##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150029
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162528944922258	31.0	31.0	34.0	28.0	34.0
2	31.338137560009358	31.0	31.0	34.0	28.0	34.0
3	31.521656410403565	31.0	31.0	34.0	30.0	34.0
4	35.46548565297049	37.0	35.0	37.0	33.0	37.0
5	35.05058220270967	37.0	35.0	37.0	32.0	37.0
6	35.03500085650014	36.0	35.0	37.0	32.0	37.0
7	34.27258616956616	36.0	35.0	37.0	31.0	37.0
8	34.61438537636877	37.0	35.0	37.0	32.0	37.0
9	35.77351005931155	39.0	35.0	39.0	31.0	39.0
10	35.38931453032924	37.0	35.0	39.0	30.0	39.0
11	35.813258622173876	37.0	35.0	39.0	30.0	39.0
12	35.74187172671298	37.0	35.0	39.0	31.0	39.0
13	35.62239039189446	37.0	35.0	39.0	30.0	39.0
14	36.71106728612931	38.0	35.0	40.0	31.0	41.0
15	36.74682116711839	38.0	35.0	40.0	31.0	41.0
16	36.68807743109087	38.0	35.0	40.0	31.0	41.0
17	36.635909181420644	38.0	35.0	40.0	31.0	41.0
18	36.45511461015331	38.0	35.0	40.0	31.0	41.0
19	36.42635968310364	38.0	35.0	40.0	30.0	41.0
20	36.38821977532741	38.0	35.0	40.0	30.0	41.0
21	36.41065486174696	38.0	35.0	40.0	30.0	41.0
22	36.37676267294129	38.0	35.0	40.0	30.0	41.0
23	36.27497741361305	38.0	35.0	40.0	30.0	41.0
24	35.92020722955682	38.0	34.0	40.0	29.0	41.0
25	35.968682528875355	38.0	34.0	40.0	30.0	41.0
26	35.83481720895734	38.0	34.0	40.0	29.0	41.0
27	35.728139029537516	38.0	34.0	40.0	29.0	41.0
28	35.61572882075148	38.0	34.0	40.0	29.0	41.0
29	35.29756814828148	38.0	34.0	40.0	27.0	41.0
30	35.38483116512714	38.0	34.0	40.0	27.0	41.0
31	35.2244786870592	38.0	34.0	40.0	27.0	41.0
32	35.1360104832139	38.0	34.0	40.0	27.0	41.0
33	35.10330609054206	38.0	34.0	40.0	26.0	41.0
34	34.995022734209314	37.0	34.0	40.0	26.0	41.0
35	34.88479333999403	37.0	33.0	40.0	25.0	41.0
36	34.70410398346476	37.0	33.0	40.0	25.0	41.0
37	34.61403929813944	37.0	33.0	40.0	25.0	41.0
38	34.45594502399505	37.0	33.0	40.0	24.0	41.0
39	34.29247262460338	37.0	33.0	40.0	24.0	41.0
40	34.12733157163863	37.0	33.0	40.0	23.0	41.0
41	34.09240723494799	37.0	33.0	40.0	23.0	41.0
42	34.00842761356453	36.0	33.0	40.0	23.0	41.0
43	33.96338092343758	36.0	33.0	40.0	23.0	41.0
44	33.89744693394688	36.0	33.0	40.0	23.0	41.0
45	33.67000571289941	36.0	33.0	39.0	23.0	41.0
46	33.47758273921788	35.0	32.0	39.0	22.0	40.0
47	33.33699237149672	35.0	32.0	39.0	22.0	40.0
48	33.18870480657444	35.0	32.0	39.0	21.0	40.0
49	33.01281185083159	35.0	32.0	39.0	20.0	40.0
50	32.88163863693872	35.0	31.0	39.0	20.0	40.0
51	30.888309773057898	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	17.0
11	13.0
12	34.0
13	62.0
14	89.0
15	172.0
16	356.0
17	749.0
18	1405.0
19	2559.0
20	3964.0
21	5683.0
22	7799.0
23	9713.0
24	11897.0
25	14084.0
26	16248.0
27	19248.0
28	23559.0
29	28791.0
30	35608.0
31	44547.0
32	56898.0
33	75611.0
34	110377.0
35	117342.0
36	125301.0
37	159741.0
38	181470.0
39	96685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.598002311246063	33.195249858916604	19.988713328098683	26.21803450173865
2	24.049132674045612	33.11786050612637	19.13021323810095	23.702793581727068
3	20.442527971033776	32.700218864046036	20.297749013285753	26.55950415163444
4	21.16616189678695	33.83558153750905	18.057283772843988	26.94097279286001
5	17.825898303434087	37.26384291178744	16.625493791895682	28.28476499288279
6	19.065084445696588	38.94432227361223	19.01969428597018	22.970898994721004
7	81.39507786325389	11.285106723395671	3.802947577843689	3.5168678355067566
8	84.03205484383437	8.701258837820612	3.2547005336387174	4.011985784706298
9	78.93357471855057	12.047522279872942	5.321778842098764	3.6971241594777173
10	39.60030573142069	34.67660380738225	10.990157639503003	14.732932821694062
11	30.258280443362732	28.983182163232406	21.87075282449399	18.88778456891087
12	29.053267352388506	26.62358949209107	26.177513784435003	18.145629371085427
13	24.52590325983084	31.617289650956625	23.815834209398197	20.040972879814333
14	19.518116499670878	32.51352791973072	28.03711906395404	19.93123651664436
15	19.385858965295657	29.72290263984647	29.262218604922136	21.629019789935732
16	21.092772443129697	28.52441112354558	28.2945038777283	22.088312555596424
17	20.67217435386412	27.953121182161496	25.66804837095412	25.706656093020264
18	22.934117313563394	29.143091174222562	25.147365849035115	22.77542566317893
19	22.56517009571063	32.074408558392875	23.010637123063855	22.349784222832643
20	25.534486521644233	30.300279384259003	23.780530751833215	20.384703342263542
21	23.93191823858355	29.616731404164593	24.517468689919994	21.933881667331868
22	23.78505237694006	27.830515578302805	24.072436434211657	24.311995610545473
23	22.17474515860035	31.396947381326907	23.427843993499295	23.00046346657345
24	21.521718147977136	29.925071454719838	27.294181277167795	21.25902912013523
25	20.98086222173528	29.47021335983701	24.354777140402547	25.194147278025163
26	22.811859527020623	31.11460667513602	22.319698025006325	23.753835772837036
27	20.460527517132178	30.601923951482963	24.15026055864678	24.787287972738078
28	20.33835668491838	31.356165801036322	26.05908198836725	22.246395525678047
29	20.574785505408993	32.20701391008401	24.375472270699262	22.84272831380774
30	22.680036764290293	29.199698442387106	24.775549138326078	23.344715654996527
31	25.34579562776243	30.50131779285566	21.257724805200564	22.895161774181346
32	24.539468135151374	29.673512580987087	23.065244441661907	22.721774842199633
33	23.151155318691963	28.907966668666617	22.616125332491617	25.324752680149803
34	20.930167847941224	31.043478034032184	23.655403472434173	24.370950645592416
35	21.787885348978158	29.3023045505809	24.60972723296543	24.300082867475517
36	24.097740143944197	30.44810174352125	23.746531609202897	21.707626503331657
37	21.42485102549588	30.928959182768434	23.631751894952213	24.014437896783473
38	22.292220457049343	30.456536313432096	23.732097190592587	23.519146038925975
39	22.231439381093868	29.772379653034836	23.77679171568717	24.219389250184125
40	24.69876846583869	28.55249737180541	22.334654169590507	24.4140799927654
41	20.784171529587514	26.080646661953743	27.082273577448916	26.052908231009823
42	23.591492040635497	26.577416743403866	23.26706543921936	26.564025776741286
43	22.690123466451713	27.094186320518876	24.933023428104857	25.282666784924558
44	22.48760683426244	27.074447687840912	24.59355372777556	25.844391750121083
45	23.242283455460687	28.773361367408995	24.54503321220595	23.43932196492436
46	22.975420619827847	27.86199304539277	26.51211404234154	22.650472292437843
47	22.364044732785	28.82927300094171	24.556337274973068	24.250344991300217
48	22.755947893487903	27.12548987895088	26.580547099247063	23.538015128314154
49	23.092461146631955	26.75497748317651	26.933407766238936	23.2191536039526
50	21.732756304406237	27.47626364204729	26.2233387158063	24.567641337740177
51	21.93501207360858	26.85697491106746	26.15629692816442	25.05171608715954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18556.0
1	14496.5
2	10437.0
3	7069.0
4	3701.0
5	3319.0
6	2937.0
7	3019.5
8	3102.0
9	3381.0
10	3660.0
11	3879.5
12	4099.0
13	4102.0
14	4105.0
15	4121.0
16	4137.0
17	4104.0
18	4071.0
19	4405.0
20	4739.0
21	4442.0
22	4145.0
23	4715.5
24	5286.0
25	6807.0
26	8746.0
27	9164.0
28	10360.5
29	11557.0
30	13101.0
31	14645.0
32	19539.5
33	24434.0
34	26022.5
35	27611.0
36	29941.0
37	32271.0
38	33792.0
39	35313.0
40	39578.5
41	43844.0
42	52342.0
43	60840.0
44	69100.0
45	77360.0
46	81169.0
47	84978.0
48	84850.5
49	84723.0
50	83444.5
51	82166.0
52	76593.5
53	71021.0
54	66966.0
55	62911.0
56	60632.5
57	58354.0
58	54061.5
59	49769.0
60	48214.0
61	46659.0
62	45847.0
63	45035.0
64	41042.0
65	37049.0
66	33879.5
67	30710.0
68	27105.0
69	23500.0
70	21035.5
71	18571.0
72	16992.0
73	15413.0
74	12136.5
75	7193.0
76	5526.0
77	4170.0
78	2814.0
79	2206.0
80	1598.0
81	1238.5
82	879.0
83	681.5
84	484.0
85	430.0
86	376.0
87	264.5
88	153.0
89	107.5
90	62.0
91	51.5
92	41.0
93	31.0
94	21.0
95	15.5
96	10.0
97	7.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1150029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.95736925971347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01450042246759	19.969514331750233
2	8.591307005471254	4.288328427182185
3	3.2425825561638395	2.427789906278596
4	1.6167423536480294	1.6139854367124853
5	0.8804527686346347	1.0986892431275823
6	0.5559539833842947	0.8325089312838269
7	0.3996821974358183	0.6982511331557595
8	0.27059208353885605	0.5402613238107572
9	0.2248328779018848	0.5050113439969269
>10	2.3877619848997855	14.145505512130546
>50	0.961757637595332	17.5230296403242
>100	0.8460494828434079	31.921967784205503
>500	0.004600018099943245	0.843193797593158
>1k	0.002830780369195843	2.1905553151757737
>5k	0.0	0.0
>10k+	3.5384754614948037E-4	1.4014078732724997
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15869	1.3798782465485653	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	4057	0.3527737126628981	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3603	0.313296447306981	No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	3478	0.3024271561847571	TruSeq Adapter, Index 12 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	3162	0.2749495882277751	RNA PCR Primer, Index 29 (96% over 26bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	3142	0.2732105016482193	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3102	0.26973232848910766	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2338	0.20329922115007534	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1923	0.16721317462429208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.73908657955582E-4	0.0	0.0	0.3996420959819274	0.0
2	1.73908657955582E-4	0.0	0.0	0.47442281890282767	0.0
3	1.73908657955582E-4	0.0	0.0	0.6323318803264961	0.0
4	1.73908657955582E-4	0.0	0.0	1.550569594331969	0.0
5	1.73908657955582E-4	0.0	0.0	1.696131141040791	0.0
6	1.73908657955582E-4	0.0	0.0	2.3018549967000834	0.0
7	1.73908657955582E-4	0.0	0.0	2.7950599506621137	0.0
8	1.73908657955582E-4	0.0	0.0	3.0727051230882005	0.0
9	1.73908657955582E-4	0.0	0.0	3.928683537545575	0.0
10	1.73908657955582E-4	0.0	0.0	4.552406939303269	0.0
11	1.73908657955582E-4	0.0	0.0	5.455079828421718	0.0
12	1.73908657955582E-4	0.0	0.0	5.780897699101501	0.0
13	1.73908657955582E-4	0.0	0.0	5.932719957496724	0.0
14	1.73908657955582E-4	0.0	0.0	6.106802524110262	0.0
15	1.73908657955582E-4	0.0	0.0	6.222364827321746	0.0
16	1.73908657955582E-4	0.0	0.0	6.458619739154404	0.0
17	1.73908657955582E-4	0.0	0.0	6.75165582780956	0.0
18	1.73908657955582E-4	0.0	0.0	7.147993659290331	0.0
19	1.73908657955582E-4	0.0	0.0	7.298076831105998	0.0
20	1.73908657955582E-4	0.0	0.0	7.475289753562736	0.0
21	1.73908657955582E-4	0.0	0.0	7.66685014030081	0.0
22	1.73908657955582E-4	0.0	0.0	7.868323320542352	0.0
23	1.73908657955582E-4	0.0	0.0	8.081970106840783	0.0
24	1.73908657955582E-4	0.0	0.0	8.23840094467183	0.0
25	1.73908657955582E-4	0.0	0.0	8.375440967140829	0.0
26	1.73908657955582E-4	0.0	0.0	8.503785556712048	0.0
27	1.73908657955582E-4	0.0	0.0	8.638216949311714	0.0
28	1.73908657955582E-4	0.0	0.0	8.781343774809157	0.0
29	1.73908657955582E-4	0.0	0.0	8.927514001820823	0.0
30	1.73908657955582E-4	0.0	0.0	9.119248297216853	0.0
31	1.73908657955582E-4	0.0	0.0	9.264722889596698	0.0
32	1.73908657955582E-4	0.0	0.0	9.407588852107208	0.0
33	1.73908657955582E-4	0.0	0.0	9.566802228465543	0.0
34	1.73908657955582E-4	0.0	0.0	9.71845057820281	0.0
35	1.73908657955582E-4	0.0	0.0	9.89366355109306	0.0
36	1.73908657955582E-4	0.0	0.0	10.042268499316105	0.0
37	2.6086298693337297E-4	0.0	0.0	10.185221416155592	0.0
38	2.6086298693337297E-4	0.0	0.0	10.347739057015083	0.0
39	2.6086298693337297E-4	0.0	0.0	10.510169743545598	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTA	25	3.8908074E-5	45.0	41
CGCATTG	20	7.03329E-4	45.0	29
ATAATCG	20	7.03329E-4	45.0	24
CGCATAC	20	7.03329E-4	45.0	16
ATTACGT	20	7.03329E-4	45.0	36
CGTCTCA	25	3.8908074E-5	45.0	20
CGTTACA	20	7.03329E-4	45.0	45
GTAGCTA	35	1.211938E-7	45.0	12
TCAATCG	25	3.8908074E-5	45.0	1
ACTAGAC	45	3.8562575E-10	45.0	31
CCGTACT	40	6.8157533E-9	45.0	44
GTATCGC	20	7.03329E-4	45.0	15
CGTACCG	55	1.8189894E-12	45.0	1
TACTCGC	160	0.0	45.0	45
CACGTAG	25	3.8908074E-5	45.0	1
TAGTGCG	40	6.8157533E-9	45.0	1
GATTCGC	20	7.03329E-4	45.0	9
TATCGAT	25	3.8908074E-5	45.0	13
TATCGAG	20	7.03329E-4	45.0	1
GTGCGAT	35	1.211938E-7	45.0	35
>>END_MODULE