##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563691
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23260793590815	31.0	31.0	34.0	28.0	34.0
2	31.458318475902576	31.0	31.0	34.0	30.0	34.0
3	31.412522108743975	31.0	31.0	34.0	28.0	34.0
4	35.40346927660722	37.0	35.0	37.0	33.0	37.0
5	35.083978633684055	37.0	35.0	37.0	32.0	37.0
6	35.12522108743975	37.0	35.0	37.0	32.0	37.0
7	35.11256344344685	37.0	35.0	37.0	32.0	37.0
8	35.49202311195318	37.0	35.0	37.0	33.0	37.0
9	35.941047488783745	39.0	37.0	39.0	32.0	39.0
10	35.68246787690419	38.0	35.0	39.0	30.0	39.0
11	36.14216299355498	38.0	35.0	39.0	31.0	39.0
12	35.95388963102125	37.0	35.0	39.0	31.0	39.0
13	35.86039514556734	37.0	35.0	39.0	31.0	39.0
14	36.884975988617875	39.0	35.0	40.0	31.0	41.0
15	36.93106329531605	38.0	35.0	40.0	32.0	41.0
16	36.89968617558201	38.0	35.0	40.0	32.0	41.0
17	36.897809260747465	38.0	35.0	40.0	32.0	41.0
18	36.69728982722804	38.0	35.0	40.0	31.0	41.0
19	36.76487295344435	38.0	35.0	40.0	31.0	41.0
20	36.79436251421435	38.0	35.0	40.0	31.0	41.0
21	36.87470440365377	39.0	35.0	40.0	31.0	41.0
22	36.86678517130839	39.0	35.0	40.0	31.0	41.0
23	36.82184388255268	38.0	35.0	40.0	31.0	41.0
24	36.43502912056428	38.0	35.0	40.0	30.0	41.0
25	36.52505894186709	38.0	35.0	40.0	31.0	41.0
26	36.454795269039245	38.0	35.0	40.0	31.0	41.0
27	36.331137094613894	38.0	35.0	40.0	30.0	41.0
28	36.258226581584594	38.0	35.0	40.0	30.0	41.0
29	36.11855963639653	38.0	35.0	40.0	30.0	41.0
30	36.05415555685651	38.0	35.0	40.0	30.0	41.0
31	36.0350724066909	38.0	35.0	40.0	30.0	41.0
32	35.92502452584838	38.0	35.0	40.0	30.0	41.0
33	35.828695153905244	38.0	34.0	40.0	30.0	41.0
34	35.79678760171796	38.0	34.0	40.0	29.0	41.0
35	35.676452169717095	38.0	34.0	40.0	29.0	41.0
36	35.487543707456744	38.0	34.0	40.0	28.0	41.0
37	35.357243596225594	38.0	34.0	40.0	27.0	41.0
38	35.376958298074655	38.0	34.0	40.0	28.0	41.0
39	35.191393866497776	38.0	34.0	40.0	27.0	41.0
40	35.02890945571244	37.0	34.0	40.0	26.0	41.0
41	34.95160114317951	37.0	34.0	40.0	26.0	41.0
42	35.02149759353972	37.0	34.0	40.0	26.0	41.0
43	34.87129296015015	37.0	33.0	40.0	26.0	41.0
44	34.888373239948834	37.0	33.0	40.0	26.0	41.0
45	34.66721129129257	37.0	33.0	40.0	26.0	41.0
46	34.39847540585179	37.0	33.0	40.0	24.0	41.0
47	34.28979884369273	37.0	33.0	40.0	24.0	41.0
48	34.14818224878524	36.0	33.0	40.0	23.0	41.0
49	34.07153919434584	36.0	33.0	40.0	24.0	41.0
50	33.90685499679789	36.0	33.0	40.0	23.0	41.0
51	32.04103844127368	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	5.0
11	8.0
12	10.0
13	8.0
14	23.0
15	43.0
16	61.0
17	174.0
18	357.0
19	697.0
20	1062.0
21	1601.0
22	2172.0
23	2904.0
24	3649.0
25	4751.0
26	5755.0
27	7424.0
28	9577.0
29	12191.0
30	15828.0
31	20169.0
32	26350.0
33	35204.0
34	53166.0
35	58357.0
36	59542.0
37	78078.0
38	95137.0
39	69381.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.96748395840984	35.96243331896376	20.301725590793538	24.768357131832865
2	21.74116670303411	34.19515301823162	20.39397471309636	23.669705565637912
3	20.72039468432173	30.07729412036027	24.088906865640926	25.113404329677074
4	22.692751880019372	31.668059273609124	18.58748853538552	27.051700310985982
5	17.41876311667208	37.54237694055786	18.128371749770707	26.910488192999356
6	19.876137813092633	36.306238701700046	22.084262477137298	21.733361008070023
7	81.2569297718076	8.508030108694301	5.734169961911757	4.500870157586337
8	83.74499504160967	4.521448807946197	3.900896058301445	7.832660092142681
9	77.00797067897128	10.731943564825409	7.46206698350692	4.7980187726963885
10	38.67065466718468	36.03782923623049	11.741361845408212	13.550154251176618
11	29.484061303089813	24.314562410966293	27.41413292034111	18.787243365602784
12	30.2807743959013	22.549588338291723	30.136546441224006	17.03309082458297
13	24.583149278594124	31.442226326125482	23.5724891829034	20.402135212376994
14	18.592278393658937	31.32354428223974	30.47094950957173	19.61322781452959
15	16.79891997566042	30.550780480795332	29.968901401654453	22.681398141889794
16	19.0503307663241	27.041410985806053	32.38281256929772	21.525445678572126
17	19.101422587907205	27.257309412426313	27.699218188688484	25.942049810978002
18	20.753391485760815	28.217055088692213	27.25340656494427	23.776146860602708
19	21.877766364905597	30.688976762091286	27.437550005233362	19.99570686776975
20	24.79549966204889	29.977771509568186	25.2952415419086	19.93148728647433
21	21.89586138504961	28.69586351387551	26.083971537597726	23.32430356347715
22	21.398248331089196	28.77463007214946	25.707169353422355	24.119952243338993
23	21.946421000157887	28.963740772870246	23.004447472107945	26.085390754863923
24	18.44237356991685	31.994124440517947	27.745697554156447	21.81780443540876
25	20.60082562964461	28.60308218509786	25.91384286781233	24.882249317445197
26	23.753261982185276	31.17310725202283	22.45538779224788	22.618242973544017
27	18.17236748502282	30.56621446856522	26.83899512321467	24.422422923197285
28	19.353333652657216	30.960934270726337	27.567408385090413	22.118323691526033
29	21.226877846195876	29.42622819949228	26.84768782897013	22.49920612534172
30	22.663657926062328	30.223118694462038	24.859009634711214	22.25421374476442
31	24.99667370953235	31.099840160655397	20.835706087200258	23.067780042612
32	24.446904421039186	29.761518278631378	22.404295970664776	23.387281329664656
33	26.0715533865185	29.48938336783805	21.66151313396879	22.777550111674657
34	21.182704708785487	31.22490868223903	23.025203524626082	24.567183084349402
35	24.00180240592807	29.942113675754978	24.370976297297634	21.685107621019316
36	25.41090774910368	29.578794055608483	24.735360330393778	20.274937864894063
37	21.919633274258416	33.64946397937877	22.515704526061263	21.915198220301548
38	23.917358978589334	29.063972992295422	24.67823683542934	22.3404311936859
39	22.095261410950325	30.62617639806206	24.853332765646428	22.42522942534119
40	26.89452199875464	28.092696175741672	22.476853453399116	22.535928372104575
41	21.12416199655485	26.828883200193015	28.418761342650495	23.628193460601643
42	26.10508239443241	26.49873778364388	22.531670720305982	24.86450910161773
43	23.01722042750372	26.341382069254255	25.970079351985397	24.671318151256628
44	21.121500964180733	27.279129877894093	24.565586464924934	27.03378269300024
45	24.532589663485847	29.200572654166912	23.749004330386683	22.517833351960558
46	20.763148604465922	28.167205082217027	29.30080487359209	21.768841439724955
47	23.1832688476488	28.587470795169693	24.072231062763112	24.157029294418393
48	21.760326136127773	24.975030646222844	29.95701545705005	23.30762776059934
49	23.036734664913933	24.366541243340766	28.026347768546948	24.57037632319835
50	21.469031792240784	26.17143080162713	25.34544635270033	27.014091053431756
51	20.249391954102514	25.478320569248048	29.443436208844915	24.828851267804524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1988.0
1	1592.0
2	1196.0
3	828.0
4	460.0
5	469.0
6	478.0
7	515.0
8	552.0
9	634.0
10	716.0
11	765.0
12	814.0
13	923.5
14	1033.0
15	1084.5
16	1136.0
17	1301.5
18	1467.0
19	1453.5
20	1440.0
21	1331.0
22	1222.0
23	1637.5
24	2053.0
25	2204.0
26	3054.0
27	3753.0
28	4754.0
29	5755.0
30	6940.0
31	8125.0
32	9959.0
33	11793.0
34	13909.0
35	16025.0
36	16515.5
37	17006.0
38	17937.5
39	18869.0
40	22254.0
41	25639.0
42	30933.0
43	36227.0
44	47360.5
45	58494.0
46	59799.5
47	61105.0
48	58940.0
49	56775.0
50	52559.0
51	48343.0
52	44158.0
53	39973.0
54	35550.5
55	31128.0
56	27756.5
57	24385.0
58	22479.5
59	20574.0
60	19209.5
61	17845.0
62	15833.0
63	13821.0
64	12244.5
65	10668.0
66	8981.0
67	7294.0
68	6200.5
69	5107.0
70	4085.5
71	3064.0
72	2507.0
73	1950.0
74	1625.5
75	977.0
76	653.0
77	590.0
78	527.0
79	394.5
80	262.0
81	198.5
82	135.0
83	116.0
84	97.0
85	69.0
86	41.0
87	30.0
88	19.0
89	18.5
90	18.0
91	11.0
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.802713450664953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59177605356057	24.99431733410219
2	8.89487657838332	5.657624220027118
3	2.9948362143820955	2.85731753873004
4	1.4534535967498614	1.8489507300509669
5	0.8664401885014948	1.3777574518526585
6	0.6361209621287275	1.213822360712473
7	0.4921146247726307	1.0955406277567836
8	0.4137804754187081	1.0527473512976873
9	0.3281167366156459	0.9391502297659221
>10	4.415148507358761	33.32616019707378
>50	0.8424352797991727	17.44054482394157
>100	0.06364322193313825	3.2636550066037584
>500	0.003907917136245331	0.8674909347332153
>1k	0.0016748216298194277	1.0285253755443136
>5k	0.0016748216298194277	3.0363958178075
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC	6746	1.19675495972084	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGT	5182	0.9192979841792756	No Hit
AATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	5174	0.9178787669130782	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	2882	0.5112730201475632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1672	0.2966164086352275	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTT	1239	0.21980127410230074	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	888	0.15753311654789592	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	857	0.15203364964138155	No Hit
AACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTG	778	0.1380188791376836	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	630	0.11176335971303426	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTAT	619	0.10981193597201303	No Hit
AAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	583	0.10342545827412536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7740215827465757E-4	0.0	0.0	0.7715219863364857	0.0
2	1.7740215827465757E-4	0.0	0.0	0.9473275251866714	0.0
3	1.7740215827465757E-4	0.0	0.0	1.3564169021680317	0.0
4	1.7740215827465757E-4	0.0	0.0	3.746733582760768	0.0
5	1.7740215827465757E-4	0.0	0.0	4.013546428805853	0.0
6	1.7740215827465757E-4	0.0	0.0	5.249684667663667	0.0
7	1.7740215827465757E-4	0.0	0.0	6.192399736735197	0.0
8	1.7740215827465757E-4	0.0	0.0	6.693206029544555	0.0
9	1.7740215827465757E-4	0.0	0.0	8.753909500062978	0.0
10	1.7740215827465757E-4	0.0	0.0	9.867640249711277	0.0
11	1.7740215827465757E-4	0.0	0.0	12.51412564685262	0.0
12	1.7740215827465757E-4	0.0	0.0	13.174416479950896	0.0
13	1.7740215827465757E-4	0.0	0.0	13.504384494341759	0.0
14	1.7740215827465757E-4	0.0	0.0	13.903184546143189	0.0
15	1.7740215827465757E-4	0.0	0.0	14.153321589310455	0.0
16	1.7740215827465757E-4	0.0	0.0	14.598423604421571	0.0
17	1.7740215827465757E-4	0.0	0.0	15.189350193634455	0.0
18	1.7740215827465757E-4	0.0	0.0	15.890266120977628	0.0
19	1.7740215827465757E-4	0.0	0.0	16.244006024577295	0.0
20	1.7740215827465757E-4	0.0	0.0	16.564749126737876	0.0
21	1.7740215827465757E-4	0.0	0.0	16.964790993647227	0.0
22	1.7740215827465757E-4	0.0	0.0	17.413441051923837	0.0
23	1.7740215827465757E-4	0.0	0.0	17.871493424589005	0.0
24	1.7740215827465757E-4	0.0	0.0	18.207847916677753	0.0
25	1.7740215827465757E-4	0.0	0.0	18.47927321883798	0.0
26	1.7740215827465757E-4	0.0	0.0	18.75034371668166	0.0
27	1.7740215827465757E-4	0.0	0.0	19.017688769201566	0.0
28	1.7740215827465757E-4	0.0	0.0	19.302419233232392	0.0
29	1.7740215827465757E-4	0.0	0.0	19.61588884690371	0.0
30	1.7740215827465757E-4	0.0	0.0	20.0086572253238	0.0
31	1.7740215827465757E-4	0.0	0.0	20.31627256777206	0.0
32	1.7740215827465757E-4	0.0	0.0	20.611292356982815	0.0
33	1.7740215827465757E-4	0.0	0.0	20.910392395833888	0.0
34	1.7740215827465757E-4	0.0	0.0	21.18589794763443	0.0
35	1.7740215827465757E-4	0.0	0.0	21.54726614403991	0.0
36	1.7740215827465757E-4	0.0	0.0	21.838915292243446	0.0
37	1.7740215827465757E-4	0.0	0.0	22.148482058432723	0.0
38	1.7740215827465757E-4	0.0	0.0	22.467983345485383	0.0
39	1.7740215827465757E-4	0.0	0.0	22.77985633973223	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.0302223E-4	45.000004	17
TCGTTCT	20	7.0302223E-4	45.000004	41
ATTACGG	35	1.2104465E-7	45.000004	2
TTCGTGG	20	7.0302223E-4	45.000004	2
CACGATA	20	7.0302223E-4	45.000004	20
TTCAACG	20	7.0302223E-4	45.000004	1
TGACGTC	20	7.0302223E-4	45.000004	44
ACGATTG	20	7.0302223E-4	45.000004	1
GCCGAAT	20	7.0302223E-4	45.000004	32
TAACACG	20	7.0302223E-4	45.000004	1
CGAATAT	85	0.0	45.000004	13
GCGATAT	20	7.0302223E-4	45.000004	35
ACGTCCA	20	7.0302223E-4	45.000004	35
AATTACG	20	7.0302223E-4	45.000004	1
AACCTCG	20	7.0302223E-4	45.000004	1
TGGATCG	20	7.0302223E-4	45.000004	37
CCGAATT	20	7.0302223E-4	45.000004	33
CCGAACA	20	7.0302223E-4	45.000004	27
TCATACG	35	1.2104465E-7	45.000004	1
GCTACGA	85	0.0	45.000004	9
>>END_MODULE