##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423290
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44487703465709	31.0	31.0	34.0	30.0	34.0
2	31.62616645798389	31.0	31.0	34.0	30.0	34.0
3	31.823409482860452	31.0	31.0	34.0	30.0	34.0
4	35.63585721373054	37.0	35.0	37.0	33.0	37.0
5	35.23737862930851	37.0	35.0	37.0	33.0	37.0
6	35.24763873467363	37.0	35.0	37.0	32.0	37.0
7	35.21373053934655	37.0	35.0	37.0	32.0	37.0
8	35.5431453613362	37.0	35.0	37.0	33.0	37.0
9	36.47800326017624	39.0	37.0	39.0	32.0	39.0
10	36.13218597179239	38.0	35.0	39.0	31.0	39.0
11	36.35377164591651	38.0	35.0	39.0	32.0	39.0
12	35.970107963807315	37.0	35.0	39.0	32.0	39.0
13	35.661418885397715	37.0	35.0	39.0	30.0	39.0
14	36.689191807035364	38.0	35.0	40.0	31.0	41.0
15	36.76763920716294	38.0	35.0	40.0	31.0	41.0
16	36.84598029719577	38.0	35.0	40.0	32.0	41.0
17	36.82224243426492	38.0	35.0	40.0	32.0	41.0
18	36.63087245151078	38.0	35.0	40.0	31.0	41.0
19	36.64679770370196	38.0	35.0	40.0	31.0	41.0
20	36.61286352146283	38.0	35.0	40.0	31.0	41.0
21	36.65347161520471	38.0	35.0	40.0	31.0	41.0
22	36.63022750360273	38.0	35.0	40.0	31.0	41.0
23	36.545101467079306	38.0	35.0	40.0	31.0	41.0
24	36.18924614330601	38.0	35.0	40.0	30.0	41.0
25	36.29111483852678	38.0	35.0	40.0	31.0	41.0
26	36.11328403694866	38.0	35.0	40.0	30.0	41.0
27	36.05492215738619	38.0	34.0	40.0	30.0	41.0
28	35.98166977722129	38.0	35.0	40.0	30.0	41.0
29	35.66577051194217	38.0	34.0	40.0	29.0	41.0
30	35.69077228377708	38.0	34.0	40.0	29.0	41.0
31	35.39076519643743	38.0	34.0	40.0	28.0	41.0
32	35.36367974674573	38.0	34.0	40.0	28.0	41.0
33	35.320307590540764	38.0	34.0	40.0	28.0	41.0
34	35.10593919062581	37.0	34.0	40.0	27.0	41.0
35	35.007727562663895	37.0	34.0	40.0	26.0	41.0
36	34.697755675777834	37.0	33.0	40.0	25.0	41.0
37	34.56706513265137	37.0	33.0	40.0	24.0	41.0
38	34.5029884948853	37.0	33.0	40.0	24.0	41.0
39	34.50370431618985	37.0	33.0	40.0	24.0	41.0
40	34.30351768291242	37.0	33.0	40.0	23.0	41.0
41	34.386567128918706	37.0	33.0	40.0	23.0	41.0
42	34.253098348649864	37.0	33.0	40.0	23.0	41.0
43	34.16809043445392	37.0	33.0	40.0	23.0	41.0
44	34.01092867773867	36.0	33.0	40.0	23.0	41.0
45	33.70432327718586	36.0	33.0	40.0	23.0	41.0
46	33.67619835101231	36.0	33.0	40.0	22.0	41.0
47	33.539112665075955	35.0	32.0	39.0	22.0	41.0
48	33.52236764393206	35.0	32.0	39.0	22.0	41.0
49	33.385040988447635	36.0	32.0	39.0	20.0	41.0
50	33.14263743532803	35.0	32.0	39.0	20.0	40.0
51	31.25598998322663	34.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	2.0
11	8.0
12	8.0
13	7.0
14	15.0
15	36.0
16	92.0
17	195.0
18	409.0
19	768.0
20	1288.0
21	1888.0
22	2656.0
23	3572.0
24	4381.0
25	5034.0
26	5654.0
27	6639.0
28	7823.0
29	9521.0
30	11806.0
31	14808.0
32	18787.0
33	25788.0
34	42733.0
35	45510.0
36	39514.0
37	53427.0
38	69244.0
39	51671.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.160646365375985	35.16147322166836	19.984644097427296	24.69323631552836
2	22.04729617992393	34.74591887358549	20.154740249001865	23.052044697488718
3	20.38248009638782	31.882161166103618	21.723641002622315	26.011717734886247
4	21.046091332183607	32.832809657681494	18.958161071605755	27.16293793852914
5	18.25651444636065	36.53452715632309	17.685274870656052	27.523683526660207
6	20.79283706206147	36.74478489924166	20.829927472890926	21.63245056580595
7	79.29339223700063	9.464905856504997	6.39986770299322	4.841834203501145
8	81.46660681802074	6.517990030475561	5.0010631009473405	7.014340050556356
9	75.32967941600322	10.92088166505233	8.827045288100356	4.922393630844102
10	40.86630914975549	32.18904297290274	11.950908360698339	14.993739516643437
11	33.58902879822344	25.452999125894777	24.027262633182925	16.93070944269886
12	33.215053509414346	24.504713080866544	25.80027876869286	16.479954641026247
13	23.20702119114555	35.37102223062203	22.909589170545015	18.512367407687403
14	17.316024474946254	34.68047910416027	29.73918590091899	18.264310519974487
15	16.262373313803774	29.481442982352522	33.637695197146165	20.618488506697535
16	17.41666469796121	25.920763542724846	34.31217368707033	22.350398072243614
17	17.428240686054476	26.887004181530394	28.023577216565478	27.661177915849656
18	21.205792718939733	27.15136195043587	27.32193059132037	24.320914739304023
19	22.899430650381536	31.127123248836497	25.95218408183515	20.02126201894682
20	26.40790002126202	29.4167119468922	24.109003283800703	20.066384748045074
21	20.99270004016159	30.84788206666824	24.983580996479954	23.175836896690214
22	21.044201374943892	28.40416735571358	25.76956696354745	24.782064305795082
23	21.78742705946278	30.239788324789153	21.86515155094616	26.10763306480191
24	17.9137234520069	30.637388079094713	28.94469512627277	22.504193342625623
25	20.1625363226157	28.54355170214274	27.190106073850078	24.103805901391482
26	22.331734744501404	32.54388244465969	23.81464244371471	21.309740367124196
27	19.498688842164945	30.826856292376387	25.969666186302536	23.704788679156135
28	21.742304330364526	28.566467433674315	29.827777646530745	19.863450589430414
29	20.037562900139385	28.335892650428786	30.726688558671363	20.89985589076047
30	24.775685700111037	27.77150416971816	25.13170639514281	22.321103735027993
31	28.160835361099956	27.59644688038933	23.639821399040848	20.602896359469867
32	26.44688038933119	26.070778898627417	26.196224810413664	21.286115901627724
33	25.42039736350965	25.84729145503083	24.791750336648633	23.94056084481089
34	22.14533771173427	27.03229464433367	28.153275532141087	22.66909211179097
35	23.795270382007608	24.226653121973115	29.739894634883886	22.23818186113539
36	28.059486404120104	24.174206808570954	29.543575326608234	18.222731460700704
37	23.343570601715136	27.5484892154315	27.648656949136523	21.459283233716835
38	23.32656098655768	27.102695551513143	28.044130501547404	21.526612960381772
39	22.71893973398852	24.790332868718846	29.041791679463252	23.448935717829382
40	25.260223487443596	24.349736587209712	25.39417420680857	24.99586571853812
41	22.283304590233648	21.41463299392851	30.93293014245553	25.369132273382316
42	26.05943915518911	24.494554560703065	25.80004252403789	23.64596376006993
43	23.6535235890288	23.662973375227388	28.13130477922937	24.552198256514444
44	23.43405230456661	24.378558435115405	26.52956601856883	25.657823241749156
45	24.30461385811146	27.909234803562573	25.544425807366107	22.24172553095986
46	21.59914006945593	26.472867301377306	29.938103900399256	21.98988872876751
47	22.363155283611707	25.98667580145999	26.704623307897656	24.945545607030642
48	24.3712348508115	22.41418412908408	31.793333175836896	21.421247844267523
49	22.469937867655744	21.22421980202698	30.932221408490634	25.373620921826646
50	20.86134801200123	25.40409648231709	26.442864230196793	27.29169127548489
51	20.485719010607383	24.969406317182074	29.368518037279408	25.176356634931135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2884.0
1	2330.0
2	1776.0
3	1243.5
4	711.0
5	700.5
6	690.0
7	706.5
8	723.0
9	798.5
10	874.0
11	904.5
12	935.0
13	1026.0
14	1117.0
15	1162.0
16	1207.0
17	1208.0
18	1209.0
19	1271.5
20	1334.0
21	1304.5
22	1275.0
23	1442.5
24	1610.0
25	1930.5
26	2090.5
27	1930.0
28	2658.5
29	3387.0
30	4254.0
31	5121.0
32	6197.5
33	7274.0
34	7894.0
35	8514.0
36	9842.5
37	11171.0
38	12090.0
39	13009.0
40	15989.5
41	18970.0
42	23559.5
43	28149.0
44	36058.0
45	43967.0
46	43693.0
47	43419.0
48	43047.5
49	42676.0
50	39800.5
51	36925.0
52	34475.0
53	32025.0
54	27738.0
55	23451.0
56	20936.5
57	18422.0
58	16780.0
59	15138.0
60	14558.0
61	13978.0
62	12079.0
63	10180.0
64	9265.5
65	8351.0
66	7191.5
67	6032.0
68	5052.0
69	4072.0
70	3481.0
71	2890.0
72	2520.0
73	2150.0
74	1772.0
75	1171.0
76	948.0
77	735.0
78	522.0
79	396.0
80	270.0
81	207.5
82	145.0
83	117.0
84	89.0
85	80.5
86	72.0
87	44.5
88	17.0
89	19.0
90	21.0
91	12.5
92	4.0
93	4.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.430033085781773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70133905402977	24.557444174810186
2	8.255102048729231	5.024060569386708
3	2.6460864181911683	2.4156149176018515
4	1.178718743367445	1.4347380143800987
5	0.7072246812002821	1.0760435224003027
6	0.4686535575098408	0.8556685956476271
7	0.32292838991539535	0.6878705112624602
8	0.27334465944229225	0.6654309624520564
9	0.23307359813807804	0.6383193572487529
>10	3.954476863946177	31.52339560741599
>50	1.1707789680946186	23.07592074361527
>100	0.08052977029222246	4.009197944050215
>500	0.0030972988573931713	0.6698883194202034
>1k	0.0046459482860897565	3.3664067603082826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	2818	0.6657374376904722	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC	2717	0.6418767275390395	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	2546	0.6014788915400789	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2463	0.5818705851780104	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGT	2447	0.5780906706985754	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	1296	0.3061730728342271	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT	990	0.2338822084150346	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	710	0.1677337050249238	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	607	0.14340050556356163	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT	536	0.12662713506106923	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	489	0.11552363627772921	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	476	0.11245245576318837	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	445	0.10512887145928325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3624465496468143E-4	0.0	0.0	1.0238843346169293	0.0
2	2.3624465496468143E-4	0.0	0.0	1.2955656878263129	0.0
3	2.3624465496468143E-4	0.0	0.0	1.696709111956342	0.0
4	2.3624465496468143E-4	0.0	0.0	4.108767039145739	0.0
5	2.3624465496468143E-4	0.0	0.0	4.436910864891682	0.0
6	2.3624465496468143E-4	0.0	0.0	5.996834321623473	0.0
7	2.3624465496468143E-4	0.0	0.0	7.176403883862128	0.0
8	2.3624465496468143E-4	0.0	0.0	7.728035153204659	0.0
9	2.3624465496468143E-4	0.0	0.0	9.3893075669163	0.0
10	2.3624465496468143E-4	0.0	0.0	10.925370313496657	0.0
11	2.3624465496468143E-4	0.0	0.0	13.060077015757518	0.0
12	2.3624465496468143E-4	0.0	0.0	13.76006992841787	0.0
13	2.3624465496468143E-4	0.0	0.0	14.062226842117697	0.0
14	2.3624465496468143E-4	0.0	0.0	14.426043610763307	0.0
15	2.3624465496468143E-4	0.0	0.0	14.692291336908502	0.0
16	2.3624465496468143E-4	0.0	0.0	15.19076755888398	0.0
17	2.3624465496468143E-4	0.0	0.0	15.79626260955846	0.0
18	2.3624465496468143E-4	0.0	0.0	16.57303503508233	0.0
19	2.3624465496468143E-4	0.0	0.0	16.9136998275414	0.0
20	2.3624465496468143E-4	0.0	0.0	17.216565475206124	0.0
21	2.3624465496468143E-4	0.0	0.0	17.5959743910794	0.0
22	2.3624465496468143E-4	0.0	0.0	17.983651869876443	0.0
23	2.3624465496468143E-4	0.0	0.0	18.39873372864939	0.0
24	2.3624465496468143E-4	0.0	0.0	18.70254435493397	0.0
25	2.3624465496468143E-4	0.0	0.0	18.94894753006213	0.0
26	2.3624465496468143E-4	0.0	0.0	19.17692362210305	0.0
27	2.3624465496468143E-4	0.0	0.0	19.422381818611353	0.0
28	2.3624465496468143E-4	0.0	0.0	19.669257483049446	0.0
29	2.3624465496468143E-4	0.0	0.0	19.9284178695457	0.0
30	2.3624465496468143E-4	0.0	0.0	20.28703725578209	0.0
31	2.3624465496468143E-4	0.0	0.0	20.544780174348556	0.0
32	2.3624465496468143E-4	0.0	0.0	20.790002126201895	0.0
33	2.3624465496468143E-4	0.0	0.0	21.04254766235914	0.0
34	2.3624465496468143E-4	0.0	0.0	21.28068227456354	0.0
35	2.3624465496468143E-4	0.0	0.0	21.592997708426847	0.0
36	2.3624465496468143E-4	0.0	0.0	21.833967256490823	0.0
37	2.3624465496468143E-4	0.0	0.0	22.082260388858703	0.0
38	2.3624465496468143E-4	0.0	0.0	22.32984478726169	0.0
39	2.3624465496468143E-4	0.0	0.0	22.589005173757943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTA	35	1.2095006E-7	45.000004	43
CAACGCG	30	2.16224E-6	45.000004	1
CTATCGA	35	1.2095006E-7	45.000004	18
CGTGAGA	30	2.16224E-6	45.000004	21
CGGTTAG	25	3.8866146E-5	45.000004	1
GGTCCAA	25	3.8866146E-5	45.000004	23
CGACACG	30	2.16224E-6	45.000004	1
TCTAGCG	30	2.16224E-6	45.000004	1
CGTTACG	30	2.16224E-6	45.000004	1
CACTCTA	25	3.8866146E-5	45.000004	43
GCCGATG	25	3.8866146E-5	45.000004	9
CTAACGT	30	2.16224E-6	45.000004	45
CCGTAGG	25	3.8866146E-5	45.000004	2
TCTACGG	25	3.8866146E-5	45.000004	2
CATACCG	25	3.8866146E-5	45.000004	1
CAAGCCG	30	2.16224E-6	45.000004	1
TCCCGCA	25	3.8866146E-5	45.000004	14
TATCGAG	35	1.2095006E-7	45.000004	19
GTAGTGA	25	3.8866146E-5	45.000004	8
CACCACG	30	2.16224E-6	45.000004	1
>>END_MODULE