FastQCFastQC Report
Sat 18 Jun 2016
SRR3548859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592190
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207503.5039429912696938No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46850.7911312247758321No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41780.705516810483122No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24840.4194599706175383No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTAT24600.41540721727823837No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGT20380.3441463043955487No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT19970.33722285077424474No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGT18870.3186477313024536No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC6330.10689136932403452No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC6120.10334521015214712No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.10064337459261385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA200.001255275640.00000411
TAGAGCG200.001255275640.0000041
CCCCTAG200.001255275640.0000041
AGATCTA200.001255275640.00000415
ACACGAA200.001255275640.00000433
ACGCCTC200.001255275640.00000414
AATCACG200.001255275640.0000041
TACCACG401.9255822E-840.0000041
CCCTAGA200.001255275640.00000422
CCCTACC200.001255275640.00000413
ACACCGT200.001255275640.00000413
GAACTAT200.001255275640.0000049
CCGGATC200.001255275640.00000439
ATAGATC200.001255275640.00000439
ATTCGCA200.001255275640.00000429
TACGGTC200.001255275640.00000428
CGATTCG200.001255275640.0000041
CACGACT200.001255275640.00000426
CACGAAC200.001255275640.00000434
CGTTCCC200.001255275640.00000440
Produced by FastQC (version 0.11.5)