SRR3548853_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410603
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	6579	1.6022776258332256	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6457	1.5725652272389632	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	6016	1.465162212648227	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5431	1.3226888259462304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4321	1.0523547075885953	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3272	0.7968767885280916	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2712	0.6604920080954109	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1514	0.3687259956697832	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1359	0.33097663680002337	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1197	0.291522468174855	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1073	0.26132298107904717	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1019	0.24817159153732435	TruSeq Adapter, Index 19 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	945	0.2301493169801487	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.19556603337043324	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	739	0.17997920132098402	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	717	0.17462122780398584	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	688	0.16755844453157914	TruSeq Adapter, Index 19 (95% over 22bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	600	0.14612655046358647	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	565	0.13760250168654392	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	554	0.13492351492804486	No Hit
TAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	547	0.13321870517263634	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	528	0.1285913644079561	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	505	0.12298984664018528	No Hit
CAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	437	0.10642883758764547	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	436	0.10618529333687285	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGC	30	2.16208E-6	45.000004	39
GCAAGAC	65	0.0	45.000004	45
GTCCTCG	30	2.16208E-6	45.000004	22
TATGGGT	35	1.2093733E-7	45.000004	25
GGGTATA	30	2.16208E-6	45.000004	7
GATCAGT	30	2.16208E-6	45.000004	40
ACTTATA	35	1.2093733E-7	45.000004	9
CGACAGG	30	2.16208E-6	45.000004	2
TCGATCA	30	2.16208E-6	45.000004	17
TAGCCGT	35	1.2093733E-7	45.000004	44
GCCGCGA	30	2.16208E-6	45.000004	26
TTATAGG	35	1.2093733E-7	45.000004	2
TATTGCG	30	2.16208E-6	45.000004	1
TGGTTAT	30	2.16208E-6	45.000004	17
AATGCGG	60	0.0	45.000004	2
GTCACTT	35	1.2093733E-7	45.000004	6
TGCGAAG	30	2.16208E-6	45.000004	1
GTCACGG	35	1.2093733E-7	45.000004	2
ACTACGG	60	0.0	45.000004	2
GATATTG	30	2.16208E-6	45.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content