Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548867_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1238872 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 20011 | 1.615259687845072 | No Hit |
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT | 5473 | 0.4417728385176192 | RNA PCR Primer, Index 29 (95% over 21bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4762 | 0.3843819216190212 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT | 3991 | 0.32214788937033045 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3853 | 0.3110087240651173 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT | 3333 | 0.2690350576976476 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGT | 2676 | 0.21600294461413286 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT | 2505 | 0.20220006586636877 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2400 | 0.1937246140037066 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT | 1392 | 0.11236027612214981 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCGTAA | 25 | 7.795004E-5 | 40.000004 | 21 |
TTGGACG | 20 | 0.0012558063 | 40.000004 | 37 |
CCGATTC | 20 | 0.0012558063 | 40.000004 | 16 |
GCGCCTA | 20 | 0.0012558063 | 40.000004 | 35 |
CACGATC | 25 | 7.795004E-5 | 40.000004 | 22 |
TCGATAG | 25 | 7.795004E-5 | 40.000004 | 1 |
ATCGTAC | 25 | 7.795004E-5 | 40.000004 | 29 |
TACGCCT | 20 | 0.0012558063 | 40.000004 | 19 |
TCTACCG | 25 | 7.795004E-5 | 40.000004 | 1 |
TCGCTTA | 20 | 0.0012558063 | 40.000004 | 35 |
TTGACGT | 25 | 7.795004E-5 | 40.000004 | 12 |
TCGTCGA | 20 | 0.0012558063 | 40.000004 | 24 |
TCCAACG | 50 | 7.8216544E-11 | 40.000004 | 1 |
CGAGCAT | 50 | 7.8216544E-11 | 40.000004 | 10 |
CGTGTAA | 20 | 0.0012558063 | 40.000004 | 22 |
GACGATT | 25 | 7.795004E-5 | 40.000004 | 12 |
GCTAACG | 40 | 1.9284926E-8 | 40.000004 | 1 |
CAATCGA | 25 | 7.795004E-5 | 40.000004 | 33 |
TCGACGA | 25 | 7.795004E-5 | 40.000004 | 10 |
CGTTTAA | 20 | 0.0012558063 | 40.000004 | 27 |