FastQCFastQC Report
Sat 18 Jun 2016
SRR3548851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479213
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC131532.744708511663916No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT117412.4500587421459765No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT113682.372222790283235No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT51531.0753047183611464TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27540.5746922558444784No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT26950.5623804028688704No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT21070.4396792240611169No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG18860.3935619442711279TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT14480.30216208658780125No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT12490.26063566722939485No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT9280.19365084002312125No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC7670.160054088682903No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7310.15254177161304056No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5620.11727561647951956No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5330.11122402772879701No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT4970.10371171065893453No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG4880.10183363139146892No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC4830.1007902540206547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAT207.029164E-445.00000425
GTTGAGC207.029164E-445.00000437
CTGCTAG207.029164E-445.00000420
GTTTGCG207.029164E-445.00000424
GTACCGC207.029164E-445.00000422
TCGCAAA207.029164E-445.00000438
GATCGGA207.029164E-445.00000422
AGTCAAT207.029164E-445.00000413
CGAGTCG207.029164E-445.00000416
ACGTTTA207.029164E-445.00000428
CAACGCG406.8012014E-945.0000041
CACGGAA406.8012014E-945.00000430
GCGGTCA207.029164E-445.00000419
GGCACGA406.8012014E-945.0000048
GACGTGT207.029164E-445.00000413
GACCGTC207.029164E-445.00000441
GAGTACA207.029164E-445.0000049
GACCGGT207.029164E-445.00000410
TAGCGCG207.029164E-445.0000041
CGACCTT207.029164E-445.00000422
Produced by FastQC (version 0.11.5)