##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05102092774581	31.0	31.0	34.0	28.0	34.0
2	31.2123350282116	31.0	31.0	34.0	28.0	34.0
3	31.43276376694575	31.0	31.0	34.0	30.0	34.0
4	35.31145752256807	37.0	35.0	37.0	33.0	37.0
5	34.81768056272868	35.0	35.0	37.0	32.0	37.0
6	34.80501383301933	35.0	35.0	37.0	32.0	37.0
7	34.7032386965683	35.0	35.0	37.0	32.0	37.0
8	35.06200732749946	35.0	35.0	37.0	32.0	37.0
9	35.95702428840573	39.0	35.0	39.0	32.0	39.0
10	35.74212875740449	37.0	35.0	39.0	30.0	39.0
11	36.04811369020341	37.0	35.0	39.0	31.0	39.0
12	35.549332111275184	37.0	35.0	39.0	30.0	39.0
13	35.19545898709807	37.0	34.0	39.0	30.0	39.0
14	36.070784808653336	38.0	34.0	40.0	30.0	41.0
15	36.17883026727672	38.0	35.0	40.0	31.0	41.0
16	36.19597545214939	38.0	34.0	40.0	31.0	41.0
17	36.10813274849122	38.0	34.0	40.0	31.0	41.0
18	35.855692875449485	37.0	34.0	40.0	30.0	41.0
19	35.75155848784852	37.0	34.0	40.0	29.0	41.0
20	35.74846454697771	37.0	34.0	40.0	30.0	41.0
21	35.83036184074933	37.0	34.0	40.0	30.0	41.0
22	35.77475577022397	37.0	34.0	40.0	30.0	41.0
23	35.687844158489305	37.0	34.0	40.0	30.0	41.0
24	35.245798568931114	37.0	34.0	40.0	28.0	41.0
25	35.3365921550656	37.0	34.0	40.0	29.0	41.0
26	35.1712724195539	37.0	34.0	40.0	28.0	41.0
27	35.06454115838504	37.0	34.0	40.0	27.0	41.0
28	35.113195560825275	37.0	34.0	40.0	27.0	41.0
29	34.704642608609205	36.0	33.0	39.0	27.0	41.0
30	34.6914909351897	36.0	33.0	39.0	26.0	41.0
31	34.45009323042828	36.0	33.0	39.0	25.0	40.0
32	34.07986780434461	36.0	33.0	39.0	24.0	40.0
33	34.24489172419312	36.0	33.0	39.0	25.0	40.0
34	34.00616848399322	36.0	33.0	39.0	24.0	40.0
35	33.97503994723812	36.0	33.0	39.0	24.0	40.0
36	33.76708153601071	36.0	33.0	39.0	23.0	40.0
37	33.4970236579789	35.0	33.0	39.0	22.0	40.0
38	33.32386480739248	35.0	32.0	39.0	21.0	40.0
39	33.06618754228103	35.0	32.0	39.0	20.0	40.0
40	32.851182898945005	35.0	31.0	39.0	18.0	40.0
41	32.89042454397106	35.0	31.0	39.0	18.0	40.0
42	32.7198843894195	35.0	31.0	39.0	16.0	40.0
43	32.463009706148355	35.0	31.0	39.0	15.0	40.0
44	32.38550115295367	35.0	31.0	39.0	15.0	40.0
45	31.752581719076957	35.0	30.0	39.0	15.0	40.0
46	31.625999287132746	35.0	30.0	38.0	14.0	40.0
47	31.447741253434007	35.0	30.0	38.0	12.0	40.0
48	31.383689888196226	35.0	30.0	38.0	12.0	40.0
49	31.33472269706294	35.0	30.0	38.0	10.0	40.0
50	30.9013915459763	35.0	28.0	38.0	10.0	40.0
51	28.83071100022065	33.0	23.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	3.0
13	4.0
14	21.0
15	36.0
16	96.0
17	226.0
18	531.0
19	1054.0
20	1793.0
21	2840.0
22	3736.0
23	4846.0
24	5970.0
25	6545.0
26	7572.0
27	8900.0
28	10661.0
29	12700.0
30	15697.0
31	19231.0
32	23606.0
33	30936.0
34	46967.0
35	50686.0
36	39938.0
37	47141.0
38	46982.0
39	23691.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.011097936806983	40.87129836404239	18.67663710934753	23.44096658980309
2	16.88283032546997	43.74992422754529	19.280392028495292	20.086853418489447
3	17.59642499496871	35.462236220930656	25.608180030502957	21.333158753597676
4	20.453228391514454	34.464222065423755	18.713492831319606	26.369056711742182
5	14.400645945021932	41.646723356586385	20.11134307585247	23.841287622539216
6	19.93530850906481	36.39163084145008	23.14684822959175	20.526212419893362
7	63.08972186053504	15.830502474425282	12.324116978121765	8.755658686917917
8	65.97513693598016	10.311358109107486	9.23211588214898	14.48138907276338
9	61.65234870362423	13.909397966631024	16.954359522718402	7.48389380702635
10	37.537310356700345	27.374829966611625	15.32373629730929	19.764123379378738
11	30.561395086065385	22.690758670187357	28.81002087682672	17.937825366920535
12	28.22081426898373	23.052284206110777	31.95269859051111	16.77420293439439
13	20.063576120401823	37.23252323486551	23.70865551781077	18.99524512692189
14	11.546509738882058	36.80165074838938	36.79655883943271	14.855280673295848
15	9.416879435719498	32.193958086315135	36.309190410723076	22.079972067242295
16	10.040517046983771	27.33918660391495	38.65462066490632	23.965675684194956
17	9.308979460209157	30.525994195223788	30.877093441378793	29.287932903188263
18	14.046879508461055	29.408198943307656	28.600525194038102	27.944396354193184
19	16.143776111187893	35.798059740215656	29.655762707343747	18.402401441252707
20	19.509527931545346	34.712513245025086	25.029642184283457	20.74831663914611
21	13.131790727391317	35.559467434817506	24.97872309471678	26.330018743074397
22	12.639572861580092	34.76197750346128	27.44417691716434	25.154272717794278
23	19.200133844464006	31.437688370322412	19.681682948651734	29.680494836561845
24	12.21282239664031	37.64642269148609	26.48447331475999	23.656281597113615
25	14.103860394404718	28.77655976082576	29.80851997604378	27.31105986872574
26	18.864067853323927	36.35332028834753	21.708020241550464	23.074591616778083
27	11.64277106534859	32.83214400888417	27.71647281041853	27.808612115348712
28	15.905668749499903	27.524920044905787	35.145083034486774	21.42432817110754
29	14.9760316571254	30.90594759213324	27.257231116898105	26.86078963384325
30	16.716737104740567	28.141283500517677	30.505869031252196	24.636110363489557
31	20.267494950523616	29.57647441073277	24.45983332484682	25.69619731389679
32	19.834925161061932	29.96467185071493	21.983225797065607	28.21717719115754
33	19.79273505827811	23.86407997691668	28.618468111313977	27.724716853491234
34	12.638845446014853	26.60886137641573	25.515313310007542	35.23697986756187
35	13.86866269497768	23.70865551781077	29.60266137108135	32.8200204161302
36	17.819741573496856	20.679454632303557	33.24507357808442	28.255730216115165
37	14.389249767833197	28.41988366200393	26.409549511540447	30.78131705862242
38	19.076958142083658	25.936971865990653	28.880580186654836	26.105489805270853
39	15.757033502336215	25.79803549303015	28.808081101986087	29.63684990264755
40	18.692397779927695	24.843423799582464	20.679212160448476	35.784966260041365
41	15.428484138703599	18.19120845547853	31.865408722682513	34.51489868313536
42	23.43660209641166	21.242716751653052	24.67611821957766	30.644562932357626
43	18.705491260101983	20.914652331730593	30.22872370089642	30.151132707271007
44	19.54153421641583	21.78464134775556	27.352765027799396	31.32105940802921
45	24.192144396839137	25.660311479345033	25.92436332952652	24.223180794289302
46	17.05062084918493	22.37069582148252	35.56092226594798	25.01776106338457
47	20.232821475247263	22.76131798001547	24.81190245842214	32.193958086315135
48	19.97652872442831	17.116330721911453	37.73807705270611	25.169063500954124
49	21.00557927738538	15.740545416190816	32.81856558499972	30.435309721424087
50	18.08161117698263	19.74327079984191	26.207085512549135	35.96803251062633
51	15.777158666307809	20.244945068001233	35.97021475732205	28.007681508368915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4961.0
1	4033.0
2	3105.0
3	2340.5
4	1576.0
5	1579.0
6	1582.0
7	1701.5
8	1821.0
9	2018.5
10	2216.0
11	2396.0
12	2576.0
13	2716.5
14	2857.0
15	2779.5
16	2702.0
17	2670.0
18	2638.0
19	2455.5
20	2273.0
21	2017.5
22	1762.0
23	1771.0
24	1780.0
25	1850.5
26	2230.0
27	2539.0
28	2645.0
29	2751.0
30	3071.0
31	3391.0
32	3408.0
33	3425.0
34	4266.5
35	5108.0
36	5198.0
37	5288.0
38	6849.0
39	8410.0
40	10890.0
41	13370.0
42	21505.5
43	29641.0
44	44361.5
45	59082.0
46	58658.0
47	58234.0
48	56293.5
49	54353.0
50	47687.5
51	41022.0
52	35059.5
53	29097.0
54	23659.5
55	18222.0
56	15213.5
57	12205.0
58	10048.5
59	7892.0
60	7707.5
61	7523.0
62	6612.0
63	5701.0
64	4703.0
65	3705.0
66	2858.5
67	2012.0
68	1753.5
69	1495.0
70	1431.0
71	1367.0
72	1349.0
73	1331.0
74	1021.0
75	542.0
76	373.0
77	270.0
78	167.0
79	142.0
80	117.0
81	73.0
82	29.0
83	27.5
84	26.0
85	30.0
86	34.0
87	23.5
88	13.0
89	8.5
90	4.0
91	7.0
92	10.0
93	5.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	412419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.37814007856402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84296137991781	29.04538433366028
2	9.263652773513403	6.739889164680974
3	3.5191619444141016	3.8406169851914385
4	1.6823709732941223	2.4480610772241467
5	0.9999627011133742	1.8188391607220786
6	0.6577691364226088	1.4357050670482652
7	0.4634875202939415	1.1802569756543502
8	0.3517880392619076	1.0237915656186445
9	0.2724014592394095	0.8918512597634838
>10	2.218663599065616	17.909826076449782
>50	0.599936145096813	15.227536460524977
>100	0.1138613549517814	6.604775895476569
>500	0.006658558769110022	1.7517783135690228
>1k	0.004660991138377016	3.8601132750326257
>5k	0.002663423507644009	6.2215743893833455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	6843	1.6592349043084824	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6723	1.6301382816989518	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	6374	1.5455156042762337	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5745	1.3930008074312774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4201	1.0186242631886504	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3420	0.8292537443716221	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2819	0.6835281594688897	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1584	0.3840754184458039	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1557	0.3775286783586595	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1272	0.3084241996610243	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1083	0.2625970190510136	TruSeq Adapter, Index 19 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	991	0.24028960838370686	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	966	0.23422781200672133	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.1847635535705193	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	741	0.17967164461385143	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	736	0.17845928533845434	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	716	0.1736098482368659	TruSeq Adapter, Index 19 (95% over 22bp)
CAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	650	0.15760670580162406	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	599	0.14524064119257357	No Hit
TAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	537	0.13020738617764943	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	534	0.12947997061241115	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	498	0.12075098382955199	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	497	0.12050851197447257	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	479	0.11614401858304298	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	464	0.11250694075685165	No Hit
AAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	461	0.11177952519161338	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	436	0.10571772881462783	No Hit
CAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	435	0.10547525695954842	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	424	0.10280806655367479	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4247185507942165E-4	0.0	0.0	2.3141513848780004	0.0
2	2.4247185507942165E-4	0.0	0.0	2.8854150754451178	0.0
3	2.4247185507942165E-4	0.0	0.0	3.9134957409818654	0.0
4	2.4247185507942165E-4	0.0	0.0	9.921705837994855	0.0
5	2.4247185507942165E-4	0.0	0.0	10.818124286223476	0.0
6	2.4247185507942165E-4	0.0	0.0	14.86740426604982	0.0
7	2.4247185507942165E-4	0.0	0.0	18.18708643394218	0.0
8	2.4247185507942165E-4	0.0	0.0	20.034479497792294	0.0
9	2.4247185507942165E-4	0.0	0.0	23.97658691767353	0.0
10	2.4247185507942165E-4	0.0	0.0	27.3280328985813	0.0
11	2.4247185507942165E-4	0.0	0.0	32.44831106229344	0.0
12	2.4247185507942165E-4	0.0	0.0	34.31534434640499	0.0
13	2.4247185507942165E-4	0.0	0.0	35.14217337222582	0.0
14	2.4247185507942165E-4	0.0	0.0	35.958091164568074	0.0
15	2.4247185507942165E-4	0.0	0.0	36.494923851713914	0.0
16	2.4247185507942165E-4	0.0	0.0	37.43741195240762	0.0
17	2.4247185507942165E-4	0.0	0.0	38.47276677359675	0.0
18	2.4247185507942165E-4	0.0	0.0	40.001794291727585	0.0
19	2.4247185507942165E-4	0.0	0.0	40.58275685649788	2.4247185507942165E-4
20	2.4247185507942165E-4	0.0	0.0	41.11498257839721	2.4247185507942165E-4
21	2.4247185507942165E-4	0.0	0.0	41.66248402716655	2.4247185507942165E-4
22	2.4247185507942165E-4	0.0	0.0	42.20513603883429	2.4247185507942165E-4
23	2.4247185507942165E-4	0.0	0.0	42.7470606349368	2.4247185507942165E-4
24	2.4247185507942165E-4	0.0	0.0	43.09549269068593	2.4247185507942165E-4
25	2.4247185507942165E-4	0.0	0.0	43.37215307733155	2.4247185507942165E-4
26	2.4247185507942165E-4	0.0	0.0	43.64954087954241	2.4247185507942165E-4
27	2.4247185507942165E-4	0.0	0.0	43.9890014766536	2.4247185507942165E-4
28	2.4247185507942165E-4	0.0	0.0	44.330159376750345	2.4247185507942165E-4
29	2.4247185507942165E-4	0.0	0.0	44.67252963612249	2.4247185507942165E-4
30	2.4247185507942165E-4	0.0	0.0	45.21639400706563	2.4247185507942165E-4
31	2.4247185507942165E-4	0.0	0.0	45.56967549991635	2.4247185507942165E-4
32	2.4247185507942165E-4	0.0	0.0	45.84415363986625	2.4247185507942165E-4
33	2.4247185507942165E-4	0.0	0.0	46.10772054633758	2.4247185507942165E-4
34	2.4247185507942165E-4	0.0	0.0	46.367892846837805	2.4247185507942165E-4
35	2.4247185507942165E-4	0.0	0.0	46.69353254820947	2.4247185507942165E-4
36	2.4247185507942165E-4	0.0	0.0	47.00050191674001	2.4247185507942165E-4
37	2.4247185507942165E-4	0.0	0.0	47.27570747225516	2.4247185507942165E-4
38	2.4247185507942165E-4	0.0	0.0	47.52084651774045	2.4247185507942165E-4
39	2.4247185507942165E-4	0.0	0.0	47.766955450646066	2.4247185507942165E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGAGGG	45	3.8380676E-10	45.000004	3
CGATAGG	45	3.8380676E-10	45.000004	2
ATACAGA	45	3.8380676E-10	45.000004	24
CTAGATA	20	7.0280215E-4	45.0	20
CGGGTTG	20	7.0280215E-4	45.0	40
TATCACG	40	6.7975634E-9	45.0	1
GTGCACA	35	1.2093915E-7	45.0	21
TGTCAGA	20	7.0280215E-4	45.0	36
AGACTCG	20	7.0280215E-4	45.0	35
CTATGAA	20	7.0280215E-4	45.0	43
TAGAGAT	20	7.0280215E-4	45.0	13
CGGAAAT	20	7.0280215E-4	45.0	6
ACTATCC	20	7.0280215E-4	45.0	24
GGCAGAC	25	3.8864408E-5	45.0	20
GTACCGG	25	3.8864408E-5	45.0	2
GGTACCC	20	7.0280215E-4	45.0	9
GCAAGAC	70	0.0	45.0	45
CATATGT	20	7.0280215E-4	45.0	25
TCACCCC	20	7.0280215E-4	45.0	22
ATCTAGC	25	3.8864408E-5	45.0	42
>>END_MODULE