##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373536
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08915606527885	31.0	31.0	34.0	28.0	34.0
2	31.302950719609356	31.0	31.0	34.0	28.0	34.0
3	31.282885719181017	31.0	31.0	34.0	28.0	34.0
4	35.31530829692453	37.0	35.0	37.0	33.0	37.0
5	34.97482170393215	35.0	35.0	37.0	32.0	37.0
6	35.026621262743085	36.0	35.0	37.0	32.0	37.0
7	35.0583129872355	37.0	35.0	37.0	32.0	37.0
8	35.433173777092435	37.0	35.0	37.0	33.0	37.0
9	35.8374882206802	39.0	37.0	39.0	32.0	39.0
10	35.509369913475545	37.0	35.0	39.0	30.0	39.0
11	36.006366186927096	37.0	35.0	39.0	31.0	39.0
12	35.93968185128073	37.0	35.0	39.0	31.0	39.0
13	35.741901717638996	37.0	35.0	39.0	30.0	39.0
14	36.77260558553928	38.0	35.0	40.0	31.0	41.0
15	36.83721783174848	38.0	35.0	40.0	31.0	41.0
16	36.82366358262657	38.0	35.0	40.0	31.0	41.0
17	36.81228583054913	38.0	35.0	40.0	32.0	41.0
18	36.66771609697593	38.0	35.0	40.0	31.0	41.0
19	36.71204114195151	38.0	35.0	40.0	31.0	41.0
20	36.657154865929925	38.0	35.0	40.0	31.0	41.0
21	36.67999068362889	38.0	35.0	40.0	31.0	41.0
22	36.714747708386874	38.0	35.0	40.0	31.0	41.0
23	36.65391019874925	38.0	35.0	40.0	31.0	41.0
24	36.24172770496016	38.0	35.0	40.0	30.0	41.0
25	36.39011768611325	38.0	35.0	40.0	30.0	41.0
26	36.25662854450441	38.0	35.0	40.0	30.0	41.0
27	36.173653945001284	38.0	35.0	40.0	30.0	41.0
28	36.12992054313373	38.0	35.0	40.0	30.0	41.0
29	35.9325071746766	38.0	35.0	40.0	30.0	41.0
30	35.864320975756016	38.0	34.0	40.0	29.0	41.0
31	35.735289235843396	38.0	34.0	40.0	29.0	41.0
32	35.621211877837744	38.0	34.0	40.0	29.0	41.0
33	35.61977158828065	38.0	34.0	40.0	29.0	41.0
34	35.47245513150004	38.0	34.0	40.0	28.0	41.0
35	35.449402467232076	38.0	34.0	40.0	28.0	41.0
36	35.292748222393556	38.0	34.0	40.0	27.0	41.0
37	35.039396470487446	38.0	34.0	40.0	26.0	41.0
38	35.05338441274736	38.0	34.0	40.0	26.0	41.0
39	34.812671335560694	37.0	33.0	40.0	25.0	41.0
40	34.66729578942859	37.0	33.0	40.0	25.0	41.0
41	34.66061370256147	37.0	33.0	40.0	24.0	41.0
42	34.69021727490791	37.0	33.0	40.0	24.0	41.0
43	34.67122847597019	37.0	33.0	40.0	24.0	41.0
44	34.58978518804078	37.0	33.0	40.0	24.0	41.0
45	34.294881885547845	37.0	33.0	40.0	23.0	41.0
46	34.0830174333933	37.0	33.0	40.0	23.0	41.0
47	33.93771149233273	37.0	33.0	40.0	23.0	41.0
48	33.82776225049259	37.0	33.0	40.0	22.0	41.0
49	33.7683302278763	37.0	33.0	40.0	22.0	41.0
50	33.6264028099032	36.0	32.0	40.0	22.0	41.0
51	31.87816703075473	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	4.0
12	5.0
13	6.0
14	14.0
15	24.0
16	57.0
17	145.0
18	305.0
19	499.0
20	852.0
21	1334.0
22	1794.0
23	2401.0
24	3065.0
25	3688.0
26	4464.0
27	5424.0
28	7053.0
29	8669.0
30	10826.0
31	13784.0
32	17688.0
33	23327.0
34	34655.0
35	39096.0
36	39128.0
37	50581.0
38	61626.0
39	43014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.81094191724492	33.62219438019361	20.3554677460807	26.211395956480764
2	22.20321468345755	33.866079842371285	20.87670264713441	23.05400282703675
3	20.263107170393216	32.29354064936177	22.613616893686284	24.829735286558723
4	20.019489420029128	33.11140023986979	19.776139381478625	27.092970958622466
5	17.28749036237471	37.328664439304376	18.356463634027243	27.027381564293666
6	20.34342071446929	37.24487064165167	21.24614494988435	21.165563693994688
7	80.2723164567806	8.226784031525742	6.335667780347812	5.165231731345841
8	82.84797181530027	5.515934207144693	4.703428852908422	6.93266512464662
9	74.95823695708043	12.018118735543561	8.725531140238155	4.298113167137839
10	35.89372911847854	38.5818234387047	12.262004197721236	13.26244324509552
11	26.718709843227963	23.60174119763557	32.906868414289384	16.77268054484708
12	28.277060310117363	24.29993360747023	31.24143322196522	16.181572860447186
13	22.439336503041208	32.9802214512122	26.414053799366062	18.166388246380535
14	16.404309089351496	34.616476055855394	31.74874710871241	17.2304677460807
15	15.677739227276621	30.616058425426196	33.50118864045233	20.205013706844856
16	16.584746851709074	28.394050372654846	34.348496530454895	20.67270624518119
17	16.265634369913474	27.99837231217339	30.476848282361	25.259145035552127
18	19.028420286130387	28.89520688768954	29.010055255718324	23.06631757046175
19	20.965850681058853	31.912586738627603	28.654801679088493	18.466760901225047
20	23.243542791056285	30.92151760472886	26.69729289814101	19.137646706073845
21	18.939272252205946	31.33004583226249	27.394682172534914	22.33599974299666
22	17.87404694594363	29.63917801764756	27.805887518204404	24.680887518204404
23	20.40124646620406	30.164964019532253	24.776728347468516	24.65706116679517
24	17.1445322539193	32.059025100659646	29.25260215882807	21.54384048659299
25	17.851291441788746	29.33184485564979	27.628126873982694	25.188736828578772
26	20.498693566349697	33.485125931637114	24.069701447785487	21.946479054227705
27	16.420104086353124	32.643439989719866	27.426004454724577	23.510451469202433
28	19.13095391073417	28.70700548273794	29.695932922127987	22.466107684399898
29	18.458729546817445	30.22867943116594	27.59385976184357	23.718731260173048
30	21.22419258117022	30.6712070590251	24.694808532510923	23.409791827293756
31	24.036237471087123	30.145688768954	22.779865929923755	23.038207830035123
32	22.2492611153945	31.562955110083095	23.570151203632314	22.617632570890088
33	24.580495588109315	28.376381392958113	24.345712327593592	22.697410691338987
34	18.59392401267883	28.656675661783602	26.259316371112824	26.490083954424744
35	20.68930437762358	27.93519232416688	27.21424441017733	24.16125888803221
36	23.30538421999486	27.200591107684403	28.64891201918958	20.845112653131157
37	20.03314272252206	32.64049515977041	25.96001456352266	21.366347554184873
38	24.282264627773493	29.587777349438877	26.627955538422	19.50200248436563
39	21.496990919215282	28.7201233616037	28.347736228904306	21.435149490276707
40	24.12833033496102	26.63571918101602	27.058168422856166	22.177782061166795
41	19.009680459179304	23.712306176646962	32.876349267540476	24.401664096633255
42	23.133245523858477	26.42074659470573	26.737449670179046	23.708558211256747
43	20.778452411548017	26.823385162340447	28.429656043861907	23.968506382249636
44	21.265420200462607	27.334447014477853	26.678820783003516	24.721312002056024
45	24.413711128244667	28.482127559324937	25.363284931037438	21.740876381392958
46	19.548584339929754	26.98401225049259	31.538057911419514	21.929345498158142
47	21.41908678146149	27.027381564293666	26.489816242611152	25.063715411633687
48	21.838323481538595	23.30377794911334	32.998693566349694	21.859205002998372
49	21.690011136811446	22.987878009080784	30.696371969502266	24.6257388846055
50	19.788454124903623	25.790820697335732	26.424762271909536	27.99596290585111
51	19.0343099460293	25.70488520517433	30.51700505439904	24.74379979439733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2291.0
1	1879.0
2	1467.0
3	1010.5
4	554.0
5	539.0
6	524.0
7	535.0
8	546.0
9	639.5
10	733.0
11	811.0
12	889.0
13	890.5
14	892.0
15	969.5
16	1047.0
17	1055.0
18	1063.0
19	1165.5
20	1268.0
21	1372.0
22	1476.0
23	1614.5
24	1753.0
25	1917.0
26	3052.0
27	4023.0
28	4510.5
29	4998.0
30	5682.0
31	6366.0
32	7538.5
33	8711.0
34	9610.0
35	10509.0
36	11493.5
37	12478.0
38	13542.0
39	14606.0
40	16890.5
41	19175.0
42	23872.0
43	28569.0
44	33131.5
45	37694.0
46	39510.5
47	41327.0
48	41485.5
49	41644.0
50	38340.0
51	35036.0
52	31117.5
53	27199.0
54	23121.5
55	19044.0
56	16206.0
57	13368.0
58	11664.5
59	9961.0
60	8665.0
61	7369.0
62	6607.5
63	5846.0
64	4960.0
65	4074.0
66	3017.5
67	1961.0
68	1481.5
69	1002.0
70	811.5
71	621.0
72	566.5
73	512.0
74	434.5
75	327.0
76	297.0
77	188.0
78	79.0
79	69.5
80	60.0
81	40.5
82	21.0
83	18.0
84	15.0
85	16.0
86	17.0
87	12.0
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.13268307696912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76674818990647	29.619533806161975
2	8.632913204562362	6.411264601119922
3	2.884718772973113	3.213520438889815
4	1.373560922110263	2.040160096305195
5	0.7938356459324596	1.4738623727805547
6	0.5683862213568239	1.2663423253775377
7	0.46139743928501015	1.1993047419846767
8	0.41057136596703636	1.2196493130345814
9	0.3084408845560209	1.030791385277887
>10	4.362853107038702	37.78899620922249
>50	0.39385802652336194	8.990883298756886
>100	0.03692418998656518	2.3811336715333473
>500	0.002172011175680305	0.5070360285098672
>1k	0.003620018626133841	2.857521711045269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	2840	0.7603015505868242	TruSeq Adapter, Index 15 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	2324	0.6221622547759788	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1956	0.5236443073759959	No Hit
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1786	0.4781332990662212	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT	1723	0.4612674548102459	No Hit
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	832	0.22273622890430908	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	543	0.1453675147776921	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	511	0.136800736742911	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	453	0.12127345155487021	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	381	0.10199820097661269	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1961363831063136	0.0
2	0.0	0.0	0.0	1.5007924269682174	0.0
3	0.0	0.0	0.0	1.9746423370170478	0.0
4	0.0	0.0	0.0	4.497290756446501	0.0
5	0.0	0.0	0.0	4.825773151717639	0.0
6	0.0	0.0	0.0	6.471397669836374	0.0
7	0.0	0.0	0.0	7.782114709157885	0.0
8	0.0	0.0	0.0	8.611485907650133	0.0
9	0.0	0.0	0.0	10.483326908249808	0.0
10	0.0	0.0	0.0	12.285295125503298	0.0
11	0.0	0.0	0.0	14.908067763214255	0.0
12	0.0	0.0	0.0	15.874239698449413	0.0
13	0.0	0.0	0.0	16.27982309603358	0.0
14	0.0	0.0	0.0	16.7252955538422	0.0
15	0.0	0.0	0.0	17.05002998372312	0.0
16	0.0	0.0	0.0	17.71047502784203	0.0
17	0.0	0.0	0.0	18.57491647391416	0.0
18	0.0	0.0	0.0	19.607213227105287	0.0
19	0.0	0.0	0.0	20.07918915445901	0.0
20	0.0	0.0	0.0	20.531622119420884	0.0
21	0.0	0.0	0.0	21.05446329135612	0.0
22	0.0	0.0	0.0	21.66698792084297	0.0
23	0.0	0.0	0.0	22.26211128244667	0.0
24	0.0	0.0	0.0	22.684560524286816	0.0
25	0.0	0.0	0.0	23.038743253662297	0.0
26	0.0	0.0	0.0	23.359729718153	0.0
27	0.0	0.0	0.0	23.703471686798594	0.0
28	0.0	0.0	0.0	24.05818983980125	0.0
29	0.0	0.0	0.0	24.42656129529684	0.0
30	0.0	0.0	0.0	24.914332219652188	0.0
31	0.0	0.0	0.0	25.288861046860276	0.0
32	0.0	0.0	0.0	25.61680801850424	0.0
33	0.0	0.0	0.0	25.949038379165597	0.0
34	0.0	0.0	0.0	26.283410434335646	0.0
35	0.0	0.0	0.0	26.692206373682858	0.0
36	0.0	0.0	0.0	27.052814186584424	0.0
37	0.0	0.0	0.0	27.414225134926753	0.0
38	0.0	0.0	0.0	27.758770239013106	0.0
39	0.0	0.0	0.0	28.105992461235328	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	20	7.027164E-4	45.000004	1
CAGCGCG	20	7.027164E-4	45.000004	1
TCTTGCG	30	2.1615524E-6	45.000004	1
ACCGGTA	20	7.027164E-4	45.000004	27
GTTCTCG	20	7.027164E-4	45.000004	44
GGTCGGG	30	2.1615524E-6	45.000004	3
ACCGCGC	20	7.027164E-4	45.000004	10
CGGCATT	20	7.027164E-4	45.000004	26
GGGTACG	35	1.2089913E-7	45.000004	7
ATTACCG	20	7.027164E-4	45.000004	1
TAAACGG	40	6.7939254E-9	45.000004	2
AAGGTGC	20	7.027164E-4	45.000004	42
AGGGTAC	75	0.0	45.000004	6
CGCTGTT	20	7.027164E-4	45.000004	30
CGATTAA	20	7.027164E-4	45.000004	24
TCTAGCG	20	7.027164E-4	45.000004	1
GTCAGAC	30	2.1615524E-6	45.000004	20
GTAGCGG	40	6.7939254E-9	45.000004	2
GAGACGA	20	7.027164E-4	45.000004	8
TCCAGCG	20	7.027164E-4	45.000004	1
>>END_MODULE