FastQCFastQC Report
Sat 18 Jun 2016
SRR3548863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701100
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143662.049065753815433No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36600.5220367993153616No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28690.4092140921409214No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT26950.38439594922265014No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18170.2591641705890743No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT13070.1864213378975895No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT11520.16431322207958923No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT11340.16174582798459564No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT9410.13421765796605334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT257.791538E-540.0000049
TTTACGG507.8216544E-1140.0000042
GAATCGG257.791538E-540.0000042
CGCTGTT257.791538E-540.00000414
TAACGGA257.791538E-540.00000413
CGTTCTA257.791538E-540.00000413
TAGCCGT257.791538E-540.00000430
CGTTAGC257.791538E-540.00000431
TACGCAG257.791538E-540.0000041
CCTCCGT257.791538E-540.00000437
CGTGTCA257.791538E-540.00000437
CCGGTTA257.791538E-540.00000419
ACGTACG257.791538E-540.0000041
ATCATTC257.791538E-540.00000419
GATTACG257.791538E-540.0000041
CGATACC257.791538E-540.00000433
CCGTTGC257.791538E-540.00000433
ATACACG257.791538E-540.0000041
TGTCCAA257.791538E-540.00000413
TCACGCC353.0541014E-740.014