FastQCFastQC Report
Sat 18 Jun 2016
SRR3548867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238872
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200111.615259687845072No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT54730.4417728385176192RNA PCR Primer, Index 29 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47620.3843819216190212No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT39910.32214788937033045No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38530.3110087240651173No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT33330.2690350576976476No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGT26760.21600294461413286No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT25050.20220006586636877No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24000.1937246140037066No Hit
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT13920.11236027612214981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA257.795004E-540.00000421
TTGGACG200.001255806340.00000437
CCGATTC200.001255806340.00000416
GCGCCTA200.001255806340.00000435
CACGATC257.795004E-540.00000422
TCGATAG257.795004E-540.0000041
ATCGTAC257.795004E-540.00000429
TACGCCT200.001255806340.00000419
TCTACCG257.795004E-540.0000041
TCGCTTA200.001255806340.00000435
TTGACGT257.795004E-540.00000412
TCGTCGA200.001255806340.00000424
TCCAACG507.8216544E-1140.0000041
CGAGCAT507.8216544E-1140.00000410
CGTGTAA200.001255806340.00000422
GACGATT257.795004E-540.00000412
GCTAACG401.9284926E-840.0000041
CAATCGA257.795004E-540.00000433
TCGACGA257.795004E-540.00000410
CGTTTAA200.001255806340.00000427