##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391371335932007	31.0	31.0	34.0	30.0	34.0
2	31.54654662709056	31.0	31.0	34.0	30.0	34.0
3	31.766730441416634	31.0	31.0	34.0	30.0	34.0
4	35.606906149793176	37.0	35.0	37.0	33.0	37.0
5	35.22767585738297	37.0	35.0	37.0	33.0	37.0
6	35.22965168258055	37.0	35.0	37.0	32.0	37.0
7	35.231380157112376	37.0	35.0	37.0	32.0	37.0
8	35.548421127919035	37.0	35.0	37.0	33.0	37.0
9	36.37903956418601	39.0	37.0	39.0	32.0	39.0
10	36.03167578585989	38.0	35.0	39.0	31.0	39.0
11	36.27416586203197	38.0	35.0	39.0	32.0	39.0
12	36.04058637008428	37.0	35.0	39.0	32.0	39.0
13	35.787364851172384	37.0	35.0	39.0	31.0	39.0
14	36.80791581733004	38.0	35.0	40.0	31.0	41.0
15	36.845036297965166	38.0	35.0	40.0	32.0	41.0
16	36.864672364672366	38.0	35.0	40.0	32.0	41.0
17	36.837988294055236	38.0	35.0	40.0	32.0	41.0
18	36.6660318992955	38.0	35.0	40.0	31.0	41.0
19	36.68765571171429	38.0	35.0	40.0	31.0	41.0
20	36.63060115152165	38.0	35.0	40.0	31.0	41.0
21	36.669968649048144	38.0	35.0	40.0	31.0	41.0
22	36.627850492913254	38.0	35.0	40.0	31.0	41.0
23	36.53594332987638	38.0	35.0	40.0	31.0	41.0
24	36.218690769946	38.0	35.0	40.0	30.0	41.0
25	36.334027703274565	38.0	35.0	40.0	31.0	41.0
26	36.253152975956326	38.0	35.0	40.0	30.0	41.0
27	36.17764844020074	38.0	35.0	40.0	30.0	41.0
28	36.15062463493426	38.0	35.0	40.0	30.0	41.0
29	35.929484199358676	38.0	35.0	40.0	30.0	41.0
30	35.84722073215797	38.0	35.0	40.0	29.0	41.0
31	35.70289012862234	38.0	34.0	40.0	29.0	41.0
32	35.676706719593746	38.0	34.0	40.0	29.0	41.0
33	35.585625052152245	38.0	34.0	40.0	29.0	41.0
34	35.56644196497753	38.0	34.0	40.0	29.0	41.0
35	35.45843614776669	38.0	34.0	40.0	28.0	41.0
36	35.33399492186103	38.0	34.0	40.0	27.0	41.0
37	35.20342953188141	38.0	34.0	40.0	27.0	41.0
38	35.13312234023531	38.0	34.0	40.0	27.0	41.0
39	35.11302435361013	38.0	34.0	40.0	27.0	41.0
40	34.95642456102707	37.0	34.0	40.0	26.0	41.0
41	35.049711523560894	38.0	34.0	40.0	26.0	41.0
42	34.974671887851805	37.0	34.0	40.0	26.0	41.0
43	34.928810690316965	37.0	34.0	40.0	26.0	41.0
44	34.77630559429723	37.0	34.0	40.0	26.0	41.0
45	34.51988639750146	37.0	33.0	40.0	24.0	41.0
46	34.48115664747464	37.0	33.0	40.0	24.0	41.0
47	34.36718163287201	37.0	33.0	40.0	24.0	41.0
48	34.391341534646976	37.0	33.0	40.0	24.0	41.0
49	34.26887017368189	37.0	33.0	40.0	24.0	41.0
50	34.007530784727436	36.0	33.0	40.0	23.0	41.0
51	32.166818653220325	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	6.0
12	7.0
13	4.0
14	14.0
15	26.0
16	62.0
17	109.0
18	279.0
19	527.0
20	875.0
21	1314.0
22	1871.0
23	2392.0
24	2936.0
25	3132.0
26	3550.0
27	4332.0
28	5330.0
29	6601.0
30	8562.0
31	11029.0
32	14206.0
33	19994.0
34	32170.0
35	36036.0
36	33233.0
37	45151.0
38	58584.0
39	43212.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.737653327611486	33.589326371753145	19.340735972535136	25.33228432810023
2	23.835067768122162	33.41439282861877	19.671530236383795	23.079009166875274
3	20.538151605097212	33.04813503558273	21.171726924865002	25.241986434455054
4	20.35874786920812	32.8973405333238	19.911728593736964	26.83218300373112
5	17.05378535922469	35.14286736044058	19.10798793643982	28.69535934389491
6	20.2970592091931	37.59283100287284	20.89308490982131	21.217024878112746
7	78.59254491053655	8.639392530605921	7.334096246230137	5.4339663126274
8	81.44184577238971	6.656117011765547	5.349926688838822	6.552110527005925
9	74.42692128884597	11.046740335443264	9.666642825638641	4.859695550072119
10	39.87650347482983	30.84403199465961	13.076207846082324	16.203256684428233
11	31.186448759670515	27.974466258985085	24.49248411591508	16.346600865429316
12	30.183337505513236	25.734899688874584	27.88983060949588	16.191932196116294
13	24.046060866144547	34.0426039170809	23.714968589445576	18.19636662732897
14	16.98941458355684	35.257900320661825	29.944331199561326	17.808353896220005
15	15.905541846964441	30.85893263717531	33.350916091501865	19.88460942435838
16	17.37742731466581	28.06476415263026	33.45462456341117	21.103183969292754
17	17.74785728760624	27.973870233284458	28.289465841767097	25.988806637342204
18	21.33742206963964	26.367282957241116	28.673008380121352	23.62228659299789
19	21.974275530760888	31.940719283815515	26.16731633468035	19.917688850743247
20	25.079271418183552	30.024496656295817	25.138873988246374	19.757357937274257
21	21.582686645448153	31.498766226799702	25.236324190299086	21.682222937453062
22	20.875502151652782	29.607576678706387	26.30499827152547	23.21192289811537
23	20.282158566677396	31.2833029360226	23.998080797243976	24.436457700056028
24	18.527160891177626	30.586846904838538	28.221816924745795	22.66417527923804
25	19.038848955166944	28.776418839180344	27.949135166708388	24.23559703894432
26	20.463946405369	31.75297118811763	24.797947287487037	22.98513511902633
27	17.18163287200944	30.748071856858466	27.434168961365614	24.636126309766475
28	19.50136489885444	28.791021468845734	31.119693881200156	20.58791975109967
29	18.854080987972203	29.108703167280574	27.64814218789114	24.389073656856084
30	20.582853532644325	28.824994933781547	29.011550978078176	21.580600555495952
31	25.636853461121245	28.656915686204385	22.544076100561455	23.16215475211291
32	22.709175219635473	26.917414678920952	25.964071570766134	24.409338530677445
33	22.873678313008856	28.744829477047052	26.08566081369429	22.295831396249806
34	19.43401399468345	25.83324392947824	26.793441333190287	27.939300742648022
35	18.871365733290418	27.091752196354708	30.951912646473318	23.084969423881557
36	22.943115307132047	26.16374018047658	28.462313294949276	22.430831217442098
37	19.82828499564901	29.0628091883322	26.77317645936893	24.33572935664986
38	23.035201277879104	28.821418779577773	26.06837606837607	22.075003874167052
39	20.172787850612117	26.133342869744542	27.456817937989484	26.23705134165385
40	22.37361275018179	26.794037358890915	24.898675630893205	25.933674260034095
41	19.000405297476426	22.355433966312628	31.161415680244133	27.482745055966813
42	23.543611200514967	24.927582877373673	27.59956609328994	23.92923982882142
43	20.965800045297954	25.087019752291717	28.229267246003648	25.71791295640668
44	21.520401959732503	25.329900225297713	26.435527899963045	26.714169915006735
45	23.110300516158258	27.123341558488	26.657547473447057	23.108810451906685
46	20.149840861137932	25.83503200658012	30.339794251928144	23.6753328803538
47	20.567356864427992	26.793441333190287	26.904898139207763	25.734303663173957
48	21.3407002109931	22.453778206916283	32.79661218991763	23.40890939217299
49	21.38808425419304	21.09752172513679	32.0712489122531	25.443145108417077
50	19.41076899235895	25.02056288667167	27.625493211267273	27.94317490970211
51	19.278153273969174	24.859337934651744	30.85058827736652	25.011920514012566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2494.0
1	2019.0
2	1544.0
3	1069.0
4	594.0
5	591.5
6	589.0
7	599.5
8	610.0
9	667.0
10	724.0
11	733.0
12	742.0
13	862.0
14	982.0
15	996.0
16	1010.0
17	1074.5
18	1139.0
19	1207.0
20	1275.0
21	1301.0
22	1327.0
23	1548.0
24	1769.0
25	1611.5
26	1916.0
27	2378.0
28	2614.0
29	2850.0
30	4087.5
31	5325.0
32	5815.5
33	6306.0
34	6979.0
35	7652.0
36	8737.5
37	9823.0
38	10289.5
39	10756.0
40	12594.0
41	14432.0
42	19428.5
43	24425.0
44	29018.5
45	33612.0
46	33796.5
47	33981.0
48	34883.0
49	35785.0
50	33453.5
51	31122.0
52	28805.0
53	26488.0
54	22542.5
55	18597.0
56	16140.5
57	13684.0
58	11940.0
59	10196.0
60	9505.0
61	8814.0
62	7778.0
63	6742.0
64	6133.5
65	5525.0
66	4541.5
67	3558.0
68	3039.0
69	2520.0
70	2012.5
71	1505.0
72	1383.0
73	1261.0
74	1020.0
75	634.0
76	489.0
77	386.0
78	283.0
79	231.0
80	179.0
81	159.0
82	139.0
83	89.0
84	39.0
85	32.5
86	26.0
87	16.5
88	7.0
89	7.0
90	7.0
91	6.0
92	5.0
93	7.0
94	9.0
95	6.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.767316900389634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94565172107953	23.674112506094648
2	9.827590219479317	6.047371652997797
3	3.207691606242627	2.9607619250396024
4	1.463202711166071	1.8007528601582317
5	0.9107264371031878	1.4010304449958273
6	0.6430878210460741	1.1871652070943373
7	0.49130196035317164	1.0581230175578085
8	0.37946075838370075	0.9339991523562803
9	0.3205444722631495	0.8876064022909629
>10	4.916013800999637	37.19159908025073
>50	0.8338150566102317	16.053417072947262
>100	0.05292478802436201	3.029966874766146
>500	0.0019971618122400754	0.4172272374703333
>1k	0.005991485436720227	3.356866565980016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	2943	0.8770518184744126	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2129	0.6344693583187306	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1592	0.47443645770005605	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	1575	0.46937023924471627	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	1383	0.412151771984408	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1304	0.38860875680959367	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	823	0.24526457580850886	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	535	0.15943687491804648	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	482	0.14364219385139887	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	467	0.1391720010966873	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	429	0.1278475127847513	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	366	0.10907270321496264	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	355	0.10579456186150747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1446673580564795	0.0
2	0.0	0.0	0.0	1.4733755319529378	0.0
3	0.0	0.0	0.0	1.9180107046215833	0.0
4	0.0	0.0	0.0	4.381980951018608	0.0
5	0.0	0.0	0.0	4.757775155264695	0.0
6	0.0	0.0	0.0	6.480587442930539	0.0
7	0.0	0.0	0.0	7.805850588277367	0.0
8	0.0	0.0	0.0	8.627174003743042	0.0
9	0.0	0.0	0.0	10.15210575880032	0.0
10	0.0	0.0	0.0	11.96610997866228	0.0
11	0.0	0.0	0.0	14.199716291766501	0.0
12	0.0	0.0	0.0	15.127728307644626	0.0
13	0.0	0.0	0.0	15.547032388036572	0.0
14	0.0	0.0	0.0	15.945177556056217	0.0
15	0.0	0.0	0.0	16.25838906173634	0.0
16	0.0	0.0	0.0	16.8764677132878	0.0
17	0.0	0.0	0.0	17.609579325060498	0.0
18	0.0	0.0	0.0	18.601068078055526	0.0
19	0.0	0.0	0.0	19.045405237873858	0.0
20	0.0	0.0	0.0	19.48765630773999	0.0
21	0.0	0.0	0.0	19.968351035296642	0.0
22	0.0	0.0	0.0	20.438317300241987	0.0
23	0.0	0.0	0.0	20.931826580362145	0.0
24	0.0	0.0	0.0	21.294806232044724	0.0
25	0.0	0.0	0.0	21.582686645448153	0.0
26	0.0	0.0	0.0	21.875633277306918	0.0
27	0.0	0.0	0.0	22.16321567786003	0.0
28	0.0	0.0	0.0	22.431725255993037	0.0
29	0.0	0.0	0.0	22.728546054905888	0.0
30	0.0	0.0	0.0	23.187783857239925	0.0
31	0.0	0.0	0.0	23.51559799258544	0.0
32	0.0	0.0	0.0	23.80318039313855	0.0
33	0.0	0.0	0.0	24.10774952615957	0.0
34	0.0	0.0	0.0	24.38370942555043	0.0
35	0.0	0.0	0.0	24.747583115783954	0.0
36	0.0	0.0	0.0	25.02831122077984	0.0
37	0.0	0.0	0.0	25.31797971128515	0.0
38	0.0	0.0	0.0	25.639833589624384	0.0
39	0.0	0.0	0.0	25.966753686418958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	35	1.208482E-7	45.000004	1
CGACACG	35	1.208482E-7	45.000004	1
CCGCATG	35	1.208482E-7	45.000004	1
ACGCGCA	35	1.208482E-7	45.000004	15
ATTTACG	20	7.0261396E-4	45.0	25
AACGTAG	25	3.8848826E-5	45.0	1
CGGGTCA	25	3.8848826E-5	45.0	6
TCGTTCA	20	7.0261396E-4	45.0	16
ACCGGCT	20	7.0261396E-4	45.0	45
TCCGCTG	25	3.8848826E-5	45.0	41
CTCGTTC	20	7.0261396E-4	45.0	15
ATAGGCG	25	3.8848826E-5	45.0	1
ACGTTCA	20	7.0261396E-4	45.0	19
TCCGAGC	20	7.0261396E-4	45.0	17
TAGACCG	20	7.0261396E-4	45.0	35
CGCATCA	20	7.0261396E-4	45.0	20
AGCTTAG	20	7.0261396E-4	45.0	1
GACCGAT	40	6.7902874E-9	45.0	9
TAAACGG	45	3.8380676E-10	45.0	2
ATAGACG	25	3.8848826E-5	45.0	1
>>END_MODULE