SRR3548846_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322142
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	6714	2.084174059886634	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	6483	2.0124665520174334	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGT	5864	1.820315264696935	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	3707	1.1507347691390752	TruSeq Adapter, Index 13 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2423	0.752152777346636	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	1454	0.4513537508303791	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	1292	0.40106536868834236	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	1201	0.37281695649744523	No Hit
AACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	1062	0.32966828293112976	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	962	0.2986260717323416	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTAT	645	0.2002222622321833	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	605	0.1878053777526681	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	586	0.18190735762489835	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	483	0.14993388009014658	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.14869219164219505	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	477	0.14807134741821928	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCCACTATCGTATGCC	408	0.1266522216910555	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	406	0.12603137746707974	No Hit
TATTGAGGGTCTCTTCCTTCACATCCACGTCATACAGCAGGTCGGACTCAC	350	0.1086477391957584	No Hit
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	325	0.10088718639606137	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.0257176E-4	45.000004	28
CTTCGGA	20	7.0257176E-4	45.000004	13
CCCTCTA	20	7.0257176E-4	45.000004	14
GTCCTTC	20	7.0257176E-4	45.000004	40
GCTATTT	20	7.0257176E-4	45.000004	36
CCCAGCG	35	1.2082819E-7	45.000004	1
TTCAATG	20	7.0257176E-4	45.000004	12
GGTTTAG	20	7.0257176E-4	45.000004	9
CCGCTTG	20	7.0257176E-4	45.000004	27
TGCGGGT	40	6.7884685E-9	45.000004	4
TGCGGGC	35	1.2082819E-7	45.000004	4
CGACAAT	40	6.7884685E-9	45.000004	20
TGTGCGG	20	7.0257176E-4	45.000004	2
CGTTCCG	20	7.0257176E-4	45.000004	1
ATACTAT	30	2.1606193E-6	45.000004	45
ATATCGG	20	7.0257176E-4	45.000004	45
CTAACGG	40	6.7884685E-9	45.000004	2
CGCTCCG	20	7.0257176E-4	45.000004	1
CGCTATT	20	7.0257176E-4	45.000004	35
TGCGATT	20	7.0257176E-4	45.000004	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content