##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474853
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303434957765877	31.0	31.0	34.0	30.0	34.0
2	31.478238528555153	31.0	31.0	34.0	30.0	34.0
3	31.621714509542954	31.0	31.0	34.0	30.0	34.0
4	35.43177362257372	37.0	35.0	37.0	33.0	37.0
5	34.98442465352436	35.0	35.0	37.0	32.0	37.0
6	34.989691546647066	36.0	35.0	37.0	32.0	37.0
7	34.89548344435015	36.0	35.0	37.0	32.0	37.0
8	35.27394793757226	37.0	35.0	37.0	33.0	37.0
9	36.2238903408002	39.0	37.0	39.0	32.0	39.0
10	35.997411830608634	38.0	35.0	39.0	31.0	39.0
11	36.21776212849029	38.0	35.0	39.0	32.0	39.0
12	35.60726161570002	37.0	35.0	39.0	30.0	39.0
13	35.42078495871354	37.0	35.0	39.0	30.0	39.0
14	36.28469863305065	38.0	35.0	40.0	31.0	41.0
15	36.44392264553451	38.0	35.0	40.0	31.0	41.0
16	36.39007440197282	38.0	35.0	40.0	31.0	41.0
17	36.34777920745999	38.0	35.0	40.0	31.0	41.0
18	36.05251309352579	38.0	35.0	40.0	30.0	41.0
19	36.07329004976277	38.0	34.0	40.0	30.0	41.0
20	36.18020313654963	38.0	34.0	40.0	30.0	41.0
21	36.34351894165142	38.0	35.0	40.0	31.0	41.0
22	36.268365157217076	38.0	34.0	40.0	31.0	41.0
23	36.239979530507334	38.0	35.0	40.0	31.0	41.0
24	35.873065980419206	37.0	34.0	40.0	30.0	41.0
25	35.90062398257987	37.0	34.0	40.0	30.0	41.0
26	35.834395065420246	37.0	34.0	40.0	30.0	41.0
27	35.73402505617528	37.0	34.0	40.0	30.0	41.0
28	35.7571564252516	38.0	34.0	40.0	30.0	41.0
29	35.51452344199152	37.0	34.0	40.0	29.0	41.0
30	35.50981672222772	37.0	34.0	40.0	29.0	41.0
31	35.40751980086469	37.0	34.0	40.0	29.0	41.0
32	35.265092565488686	37.0	34.0	40.0	28.0	41.0
33	35.32234186158664	37.0	34.0	40.0	29.0	41.0
34	35.240626046376455	37.0	34.0	40.0	28.0	41.0
35	35.12143758173582	37.0	34.0	40.0	27.0	41.0
36	35.00350003053577	37.0	34.0	40.0	27.0	41.0
37	34.74907602984503	37.0	33.0	40.0	26.0	41.0
38	34.81821110954338	37.0	34.0	40.0	27.0	41.0
39	34.60260333197853	37.0	33.0	40.0	26.0	41.0
40	34.44134500571756	36.0	33.0	40.0	25.0	41.0
41	34.471152124973415	36.0	33.0	40.0	25.0	41.0
42	34.44394791651311	36.0	33.0	40.0	25.0	41.0
43	34.34111609276976	36.0	33.0	40.0	25.0	41.0
44	34.19849932505428	36.0	33.0	39.0	24.0	41.0
45	33.79817964717502	35.0	33.0	39.0	23.0	40.0
46	33.647137114012125	35.0	33.0	39.0	23.0	40.0
47	33.50414128161768	35.0	32.0	39.0	23.0	40.0
48	33.45313602314822	35.0	33.0	39.0	22.0	40.0
49	33.37103693142931	35.0	32.0	39.0	22.0	40.0
50	33.00765078877042	35.0	32.0	39.0	20.0	40.0
51	30.870254584050222	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	6.0
12	6.0
13	12.0
14	21.0
15	52.0
16	82.0
17	191.0
18	370.0
19	729.0
20	1157.0
21	1760.0
22	2577.0
23	3403.0
24	4048.0
25	4682.0
26	5554.0
27	6998.0
28	8862.0
29	11421.0
30	14446.0
31	18944.0
32	25064.0
33	33629.0
34	53983.0
35	56627.0
36	48292.0
37	59727.0
38	67607.0
39	44590.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.03042204640173	42.041642360899054	16.951561851773075	22.97637374092614
2	19.557842111137553	41.51432127416274	17.924073344803553	21.003763269896158
3	18.22774627095122	28.66150155942997	30.533449299046232	22.577302870572577
4	26.771021768842147	28.738578044152614	18.319774751344102	26.170625435661144
5	15.155848230926203	40.789465371388616	18.835302714734876	25.2193836829503
6	20.737365037179927	33.16373698807842	26.523576770074108	19.57532120466755
7	68.30324331951151	14.787523717866371	9.950026639823271	6.959206322798845
8	71.49033490364386	6.071773791046913	6.547710554634803	15.890180750674418
9	69.3507253823815	9.944340669638814	12.652757800835207	8.052176147144486
10	44.190517907647205	24.15105306273731	14.45057733656521	17.20785169305027
11	35.43201790870016	21.691133887750524	23.697649588398935	19.179198615150373
12	31.9804234152464	22.039031026443972	31.11383944083748	14.866706117472145
13	25.375853158767033	33.8544770697458	20.57226131034236	20.197408461144818
14	15.445411527356887	32.378651919646714	35.638397567247125	16.537538985749272
15	14.505962897991589	28.315078561154717	32.71012292225173	24.468835618601968
16	15.590930245781326	24.220548253880676	37.84076335202684	22.34775814831116
17	15.444779752891948	26.34878583477413	28.94727420907102	29.25916020326291
18	18.46803115911661	27.191362379515343	28.02698940514222	26.31361705622582
19	20.606798314425728	31.071721143174834	29.87261320871933	18.44886733368011
20	23.879811225789876	31.406561609592863	24.517482252402324	20.19614491221494
21	18.886055263418363	28.975914651481617	25.937290066610085	26.20074001848993
22	18.33683266189747	32.47510282129417	25.96740464943888	23.22065986736948
23	22.99974939612891	26.771232360330462	20.56468001676308	29.66433822677755
24	17.61008143572853	34.46161233055282	25.537587421791585	22.39071881192706
25	17.853314604730304	24.555809903275332	28.645917789294796	28.94495770269957
26	23.95899362539565	31.722659433551016	20.649337795064998	23.669009145988337
27	15.594720892570965	30.431101835726004	28.745738154755262	25.228439116947772
28	20.87993547476798	25.610662668236273	29.794904949531748	23.714496907463996
29	22.073568030527344	29.965062872088836	27.550420867089393	20.41094823029443
30	21.946370771586153	29.754681975263924	23.047764255464323	25.2511829976856
31	27.147559349946192	28.274013220933636	20.438114532286832	24.140312896833336
32	25.5203189197499	29.260423752192782	19.36641444826083	25.852842879796484
33	26.157147580409095	24.654998494270856	23.887603110857466	25.300250814462583
34	21.779792904330392	25.09639825377538	26.486302076642666	26.637506765251562
35	24.498739609942447	27.123551920278487	26.798398662322864	21.5793098074562
36	26.572433995362776	23.665429090687013	27.766277142610452	21.995859771339763
37	21.63195767953451	34.39506542024585	20.596689922986695	23.376286977232954
38	24.49263245678136	25.87411262011612	22.35807713123851	27.275177791864007
39	20.45727835772334	27.55336914792578	29.129646437950267	22.85970605640061
40	24.873381867651673	25.41565495005823	21.83454669129183	27.876416490998267
41	18.753593217269344	19.79538930995487	35.733163736988075	25.717853735787706
42	30.450055069674193	22.00575757129048	22.04155812430373	25.50262923473159
43	22.820746631062665	21.45906206762935	27.630235041160105	28.089956260147876
44	19.770960697310535	23.62731203130232	25.989937938688396	30.611789332698752
45	27.382579450903755	27.525360479980122	22.087467068756013	23.00459300036011
46	18.693574643100074	23.703546150071706	35.75927708153892	21.843602125289298
47	23.320480232829947	25.87621853499925	23.27014886712309	27.53315236504771
48	21.27437333237865	18.416225652991557	35.894055634059384	24.415345380570407
49	24.083453194988767	18.54763474169901	30.695394153559104	26.673517909753127
50	20.33155523920034	21.897302954809174	25.34321147807848	32.42793032791201
51	17.91880855759572	22.5872006705233	32.860695836395685	26.633294935485296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3317.0
1	2617.5
2	1918.0
3	1350.5
4	783.0
5	762.0
6	741.0
7	777.0
8	813.0
9	898.5
10	984.0
11	1022.0
12	1060.0
13	1127.5
14	1195.0
15	1207.5
16	1220.0
17	1342.5
18	1465.0
19	1510.0
20	1555.0
21	1448.0
22	1341.0
23	1551.0
24	1761.0
25	1838.5
26	2152.0
27	2388.0
28	3149.0
29	3910.0
30	4188.5
31	4467.0
32	4626.0
33	4785.0
34	5465.0
35	6145.0
36	7118.0
37	8091.0
38	9481.0
39	10871.0
40	13692.0
41	16513.0
42	22409.0
43	28305.0
44	48936.5
45	69568.0
46	66827.5
47	64087.0
48	55143.0
49	46199.0
50	42262.5
51	38326.0
52	33877.0
53	29428.0
54	26622.0
55	23816.0
56	21889.5
57	19963.0
58	18430.0
59	16897.0
60	15990.0
61	15083.0
62	14335.5
63	13588.0
64	12216.0
65	10844.0
66	9129.0
67	7414.0
68	6004.5
69	4595.0
70	4011.0
71	3427.0
72	2739.5
73	2052.0
74	1866.0
75	1303.5
76	927.0
77	795.0
78	663.0
79	535.5
80	408.0
81	283.5
82	159.0
83	123.5
84	88.0
85	65.0
86	42.0
87	33.0
88	24.0
89	22.5
90	21.0
91	13.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.14776550218595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33447237874418	22.330881247565397
2	9.139433096468668	5.145092392446346
3	3.167987621668185	2.6751531806563156
4	1.4442754847630224	1.6261251066266196
5	0.8061052911713378	1.1345031352981072
6	0.5593251644921116	0.9446252141757325
7	0.4058004543151472	0.7995663220120303
8	0.32951460025352786	0.7420079757986277
9	0.23460768500136106	0.5943313892186117
>10	3.287419660685625	22.729378421745235
>50	1.1431247688242694	22.530215259058515
>100	0.13523240729228286	5.837809854909881
>500	0.005229982602463977	0.9316437068068291
>1k	0.004482842230683409	2.6868352140325165
>5k	7.471403717805682E-4	1.1114530452537452
>10k+	0.0022414211153417045	8.180378534395494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	14252	3.001349891440088	No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	12653	2.6646141016272407	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	11993	2.5256237193405116	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	5285	1.112976015735396	TruSeq Adapter, Index 13 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	2928	0.6166118777811238	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2810	0.5917620821601632	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	2418	0.5092102187413788	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	1828	0.384961240636576	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	1439	0.30304115168273127	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	1353	0.2849302836877939	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	985	0.20743261598852697	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	747	0.15731184176997934	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	643	0.1354103269854039	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	534	0.11245585475926234	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	514	0.10824402499299782	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGG	506	0.10655929308649202	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	501	0.1055063356449259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5638523922140115	0.0
2	0.0	0.0	0.0	1.8748960204526453	0.0
3	0.0	0.0	0.0	2.768646296853974	0.0
4	0.0	0.0	0.0	7.763665808155366	0.0
5	0.0	0.0	0.0	8.26339940992265	0.0
6	0.0	0.0	0.0	10.407852535416223	0.0
7	0.0	0.0	0.0	12.167976194738161	0.0
8	0.0	0.0	0.0	12.957062501447817	0.0
9	0.0	0.0	0.0	16.934714532708018	0.0
10	0.0	0.0	0.0	18.569325664995272	0.0
11	0.0	0.0	0.0	23.598460997403407	0.0
12	0.0	0.0	0.0	24.543174413976537	0.0
13	0.0	0.0	0.0	25.080393300663573	0.0
14	0.0	0.0	0.0	25.835995560731426	0.0
15	0.0	0.0	0.0	26.1874727547262	0.0
16	0.0	0.0	0.0	26.776707739026605	0.0
17	0.0	0.0	0.0	27.408903386942907	0.0
18	0.0	0.0	0.0	28.412582420243737	0.0
19	0.0	0.0	0.0	28.767007895074897	0.0
20	0.0	0.0	0.0	29.16734231435834	0.0
21	0.0	0.0	0.0	29.570414422989852	0.0
22	0.0	0.0	0.0	29.965062872088836	0.0
23	0.0	0.0	0.0	30.36813498072035	0.0
24	0.0	0.0	0.0	30.650538166548383	0.0
25	2.1059148831322535E-4	0.0	0.0	30.90493268443076	0.0
26	2.1059148831322535E-4	0.0	0.0	31.135740955622055	0.0
27	2.1059148831322535E-4	0.0	0.0	31.396242626665515	0.0
28	2.1059148831322535E-4	0.0	0.0	31.62768267232175	0.0
29	2.1059148831322535E-4	0.0	0.0	31.852594381840273	0.0
30	2.1059148831322535E-4	0.0	0.0	32.227657822526126	0.0
31	2.1059148831322535E-4	0.0	0.0	32.49405605524236	0.0
32	2.1059148831322535E-4	0.0	0.0	32.73012911364148	0.0
33	2.1059148831322535E-4	0.0	0.0	32.9596738359029	0.0
34	2.1059148831322535E-4	0.0	0.0	33.18332199649154	0.0
35	2.1059148831322535E-4	0.0	0.0	33.44929904623115	0.0
36	2.1059148831322535E-4	0.0	0.0	33.6826344152822	0.0
37	2.1059148831322535E-4	0.0	0.0	33.937239524652895	0.0
38	2.1059148831322535E-4	0.0	0.0	34.178787961748164	0.0
39	2.1059148831322535E-4	0.0	0.0	34.4453967859527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.8873353E-5	45.000004	1
GGTGTAT	25	3.8873353E-5	45.000004	36
AATCAGT	25	3.8873353E-5	45.000004	38
CCGATTT	25	3.8873353E-5	45.000004	45
CACGGCA	25	3.8873353E-5	45.000004	40
CGACGAT	25	3.8873353E-5	45.000004	20
TCCGATT	25	3.8873353E-5	45.000004	44
CGGCCAA	25	3.8873353E-5	45.000004	26
ATTACGG	50	2.1827873E-11	45.000004	2
CAACCGA	35	1.209919E-7	45.000004	24
CGACAAT	50	2.1827873E-11	45.000004	20
AGCGGTG	25	3.8873353E-5	45.000004	17
CTAACCG	25	3.8873353E-5	45.000004	1
GAGACGA	25	3.8873353E-5	45.000004	16
ATCCCTC	25	3.8873353E-5	45.000004	42
CGCTACG	50	2.1827873E-11	45.000004	1
ACTACTA	25	3.8873353E-5	45.000004	27
CGGTGAT	25	3.8873353E-5	45.000004	41
CATACGA	70	0.0	45.000004	18
ATGACGG	25	3.8873353E-5	45.000004	2
>>END_MODULE