##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548861_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 737607 Sequences flagged as poor quality 0 Sequence length 46 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.974056645340948 31.0 31.0 34.0 28.0 34.0 2 31.260454415427187 31.0 31.0 34.0 28.0 34.0 3 31.485427876904637 31.0 31.0 34.0 30.0 34.0 4 35.45379721179436 37.0 35.0 37.0 33.0 37.0 5 34.978243156586096 37.0 35.0 37.0 32.0 37.0 6 35.120434052279876 36.0 35.0 37.0 32.0 37.0 7 34.910192012819834 36.0 35.0 37.0 32.0 37.0 8 35.318740196337615 37.0 35.0 37.0 33.0 37.0 9 36.66576645829012 39.0 35.0 39.0 32.0 39.0 10 36.114030913481024 37.0 35.0 39.0 31.0 39.0 11 36.24150123304145 38.0 35.0 39.0 32.0 39.0 12 35.86275889464173 37.0 35.0 39.0 31.0 39.0 13 35.40190236806321 37.0 35.0 39.0 30.0 39.0 14 36.57019659520585 38.0 35.0 40.0 31.0 41.0 15 36.593436613264245 38.0 35.0 40.0 31.0 41.0 16 36.50856892627104 38.0 35.0 40.0 31.0 41.0 17 36.47984495808743 38.0 35.0 40.0 31.0 41.0 18 36.20876022055105 37.0 35.0 40.0 31.0 41.0 19 36.25108628307486 38.0 34.0 40.0 31.0 41.0 20 35.99409577186768 37.0 34.0 40.0 30.0 41.0 21 36.094505610711394 38.0 34.0 40.0 30.0 41.0 22 36.158737647554865 38.0 34.0 40.0 30.0 41.0 23 35.941151588854225 37.0 34.0 40.0 30.0 41.0 24 35.45068986601266 37.0 34.0 40.0 29.0 41.0 25 35.637531910624496 37.0 34.0 40.0 30.0 41.0 26 35.64441904699928 37.0 34.0 40.0 30.0 41.0 27 35.441723031370366 37.0 34.0 40.0 29.0 41.0 28 35.5092156121078 37.0 34.0 40.0 29.0 41.0 29 35.201231821281525 37.0 34.0 40.0 27.0 41.0 30 35.13228995928726 37.0 34.0 40.0 27.0 41.0 31 35.03616560038069 37.0 34.0 40.0 27.0 41.0 32 34.938425204749954 37.0 34.0 40.0 26.0 41.0 33 34.87326855629082 37.0 34.0 40.0 26.0 41.0 34 34.82633299304372 37.0 34.0 40.0 26.0 41.0 35 34.58073201582957 36.0 33.0 40.0 25.0 41.0 36 34.59316817763389 36.0 33.0 40.0 25.0 41.0 37 34.403432993450444 36.0 33.0 40.0 24.0 41.0 38 34.188159819524486 36.0 33.0 40.0 23.0 41.0 39 34.118750228780364 36.0 33.0 40.0 23.0 41.0 40 33.844686940335436 35.0 33.0 40.0 22.0 41.0 41 33.86774800130693 35.0 33.0 40.0 22.0 41.0 42 33.70049769050456 35.0 33.0 40.0 22.0 41.0 43 33.64440955685073 35.0 33.0 40.0 21.0 41.0 44 33.59490216334715 35.0 33.0 40.0 21.0 41.0 45 33.359074683401865 35.0 33.0 39.0 20.0 41.0 46 32.35243971383135 35.0 31.0 38.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 9.0 11 18.0 12 7.0 13 24.0 14 29.0 15 73.0 16 131.0 17 274.0 18 543.0 19 1056.0 20 1925.0 21 3029.0 22 4531.0 23 6129.0 24 7702.0 25 9402.0 26 10827.0 27 12290.0 28 13829.0 29 16981.0 30 20757.0 31 25906.0 32 34614.0 33 48302.0 34 79568.0 35 90110.0 36 70710.0 37 90511.0 38 115955.0 39 72358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.238419646234377 34.443680713442255 18.964841711100895 26.353057929222473 2 19.542249463467673 38.50492199775761 17.392324096707327 24.560504442067387 3 18.53466683477787 38.7618338763054 18.767175474202386 23.93632381471434 4 17.98654296935902 38.02282245152229 18.442070099660118 25.548564479458573 5 14.97830145321289 41.34898394402439 16.705644062488563 26.967070540274158 6 19.31868867838836 40.67179405835357 18.957385165813232 21.052132097444844 7 73.3215655491339 16.393418175261353 5.707782057382861 4.577234218221898 8 75.6543796357681 14.860759184769126 4.39502336610146 5.089837813361315 9 71.04325202987499 16.580916395858498 7.920206831008924 4.455624743257588 10 39.87191010931295 33.73286858720159 10.904180681582469 15.491040621902991 11 31.540915419728933 31.63663034651244 20.022450979993412 16.800003253765215 12 30.031439506403817 29.2253191740317 24.38358095842366 16.359660361140822 13 20.763902728688855 38.565116654261686 22.668439968709624 18.002540648339835 14 15.063034922390923 40.23782312261137 27.297734430394506 17.40140752460321 15 15.165257379607297 34.342000550428615 32.734233812856985 17.758508257107106 16 15.42976137699344 31.172562082518198 31.611955960287798 21.785720580200568 17 16.577255909990008 31.061256197405935 24.559555427212594 27.80193246539146 18 19.0113434389858 32.726506120467945 25.093172922708163 23.16897751783809 19 21.583309269028085 35.888081322438644 21.793583846140287 20.73502556239298 20 21.581546812869185 34.70249062169963 23.221580055503814 20.494382509927373 21 18.470269398202564 35.22119502661987 24.906081422763073 21.402454152414496 22 19.80160166592779 34.12576073708628 22.57719896909872 23.495438627887207 23 19.008903115073476 35.32192617477871 21.520538715061 24.148631995086813 24 17.267054135874524 35.303623745436255 26.66162333058119 20.76769878810803 25 17.509324070948352 33.98218834691102 25.902818167398085 22.60566941474254 26 19.17457399401036 37.60756066577459 21.584258283882882 21.633607056332167 27 16.403043897359975 36.503991963199915 23.55414197533375 23.53882216410636 28 17.567349550641467 34.997227520888494 27.375960369139662 20.059462559330374 29 17.99820229471792 33.35082232137168 26.753271050844145 21.897704333066255 30 18.502400329714874 35.01078487595698 23.713440897388445 22.773373896939695 31 22.691351898775363 34.41534584134912 21.57273453207467 21.32056772780085 32 23.212496627607926 31.402223677378334 22.216708897827704 23.168570797186035 33 20.704657087039575 31.831178391745198 24.126669079875867 23.33749544133936 34 18.032231255939816 31.57263963058919 23.552650666276215 26.84247844719478 35 18.00715014906312 32.24481329488467 25.654718569644814 24.093317986407396 36 23.988790778829376 29.23141998381252 25.104425527415003 21.6753637099431 37 18.99873509877211 33.0651688500787 24.403645843925016 23.532450207224173 38 20.508075438546545 32.485998641553024 23.994349294407456 23.011576625492978 39 20.462793872617805 30.206871680990012 25.4973176772997 23.833016769092485 40 21.967389138118264 30.67758304896781 22.130890840244195 25.22413697266973 41 18.821540468027013 28.019392440689963 27.21462784382469 25.944439247458334 42 21.608119228803414 29.414173130135694 24.00004338353622 24.97766425752467 43 19.949241262623595 28.778468750974433 26.791367218586593 24.48092276781538 44 20.4995343048534 28.028340295035157 25.268198376642303 26.203927023469138 45 20.700454306968343 32.99073897075272 24.229976125497725 22.078830596781213 46 20.54942537150542 30.859387180436194 27.565763340098453 21.02542410795993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 33468.0 1 26198.5 2 18929.0 3 12750.5 4 6572.0 5 5710.0 6 4848.0 7 4848.0 8 4843.0 9 4838.0 10 4855.0 11 4872.0 12 4806.0 13 4740.0 14 4564.0 15 4388.0 16 4612.5 17 4837.0 18 4429.0 19 4021.0 20 4021.0 21 3890.5 22 3760.0 23 4246.0 24 4732.0 25 4698.5 26 4665.0 27 4559.0 28 4453.0 29 5902.5 30 7352.0 31 7352.0 32 9081.5 33 10811.0 34 11608.5 35 12406.0 36 14590.5 37 16775.0 38 19317.0 39 21859.0 40 27281.0 41 32703.0 42 38286.5 43 43870.0 44 43870.0 45 53337.0 46 62804.0 47 68825.5 48 74847.0 49 68930.0 50 63013.0 51 56771.5 52 50530.0 53 44623.0 54 38716.0 55 36502.0 56 34288.0 57 34288.0 58 32500.0 59 30712.0 60 29430.5 61 28149.0 62 26962.5 63 25776.0 64 22480.0 65 19184.0 66 18752.5 67 18321.0 68 16609.0 69 14897.0 70 14897.0 71 12075.0 72 9253.0 73 6992.0 74 4731.0 75 4408.5 76 4086.0 77 2909.5 78 1733.0 79 1283.0 80 833.0 81 833.0 82 628.5 83 424.0 84 251.0 85 78.0 86 120.5 87 163.0 88 96.5 89 30.0 90 24.5 91 19.0 92 49.5 93 80.0 94 80.0 95 58.5 96 37.0 97 20.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 737607.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.159453766115014 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93408785057653 17.048173627630813 2 10.069285612829681 4.4625973799061285 3 3.7389392830128827 2.485585565287056 4 1.850350066472462 1.6401098699649743 5 1.1540858933018558 1.278695649737401 6 0.764015274367222 1.0158096689367682 7 0.5423816698992099 0.8413217076403859 8 0.3888058644208297 0.6892580461302207 9 0.2781808524326396 0.5547902164289588 >10 2.4434314181585917 12.06569823984111 >50 0.8508964582882099 13.659402690091445 >100 0.9732435646461245 35.09393341125698 >500 0.006148095796880129 0.9230154618981414 >1k 0.00430366705781609 2.6416634400302526 >5k 0.0012296191593760258 1.7200103625777174 >10k+ 6.148095796880129E-4 3.8799346626416487 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28479 3.8609991499538374 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7184 0.9739603881199609 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5441 0.7376556892762678 No Hit CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT 4719 0.6397715856818061 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3571 0.4841331494955986 No Hit AATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT 2833 0.38407986909017944 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTAT 2807 0.38055495677237333 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGT 1961 0.2658597328929905 No Hit AAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT 1915 0.25962334956148736 No Hit AAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC 1584 0.21474850428480208 No Hit AAAAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC 867 0.11754226844376475 No Hit AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 0.10520507533144344 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.711471013696996E-4 0.0 0.0 0.7190821128324433 0.0 2 2.711471013696996E-4 0.0 0.0 0.9628433569638032 0.0 3 2.711471013696996E-4 0.0 0.0 1.3353994742457704 0.0 4 2.711471013696996E-4 0.0 0.0 3.06884289330226 0.0 5 2.711471013696996E-4 0.0 0.0 3.343379333439081 0.0 6 2.711471013696996E-4 0.0 0.0 4.7025041790547 0.0 7 2.711471013696996E-4 0.0 0.0 5.7721794939581645 0.0 8 2.711471013696996E-4 0.0 0.0 6.328708919519473 0.0 9 2.711471013696996E-4 0.0 0.0 7.509961266636569 0.0 10 4.0672065205454936E-4 0.0 0.0 8.831396665161801 0.0 11 4.0672065205454936E-4 0.0 0.0 10.321485560738985 0.0 12 4.0672065205454936E-4 0.0 0.0 11.015486566694731 0.0 13 4.0672065205454936E-4 0.0 0.0 11.270229268431564 0.0 14 4.0672065205454936E-4 0.0 0.0 11.519549028141002 0.0 15 4.0672065205454936E-4 0.0 0.0 11.715317235329925 0.0 16 4.0672065205454936E-4 0.0 0.0 12.089093514568056 0.0 17 4.0672065205454936E-4 0.0 0.0 12.547060968781478 0.0 18 4.0672065205454936E-4 0.0 0.0 13.210693499383819 0.0 19 4.0672065205454936E-4 0.0 0.0 13.462453583005585 0.0 20 4.0672065205454936E-4 0.0 0.0 13.714620387279405 0.0 21 4.0672065205454936E-4 0.0 0.0 13.997020093355948 0.0 22 4.0672065205454936E-4 0.0 0.0 14.266269165016059 0.0 23 4.0672065205454936E-4 0.0 0.0 14.565208844276153 0.0 24 4.0672065205454936E-4 0.0 0.0 14.775212274286984 0.0 25 4.0672065205454936E-4 0.0 0.0 14.959456729667696 0.0 26 4.0672065205454936E-4 0.0 0.0 15.121195975634722 0.0 27 4.0672065205454936E-4 0.0 0.0 15.317235329925014 0.0 28 4.0672065205454936E-4 0.0 0.0 15.494023240018059 0.0 29 4.0672065205454936E-4 0.0 0.0 15.693723080176841 0.0 30 4.0672065205454936E-4 0.0 0.0 15.96961525582051 0.0 31 4.0672065205454936E-4 0.0 0.0 16.16185855069163 0.0 32 4.0672065205454936E-4 0.0 0.0 16.347052020927133 0.0 33 4.0672065205454936E-4 0.0 0.0 16.51502765022566 0.0 34 4.0672065205454936E-4 0.0 0.0 16.685579176987204 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGAC 30 4.865138E-6 40.000004 17 TAAAGCG 30 4.865138E-6 40.000004 1 TCTTCGA 30 4.865138E-6 40.000004 37 CCAATTG 30 4.865138E-6 40.000004 1 GACCGAA 30 4.865138E-6 40.000004 9 GGATCGA 30 4.865138E-6 40.000004 8 TGTGCGA 30 4.865138E-6 40.000004 35 ACGGCAG 30 4.865138E-6 40.000004 1 TATCTAG 30 4.865138E-6 40.000004 1 CTAGTCG 30 4.865138E-6 40.000004 2 TCGTCTA 30 4.865138E-6 40.000004 13 TCCACGT 30 4.865138E-6 40.000004 17 ATCTTCG 30 4.865138E-6 40.000004 25 TAGGGTG 60 0.0 40.000004 5 CGACTAG 60 0.0 40.000004 1 ACTCACG 30 4.865138E-6 40.000004 1 ACGAGGT 30 4.865138E-6 40.000004 16 CCAAACG 30 4.865138E-6 40.000004 1 TAGCTAC 30 4.865138E-6 40.000004 24 ACGGTGA 30 4.865138E-6 40.000004 28 >>END_MODULE