##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479213
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.105007585353487	31.0	31.0	34.0	28.0	34.0
2	31.309263312973563	31.0	31.0	34.0	28.0	34.0
3	31.243063105550142	31.0	31.0	34.0	28.0	34.0
4	35.236118385770006	37.0	35.0	37.0	33.0	37.0
5	34.853027776792366	35.0	35.0	37.0	32.0	37.0
6	34.91662788780772	35.0	35.0	37.0	32.0	37.0
7	34.74507995400793	35.0	35.0	37.0	32.0	37.0
8	35.17516845327652	37.0	35.0	37.0	32.0	37.0
9	35.72874275113572	39.0	35.0	39.0	32.0	39.0
10	35.669163816507485	37.0	35.0	39.0	30.0	39.0
11	36.035683506081845	37.0	35.0	39.0	31.0	39.0
12	35.47889143241106	37.0	35.0	39.0	30.0	39.0
13	35.27476091007548	37.0	35.0	39.0	30.0	39.0
14	36.13351056836939	38.0	35.0	40.0	30.0	41.0
15	36.33717365764284	38.0	35.0	40.0	31.0	41.0
16	36.27893233280399	38.0	35.0	40.0	31.0	41.0
17	36.25400604741524	38.0	35.0	40.0	31.0	41.0
18	36.01170878085528	38.0	34.0	40.0	30.0	41.0
19	36.00771890578928	38.0	34.0	40.0	30.0	41.0
20	36.11193143758621	38.0	34.0	40.0	30.0	41.0
21	36.227381143666804	38.0	34.0	40.0	31.0	41.0
22	36.17898512769896	38.0	34.0	40.0	30.0	41.0
23	36.158367990851666	38.0	34.0	40.0	31.0	41.0
24	35.768249191904225	37.0	34.0	40.0	30.0	41.0
25	35.809214274237135	37.0	34.0	40.0	30.0	41.0
26	35.75458094834656	37.0	34.0	40.0	30.0	41.0
27	35.63194654569054	37.0	34.0	40.0	29.0	41.0
28	35.60543641345289	37.0	34.0	40.0	29.0	41.0
29	35.37304497165144	37.0	34.0	40.0	29.0	41.0
30	35.42300605367551	37.0	34.0	40.0	29.0	41.0
31	35.39123521273421	37.0	34.0	40.0	29.0	41.0
32	35.23332004766148	37.0	34.0	40.0	28.0	41.0
33	35.23912957286217	37.0	34.0	40.0	28.0	41.0
34	35.2275564310651	37.0	34.0	40.0	28.0	41.0
35	35.055059023857865	37.0	34.0	40.0	27.0	41.0
36	34.98911340051293	37.0	34.0	40.0	27.0	41.0
37	34.71652897563296	37.0	33.0	40.0	26.0	41.0
38	34.802348851137175	37.0	33.0	40.0	27.0	41.0
39	34.54135008858274	36.0	33.0	40.0	25.0	41.0
40	34.35766976271512	36.0	33.0	40.0	25.0	41.0
41	34.39189671398731	36.0	33.0	40.0	25.0	41.0
42	34.41469659629434	36.0	33.0	40.0	25.0	41.0
43	34.347213034704815	36.0	33.0	40.0	25.0	41.0
44	34.31908775429715	36.0	33.0	40.0	25.0	41.0
45	33.9853927168086	36.0	33.0	39.0	23.0	40.0
46	33.716781472956704	35.0	33.0	39.0	23.0	40.0
47	33.57350697915123	35.0	32.0	39.0	23.0	40.0
48	33.462026280589214	35.0	32.0	39.0	23.0	40.0
49	33.39359324559225	35.0	32.0	39.0	22.0	40.0
50	33.177411714623766	35.0	32.0	39.0	22.0	40.0
51	31.163720516763945	34.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	10.0
11	3.0
12	10.0
13	9.0
14	23.0
15	32.0
16	103.0
17	179.0
18	435.0
19	816.0
20	1316.0
21	1846.0
22	2695.0
23	3471.0
24	4012.0
25	4800.0
26	5841.0
27	7054.0
28	8947.0
29	11679.0
30	14944.0
31	19736.0
32	26057.0
33	35269.0
34	54804.0
35	56972.0
36	49631.0
37	60429.0
38	66668.0
39	41414.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.348208416716574	41.29938044251721	17.11681444368162	23.235596697084596
2	19.779513493999538	40.70549004304975	18.219873000106425	21.29512346284429
3	18.62219931429239	28.809735962922538	29.801987842566874	22.766076880218193
4	26.532043162435077	28.88047694866375	18.433765361123342	26.153714527777836
5	15.473912435597532	40.62681938929036	18.44816396884058	25.451104206271534
6	20.485671298566608	33.55188611327322	26.170199890236702	19.79224269792347
7	69.30988933939605	14.558661805919288	9.349704619866323	6.781744234818338
8	72.52328296602973	6.163230129399661	6.278836342085878	15.034650562484742
9	69.47933382441627	11.03288099446384	11.735073965021817	7.752711216098061
10	44.75233351348983	24.399797167439115	14.102705894873468	16.74516342419759
11	35.94956731140432	22.001072591937195	23.068656317754318	18.98070377890416
12	32.72386183179505	22.2813237537379	30.14943250704801	14.84538190741904
13	25.295849653494372	34.29310139749965	20.48713202688575	19.923916922120227
14	15.697195192951776	32.92022545298229	34.676855594485126	16.705723759580813
15	14.734157879690244	28.21709761630006	33.02059835605461	24.028146147955084
16	15.800698228136548	24.33802922708691	37.767130691362716	22.094141853413827
17	15.723696978170459	26.034143481082523	28.87713814107714	29.365021399669878
18	18.74510916857431	27.269502288126574	28.069355380592764	25.916033162706352
19	21.10084659639868	30.691362713448928	29.613345213923665	18.59444547622873
20	24.254141686473446	31.123112269491855	24.320083136308906	20.30266290772579
21	19.1096652219368	29.086439641662476	25.95150799331404	25.852387143086688
22	18.753247512066658	32.16961977241853	25.65456279358031	23.422569921934507
23	22.910480308338883	27.112160980607786	20.65094227410358	29.32641643694975
24	18.014536333530184	33.90726044577255	25.775803244068925	22.302399976628347
25	18.021213948703398	24.879333407065335	28.790537819299562	28.308914824931712
26	23.662755392695942	31.674641547704258	20.84876662360996	23.813836435989842
27	15.76125856351977	30.8712409721773	28.627353598504214	24.740146865798714
28	20.72481339195723	25.98531342012842	29.91884610809807	23.37102707981628
29	21.72624699246473	30.02339252065366	27.787017464050436	20.463343022831186
30	21.8606339978256	29.765469634588378	23.29611258459182	25.0777837829942
31	27.358398040119948	28.48962778555674	20.530536525511618	23.621437648811696
32	25.759943908032547	29.103551030543827	19.576263582164923	25.5602414792587
33	26.167695784546748	25.078201133942528	23.834495307931963	24.919607773578765
34	21.95495531214721	25.521219165590246	26.260347695075048	26.263477827187494
35	24.226179172935623	27.348172941885966	26.829405713117133	21.596242172061274
36	26.590680970674835	24.03065025364504	27.574585831352653	21.804082944327472
37	21.880666843345235	34.43228793876627	20.823308215762093	22.863737002126403
38	24.222423014400697	26.218821275716643	22.451602940654784	27.10715276922788
39	20.538466193529807	27.687479262874753	28.841037284046966	22.933017259548468
40	24.81756546671313	25.400396066049964	22.256700047786683	27.525338419450225
41	18.772236980215478	20.160137558872567	35.097336674923255	25.970288785988693
42	29.937626900772724	22.20369647734932	22.36187248676476	25.496804135113194
43	23.01961758132605	21.669487263492435	27.32709671899552	27.983798436185996
44	19.77367058072298	24.10410402055036	25.94733448383078	30.17489091489588
45	26.779949625740535	28.00299657980898	22.28487123679867	22.932182557651817
46	18.98425126196493	24.234943542850466	35.12968137341851	21.651123821766106
47	23.263976561570743	26.2140217398109	23.369983702445467	27.15201799617289
48	21.328511538710345	18.89034729859165	35.41327134280581	24.3678698198922
49	24.043170782094812	19.012631126451076	30.51732736799711	26.426870723457004
50	20.46814255873693	22.470801084277763	25.407073681223174	31.653982675762133
51	18.002850506977065	23.366227543910536	32.30692823441768	26.323993714694716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3261.0
1	2695.0
2	2129.0
3	1475.5
4	822.0
5	785.0
6	748.0
7	822.5
8	897.0
9	930.5
10	964.0
11	1034.5
12	1105.0
13	1151.0
14	1197.0
15	1222.0
16	1247.0
17	1374.5
18	1502.0
19	1501.0
20	1500.0
21	1463.5
22	1427.0
23	1631.5
24	1836.0
25	1959.0
26	2328.5
27	2575.0
28	3380.0
29	4185.0
30	4474.0
31	4763.0
32	4924.5
33	5086.0
34	5811.0
35	6536.0
36	7530.5
37	8525.0
38	9944.5
39	11364.0
40	14299.5
41	17235.0
42	23158.5
43	29082.0
44	48275.5
45	67469.0
46	65340.5
47	63212.0
48	54656.5
49	46101.0
50	42211.5
51	38322.0
52	33759.5
53	29197.0
54	26655.5
55	24114.0
56	22251.5
57	20389.0
58	18894.5
59	17400.0
60	16439.0
61	15478.0
62	14822.5
63	14167.0
64	12586.0
65	11005.0
66	9360.5
67	7716.0
68	6321.5
69	4927.0
70	4235.0
71	3543.0
72	2827.0
73	2111.0
74	1883.0
75	1291.0
76	927.0
77	793.5
78	660.0
79	536.5
80	413.0
81	289.0
82	165.0
83	119.0
84	73.0
85	55.5
86	38.0
87	29.0
88	20.0
89	23.0
90	26.0
91	16.0
92	6.0
93	7.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	479213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.456023162026707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40284553866822	22.594892117791716
2	9.074452470053625	5.164456593411128
3	3.221071760214252	2.749766778456206
4	1.482578589191515	1.687531626942345
5	0.8644645281489834	1.229961131787898
6	0.5398929040613262	0.9217922989789776
7	0.38861787702436607	0.7740963516848104
8	0.29066009397910125	0.661682429323693
9	0.22441714502906582	0.5747417529212703
>10	3.193237949471643	22.224260558607092
>50	1.1536847984587206	22.923757559693996
>100	0.15303982020370782	6.543244264314742
>500	0.0036788418318199003	0.7371932373816782
>1k	0.00441461019818388	2.5415474889452403
>5k	7.3576836636398E-4	1.0788857575199624
>10k+	0.00220730509909194	7.592190052239254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	13153	2.744708511663916	No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	11741	2.4500587421459765	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	11368	2.372222790283235	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	5153	1.0753047183611464	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2754	0.5746922558444784	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	2695	0.5623804028688704	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	2107	0.4396792240611169	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	1886	0.3935619442711279	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	1448	0.30216208658780125	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	1249	0.26063566722939485	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	928	0.19365084002312125	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	767	0.160054088682903	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	731	0.15254177161304056	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	562	0.11727561647951956	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	533	0.11122402772879701	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	497	0.10371171065893453	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG	488	0.10183363139146892	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	483	0.1007902540206547	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.448833817112641	0.0
2	0.0	0.0	0.0	1.7236594165851093	0.0
3	0.0	0.0	0.0	2.5562745584948656	0.0
4	0.0	0.0	0.0	7.052605000281712	0.0
5	0.0	0.0	0.0	7.523585545467256	0.0
6	0.0	0.0	0.0	9.447156066300371	0.0
7	0.0	0.0	0.0	11.001788348813575	0.0
8	0.0	0.0	0.0	11.700433836310784	0.0
9	0.0	0.0	0.0	15.447828001327176	0.0
10	0.0	0.0	0.0	17.011433329229384	0.0
11	0.0	0.0	0.0	21.703918716729305	0.0
12	0.0	0.0	0.0	22.640454244772158	0.0
13	0.0	0.0	0.0	23.152543858367782	0.0
14	0.0	0.0	0.0	23.881029938670277	0.0
15	0.0	0.0	0.0	24.233482814531325	0.0
16	0.0	0.0	0.0	24.80796639490164	0.0
17	0.0	0.0	0.0	25.39371845087675	0.0
18	0.0	0.0	0.0	26.37324112659715	0.0
19	0.0	0.0	0.0	26.724024598664894	0.0
20	0.0	0.0	0.0	27.109448199443673	0.0
21	0.0	0.0	0.0	27.49278504548082	0.0
22	0.0	0.0	0.0	27.871531031086384	0.0
23	0.0	0.0	0.0	28.25236377143358	0.0
24	0.0	0.0	0.0	28.529693476596	0.0
25	0.0	0.0	0.0	28.750472128260295	0.0
26	0.0	0.0	0.0	28.96874667423463	0.0
27	0.0	0.0	0.0	29.211018899737695	0.0
28	0.0	0.0	0.0	29.428041392867055	0.0
29	0.0	0.0	0.0	29.64381183315144	0.0
30	0.0	0.0	0.0	30.004611727979	0.0
31	0.0	0.0	0.0	30.246466602533737	0.0
32	0.0	0.0	0.0	30.480183133596125	0.0
33	0.0	0.0	0.0	30.714525691081	0.0
34	0.0	0.0	0.0	30.933217588003664	0.0
35	0.0	0.0	0.0	31.203869677992877	0.0
36	0.0	0.0	0.0	31.435499454313636	0.0
37	0.0	0.0	0.0	31.686953400679865	2.0867547416284616E-4
38	0.0	0.0	0.0	31.936529267778628	2.0867547416284616E-4
39	0.0	0.0	0.0	32.207390033242	2.0867547416284616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAT	20	7.029164E-4	45.000004	25
GTTGAGC	20	7.029164E-4	45.000004	37
CTGCTAG	20	7.029164E-4	45.000004	20
GTTTGCG	20	7.029164E-4	45.000004	24
GTACCGC	20	7.029164E-4	45.000004	22
TCGCAAA	20	7.029164E-4	45.000004	38
GATCGGA	20	7.029164E-4	45.000004	22
AGTCAAT	20	7.029164E-4	45.000004	13
CGAGTCG	20	7.029164E-4	45.000004	16
ACGTTTA	20	7.029164E-4	45.000004	28
CAACGCG	40	6.8012014E-9	45.000004	1
CACGGAA	40	6.8012014E-9	45.000004	30
GCGGTCA	20	7.029164E-4	45.000004	19
GGCACGA	40	6.8012014E-9	45.000004	8
GACGTGT	20	7.029164E-4	45.000004	13
GACCGTC	20	7.029164E-4	45.000004	41
GAGTACA	20	7.029164E-4	45.000004	9
GACCGGT	20	7.029164E-4	45.000004	10
TAGCGCG	20	7.029164E-4	45.000004	1
CGACCTT	20	7.029164E-4	45.000004	22
>>END_MODULE