##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1238872
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046226728830742	31.0	31.0	34.0	28.0	34.0
2	31.35466375864496	31.0	31.0	34.0	28.0	34.0
3	31.498116028128813	31.0	31.0	34.0	28.0	34.0
4	35.46946012178821	37.0	35.0	37.0	33.0	37.0
5	35.04252093840203	37.0	35.0	37.0	32.0	37.0
6	35.19360999360709	37.0	35.0	37.0	32.0	37.0
7	35.16692281365629	37.0	35.0	37.0	32.0	37.0
8	35.55142016285782	37.0	35.0	37.0	35.0	37.0
9	36.911876287461496	39.0	37.0	39.0	33.0	39.0
10	36.1061546309869	37.0	35.0	39.0	31.0	39.0
11	36.3170464745349	38.0	35.0	39.0	32.0	39.0
12	36.148361574077065	37.0	35.0	39.0	32.0	39.0
13	35.90634060661634	38.0	35.0	39.0	31.0	39.0
14	37.085944310630964	39.0	36.0	40.0	32.0	41.0
15	36.98428086194538	39.0	35.0	40.0	32.0	41.0
16	36.87192865768215	38.0	35.0	40.0	31.0	41.0
17	36.856068262096485	38.0	35.0	40.0	32.0	41.0
18	36.68970240670546	38.0	35.0	40.0	31.0	41.0
19	36.90162744819481	39.0	35.0	40.0	31.0	41.0
20	36.70696084825551	38.0	35.0	40.0	31.0	41.0
21	36.818081286848034	39.0	35.0	40.0	31.0	41.0
22	36.89072801709943	39.0	35.0	40.0	31.0	41.0
23	36.70713035729276	38.0	35.0	40.0	31.0	41.0
24	36.322878392602306	38.0	35.0	40.0	30.0	41.0
25	36.505351642461854	38.0	35.0	40.0	31.0	41.0
26	36.520220813772525	38.0	35.0	40.0	31.0	41.0
27	36.42856808451559	38.0	35.0	40.0	31.0	41.0
28	36.399912178174986	38.0	35.0	40.0	31.0	41.0
29	36.04406266345514	38.0	35.0	40.0	30.0	41.0
30	36.12012782595781	38.0	35.0	40.0	30.0	41.0
31	36.03770365300047	38.0	35.0	40.0	30.0	41.0
32	36.00254344274469	38.0	35.0	40.0	30.0	41.0
33	35.99775521603523	38.0	35.0	40.0	30.0	41.0
34	35.922734552076406	38.0	35.0	40.0	30.0	41.0
35	35.705306117177564	38.0	34.0	40.0	29.0	41.0
36	35.674701664094435	38.0	34.0	40.0	29.0	41.0
37	35.59281023382561	38.0	34.0	40.0	29.0	41.0
38	35.383892766968664	38.0	34.0	40.0	28.0	41.0
39	35.29556241484189	38.0	34.0	40.0	27.0	41.0
40	35.131194344532766	37.0	34.0	40.0	27.0	41.0
41	35.18952240425161	37.0	34.0	40.0	27.0	41.0
42	35.07088787219342	37.0	34.0	40.0	27.0	41.0
43	35.04861277032656	37.0	34.0	40.0	27.0	41.0
44	34.97109709477654	37.0	34.0	40.0	27.0	41.0
45	34.750203410844705	37.0	34.0	40.0	26.0	41.0
46	33.6293475032126	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	5.0
10	14.0
11	19.0
12	15.0
13	34.0
14	61.0
15	78.0
16	151.0
17	300.0
18	553.0
19	1058.0
20	1934.0
21	3079.0
22	4578.0
23	6543.0
24	8474.0
25	10499.0
26	12574.0
27	14944.0
28	17909.0
29	22796.0
30	29093.0
31	38250.0
32	50296.0
33	70042.0
34	111248.0
35	126296.0
36	128279.0
37	177528.0
38	240438.0
39	161781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.368770946473887	32.408594269625915	19.941931046952387	27.280703736947803
2	24.496235285001195	32.307696033165655	18.518620164149326	24.67744851768383
3	20.354160881834442	32.51869442525136	20.11474954636153	27.01239514655267
4	20.483956373216927	33.917466856947286	18.21188952531012	27.386687244525664
5	18.027447549060756	36.76586443151512	16.697770229692818	28.508917789731303
6	19.191409604866365	38.47814786354038	19.1742972639627	23.156145267630553
7	82.73800683202138	9.403473482329087	4.135536197444126	3.7229834882053994
8	84.44318702819984	7.837371415287454	3.5547659483788476	4.164675608133851
9	80.02820307505537	10.23213051872994	5.917721927689059	3.821944478525627
10	39.30688561853041	34.46982416262536	11.152968183960892	15.070322034883347
11	29.681113141632064	28.801926268411908	22.2683215053694	19.248639084586625
12	28.953273623102305	26.50976049180222	26.217720636191633	18.319245248903844
13	24.507535887484742	31.433998024008936	24.04711705486927	20.01134903363705
14	19.372219244603155	32.17717407448066	28.161343544772986	20.2892631361432
15	19.222728417463628	29.31303637502502	29.46301151369956	22.001223693811788
16	20.89755842411484	28.73210468878141	28.13825802827088	22.232078858832875
17	20.383381011113336	27.750970237441802	26.013986917131067	25.851661834313795
18	22.693304877340033	28.69674994672573	25.354435325037617	23.255509850896622
19	22.499499544747156	32.092419555854036	23.30789621526679	22.100184684132017
20	25.11591189404555	30.229192362084216	24.012085187170264	20.642810556699967
21	23.578868519104475	29.822370672676435	24.939138183767167	21.65962262445192
22	23.508078316403953	27.882299382018484	24.299846957554937	24.309775344022626
23	21.93446942056968	31.462975997520324	23.52737005921516	23.07518452269484
24	21.1777326471177	29.794361322235062	27.337852498078895	21.69005353256834
25	20.667106852039595	29.635668575930364	24.58583291897791	25.111391653052127
26	22.657546542338515	31.098289411658346	22.663196843580288	23.580967202422848
27	20.175288488237687	30.67621190889777	24.049861486900987	25.098638115963556
28	20.09432774330197	31.263197489329002	26.432512801968244	22.209961965400783
29	20.38370388547001	32.37186731155438	24.330600740028025	22.913828062947584
30	22.381408248794063	29.41716335505202	24.854706539497222	23.346721856656703
31	25.007506828792643	30.46472920527706	21.473646995008362	23.054116970921935
32	24.430449634829102	29.736163219444784	22.998501862985037	22.834885282741073
33	22.67974415435977	28.8674697628165	22.926985192982002	25.525800889841726
34	20.672676434692203	30.911103003377267	23.817472668685706	24.598747893244823
35	21.32875712745142	29.71380417024519	24.643304554465676	24.31413414783771
36	23.47797028264421	30.532129227232513	23.98294577648054	22.00695471364273
37	21.007820016918615	30.87155089468484	23.77081732414648	24.34981176425006
38	22.030443823090682	30.549725879671186	23.840961778133657	23.578868519104475
39	21.995250518213343	29.71364273306685	23.933868874266267	24.357237874453535
40	24.415839570189657	28.40123919178091	22.51532038822413	24.667600849805307
41	20.48161553413105	26.118356052925563	26.96824207827766	26.43178633466573
42	23.491450287035303	26.611385195564996	23.370937433407164	26.526227083992538
43	22.199145674452243	27.094889544682584	25.267662841681787	25.43830193918339
44	22.374143575768926	26.92465404012683	24.639591499363938	26.06161088474031
45	22.935379926255496	28.50423611155955	25.083785895556606	23.476598066628352
46	22.552208783473997	27.994578939551463	26.67757443868293	22.77563783829161
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23506.0
1	18520.5
2	13535.0
3	9158.0
4	4781.0
5	4335.0
6	3889.0
7	3889.0
8	3942.0
9	3995.0
10	4056.0
11	4117.0
12	4290.0
13	4463.0
14	4381.0
15	4299.0
16	4454.5
17	4610.0
18	4792.0
19	4974.0
20	4974.0
21	4785.0
22	4596.0
23	5305.0
24	6014.0
25	7334.5
26	8655.0
27	10349.0
28	12043.0
29	12857.0
30	13671.0
31	13671.0
32	17787.0
33	21903.0
34	24264.5
35	26626.0
36	28317.0
37	30008.0
38	37236.0
39	44464.0
40	52473.5
41	60483.0
42	62950.0
43	65417.0
44	65417.0
45	79793.0
46	94169.0
47	99155.5
48	104142.0
49	105423.0
50	106704.0
51	98160.0
52	89616.0
53	84448.0
54	79280.0
55	75435.0
56	71590.0
57	71590.0
58	65307.0
59	59024.0
60	58850.0
61	58676.0
62	54694.5
63	50713.0
64	48973.0
65	47233.0
66	42210.0
67	37187.0
68	32117.5
69	27048.0
70	27048.0
71	23955.0
72	20862.0
73	16741.5
74	12621.0
75	9239.5
76	5858.0
77	4904.5
78	3951.0
79	3041.0
80	2131.0
81	2131.0
82	1640.5
83	1150.0
84	928.5
85	707.0
86	407.5
87	108.0
88	67.0
89	26.0
90	19.0
91	12.0
92	9.0
93	6.0
94	6.0
95	6.5
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1238872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.256548004524475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98981839502463	14.633015857788886
2	9.98627441413071	3.846023452841251
3	3.6445045584703553	2.10541730948678
4	1.8133189506320702	1.3967305368144163
5	1.079405472457302	1.039281164836023
6	0.6950475825944311	0.8030530283795011
7	0.5406342851108492	0.7287525044890187
8	0.3782431059665453	0.5826925221940175
9	0.31398699878830577	0.5441675143467221
>10	3.0938560958303043	13.817830084716705
>50	1.176760421758934	16.50183254120892
>100	1.2771795611287429	38.94451003957644
>500	0.006328937369319836	0.8673329772877131
>1k	0.0037973624215919015	2.118811183298265
>5k	4.219291579546557E-4	0.44467572690357404
>10k+	4.219291579546557E-4	1.6258735558317965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20011	1.615259687845072	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	5473	0.4417728385176192	RNA PCR Primer, Index 29 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4762	0.3843819216190212	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	3991	0.32214788937033045	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3853	0.3110087240651173	No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	3333	0.2690350576976476	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGT	2676	0.21600294461413286	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT	2505	0.20220006586636877	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2400	0.1937246140037066	No Hit
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	1392	0.11236027612214981	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.071858916821108E-5	0.0	0.0	0.5062669912630199	0.0
2	8.071858916821108E-5	0.0	0.0	0.6206452321143751	0.0
3	8.071858916821108E-5	0.0	0.0	0.8227645793915755	0.0
4	8.071858916821108E-5	0.0	0.0	1.7951007045118463	0.0
5	8.071858916821108E-5	0.0	0.0	1.9691299827585094	0.0
6	8.071858916821108E-5	0.0	0.0	2.727077535048011	0.0
7	8.071858916821108E-5	0.0	0.0	3.303166105941534	0.0
8	8.071858916821108E-5	0.0	0.0	3.6638974809342693	0.0
9	8.071858916821108E-5	0.0	0.0	4.494814637831834	0.0
10	8.071858916821108E-5	0.0	0.0	5.320485086433465	0.0
11	8.071858916821108E-5	0.0	0.0	6.305332592874809	0.0
12	8.071858916821108E-5	0.0	0.0	6.717400990578526	0.0
13	8.071858916821108E-5	0.0	0.0	6.885457093226742	8.071858916821108E-5
14	8.071858916821108E-5	0.0	0.0	7.081522546316326	8.071858916821108E-5
15	8.071858916821108E-5	0.0	0.0	7.22350654466321	8.071858916821108E-5
16	8.071858916821108E-5	0.0	0.0	7.516676460522152	8.071858916821108E-5
17	8.071858916821108E-5	0.0	0.0	7.873210468878141	8.071858916821108E-5
18	8.071858916821108E-5	0.0	0.0	8.367046797409255	8.071858916821108E-5
19	8.071858916821108E-5	0.0	0.0	8.557623386435402	8.071858916821108E-5
20	8.071858916821108E-5	0.0	0.0	8.76708812532691	8.071858916821108E-5
21	8.071858916821108E-5	0.0	0.0	9.00714520951317	8.071858916821108E-5
22	8.071858916821108E-5	0.0	0.0	9.253579062243718	8.071858916821108E-5
23	1.6143717833642216E-4	0.0	0.0	9.520434718033824	8.071858916821108E-5
24	1.6143717833642216E-4	0.0	0.0	9.7020515436623	8.071858916821108E-5
25	2.4215576750463325E-4	0.0	0.0	9.871560580915542	8.071858916821108E-5
26	2.4215576750463325E-4	0.0	0.0	10.031867699003609	8.071858916821108E-5
27	2.4215576750463325E-4	0.0	0.0	10.200488831776003	8.071858916821108E-5
28	2.4215576750463325E-4	0.0	0.0	10.373307331185142	8.071858916821108E-5
29	2.4215576750463325E-4	0.0	0.0	10.559121523450365	8.071858916821108E-5
30	2.4215576750463325E-4	0.0	0.0	10.804909627467568	8.071858916821108E-5
31	2.4215576750463325E-4	0.0	0.0	10.982006212102622	8.071858916821108E-5
32	2.4215576750463325E-4	0.0	0.0	11.14998159616167	8.071858916821108E-5
33	2.4215576750463325E-4	0.0	0.0	11.335069321124378	8.071858916821108E-5
34	2.4215576750463325E-4	0.0	0.0	11.522013573637954	8.071858916821108E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	25	7.795004E-5	40.000004	21
TTGGACG	20	0.0012558063	40.000004	37
CCGATTC	20	0.0012558063	40.000004	16
GCGCCTA	20	0.0012558063	40.000004	35
CACGATC	25	7.795004E-5	40.000004	22
TCGATAG	25	7.795004E-5	40.000004	1
ATCGTAC	25	7.795004E-5	40.000004	29
TACGCCT	20	0.0012558063	40.000004	19
TCTACCG	25	7.795004E-5	40.000004	1
TCGCTTA	20	0.0012558063	40.000004	35
TTGACGT	25	7.795004E-5	40.000004	12
TCGTCGA	20	0.0012558063	40.000004	24
TCCAACG	50	7.8216544E-11	40.000004	1
CGAGCAT	50	7.8216544E-11	40.000004	10
CGTGTAA	20	0.0012558063	40.000004	22
GACGATT	25	7.795004E-5	40.000004	12
GCTAACG	40	1.9284926E-8	40.000004	1
CAATCGA	25	7.795004E-5	40.000004	33
TCGACGA	25	7.795004E-5	40.000004	10
CGTTTAA	20	0.0012558063	40.000004	27
>>END_MODULE