.337286295751127	21.26525168520616
49	22.665571210662158	28.82405520846915	26.07839841399754	22.431975166871148
50	20.920277299083054	30.324599532807913	25.950525394733326	22.804597773375708
51	22.959019367892562	29.416921025057768	24.6658256999634	22.95823390708627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16985.0
1	13020.0
2	9055.0
3	5835.0
4	2615.0
5	2233.0
6	1851.0
7	1907.5
8	1964.0
9	1974.5
10	1985.0
11	2257.5
12	2530.0
13	2481.5
14	2433.0
15	2607.0
16	2781.0
17	2627.5
18	2474.0
19	2777.5
20	3081.0
21	2667.5
22	2254.0
23	2795.5
24	3337.0
25	3391.5
26	4466.0
27	5486.0
28	7543.5
29	9601.0
30	9866.0
31	10131.0
32	11204.0
33	12277.0
34	13925.5
35	15574.0
36	17062.0
37	18550.0
38	19874.0
39	21198.0
40	23243.0
41	25288.0
42	29596.0
43	33904.0
44	40913.0
45	47922.0
46	50177.5
47	52433.0
48	49198.0
49	45963.0
50	43499.5
51	41036.0
52	39526.5
53	38017.0
54	35700.5
55	33384.0
56	32199.5
57	31015.0
58	28866.5
59	26718.0
60	26226.5
61	25735.0
62	24609.0
63	23483.0
64	20425.5
65	17368.0
66	14960.5
67	12553.0
68	11427.5
69	10302.0
70	9051.0
71	7800.0
72	6562.0
73	5324.0
74	4334.5
75	2849.5
76	2354.0
77	1742.0
78	1130.0
79	1071.5
80	1013.0
81	743.5
82	474.0
83	322.0
84	170.0
85	151.5
86	133.0
87	90.0
88	47.0
89	36.0
90	25.0
91	20.0
92	15.0
93	10.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	636569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.562318693512776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84918324548666	19.858434049858566
2	8.060939935875593	3.9599075134848145
3	2.842796348830112	2.0947700970215917
4	1.3785325662267804	1.3543982488419275
5	0.7862120281092267	0.9655595197545928
6	0.5352604783859025	0.7888343072493987
7	0.3811805217008757	0.655387421764346
8	0.28196527667346855	0.5540576788926591
9	0.23279226472732187	0.5146126016055365
>10	2.7782780197918395	16.17634840826833
>50	1.0786100762363664	19.205617442354484
>100	0.7877122071908009	28.95413856794376
>500	0.0013074061530137778	0.22495105077824845
>1k	0.004575921535548221	2.3129335378020475
>5k	0.0	0.0
>10k+	6.537030765068889E-4	2.3800495543797124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14823	2.3285771063309713	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3424	0.5378835601482321	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2742	0.43074670617010885	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT	1849	0.2904634061664957	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	1717	0.26972724088040734	No Hit
AATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	1582	0.24851979911054417	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1551	0.24364994211153856	No Hit
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	1540	0.24192192833769788	TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	883	0.13871237839103068	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1418432251649074E-4	0.0	0.0	0.34277509586549143	0.0
2	3.1418432251649074E-4	0.0	0.0	0.42194954513964705	0.0
3	3.1418432251649074E-4	0.0	0.0	0.5506080252101501	0.0
4	3.1418432251649074E-4	0.0	0.0	1.3299422372123053	0.0
5	3.1418432251649074E-4	0.0	0.0	1.4600145467341326	0.0
6	3.1418432251649074E-4	0.0	0.0	1.9630236470830342	0.0
7	3.1418432251649074E-4	0.0	0.0	2.3463285205531528	0.0
8	3.1418432251649074E-4	0.0	0.0	2.534682021901789	0.0
9	3.1418432251649074E-4	0.0	0.0	3.194154914863903	0.0
10	3.1418432251649074E-4	0.0	0.0	3.705018623275717	0.0
11	3.1418432251649074E-4	0.0	0.0	4.5390209073957415	0.0
12	3.1418432251649074E-4	0.0	0.0	4.816131479855287	0.0
13	3.1418432251649074E-4	0.0	0.0	4.965211940889361	0.0
14	3.1418432251649074E-4	0.0	0.0	5.127488143469129	0.0
15	3.1418432251649074E-4	0.0	0.0	5.250648397895594	0.0
16	3.1418432251649074E-4	0.0	0.0	5.477018202268725	0.0
17	3.1418432251649074E-4	0.0	0.0	5.759941184694825	0.0
18	4.712764837747361E-4	0.0	0.0	6.125337551781504	0.0
19	4.712764837747361E-4	0.0	0.0	6.272847091202996	0.0
20	4.712764837747361E-4	0.0	0.0	6.426797409236077	0.0
21	4.712764837747361E-4	0.0	0.0	6.605725380909218	0.0
22	4.712764837747361E-4	0.0	0.0	6.795492711709178	0.0
23	4.712764837747361E-4	0.0	0.0	6.995471032990925	0.0
24	4.712764837747361E-4	0.0	0.0	7.1352830565107634	0.0
25	4.712764837747361E-4	0.0	0.0	7.2469755831653755	0.0
26	4.712764837747361E-4	0.0	0.0	7.357882649013697	0.0
27	4.712764837747361E-4	0.0	0.0	7.487797866374266	0.0
28	4.712764837747361E-4	0.0	0.0	7.601846775447752	0.0
29	4.712764837747361E-4	0.0	0.0	7.7173095139725625	0.0
30	4.712764837747361E-4	0.0	0.0	7.880999546003654	0.0
31	4.712764837747361E-4	0.0	0.0	8.019554832233426	0.0
32	4.712764837747361E-4	0.0	0.0	8.13925905911221	0.0
33	4.712764837747361E-4	0.0	0.0	8.270116829440328	0.0
34	4.712764837747361E-4	0.0	0.0	8.395790558446924	0.0
35	4.712764837747361E-4	0.0	0.0	8.553353996188944	0.0
36	4.712764837747361E-4	0.0	0.0	8.683897582194547	0.0
37	4.712764837747361E-4	0.0	0.0	8.813027338748824	0.0
38	4.712764837747361E-4	0.0	0.0	8.95205390146237	0.0
39	4.712764837747361E-4	0.0	0.0	9.086053515015655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGT	20	7.0309115E-4	45.0	31
CTAGACG	20	7.0309115E-4	45.0	1
GTACCTC	45	3.8380676E-10	45.0	22
ATCTCGC	20	7.0309115E-4	45.0	11
CGAACCT	20	7.0309115E-4	45.0	44
CGAACCC	35	1.2107921E-7	45.0	23
ACTGCGC	20	7.0309115E-4	45.0	27
CTCCGTT	25	3.8888382E-5	45.0	44
GTATTCG	45	3.8380676E-10	45.0	31
CGAGTGA	20	7.0309115E-4	45.0	26
TTTACGC	20	7.0309115E-4	45.0	33
GCGAATC	40	6.8066583E-9	45.0	37
CGTGCAA	20	7.0309115E-4	45.0	26
GACGTGA	20	7.0309115E-4	45.0	4
ATTACGC	25	3.8888382E-5	45.0	18
ACTCTCG	20	7.0309115E-4	45.0	27
TCCTCGA	20	7.0309115E-4	45.0	18
CACGAAT	20	7.0309115E-4	45.0	35
TAACGAT	20	7.0309115E-4	45.0	29
ATCACGT	20	7.0309115E-4	45.0	44
>>END_MODULE