##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410603
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85996449124824	31.0	30.0	33.0	28.0	34.0
2	31.035640265658067	31.0	31.0	34.0	28.0	34.0
3	31.066904041129753	31.0	31.0	34.0	28.0	34.0
4	35.11286814757808	35.0	35.0	37.0	32.0	37.0
5	34.68902565251594	35.0	35.0	37.0	32.0	37.0
6	34.71726217295051	35.0	35.0	37.0	32.0	37.0
7	34.55017620426543	35.0	35.0	37.0	31.0	37.0
8	34.945448523269434	35.0	35.0	37.0	32.0	37.0
9	35.464957635477575	38.0	35.0	39.0	31.0	39.0
10	35.41958047067362	37.0	35.0	39.0	30.0	39.0
11	35.8718664987835	37.0	35.0	39.0	31.0	39.0
12	35.417067094005645	37.0	35.0	39.0	30.0	39.0
13	35.04083506452705	37.0	34.0	39.0	30.0	39.0
14	35.892355876601	38.0	34.0	40.0	30.0	41.0
15	36.056266028256005	38.0	34.0	40.0	30.0	41.0
16	36.07340910806789	38.0	34.0	40.0	31.0	41.0
17	36.003209913225184	37.0	34.0	40.0	30.0	41.0
18	35.819012525480815	37.0	34.0	40.0	30.0	41.0
19	35.6934898186326	37.0	34.0	40.0	29.0	41.0
20	35.645202300031904	37.0	34.0	40.0	30.0	41.0
21	35.686037364558956	37.0	34.0	40.0	30.0	41.0
22	35.67154648163798	37.0	34.0	40.0	30.0	41.0
23	35.58601374076663	37.0	34.0	40.0	30.0	41.0
24	35.10249316249516	36.0	34.0	39.0	27.0	41.0
25	35.22270660467654	36.0	34.0	39.0	28.0	41.0
26	35.07422498130798	36.0	34.0	39.0	27.0	41.0
27	34.94916500853623	36.0	33.0	39.0	27.0	41.0
28	34.94587229026578	36.0	33.0	39.0	27.0	41.0
29	34.587794049239776	36.0	33.0	39.0	25.0	40.0
30	34.62106219389532	36.0	33.0	39.0	26.0	40.0
31	34.45597085262407	36.0	33.0	39.0	25.0	40.0
32	34.11111462897251	36.0	33.0	39.0	24.0	40.0
33	34.15393457914336	36.0	33.0	39.0	24.0	40.0
34	34.04153160108426	36.0	33.0	39.0	24.0	40.0
35	33.9567830727004	36.0	33.0	39.0	24.0	40.0
36	33.808250305039174	36.0	33.0	39.0	23.0	40.0
37	33.53227570183365	35.0	33.0	39.0	23.0	40.0
38	33.40315341095901	35.0	32.0	39.0	22.0	40.0
39	33.06480956057311	35.0	32.0	39.0	20.0	40.0
40	32.844297289596035	35.0	31.0	39.0	18.0	40.0
41	32.896890670550384	35.0	31.0	39.0	18.0	40.0
42	32.81177439034786	35.0	31.0	39.0	18.0	40.0
43	32.622847373253485	35.0	31.0	39.0	17.0	40.0
44	32.648129701926194	35.0	31.0	39.0	17.0	40.0
45	32.125269420827415	35.0	30.0	39.0	15.0	40.0
46	31.86656210500167	35.0	30.0	38.0	15.0	40.0
47	31.666604968789805	35.0	30.0	38.0	14.0	40.0
48	31.51197141764673	35.0	30.0	38.0	12.0	40.0
49	31.48565159046573	35.0	30.0	38.0	12.0	40.0
50	31.23825690508837	35.0	29.0	38.0	10.0	40.0
51	29.26970577419064	34.0	24.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	8.0
12	4.0
13	11.0
14	17.0
15	48.0
16	112.0
17	272.0
18	594.0
19	1112.0
20	1873.0
21	2821.0
22	4026.0
23	4936.0
24	5754.0
25	6410.0
26	7410.0
27	8586.0
28	10378.0
29	12524.0
30	15447.0
31	19131.0
32	24164.0
33	31891.0
34	48611.0
35	49794.0
36	40393.0
37	47032.0
38	45641.0
39	21596.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.278003326814463	40.387186649878345	18.869808549864466	23.46500147344272
2	16.97966161961798	43.393496881415864	19.385148184499382	20.241693314466772
3	17.98963962757213	35.30685357876099	25.371952956992523	21.331553836674356
4	20.509591990316682	34.6906866243062	18.576824816184978	26.222896569192137
5	14.612167957857103	41.82775089319854	19.8176827738716	23.74239837507276
6	19.820848849131643	36.69968314892974	22.819852753145984	20.65961524879263
7	63.9008969734756	15.908066916218342	11.751253644030852	8.43978246627521
8	66.8994137889884	10.477273668239151	8.785615302372364	13.837697240400097
9	61.96569435683617	14.995750152824016	15.922192482763156	7.116363007576662
10	38.52115060045835	27.46545933663417	14.820398292267715	19.192991770639768
11	31.52534199701415	22.907528683424136	28.037057693197564	17.53007162636415
12	29.163206308770274	23.212446085391488	31.04994361950595	16.5744039863323
13	20.2183130663926	37.865285933127616	23.395591361972514	18.52080963850727
14	11.85524703911077	37.48803588868079	35.88697598410143	14.769741088107002
15	9.519657674201113	32.12981882743185	36.77104161440613	21.57948188396091
16	10.304357250190574	27.198778382038125	38.88451862261114	23.61234574516017
17	9.449760474229366	30.190232414278512	30.75549862032182	29.6045084911703
18	14.40539888895113	29.365835125413113	28.74138766643205	27.48737831920371
19	16.754139643402507	35.48196189506652	29.27621084112878	18.487687620402188
20	20.00935209922967	34.65805169470267	24.71608828966179	20.616507916405872
21	13.513783386872477	35.631010976539386	24.94161026587726	25.91359537071088
22	12.93000781777045	34.78469470510445	27.03170702600809	25.253590451117013
23	18.957484480142618	31.905027483968702	19.688360776711324	29.449127259177356
24	12.474580068825606	37.32242579815539	26.719970385019103	23.483023747999894
25	14.00671695043631	29.172948078801177	29.961787907053772	26.858547063708745
26	18.645747839153636	36.75642895935977	21.837395245529137	22.760427955957457
27	11.750766555529307	33.47881043246153	27.501260341497748	27.26916267051142
28	15.67840468773974	28.146165517543707	35.187273351631625	20.988156443084925
29	14.888347138233282	31.24015167935938	27.492005659968388	26.37949552243895
30	16.86032493673938	28.67100337795876	30.263295689510304	24.205375995791556
31	20.581194000043837	29.92720462344406	24.148386641110754	25.343214735401347
32	20.448949471874293	30.096467877731044	21.8902443479468	27.56433830244786
33	20.210032561866328	24.42237392322998	28.234084992072635	27.133508522831058
34	13.024990075571782	27.182217372985583	25.345163089407528	34.44762946203511
35	14.036672893281343	24.38900836087413	29.503924715601197	32.07039403024333
36	18.387834477585404	21.182991843703043	32.79956551705662	27.629608161654932
37	14.635791750182051	29.053124307421037	26.335901101550647	29.975182840846266
38	18.924118917786767	26.59503218437274	28.545821633061617	25.935027264778874
39	15.973093231174637	26.362203880634095	28.411628750885892	29.25307413730538
40	18.925580183291405	25.03001682890773	20.687866381882255	35.35653660591861
41	15.57343711565673	18.68690681753421	31.484670107135116	34.254985959673945
42	23.365391874876707	21.441148749522043	24.632796155897545	30.560663219703706
43	18.8793067756446	21.33764244294367	30.009766124455982	29.773284656955745
44	19.559769412303368	22.493016368609094	27.282314060053142	30.664900159034396
45	23.75725457436989	26.679542039390846	25.61013923424817	23.953064151991097
46	17.402697983210057	23.247516457502744	34.82853267024352	24.521252889043673
47	20.23779660645441	23.4126394595266	24.738737904983648	31.610826029035344
48	20.088503980730778	17.670596659059967	37.19359088949667	25.047308470712586
49	21.109197935718928	16.314542270757883	32.56162278405175	30.014637009471436
50	18.25534640516509	20.546854260684896	25.926990304503377	35.27080902964664
51	16.015957019310623	21.171301719665955	35.30758421151331	27.50515704951011
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5136.0
1	4227.5
2	3319.0
3	2509.5
4	1700.0
5	1625.0
6	1550.0
7	1728.5
8	1907.0
9	2086.0
10	2265.0
11	2426.5
12	2588.0
13	2674.0
14	2760.0
15	2742.0
16	2724.0
17	2650.5
18	2577.0
19	2446.0
20	2315.0
21	2103.0
22	1891.0
23	1867.5
24	1844.0
25	1939.5
26	2294.5
27	2554.0
28	2604.0
29	2654.0
30	3070.5
31	3487.0
32	3554.0
33	3621.0
34	4493.5
35	5366.0
36	5395.5
37	5425.0
38	6966.5
39	8508.0
40	11267.0
41	14026.0
42	21813.0
43	29600.0
44	43937.5
45	58275.0
46	57463.5
47	56652.0
48	54645.5
49	52639.0
50	46459.0
51	40279.0
52	34645.0
53	29011.0
54	23625.5
55	18240.0
56	15373.5
57	12507.0
58	10285.0
59	8063.0
60	7974.0
61	7885.0
62	6838.5
63	5792.0
64	4751.0
65	3710.0
66	2885.0
67	2060.0
68	1770.0
69	1480.0
70	1459.5
71	1439.0
72	1356.0
73	1273.0
74	976.5
75	545.0
76	410.0
77	287.0
78	164.0
79	137.5
80	111.0
81	66.5
82	22.0
83	19.0
84	16.0
85	20.5
86	25.0
87	17.0
88	9.0
89	6.0
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48608842703274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94019474141034	29.167050142093153
2	9.20339437238114	6.715917217991076
3	3.4693823664102736	3.7975257542410006
4	1.7417984718915918	2.5420565227002845
5	1.0143370140930974	1.8504594995506551
6	0.6860500009641604	1.501876860032655
7	0.44517632290857295	1.1369919878284431
8	0.3208639949296962	0.9365657673644704
9	0.2611569809564926	0.8575737030463946
>10	2.168491435864142	17.463147229790817
>50	0.6172656505197998	15.832056103202138
>100	0.11649048068378	6.439421467470218
>500	0.007364340732882643	1.7565389489635967
>1k	0.005355884169369195	4.022378928185725
>5k	0.0026779420846845974	5.980439867539388
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	6579	1.6022776258332256	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	6457	1.5725652272389632	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	6016	1.465162212648227	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	5431	1.3226888259462304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4321	1.0523547075885953	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3272	0.7968767885280916	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2712	0.6604920080954109	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	1514	0.3687259956697832	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1359	0.33097663680002337	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1197	0.291522468174855	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1073	0.26132298107904717	No Hit
AACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1019	0.24817159153732435	TruSeq Adapter, Index 19 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	945	0.2301493169801487	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.19556603337043324	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	739	0.17997920132098402	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTAT	717	0.17462122780398584	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	688	0.16755844453157914	TruSeq Adapter, Index 19 (95% over 22bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	600	0.14612655046358647	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	565	0.13760250168654392	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	554	0.13492351492804486	No Hit
TAAAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	547	0.13321870517263634	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	528	0.1285913644079561	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	505	0.12298984664018528	No Hit
CAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	437	0.10642883758764547	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	436	0.10618529333687285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.10738840193569	0.0
2	0.0	0.0	0.0	2.6395325898739173	0.0
3	0.0	0.0	0.0	3.5481961895066525	0.0
4	0.0	0.0	0.0	9.06520410225936	0.0
5	0.0	0.0	0.0	9.901291515161848	0.0
6	0.0	0.0	0.0	13.704965623729004	0.0
7	0.0	0.0	0.0	16.788966471262995	0.0
8	0.0	0.0	0.0	18.535178749302855	0.0
9	0.0	0.0	0.0	22.238999715053225	0.0
10	0.0	0.0	0.0	25.4825220468433	0.0
11	0.0	0.0	0.0	30.36046984556859	0.0
12	0.0	0.0	0.0	32.17804058908484	0.0
13	0.0	0.0	0.0	32.977839908622194	0.0
14	0.0	0.0	0.0	33.77008935638561	0.0
15	0.0	0.0	0.0	34.271790512977255	0.0
16	0.0	0.0	0.0	35.14514019624796	0.0
17	0.0	0.0	0.0	36.169730859248475	0.0
18	0.0	0.0	0.0	37.65608142171392	0.0
19	0.0	0.0	0.0	38.210875224974004	0.0
20	0.0	0.0	0.0	38.74423713416609	0.0
21	0.0	0.0	0.0	39.272241069841186	0.0
22	0.0	0.0	0.0	39.7929386779931	0.0
23	0.0	0.0	0.0	40.320699069417415	0.0
24	0.0	0.0	0.0	40.66921089227307	0.0
25	0.0	0.0	0.0	40.95781082943866	0.0
26	0.0	0.0	0.0	41.246654310855014	0.0
27	0.0	0.0	0.0	41.58566790793053	0.0
28	0.0	0.0	0.0	41.930770111275365	0.0
29	0.0	0.0	0.0	42.225458654710266	0.0
30	0.0	0.0	0.0	42.774650940202584	0.0
31	0.0	0.0	0.0	43.11804833379201	0.0
32	0.0	0.0	0.0	43.38594700964192	0.0
33	0.0	0.0	0.0	43.666022898030455	0.0
34	0.0	0.0	0.0	43.95145675993599	0.0
35	0.0	0.0	0.0	44.24760656887553	0.0
36	0.0	0.0	0.0	44.54643536457357	0.0
37	0.0	0.0	0.0	44.80361809338948	0.0
38	0.0	0.0	0.0	45.064210441716206	0.0
39	0.0	0.0	0.0	45.31579165276435	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGC	30	2.16208E-6	45.000004	39
GCAAGAC	65	0.0	45.000004	45
GTCCTCG	30	2.16208E-6	45.000004	22
TATGGGT	35	1.2093733E-7	45.000004	25
GGGTATA	30	2.16208E-6	45.000004	7
GATCAGT	30	2.16208E-6	45.000004	40
ACTTATA	35	1.2093733E-7	45.000004	9
CGACAGG	30	2.16208E-6	45.000004	2
TCGATCA	30	2.16208E-6	45.000004	17
TAGCCGT	35	1.2093733E-7	45.000004	44
GCCGCGA	30	2.16208E-6	45.000004	26
TTATAGG	35	1.2093733E-7	45.000004	2
TATTGCG	30	2.16208E-6	45.000004	1
TGGTTAT	30	2.16208E-6	45.000004	17
AATGCGG	60	0.0	45.000004	2
GTCACTT	35	1.2093733E-7	45.000004	6
TGCGAAG	30	2.16208E-6	45.000004	1
GTCACGG	35	1.2093733E-7	45.000004	2
ACTACGG	60	0.0	45.000004	2
GATATTG	30	2.16208E-6	45.000004	23
>>END_MODULE