SRR3548868_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150029
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15869	1.3798782465485653	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	4057	0.3527737126628981	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3603	0.313296447306981	No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	3478	0.3024271561847571	TruSeq Adapter, Index 12 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	3162	0.2749495882277751	RNA PCR Primer, Index 29 (96% over 26bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3142	0.2732105016482193	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3102	0.26973232848910766	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2338	0.20329922115007534	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1923	0.16721317462429208	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTA	25	3.8908074E-5	45.0	41
CGCATTG	20	7.03329E-4	45.0	29
ATAATCG	20	7.03329E-4	45.0	24
CGCATAC	20	7.03329E-4	45.0	16
ATTACGT	20	7.03329E-4	45.0	36
CGTCTCA	25	3.8908074E-5	45.0	20
CGTTACA	20	7.03329E-4	45.0	45
GTAGCTA	35	1.211938E-7	45.0	12
TCAATCG	25	3.8908074E-5	45.0	1
ACTAGAC	45	3.8562575E-10	45.0	31
CCGTACT	40	6.8157533E-9	45.0	44
GTATCGC	20	7.03329E-4	45.0	15
CGTACCG	55	1.8189894E-12	45.0	1
TACTCGC	160	0.0	45.0	45
CACGTAG	25	3.8908074E-5	45.0	1
TAGTGCG	40	6.8157533E-9	45.0	1
GATTCGC	20	7.03329E-4	45.0	9
TATCGAT	25	3.8908074E-5	45.0	13
TATCGAG	20	7.03329E-4	45.0	1
GTGCGAT	35	1.211938E-7	45.0	35