FastQCFastQC Report
Sat 18 Jun 2016
SRR3548848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366419
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT29570.806999637027556TruSeq Adapter, Index 15 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC22730.6203280943400861No Hit
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT19350.528083969444816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19110.5215340907540276No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT18330.5002469850089651No Hit
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT9020.24616627412879794No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT6090.16620317177875601No Hit
AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT4610.1258122531855608No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.12144566739170186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC207.0269883E-445.0000046
CGAACTT207.0269883E-445.00000421
TACCCGG351.2089004E-745.0000042
GCAAGCG207.0269883E-445.0000041
CCCTCGG207.0269883E-445.0000042
GTCGATA207.0269883E-445.00000437
CGTGCGC207.0269883E-445.00000417
CGCATAG302.1614396E-645.0000041
GGATCGA207.0269883E-445.0000048
TCGATAT207.0269883E-445.00000438
CACCTAG207.0269883E-445.0000041
ACGATTG207.0269883E-445.0000041
AGTGCGG406.7939254E-945.0000042
GTAGCGG406.7939254E-945.0000042
GCCGAGT207.0269883E-445.00000420
AAGCCCG207.0269883E-445.0000041
TGCGCGA207.0269883E-445.00000419
CCATCGA207.0269883E-445.00000410
CTCGGTA207.0269883E-445.00000422
CATAGCG302.1614396E-645.0000041