##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592190
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14411759739273	31.0	31.0	34.0	28.0	34.0
2	31.4314105270268	31.0	31.0	34.0	30.0	34.0
3	31.61311234569986	31.0	31.0	34.0	30.0	34.0
4	35.569146726557356	37.0	35.0	37.0	33.0	37.0
5	35.11932150154511	37.0	35.0	37.0	32.0	37.0
6	35.26103953123153	37.0	35.0	37.0	32.0	37.0
7	35.0147486448606	37.0	35.0	37.0	32.0	37.0
8	35.47616812171769	37.0	35.0	37.0	33.0	37.0
9	36.812198787551296	39.0	37.0	39.0	32.0	39.0
10	36.252569276752396	37.0	35.0	39.0	32.0	39.0
11	36.42903797767608	38.0	35.0	39.0	32.0	39.0
12	36.132710785389826	37.0	35.0	39.0	32.0	39.0
13	35.82137320792313	38.0	35.0	39.0	30.0	39.0
14	37.005717759502865	39.0	35.0	40.0	31.0	41.0
15	36.968874854354176	39.0	35.0	40.0	32.0	41.0
16	36.828754284942335	38.0	35.0	40.0	32.0	41.0
17	36.749916411962374	38.0	35.0	40.0	32.0	41.0
18	36.488529019402556	38.0	35.0	40.0	31.0	41.0
19	36.56738040156031	38.0	35.0	40.0	31.0	41.0
20	36.30379439031392	38.0	34.0	40.0	30.0	41.0
21	36.386487444907885	38.0	35.0	40.0	31.0	41.0
22	36.45604282409362	38.0	35.0	40.0	31.0	41.0
23	36.2068204461406	38.0	35.0	40.0	31.0	41.0
24	35.79947314206589	38.0	34.0	40.0	30.0	41.0
25	35.9933433526402	38.0	34.0	40.0	30.0	41.0
26	36.02520474847599	38.0	34.0	40.0	30.0	41.0
27	35.91839443421875	38.0	34.0	40.0	30.0	41.0
28	35.89346831253483	38.0	34.0	40.0	30.0	41.0
29	35.586845438119525	37.0	34.0	40.0	29.0	41.0
30	35.571728668163935	37.0	34.0	40.0	29.0	41.0
31	35.44208615478141	37.0	34.0	40.0	29.0	41.0
32	35.31541397186714	37.0	34.0	40.0	27.0	41.0
33	35.21685945389149	37.0	34.0	40.0	27.0	41.0
34	35.10534794576065	37.0	34.0	40.0	27.0	41.0
35	34.841341461355306	37.0	34.0	40.0	25.0	41.0
36	34.76824161164491	37.0	34.0	40.0	25.0	41.0
37	34.61545112210608	37.0	33.0	40.0	24.0	41.0
38	34.360460325233454	37.0	33.0	40.0	23.0	41.0
39	34.29675442003411	37.0	33.0	40.0	23.0	41.0
40	34.07827724210135	36.0	33.0	40.0	22.0	41.0
41	34.0997061753829	36.0	33.0	40.0	22.0	41.0
42	33.92610986338844	36.0	33.0	40.0	21.0	41.0
43	33.84956348469241	36.0	33.0	40.0	21.0	41.0
44	33.67561255678076	36.0	33.0	40.0	21.0	41.0
45	33.42177172866816	35.0	33.0	40.0	20.0	41.0
46	32.39453047163917	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	5.0
11	11.0
12	12.0
13	16.0
14	24.0
15	49.0
16	101.0
17	167.0
18	377.0
19	798.0
20	1405.0
21	2313.0
22	3607.0
23	4914.0
24	6439.0
25	7718.0
26	8621.0
27	9259.0
28	10032.0
29	12160.0
30	14722.0
31	18618.0
32	24881.0
33	35162.0
34	57649.0
35	65948.0
36	57060.0
37	76389.0
38	105311.0
39	68416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.259713943160136	34.66927844104088	18.776068491531433	26.294939124267547
2	20.90460831827623	37.274016785153414	18.126952498353567	23.694422398216787
3	18.78113443320556	38.14130599976359	18.921123288133877	24.156436278896976
4	18.979043887941373	38.56768942400243	16.908762390449013	25.544504297607183
5	16.634188351711444	41.13493979972644	15.47155473749979	26.75931711106233
6	18.577652442628214	42.929634070146406	18.331954271433155	20.16075921579223
7	76.06123034836793	16.996909775578782	3.743393167733329	3.198466708319965
8	77.41653861091879	14.566270960333677	3.429642513382529	4.587547915365001
9	72.73341326263531	17.32433847244972	5.99148921798747	3.9507590469275065
10	38.40861885543491	37.24497205288843	11.270538171870514	13.075870919806142
11	30.48447288876881	33.24946385450615	20.097266080143196	16.16879717658184
12	28.497272834732097	31.469291950218683	23.61488711393303	16.418548101116194
13	20.711764805214543	39.22980800081055	21.529576656140765	18.528850537834142
14	16.36265387797835	40.49460477211706	25.601749438524795	17.540991911379795
15	18.008240598456577	34.90315608166298	28.059744338810177	19.028858981070265
16	19.071750620577856	33.24237153616238	28.108039649436833	19.577838193822927
17	17.96011415255239	33.123828500987855	24.321248248028503	24.59480909843125
18	21.3794559178642	33.970853948901535	24.163022003073337	20.48666813016093
19	21.552204528951858	36.435265708640806	22.542089532075853	19.47044023033148
20	23.542444147993045	36.32178861514041	21.513872236950977	18.62189499991557
21	20.830983299278948	35.7586247657002	23.133116060723754	20.2772758742971
22	21.215994866512435	33.99331295699016	21.93569631368311	22.854995862814302
23	19.721879802090545	36.99015518667995	20.043904827842415	23.244060183387088
24	18.43411742852801	36.29088637092825	24.764687009236898	20.510309191306845
25	18.63928806633006	35.066110538847326	24.37005015282257	21.924551242000035
26	20.551174454144785	37.23044968675594	21.424036204596497	20.794339654502778
27	18.05113223796417	36.75290025160844	23.50546277377193	21.690504736655466
28	18.981407994055964	37.1721913575035	25.585031831000187	18.26136881744035
29	18.678295817220825	36.01310390246374	25.143112852294024	20.16548742802141
30	20.512504432698965	35.792397710194365	24.25539100626488	19.43970685084179
31	24.705077762204695	34.63246593154224	21.18830105202722	19.47415525422584
32	23.390128168324356	34.15880038501157	21.549502693392323	20.901568753271754
33	22.555767574595993	32.69524983535689	22.910045762339788	21.83893682770732
34	20.571438220841284	33.17144835272463	23.53788480048633	22.719228625947753
35	19.715631807359124	34.27953866157821	25.309444603927794	20.69538492713487
36	24.00006754588899	31.825596514632124	23.849440213445007	20.324895726033873
37	21.64068964352657	33.237305594488255	22.634458535267395	22.487546226717775
38	22.67346628615816	32.16096185345919	23.360576841891962	21.804995018490686
39	20.803627214238674	30.141339772708083	24.627399989868117	24.427633023185127
40	22.295884766713385	30.37521741333018	23.649166652594605	23.679731167361826
41	19.546935949610766	28.60821695739543	26.414664212499368	25.430182880494435
42	22.97978689272024	28.899339738935137	23.443320555902666	24.677552812441952
43	22.333879329269323	28.145696482547834	25.90958982758912	23.610834360593728
44	22.119083402286428	29.732518279606207	26.4202367483409	21.72816156976646
45	22.350259207348994	31.502389435822963	24.075381212110976	22.071970144717067
46	21.274928654654758	29.552001891284892	27.56125567807629	21.61181377598406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24369.0
1	19381.0
2	14393.0
3	10045.5
4	5698.0
5	5348.0
6	4998.0
7	4998.0
8	5086.0
9	5174.0
10	5131.5
11	5089.0
12	5086.5
13	5084.0
14	4763.0
15	4442.0
16	4404.0
17	4366.0
18	4058.5
19	3751.0
20	3751.0
21	3456.5
22	3162.0
23	3460.0
24	3758.0
25	3814.0
26	3870.0
27	4610.0
28	5350.0
29	5639.5
30	5929.0
31	5929.0
32	7441.0
33	8953.0
34	10661.0
35	12369.0
36	14110.5
37	15852.0
38	16711.5
39	17571.0
40	22591.5
41	27612.0
42	29561.0
43	31510.0
44	31510.0
45	39117.5
46	46725.0
47	48971.5
48	51218.0
49	48157.5
50	45097.0
51	41435.0
52	37773.0
53	35213.5
54	32654.0
55	30117.0
56	27580.0
57	27580.0
58	26824.5
59	26069.0
60	23959.5
61	21850.0
62	22080.0
63	22310.0
64	20091.0
65	17872.0
66	16978.5
67	16085.0
68	13452.0
69	10819.0
70	10819.0
71	9644.5
72	8470.0
73	6669.0
74	4868.0
75	4291.0
76	3714.0
77	3502.5
78	3291.0
79	2342.0
80	1393.0
81	1393.0
82	987.0
83	581.0
84	453.5
85	326.0
86	241.5
87	157.0
88	93.0
89	29.0
90	17.5
91	6.0
92	4.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	592190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.297956566469217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41203880426886	18.035423177836854
2	8.982545071163962	4.185498898486602
3	3.28001826136816	2.292531689717438
4	1.7119130241519356	1.5953630112905903
5	1.062282121495748	1.237450136397236
6	0.6983517634012506	0.9762101431103714
7	0.523244861847273	0.8533375245462167
8	0.399567042650006	0.7447276484041914
9	0.32760786010184945	0.686933432593813
>10	3.6463405033970275	20.086623156774607
>50	1.3503054532343748	22.587277373654263
>100	0.5963425374424001	19.366543120469064
>500	0.0036318059527551765	0.5019282153713376
>1k	0.005084528333857247	3.338685691524316
>5k	0.0	0.0
>10k+	7.263611905510353E-4	3.5114667798230803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20750	3.5039429912696938	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4685	0.7911312247758321	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4178	0.705516810483122	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2484	0.4194599706175383	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTAT	2460	0.41540721727823837	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGT	2038	0.3441463043955487	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT	1997	0.33722285077424474	No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGT	1887	0.3186477313024536	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	633	0.10689136932403452	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	612	0.10334521015214712	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.10064337459261385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38146540806160184	0.0
2	0.0	0.0	0.0	0.5079450851922525	0.0
3	0.0	0.0	0.0	0.6989310863067597	0.0
4	0.0	0.0	0.0	1.6528479035444705	0.0
5	0.0	0.0	0.0	1.8374170452050862	0.0
6	0.0	0.0	0.0	2.6891707053479457	0.0
7	0.0	0.0	0.0	3.3544977118830106	0.0
8	0.0	0.0	0.0	3.8227595872946183	0.0
9	0.0	0.0	0.0	4.781742346206454	0.0
10	0.0	0.0	0.0	5.727384792043094	0.0
11	0.0	0.0	0.0	6.944561711612827	0.0
12	0.0	0.0	0.0	7.420591364258093	0.0
13	0.0	0.0	0.0	7.642648474307232	0.0
14	0.0	0.0	0.0	7.864198990188959	0.0
15	0.0	0.0	0.0	8.00283692733751	0.0
16	0.0	0.0	0.0	8.274877995238015	0.0
17	0.0	0.0	0.0	8.57950995457539	0.0
18	0.0	0.0	0.0	9.05233117749371	0.0
19	0.0	0.0	0.0	9.234367348317264	0.0
20	0.0	0.0	0.0	9.418767625255407	0.0
21	0.0	0.0	0.0	9.611273408872153	0.0
22	0.0	0.0	0.0	9.807325351660785	0.0
23	0.0	0.0	0.0	10.019081713639203	0.0
24	0.0	0.0	0.0	10.164305374964115	0.0
25	0.0	0.0	0.0	10.318647731302454	0.0
26	0.0	0.0	0.0	10.43600871341968	0.0
27	0.0	0.0	0.0	10.571269356118814	0.0
28	0.0	0.0	0.0	10.696566980192168	0.0
29	0.0	0.0	0.0	10.82338438676776	0.0
30	0.0	0.0	0.0	11.039531231530422	0.0
31	0.0	0.0	0.0	11.17614279200932	0.0
32	0.0	0.0	0.0	11.32052212972188	0.0
33	0.0	0.0	0.0	11.468109897161384	0.0
34	0.0	0.0	0.0	11.61603539404583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	0.0012552756	40.000004	11
TAGAGCG	20	0.0012552756	40.000004	1
CCCCTAG	20	0.0012552756	40.000004	1
AGATCTA	20	0.0012552756	40.000004	15
ACACGAA	20	0.0012552756	40.000004	33
ACGCCTC	20	0.0012552756	40.000004	14
AATCACG	20	0.0012552756	40.000004	1
TACCACG	40	1.9255822E-8	40.000004	1
CCCTAGA	20	0.0012552756	40.000004	22
CCCTACC	20	0.0012552756	40.000004	13
ACACCGT	20	0.0012552756	40.000004	13
GAACTAT	20	0.0012552756	40.000004	9
CCGGATC	20	0.0012552756	40.000004	39
ATAGATC	20	0.0012552756	40.000004	39
ATTCGCA	20	0.0012552756	40.000004	29
TACGGTC	20	0.0012552756	40.000004	28
CGATTCG	20	0.0012552756	40.000004	1
CACGACT	20	0.0012552756	40.000004	26
CACGAAC	20	0.0012552756	40.000004	34
CGTTCCC	20	0.0012552756	40.000004	40
>>END_MODULE