Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548848_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 366419 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT | 2957 | 0.806999637027556 | TruSeq Adapter, Index 15 (95% over 23bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC | 2273 | 0.6203280943400861 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT | 1935 | 0.528083969444816 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1911 | 0.5215340907540276 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT | 1833 | 0.5002469850089651 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT | 902 | 0.24616627412879794 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT | 609 | 0.16620317177875601 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT | 461 | 0.1258122531855608 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 445 | 0.12144566739170186 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGGTTC | 20 | 7.0269883E-4 | 45.000004 | 6 |
CGAACTT | 20 | 7.0269883E-4 | 45.000004 | 21 |
TACCCGG | 35 | 1.2089004E-7 | 45.000004 | 2 |
GCAAGCG | 20 | 7.0269883E-4 | 45.000004 | 1 |
CCCTCGG | 20 | 7.0269883E-4 | 45.000004 | 2 |
GTCGATA | 20 | 7.0269883E-4 | 45.000004 | 37 |
CGTGCGC | 20 | 7.0269883E-4 | 45.000004 | 17 |
CGCATAG | 30 | 2.1614396E-6 | 45.000004 | 1 |
GGATCGA | 20 | 7.0269883E-4 | 45.000004 | 8 |
TCGATAT | 20 | 7.0269883E-4 | 45.000004 | 38 |
CACCTAG | 20 | 7.0269883E-4 | 45.000004 | 1 |
ACGATTG | 20 | 7.0269883E-4 | 45.000004 | 1 |
AGTGCGG | 40 | 6.7939254E-9 | 45.000004 | 2 |
GTAGCGG | 40 | 6.7939254E-9 | 45.000004 | 2 |
GCCGAGT | 20 | 7.0269883E-4 | 45.000004 | 20 |
AAGCCCG | 20 | 7.0269883E-4 | 45.000004 | 1 |
TGCGCGA | 20 | 7.0269883E-4 | 45.000004 | 19 |
CCATCGA | 20 | 7.0269883E-4 | 45.000004 | 10 |
CTCGGTA | 20 | 7.0269883E-4 | 45.000004 | 22 |
CATAGCG | 30 | 2.1614396E-6 | 45.000004 | 1 |