FastQCFastQC Report
Sat 18 Jun 2016
SRR3548850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335556
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT29430.8770518184744126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21290.6344693583187306No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT15920.47443645770005605No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC15750.46937023924471627No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT13830.412151771984408No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT13040.38860875680959367No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT8230.24526457580850886No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5350.15943687491804648No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4820.14364219385139887No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT4670.1391720010966873No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.1278475127847513No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT3660.10907270321496264No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC3550.10579456186150747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG351.208482E-745.0000041
CGACACG351.208482E-745.0000041
CCGCATG351.208482E-745.0000041
ACGCGCA351.208482E-745.00000415
ATTTACG207.0261396E-445.025
AACGTAG253.8848826E-545.01
CGGGTCA253.8848826E-545.06
TCGTTCA207.0261396E-445.016
ACCGGCT207.0261396E-445.045
TCCGCTG253.8848826E-545.041
CTCGTTC207.0261396E-445.015
ATAGGCG253.8848826E-545.01
ACGTTCA207.0261396E-445.019
TCCGAGC207.0261396E-445.017
TAGACCG207.0261396E-445.035
CGCATCA207.0261396E-445.020
AGCTTAG207.0261396E-445.01
GACCGAT406.7902874E-945.09
TAAACGG453.8380676E-1045.02
ATAGACG253.8848826E-545.01