##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290271519762893	31.0	31.0	34.0	30.0	34.0
2	31.461496265204588	31.0	31.0	34.0	30.0	34.0
3	31.665265720391137	31.0	31.0	34.0	30.0	34.0
4	35.51967010444327	37.0	35.0	37.0	33.0	37.0
5	35.115329718164176	37.0	35.0	37.0	32.0	37.0
6	35.12079340863875	37.0	35.0	37.0	32.0	37.0
7	35.19830303559586	37.0	35.0	37.0	32.0	37.0
8	35.50942773164056	37.0	35.0	37.0	33.0	37.0
9	36.36100748050729	39.0	37.0	39.0	32.0	39.0
10	35.85425428266547	38.0	35.0	39.0	30.0	39.0
11	36.18107685463909	38.0	35.0	39.0	31.0	39.0
12	36.040551936444345	37.0	35.0	39.0	32.0	39.0
13	35.85069551524348	37.0	35.0	39.0	31.0	39.0
14	36.89977048133421	39.0	35.0	40.0	31.0	41.0
15	36.93312846768317	38.0	35.0	40.0	32.0	41.0
16	36.90809155638763	38.0	35.0	40.0	32.0	41.0
17	36.87205630712381	38.0	35.0	40.0	32.0	41.0
18	36.703293224423405	38.0	35.0	40.0	31.0	41.0
19	36.77451769695349	38.0	35.0	40.0	31.0	41.0
20	36.73412404924417	38.0	35.0	40.0	31.0	41.0
21	36.77832754305863	38.0	35.0	40.0	31.0	41.0
22	36.79176571083923	38.0	35.0	40.0	31.0	41.0
23	36.7180713882195	38.0	35.0	40.0	31.0	41.0
24	36.353294998348886	38.0	35.0	40.0	30.0	41.0
25	36.48188276262967	38.0	35.0	40.0	31.0	41.0
26	36.342656903708594	38.0	35.0	40.0	30.0	41.0
27	36.25256605143292	38.0	35.0	40.0	30.0	41.0
28	36.23548451363057	38.0	35.0	40.0	30.0	41.0
29	36.03513464094384	38.0	35.0	40.0	30.0	41.0
30	35.917416946173645	38.0	35.0	40.0	30.0	41.0
31	35.710689129111756	38.0	34.0	40.0	29.0	41.0
32	35.60741118773863	38.0	34.0	40.0	29.0	41.0
33	35.677148291982675	38.0	34.0	40.0	29.0	41.0
34	35.384655271697156	38.0	34.0	40.0	27.0	41.0
35	35.43068181508055	38.0	34.0	40.0	28.0	41.0
36	35.24828406823882	38.0	34.0	40.0	27.0	41.0
37	34.9970853039826	38.0	34.0	40.0	25.0	41.0
38	34.97723371331727	38.0	34.0	40.0	26.0	41.0
39	34.75989782189242	37.0	33.0	40.0	25.0	41.0
40	34.63971846438094	37.0	33.0	40.0	24.0	41.0
41	34.64636386213597	37.0	33.0	40.0	24.0	41.0
42	34.57272139272254	37.0	33.0	40.0	24.0	41.0
43	34.53457380758094	37.0	33.0	40.0	24.0	41.0
44	34.34180269036267	37.0	33.0	40.0	23.0	41.0
45	33.98220889200615	37.0	33.0	40.0	23.0	41.0
46	33.89929015689689	37.0	33.0	40.0	22.0	41.0
47	33.75526378271869	37.0	33.0	40.0	22.0	41.0
48	33.72547002202397	37.0	33.0	40.0	22.0	41.0
49	33.64507026109454	37.0	33.0	40.0	21.0	41.0
50	33.36608363649265	36.0	32.0	40.0	20.0	41.0
51	31.496491721226246	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	4.0
11	3.0
12	7.0
13	11.0
14	12.0
15	25.0
16	60.0
17	112.0
18	226.0
19	503.0
20	795.0
21	1245.0
22	1776.0
23	2461.0
24	3114.0
25	3696.0
26	4558.0
27	5537.0
28	6810.0
29	8422.0
30	10645.0
31	13565.0
32	16857.0
33	22311.0
34	33538.0
35	37752.0
36	37118.0
37	48604.0
38	60972.0
39	45671.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.600239070572215	33.98459141037992	20.30789888078948	26.107270638258388
2	21.979482505001105	34.11640771903204	20.935595588656703	22.968514187310156
3	19.878063091706487	32.368135931815765	22.85307257538501	24.90072840109274
4	19.996779642977028	32.97181641781676	19.90535425291811	27.126049686288102
5	17.149492793768882	37.33567309555455	18.7501193988303	26.764714711846278
6	20.526228170482426	37.10833772266176	21.212600874954628	21.152833231901184
7	79.53517694224371	8.243295243969335	6.833160944165013	5.388366869621936
8	82.13657042893519	5.516362415704426	4.963716401169154	7.383350754191239
9	75.15194354004568	10.917829042707938	9.389524014857308	4.540703402389068
10	35.32076666330076	38.4041220569894	12.44313204282529	13.83197923688455
11	26.468605612700213	23.641514222788665	33.13829250120763	16.751587663303486
12	27.954063517448603	24.193068590875473	31.598525185648125	16.2543427060278
13	22.286508068631814	32.917779918617754	26.344976652411585	18.450735360338847
14	16.220228754513276	34.44935988581378	32.18610388653427	17.144307473138674
15	15.480092462454184	30.85265774973459	33.453778324813946	20.21347146299728
16	16.48959251567195	28.48869736558421	34.120228481601664	20.901481637142176
17	16.006538962226305	28.292473916472673	30.59202716016364	25.108959961137383
18	18.738384199509305	29.019783362762304	28.749600866767278	23.492231570961113
19	20.66459435782533	32.19238085361294	28.673185615374745	18.46983917318698
20	23.10688037465306	30.921158564375755	26.666739443096567	19.30522161787462
21	18.700449485425157	31.454973677675014	27.040628351695734	22.8039484852041
22	17.481080402490047	29.789939932154176	28.08669856093707	24.64228110441871
23	20.561433768445415	29.818868563038485	24.6488309831095	24.970866685406598
24	16.872760419083072	32.35121541186456	29.004773224095914	21.771250944956456
25	17.843234111768222	28.973115477090435	27.584814106255408	25.598836304885936
26	20.633482434044087	33.23572194673311	23.914698746516965	22.216096872705837
27	16.41890840813386	32.276983453368956	27.22457077826204	24.07953736023514
28	19.261828671548145	28.3503311782413	29.37020187271948	23.017638277491066
29	18.31919196329885	30.154549845941396	27.29934855998188	24.22690963077788
30	21.146283353210396	30.616316293641976	24.46352399848261	23.773876354665013
31	24.223088868208254	29.654848684156658	22.69860460292725	23.423457844707833
32	21.885873822045255	31.78983622574157	23.32220763661273	23.002082315600447
33	24.863612421844937	27.960067572915158	24.24110103460792	22.935218970631983
34	18.45892270870233	28.517353084856406	26.33706221566021	26.686661990781047
35	20.603462156711306	27.769575267658063	27.23002901050437	24.396933565126265
36	23.358231969412067	26.800193221421374	29.006683605380722	20.83489120378583
37	19.99377761524375	32.33948021254356	25.968358627691252	21.698383544521434
38	24.518106320905847	29.480458164014422	26.725688351313657	19.27574716376607
39	21.5392760746577	28.22315436699516	28.73158870036761	21.505980857979527
40	24.01021781075763	26.717773914562294	27.005695665344863	22.266312609335216
41	19.1649450492469	23.234057185899204	33.34215747545842	24.258840289395472
42	23.337490686891236	26.11791419113092	26.779724850512665	23.76487027146518
43	20.88483402880309	26.695668073980876	28.315125580278316	24.104372316937713
44	21.482510459337533	26.745337987386026	26.474336756554656	25.297814796721784
45	24.494090099039624	28.213602460571096	25.382144484865687	21.910162955523592
46	19.509359503737524	26.57067455563167	31.755722274227043	22.164243666403763
47	21.415101291144836	26.539016808626194	26.569037085958968	25.47684481427
48	21.819283388688905	22.944225053831815	33.151665170201326	22.084826387277953
49	21.587581430002263	22.467175555852727	31.014221424107376	24.931021590037634
50	19.71486194766101	25.31910190246685	26.539562631850423	28.426473518021716
51	18.952892726632626	25.103774640507183	31.006306987356	24.93702564550419
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2222.0
1	1779.5
2	1337.0
3	959.5
4	582.0
5	550.5
6	519.0
7	548.5
8	578.0
9	625.5
10	673.0
11	767.5
12	862.0
13	886.0
14	910.0
15	979.5
16	1049.0
17	1068.5
18	1088.0
19	1155.0
20	1222.0
21	1280.0
22	1338.0
23	1530.0
24	1722.0
25	1863.5
26	2880.5
27	3756.0
28	4266.5
29	4777.0
30	5366.0
31	5955.0
32	7226.0
33	8497.0
34	9236.5
35	9976.0
36	10943.0
37	11910.0
38	12873.5
39	13837.0
40	15884.5
41	17932.0
42	22841.5
43	27751.0
44	32488.5
45	37226.0
46	39160.5
47	41095.0
48	41659.0
49	42223.0
50	38667.5
51	35112.0
52	31350.5
53	27589.0
54	23126.0
55	18663.0
56	15775.5
57	12888.0
58	11222.0
59	9556.0
60	8300.0
61	7044.0
62	6374.5
63	5705.0
64	4883.5
65	4062.0
66	2940.5
67	1819.0
68	1372.5
69	926.0
70	770.5
71	615.0
72	560.5
73	506.0
74	447.5
75	336.5
76	284.0
77	196.0
78	108.0
79	78.0
80	48.0
81	36.5
82	25.0
83	18.5
84	12.0
85	10.5
86	9.0
87	6.5
88	4.0
89	4.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.16939246307613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94949793008517	29.716742657892283
2	8.47273383147708	6.298527380347087
3	2.741393004901702	3.056877374841688
4	1.37124354058449	2.038731572897719
5	0.8420414458838441	1.5649084486116338
6	0.5854806070935987	1.3057175078749226
7	0.4428120123505718	1.1521337432096042
8	0.3972615404560036	1.1812776086156316
9	0.33273557619790345	1.113082129631488
>10	4.451461466254461	38.218785232390445
>50	0.37376402979562445	8.529500486867624
>100	0.0344449203929301	2.440463555884231
>500	0.0014657412933161743	0.4116017895904758
>1k	0.003664353233290436	2.971650511345136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	2957	0.806999637027556	TruSeq Adapter, Index 15 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	2273	0.6203280943400861	No Hit
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1935	0.528083969444816	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1911	0.5215340907540276	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT	1833	0.5002469850089651	No Hit
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	902	0.24616627412879794	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	609	0.16620317177875601	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	461	0.1258122531855608	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	445	0.12144566739170186	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.307246622036521	0.0
2	0.0	0.0	0.0	1.6456570210605892	0.0
3	0.0	0.0	0.0	2.167464023426733	0.0
4	0.0	0.0	0.0	5.062783316367328	0.0
5	0.0	0.0	0.0	5.438036783027081	0.0
6	0.0	0.0	0.0	7.262723821635887	0.0
7	0.0	0.0	0.0	8.726894620639214	0.0
8	0.0	0.0	0.0	9.638146493495151	0.0
9	0.0	0.0	0.0	11.678160794063627	0.0
10	0.0	0.0	0.0	13.55306356930181	0.0
11	0.0	0.0	0.0	16.24861156217336	0.0
12	0.0	0.0	0.0	17.25838452700324	0.0
13	0.0	0.0	0.0	17.672118530971375	0.0
14	0.0	0.0	0.0	18.16035740504723	0.0
15	0.0	0.0	0.0	18.51050300339229	0.0
16	0.0	0.0	0.0	19.181865569198102	0.0
17	0.0	0.0	0.0	20.089296679484416	0.0
18	0.0	0.0	0.0	21.137004358398446	0.0
19	0.0	0.0	0.0	21.638888813080108	0.0
20	0.0	0.0	0.0	22.09656158659895	0.0
21	0.0	0.0	0.0	22.64838886629787	0.0
22	0.0	0.0	0.0	23.276631397389327	0.0
23	0.0	0.0	0.0	23.904055193644435	0.0
24	0.0	0.0	0.0	24.3284327504851	0.0
25	0.0	0.0	0.0	24.70532368681755	0.0
26	0.0	0.0	0.0	25.027086477502532	0.0
27	0.0	0.0	0.0	25.38050701519299	0.0
28	0.0	0.0	0.0	25.74593566381656	0.0
29	0.0	0.0	0.0	26.141930412997144	0.0
30	0.0	0.0	0.0	26.64927309992113	0.0
31	0.0	0.0	0.0	27.040628351695737	0.0
32	0.0	0.0	0.0	27.37630963459864	0.0
33	0.0	0.0	0.0	27.72700105616794	0.0
34	0.0	0.0	0.0	28.0703238642101	0.0
35	0.0	0.0	0.0	28.47805381271168	0.0
36	0.0	0.0	0.0	28.839388787153503	0.0
37	0.0	0.0	0.0	29.207546551898236	0.0
38	0.0	0.0	0.0	29.550323536716164	0.0
39	0.0	0.0	0.0	29.915479273727616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	20	7.0269883E-4	45.000004	6
CGAACTT	20	7.0269883E-4	45.000004	21
TACCCGG	35	1.2089004E-7	45.000004	2
GCAAGCG	20	7.0269883E-4	45.000004	1
CCCTCGG	20	7.0269883E-4	45.000004	2
GTCGATA	20	7.0269883E-4	45.000004	37
CGTGCGC	20	7.0269883E-4	45.000004	17
CGCATAG	30	2.1614396E-6	45.000004	1
GGATCGA	20	7.0269883E-4	45.000004	8
TCGATAT	20	7.0269883E-4	45.000004	38
CACCTAG	20	7.0269883E-4	45.000004	1
ACGATTG	20	7.0269883E-4	45.000004	1
AGTGCGG	40	6.7939254E-9	45.000004	2
GTAGCGG	40	6.7939254E-9	45.000004	2
GCCGAGT	20	7.0269883E-4	45.000004	20
AAGCCCG	20	7.0269883E-4	45.000004	1
TGCGCGA	20	7.0269883E-4	45.000004	19
CCATCGA	20	7.0269883E-4	45.000004	10
CTCGGTA	20	7.0269883E-4	45.000004	22
CATAGCG	30	2.1614396E-6	45.000004	1
>>END_MODULE