##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	701100
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.061193838254173	31.0	31.0	34.0	28.0	34.0
2	31.36113535872201	31.0	31.0	34.0	28.0	34.0
3	31.50382399087149	31.0	31.0	34.0	28.0	34.0
4	35.474987876194554	37.0	35.0	37.0	33.0	37.0
5	35.04387533875339	37.0	35.0	37.0	32.0	37.0
6	35.20389102838397	37.0	35.0	37.0	32.0	37.0
7	35.1205106261589	37.0	35.0	37.0	32.0	37.0
8	35.54714163457424	37.0	35.0	37.0	35.0	37.0
9	36.8740379403794	39.0	37.0	39.0	32.0	39.0
10	36.16271715875053	37.0	35.0	39.0	31.0	39.0
11	36.359041506204534	38.0	35.0	39.0	32.0	39.0
12	36.106779346740836	37.0	35.0	39.0	32.0	39.0
13	35.836123234916556	38.0	35.0	39.0	30.0	39.0
14	37.02747396947654	39.0	35.0	40.0	31.0	41.0
15	36.942453287690775	39.0	35.0	40.0	32.0	41.0
16	36.83349308229924	38.0	35.0	40.0	31.0	41.0
17	36.80559834545714	38.0	35.0	40.0	31.0	41.0
18	36.59786193125089	38.0	35.0	40.0	31.0	41.0
19	36.79692055341606	38.0	35.0	40.0	31.0	41.0
20	36.548456710882895	38.0	35.0	40.0	31.0	41.0
21	36.65582227927543	38.0	35.0	40.0	31.0	41.0
22	36.738582227927544	38.0	35.0	40.0	31.0	41.0
23	36.51931393524462	38.0	35.0	40.0	31.0	41.0
24	36.09522322065326	38.0	34.0	40.0	30.0	41.0
25	36.31133504492939	38.0	35.0	40.0	30.0	41.0
26	36.33226786478391	38.0	35.0	40.0	31.0	41.0
27	36.210275281700184	38.0	35.0	40.0	30.0	41.0
28	36.1929838824704	38.0	35.0	40.0	30.0	41.0
29	35.861889887319926	38.0	34.0	40.0	30.0	41.0
30	35.84290115532734	38.0	34.0	40.0	30.0	41.0
31	35.819293966623874	38.0	34.0	40.0	30.0	41.0
32	35.720011410640424	38.0	34.0	40.0	29.0	41.0
33	35.690912851233776	38.0	34.0	40.0	29.0	41.0
34	35.61331764370275	38.0	34.0	40.0	29.0	41.0
35	35.38069890172586	38.0	34.0	40.0	27.0	41.0
36	35.34427328483811	38.0	34.0	40.0	27.0	41.0
37	35.2174269005848	38.0	34.0	40.0	27.0	41.0
38	34.98735558408216	37.0	34.0	40.0	26.0	41.0
39	34.93124946512623	37.0	34.0	40.0	26.0	41.0
40	34.773574383112255	37.0	34.0	40.0	25.0	41.0
41	34.82836542575952	37.0	34.0	40.0	26.0	41.0
42	34.69347168734845	37.0	34.0	40.0	25.0	41.0
43	34.61108686350021	37.0	34.0	40.0	25.0	41.0
44	34.50442019683355	37.0	33.0	40.0	24.0	41.0
45	34.31798744829553	36.0	33.0	40.0	24.0	41.0
46	33.24084581372129	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	11.0
11	16.0
12	21.0
13	20.0
14	40.0
15	56.0
16	101.0
17	232.0
18	422.0
19	739.0
20	1278.0
21	2284.0
22	3176.0
23	4318.0
24	5733.0
25	7024.0
26	8287.0
27	9399.0
28	10945.0
29	13395.0
30	17267.0
31	21879.0
32	28849.0
33	40545.0
34	64786.0
35	73483.0
36	70331.0
37	96625.0
38	132579.0
39	87247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.048923120810155	31.848238482384822	20.536585365853657	27.56625303095136
2	24.002567394094992	33.056767936100414	18.62615889316788	24.314505776636715
3	20.440878619312507	34.19626301526173	19.62330623306233	25.73955213236343
4	20.067465411496222	36.06176009128512	18.204535729567823	25.666238767650835
5	18.059477963200685	36.760947083155045	16.73841106832121	28.44116388532306
6	19.2741406361432	40.823848238482384	17.91085437170161	21.9911567536728
7	81.29525032092427	11.215375837968907	3.8503779774639852	3.6389958636428474
8	82.74497218656397	9.767508201397805	3.740265297389816	3.74725431464841
9	77.56553986592498	12.538867493938097	5.968620738838967	3.9269719012979607
10	39.757809157038935	33.93267722150905	11.170018542290686	15.13949507916132
11	31.408643560119813	30.7732135216089	20.3594351732991	17.458707744972187
12	29.625445728141493	28.205391527599488	25.08415347311368	17.085009271145342
13	23.733704179147054	33.76265867921837	23.713735558408217	18.78990158322636
14	18.310512052488946	34.754956496933396	26.26757951790044	20.66695193267722
15	17.75538439594922	31.41106832120953	29.57338468121523	21.260162601626018
16	20.825417201540436	30.26772215090572	28.571530452146625	20.335330195407217
17	20.092140921409214	29.89545000713165	25.055341606047637	24.957067465411495
18	21.96633861075453	31.372557409784623	24.49379546427043	22.167308515190413
19	22.817715019255456	34.36727998858936	22.151333618599345	20.663671373555843
20	25.23334759663386	31.920125517044646	22.784624162031093	20.061902724290402
21	23.59891598915989	32.19169875909285	24.10226786478391	20.107117386963345
22	23.34146341463415	30.2634431607474	23.968050206817857	22.4270432178006
23	22.252317786335755	32.904293253458846	22.102981029810298	22.740407930395094
24	20.61560405077735	31.949935815147622	25.858080159748965	21.57637997432606
25	20.93025246041934	31.215375837968907	24.423049493652833	23.43132220795892
26	22.869062901155328	32.83240621879903	22.38040222507488	21.91812865497076
27	21.336043360433603	32.54471544715447	23.2886892026815	22.830551989730424
28	20.49550706033376	33.45014976465554	25.03337612323492	21.02096705177578
29	22.021680216802167	32.54385964912281	23.9867351305092	21.447725003565825
30	22.479104264726857	32.425046355726714	24.353159321066894	20.742690058479532
31	26.53530166880616	31.527028954500068	19.95906432748538	21.978605049208387
32	24.551276565397234	31.356867779204105	22.973755527028956	21.118100128369704
33	23.711025531307943	31.2179432320639	21.9500784481529	23.12095278847525
34	22.042504635572673	31.742832691484807	24.144201968335473	22.070460704607044
35	22.66909142775638	30.96077592354871	24.855512765653973	21.514619883040936
36	26.01526173156468	31.15247468264156	22.09128512337755	20.740978462416205
37	22.926686635287403	31.657823420339465	23.130794465839397	22.284695478533735
38	22.644986449864497	31.964341748680646	23.45756668092997	21.93310512052489
39	22.690629011553273	30.20225360148338	24.020111253744115	23.087006133219226
40	24.058907431179573	29.06133219226929	23.148053059477963	23.73170731707317
41	20.78719155612609	27.642989587790616	26.421623163600056	25.148195692483238
42	24.18399657680787	28.440593353301953	24.120382256454143	23.25502781343603
43	23.06247325631151	27.91984025103409	25.857652260733133	23.160034231921266
44	23.055484239052916	28.14534303237769	25.218228498074453	23.580944230494936
45	23.702182284980744	28.97846241620311	24.43531593210669	22.88403936670946
46	22.285693909570675	30.005420054200542	25.96690914277564	21.741976893453145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17126.0
1	13705.0
2	10284.0
3	7121.5
4	3959.0
5	3678.0
6	3397.0
7	3397.0
8	3410.0
9	3423.0
10	3490.5
11	3558.0
12	3501.5
13	3445.0
14	3512.0
15	3579.0
16	3519.5
17	3460.0
18	3494.5
19	3529.0
20	3529.0
21	3519.5
22	3510.0
23	3588.0
24	3666.0
25	4065.0
26	4464.0
27	4858.5
28	5253.0
29	6819.5
30	8386.0
31	8386.0
32	9703.5
33	11021.0
34	13611.0
35	16201.0
36	18460.0
37	20719.0
38	24493.5
39	28268.0
40	31026.0
41	33784.0
42	35678.0
43	37572.0
44	37572.0
45	43770.0
46	49968.0
47	52879.0
48	55790.0
49	52923.0
50	50056.0
51	49978.0
52	49900.0
53	45294.0
54	40688.0
55	40033.0
56	39378.0
57	39378.0
58	38010.5
59	36643.0
60	35695.0
61	34747.0
62	32689.0
63	30631.0
64	28824.0
65	27017.0
66	22521.5
67	18026.0
68	15619.5
69	13213.0
70	13213.0
71	11789.0
72	10365.0
73	8485.5
74	6606.0
75	5155.0
76	3704.0
77	3498.5
78	3293.0
79	2474.0
80	1655.0
81	1655.0
82	1027.0
83	399.0
84	310.5
85	222.0
86	165.5
87	109.0
88	74.5
89	40.0
90	35.5
91	31.0
92	22.0
93	13.0
94	13.0
95	7.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	701100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.780849484734382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95363280288065	16.761154933830618
2	9.088009558243817	3.9588913660787197
3	3.2359739073051017	2.1144678183462067
4	1.6153166798090732	1.4073187789240924
5	1.0275544506377634	1.1190504413355025
6	0.6714859880698654	0.8775321142354732
7	0.4766147065057399	0.7266751229228661
8	0.3962165693995633	0.6903946769159762
9	0.31758924376083664	0.622562716469286
>10	3.8899822638304395	20.459251505508888
>50	1.6031229205269484	25.243658328250646
>100	0.7165972627499259	21.45639946797675
>500	0.0026345487601100214	0.40239625390410166
>1k	0.004610460330192538	2.0993464455018267
>5k	0.0	0.0
>10k+	6.586371900275053E-4	2.060900029799046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14366	2.049065753815433	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3660	0.5220367993153616	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2869	0.4092140921409214	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	2695	0.38439594922265014	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1817	0.2591641705890743	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1307	0.1864213378975895	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	1152	0.16431322207958923	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	1134	0.16174582798459564	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT	941	0.13421765796605334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44701183853943804	0.0
2	0.0	0.0	0.0	0.5475681072600199	0.0
3	0.0	0.0	0.0	0.7215803736984738	0.0
4	0.0	0.0	0.0	1.5174725431464842	0.0
5	0.0	0.0	0.0	1.6468406789331052	0.0
6	0.0	0.0	0.0	2.3289117101697334	0.0
7	0.0	0.0	0.0	2.8375410069890172	0.0
8	0.0	0.0	0.0	3.169305377264299	0.0
9	0.0	0.0	0.0	3.8536585365853657	0.0
10	0.0	0.0	0.0	4.629011553273427	0.0
11	0.0	0.0	0.0	5.550278134360291	0.0
12	0.0	0.0	0.0	5.923833975181857	0.0
13	0.0	0.0	0.0	6.086578234203395	0.0
14	0.0	0.0	0.0	6.268863214947939	0.0
15	0.0	0.0	0.0	6.407930395093425	0.0
16	0.0	0.0	0.0	6.667807730708886	0.0
17	0.0	0.0	0.0	6.959777492511767	0.0
18	0.0	0.0	0.0	7.350021394950792	0.0
19	0.0	0.0	0.0	7.52545999144202	0.0
20	0.0	0.0	0.0	7.695193267722151	0.0
21	0.0	0.0	0.0	7.924119241192412	0.0
22	0.0	0.0	0.0	8.147910426472686	0.0
23	0.0	0.0	0.0	8.389102838396806	0.0
24	0.0	0.0	0.0	8.56083297675082	0.0
25	0.0	0.0	0.0	8.724860932819855	0.0
26	0.0	0.0	0.0	8.870774497218656	0.0
27	0.0	0.0	0.0	9.010697475395807	0.0
28	0.0	0.0	0.0	9.166595350164028	0.0
29	0.0	0.0	0.0	9.338325488518043	0.0
30	0.0	0.0	0.0	9.549850235344458	0.0
31	0.0	0.0	0.0	9.71188132933961	0.0
32	0.0	0.0	0.0	9.882042504635573	0.0
33	0.0	0.0	0.0	10.063043788332621	0.0
34	0.0	0.0	0.0	10.236628155755241	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	25	7.791538E-5	40.000004	9
TTTACGG	50	7.8216544E-11	40.000004	2
GAATCGG	25	7.791538E-5	40.000004	2
CGCTGTT	25	7.791538E-5	40.000004	14
TAACGGA	25	7.791538E-5	40.000004	13
CGTTCTA	25	7.791538E-5	40.000004	13
TAGCCGT	25	7.791538E-5	40.000004	30
CGTTAGC	25	7.791538E-5	40.000004	31
TACGCAG	25	7.791538E-5	40.000004	1
CCTCCGT	25	7.791538E-5	40.000004	37
CGTGTCA	25	7.791538E-5	40.000004	37
CCGGTTA	25	7.791538E-5	40.000004	19
ACGTACG	25	7.791538E-5	40.000004	1
ATCATTC	25	7.791538E-5	40.000004	19
GATTACG	25	7.791538E-5	40.000004	1
CGATACC	25	7.791538E-5	40.000004	33
CCGTTGC	25	7.791538E-5	40.000004	33
ATACACG	25	7.791538E-5	40.000004	1
TGTCCAA	25	7.791538E-5	40.000004	13
TCACGCC	35	3.0541014E-7	40.0	14
>>END_MODULE