SRR3548854_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	6843	1.6592349043084824	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6723	1.6301382816989518	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	6374	1.5455156042762337	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5745	1.3930008074312774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4201	1.0186242631886504	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3420	0.8292537443716221	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2819	0.6835281594688897	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1584	0.3840754184458039	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1557	0.3775286783586595	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1272	0.3084241996610243	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1083	0.2625970190510136	TruSeq Adapter, Index 19 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	991	0.24028960838370686	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	966	0.23422781200672133	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.1847635535705193	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	741	0.17967164461385143	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	736	0.17845928533845434	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	716	0.1736098482368659	TruSeq Adapter, Index 19 (95% over 22bp)
CAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	650	0.15760670580162406	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	599	0.14524064119257357	No Hit
TAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	537	0.13020738617764943	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	534	0.12947997061241115	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	498	0.12075098382955199	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	497	0.12050851197447257	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	479	0.11614401858304298	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	464	0.11250694075685165	No Hit
AAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	461	0.11177952519161338	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	436	0.10571772881462783	No Hit
CAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	435	0.10547525695954842	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	424	0.10280806655367479	TruSeq Adapter, Index 19 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGAGGG	45	3.8380676E-10	45.000004	3
CGATAGG	45	3.8380676E-10	45.000004	2
ATACAGA	45	3.8380676E-10	45.000004	24
CTAGATA	20	7.0280215E-4	45.0	20
CGGGTTG	20	7.0280215E-4	45.0	40
TATCACG	40	6.7975634E-9	45.0	1
GTGCACA	35	1.2093915E-7	45.0	21
TGTCAGA	20	7.0280215E-4	45.0	36
AGACTCG	20	7.0280215E-4	45.0	35
CTATGAA	20	7.0280215E-4	45.0	43
TAGAGAT	20	7.0280215E-4	45.0	13
CGGAAAT	20	7.0280215E-4	45.0	6
ACTATCC	20	7.0280215E-4	45.0	24
GGCAGAC	25	3.8864408E-5	45.0	20
GTACCGG	25	3.8864408E-5	45.0	2
GGTACCC	20	7.0280215E-4	45.0	9
GCAAGAC	70	0.0	45.0	45
CATATGT	20	7.0280215E-4	45.0	25
TCACCCC	20	7.0280215E-4	45.0	22
ATCTAGC	25	3.8864408E-5	45.0	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content