FastQCFastQC Report
Sat 18 Jun 2016
SRR3548847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373536
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT28400.7603015505868242TruSeq Adapter, Index 15 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC23240.6221622547759788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19560.5236443073759959No Hit
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT17860.4781332990662212No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT17230.4612674548102459No Hit
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT8320.22273622890430908No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5430.1453675147776921No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT5110.136800736742911No Hit
AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT4530.12127345155487021No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT3810.10199820097661269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG207.027164E-445.0000041
CAGCGCG207.027164E-445.0000041
TCTTGCG302.1615524E-645.0000041
ACCGGTA207.027164E-445.00000427
GTTCTCG207.027164E-445.00000444
GGTCGGG302.1615524E-645.0000043
ACCGCGC207.027164E-445.00000410
CGGCATT207.027164E-445.00000426
GGGTACG351.2089913E-745.0000047
ATTACCG207.027164E-445.0000041
TAAACGG406.7939254E-945.0000042
AAGGTGC207.027164E-445.00000442
AGGGTAC750.045.0000046
CGCTGTT207.027164E-445.00000430
CGATTAA207.027164E-445.00000424
TCTAGCG207.027164E-445.0000041
GTCAGAC302.1615524E-645.00000420
GTAGCGG406.7939254E-945.0000042
GAGACGA207.027164E-445.0000048
TCCAGCG207.027164E-445.0000041