FastQCFastQC Report
Sat 18 Jun 2016
SRR3548856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423290
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT28180.6657374376904722Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC27170.6418767275390395No Hit
AATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT25460.6014788915400789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24630.5818705851780104No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGT24470.5780906706985754No Hit
AAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT12960.3061730728342271No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT9900.2338822084150346No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA7100.1677337050249238No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.14340050556356163No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT5360.12662713506106923No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.11552363627772921No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT4760.11245245576318837No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT4450.10512887145928325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA351.2095006E-745.00000443
CAACGCG302.16224E-645.0000041
CTATCGA351.2095006E-745.00000418
CGTGAGA302.16224E-645.00000421
CGGTTAG253.8866146E-545.0000041
GGTCCAA253.8866146E-545.00000423
CGACACG302.16224E-645.0000041
TCTAGCG302.16224E-645.0000041
CGTTACG302.16224E-645.0000041
CACTCTA253.8866146E-545.00000443
GCCGATG253.8866146E-545.0000049
CTAACGT302.16224E-645.00000445
CCGTAGG253.8866146E-545.0000042
TCTACGG253.8866146E-545.0000042
CATACCG253.8866146E-545.0000041
CAAGCCG302.16224E-645.0000041
TCCCGCA253.8866146E-545.00000414
TATCGAG351.2095006E-745.00000419
GTAGTGA253.8866146E-545.0000048
CACCACG302.16224E-645.0000041
Produced by FastQC (version 0.11.5)