##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322142
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14782921817087	31.0	31.0	34.0	28.0	34.0
2	31.306659795990587	31.0	31.0	34.0	28.0	34.0
3	31.505435491180908	31.0	31.0	34.0	30.0	34.0
4	35.35910561181094	37.0	35.0	37.0	33.0	37.0
5	34.88394558921221	35.0	35.0	37.0	32.0	37.0
6	34.86724798380838	35.0	35.0	37.0	32.0	37.0
7	34.802105903607725	35.0	35.0	37.0	32.0	37.0
8	35.19139075314613	37.0	35.0	37.0	32.0	37.0
9	36.08364944651738	39.0	35.0	39.0	32.0	39.0
10	35.691437316462924	37.0	35.0	39.0	30.0	39.0
11	35.99353701162841	37.0	35.0	39.0	31.0	39.0
12	35.51957521838196	37.0	35.0	39.0	30.0	39.0
13	35.172694029341095	37.0	34.0	39.0	30.0	39.0
14	36.097236622359084	38.0	34.0	40.0	30.0	41.0
15	36.278029564601944	38.0	35.0	40.0	31.0	41.0
16	36.27522024448846	38.0	35.0	40.0	31.0	41.0
17	36.251339471413225	38.0	35.0	40.0	31.0	41.0
18	35.96558660466502	37.0	35.0	40.0	30.0	41.0
19	35.91377715417425	37.0	34.0	40.0	30.0	41.0
20	35.94804775533771	37.0	34.0	40.0	30.0	41.0
21	36.05151765370551	37.0	34.0	40.0	30.0	41.0
22	36.02708432927094	37.0	34.0	40.0	30.0	41.0
23	35.928373201879914	37.0	34.0	40.0	30.0	41.0
24	35.565731882213434	37.0	34.0	40.0	29.0	41.0
25	35.60127210981493	37.0	34.0	40.0	29.0	41.0
26	35.48385805017663	37.0	34.0	40.0	29.0	41.0
27	35.40200594768766	37.0	34.0	40.0	29.0	41.0
28	35.28886640053144	37.0	34.0	40.0	27.0	41.0
29	35.139733409490226	37.0	34.0	40.0	27.0	41.0
30	35.130414537688345	37.0	34.0	40.0	27.0	41.0
31	34.65598400705279	36.0	33.0	40.0	25.0	41.0
32	34.56459263306244	36.0	33.0	40.0	25.0	41.0
33	34.646149834545014	37.0	33.0	40.0	25.0	41.0
34	34.49381328730808	37.0	33.0	40.0	25.0	41.0
35	34.38934072551856	36.0	33.0	40.0	25.0	41.0
36	34.16712195243092	36.0	33.0	40.0	24.0	41.0
37	34.01928342159669	36.0	33.0	40.0	23.0	41.0
38	33.804219878190366	36.0	33.0	39.0	23.0	41.0
39	33.73853145507261	36.0	33.0	39.0	23.0	41.0
40	33.750454768394064	36.0	33.0	39.0	23.0	41.0
41	33.89027509607564	36.0	33.0	39.0	23.0	41.0
42	33.803117879692806	36.0	33.0	39.0	23.0	41.0
43	33.72367775701399	36.0	33.0	39.0	23.0	41.0
44	33.551455569283114	36.0	33.0	39.0	22.0	40.0
45	33.21520323335672	35.0	32.0	39.0	21.0	40.0
46	33.106127111646416	35.0	32.0	39.0	21.0	40.0
47	33.108191418691135	35.0	32.0	39.0	20.0	40.0
48	33.11170850121996	35.0	32.0	39.0	20.0	40.0
49	33.00181596935513	35.0	32.0	39.0	20.0	40.0
50	32.73905606844187	35.0	31.0	39.0	19.0	40.0
51	30.56429773205605	34.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	9.0
12	10.0
13	6.0
14	10.0
15	22.0
16	59.0
17	168.0
18	351.0
19	668.0
20	1157.0
21	1702.0
22	2375.0
23	3160.0
24	3540.0
25	4113.0
26	4652.0
27	5459.0
28	6662.0
29	8415.0
30	10641.0
31	13557.0
32	17627.0
33	23181.0
34	36505.0
35	40270.0
36	32338.0
37	38429.0
38	41256.0
39	25788.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.99995654090432	40.464143141844275	18.789229594402467	23.746670722848933
2	17.40723035183242	41.9159873596116	18.620049543369074	22.056732745186906
3	18.1224428978525	31.31941814479329	27.46521720235176	23.09292175500245
4	23.2248511525973	32.29879990811506	18.445281894319894	26.03106704496775
5	15.021636421205557	41.47518796058881	18.783952418498675	24.71922319970696
6	19.54821165821284	35.028031116712505	24.836873180150366	20.58688404492429
7	70.01384482619466	14.578043223174872	8.939535981027001	6.468575969603467
8	72.5226763352807	8.078735464484605	6.2233425011330405	13.17524569910164
9	68.82523855939307	12.413469836283378	12.323447423806892	6.437844180516667
10	38.01708563304381	33.21578682692726	13.11968014105581	15.647447398973124
11	31.27347567221908	22.802056236069806	28.022735315481995	17.901732776229114
12	29.468060668897568	23.232611705396998	32.568246301320535	14.731081324384899
13	21.297750681376538	38.00001241688448	22.541301661999988	18.160935239738997
14	12.297061544287923	38.167329935245945	34.253217525190756	15.282390995275374
15	11.34375523837314	31.219462224733192	35.53681295826065	21.899969578633023
16	12.625798560883089	26.71461653556506	38.63079014844386	22.028794755108
17	12.65497823940995	27.84113837996908	29.02477789297887	30.479105487642094
18	16.026472797710326	28.80065312812362	29.729125665079376	25.443748409086673
19	18.359915813523227	33.895611252180714	29.366552638277533	18.377920296018527
20	21.710612090320417	33.24900199290996	24.59505435491181	20.445331561857813
21	15.864742877364641	33.574013944161265	25.843572089327065	24.71767108914702
22	15.755784716056894	33.12483314811481	26.503529499413304	24.615852636415
23	19.769542624060197	32.033078580253424	20.132736495086018	28.064642300600358
24	14.364783232239198	36.62297992810623	28.065263144824332	20.94697369483023
25	15.15294497457643	28.375064412588237	28.886640053144262	27.58535055969107
26	20.44750451664173	36.7161065617026	20.844844819986218	21.99154410166945
27	13.199148201724705	32.85321380012541	26.897455159525922	27.05018283862396
28	17.18310558697717	29.00304834513972	28.282869045327836	25.53097702255527
29	19.273488089103562	30.6377311868679	28.667171619968833	21.421609104059698
30	18.828032358400954	29.042161531250194	22.02848433299601	30.101321777352847
31	25.113459281931572	34.72878420075619	18.080225490622148	22.077531026690092
32	22.37864047531834	33.24434566123014	17.64904917707098	26.72796468638054
33	22.415891128756886	28.293112975023437	20.11317990203078	29.1778159941889
34	18.285414506646138	29.13808196385445	24.67234946079679	27.90415406870262
35	16.403014819551625	30.829882474188402	22.46586908878693	30.301233617473038
36	24.362237770920895	26.90987204400544	27.362777905395756	21.365112279677906
37	16.96332673168975	33.669003110429564	21.967331176934398	27.400338980946294
38	19.554730522564583	34.306920550564655	22.952921382495916	23.18542754437484
39	16.5138355135313	31.18748874719844	29.262871652873578	23.035804086396684
40	20.313402164262964	30.581855206710085	21.61469165771616	27.490050971310787
41	16.842572530126464	22.46617951089892	34.478273556381964	26.21297440259265
42	23.88387729634757	28.157148089972743	22.62915111969256	25.329823493987124
43	20.38045334045235	24.616473480638973	27.806991947650417	27.196081231258262
44	19.674863879903892	25.12960123175494	26.069869808966235	29.125665079374937
45	24.525209379714536	30.93263219325639	22.048351348163234	22.493807078865842
46	18.06222100812685	26.698785007853683	34.02381558443171	21.21517839958776
47	21.067417474281527	26.846235511047922	23.633056229861367	28.453290784809184
48	20.333579601542176	20.941386096814448	36.12164821724581	22.603386084397563
49	21.623073054739837	20.58595277858832	30.809084192685216	26.98188997398663
50	18.986347635514772	23.691726009027075	25.0563416133258	32.265584742132354
51	16.690776117364393	24.527071912386464	32.71383427184285	26.06831769840629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2853.0
1	2317.5
2	1782.0
3	1303.0
4	824.0
5	846.0
6	868.0
7	951.5
8	1035.0
9	1165.0
10	1295.0
11	1418.0
12	1541.0
13	1564.5
14	1588.0
15	1635.0
16	1682.0
17	1647.0
18	1612.0
19	1519.0
20	1426.0
21	1405.5
22	1385.0
23	1437.5
24	1490.0
25	1578.0
26	1879.0
27	2092.0
28	2261.5
29	2431.0
30	2714.5
31	2998.0
32	3693.5
33	4389.0
34	5173.0
35	5957.0
36	6317.5
37	6678.0
38	7812.0
39	8946.0
40	10817.5
41	12689.0
42	17726.0
43	22763.0
44	34527.0
45	46291.0
46	45656.5
47	45022.0
48	41687.0
49	38352.0
50	34579.5
51	30807.0
52	26575.0
53	22343.0
54	19159.0
55	15975.0
56	13386.5
57	10798.0
58	8896.5
59	6995.0
60	5978.5
61	4962.0
62	4279.5
63	3597.0
64	3088.5
65	2580.0
66	2079.0
67	1578.0
68	1326.0
69	1074.0
70	787.0
71	500.0
72	432.0
73	364.0
74	322.0
75	287.5
76	295.0
77	234.5
78	174.0
79	111.5
80	49.0
81	51.0
82	53.0
83	41.0
84	29.0
85	23.0
86	17.0
87	12.5
88	8.0
89	6.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.84469489081033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16664936002894	25.345150539762944
2	10.342163627160048	6.79370417689816
3	3.537587320549409	3.485729285791337
4	1.7526205948540632	2.302571547893288
5	1.0146266134780793	1.666255077389183
6	0.7145194188669859	1.4080903383747154
7	0.5281526220211756	1.214290820824689
8	0.3866457889162127	1.015941037421573
9	0.3019422101022969	0.8925479788920202
>10	3.4857876761993882	25.862939592749058
>50	0.6766294525286317	14.606756179714358
>100	0.07972070132885205	4.605769314352883
>500	0.003986035066442602	0.9157861385417401
>1k	0.005979052599663903	3.6457976401774275
>5k	0.0029895262998319515	6.238670331216622
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	6714	2.084174059886634	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	6483	2.0124665520174334	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGT	5864	1.820315264696935	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	3707	1.1507347691390752	TruSeq Adapter, Index 13 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2423	0.752152777346636	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	1454	0.4513537508303791	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	1292	0.40106536868834236	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	1201	0.37281695649744523	No Hit
AACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	1062	0.32966828293112976	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	962	0.2986260717323416	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTAT	645	0.2002222622321833	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	605	0.1878053777526681	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	586	0.18190735762489835	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	483	0.14993388009014658	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.14869219164219505	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	477	0.14807134741821928	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCCACTATCGTATGCC	408	0.1266522216910555	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	406	0.12603137746707974	No Hit
TATTGAGGGTCTCTTCCTTCACATCCACGTCATACAGCAGGTCGGACTCAC	350	0.1086477391957584	No Hit
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	325	0.10088718639606137	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5632857559709694	0.0
2	0.0	0.0	0.0	1.9131314761813114	0.0
3	0.0	0.0	0.0	2.7279895201494995	0.0
4	0.0	0.0	0.0	7.362591652128565	0.0
5	0.0	0.0	0.0	7.936251715082169	0.0
6	0.0	0.0	0.0	10.41186805818552	0.0
7	0.0	0.0	0.0	12.487660721048481	0.0
8	0.0	0.0	0.0	13.72872832477603	0.0
9	0.0	0.0	0.0	17.42958074389555	0.0
10	0.0	0.0	0.0	19.738189990749422	0.0
11	0.0	0.0	0.0	24.421528394310585	0.0
12	0.0	0.0	0.0	25.67159823928578	0.0
13	0.0	0.0	0.0	26.28685486524576	0.0
14	0.0	0.0	0.0	26.9902713710103	0.0
15	0.0	0.0	0.0	27.391026317586654	0.0
16	0.0	0.0	0.0	28.13448727579763	0.0
17	0.0	0.0	0.0	28.98597512898039	0.0
18	0.0	0.0	0.0	30.26336211981052	0.0
19	0.0	0.0	0.0	30.745137237615708	0.0
20	0.0	0.0	0.0	31.2576441445077	0.0
21	0.0	0.0	0.0	31.776669915751437	0.0
22	0.0	0.0	0.0	32.348157023921125	0.0
23	0.0	0.0	0.0	32.914987800411	0.0
24	0.0	0.0	0.0	33.287494334796456	0.0
25	0.0	0.0	0.0	33.61530008505566	0.0
26	0.0	0.0	0.0	33.90026758386053	0.0
27	0.0	0.0	0.0	34.26532398755828	0.0
28	0.0	0.0	0.0	34.59250889359351	0.0
29	0.0	0.0	0.0	34.95787571940325	0.0
30	0.0	0.0	0.0	35.46169080715958	0.0
31	0.0	0.0	0.0	35.81588243693775	0.0
32	0.0	0.0	0.0	36.12568370470165	0.0
33	0.0	0.0	0.0	36.44821227905706	0.0
34	0.0	0.0	0.0	36.75925523526892	0.0
35	0.0	0.0	0.0	37.124622061078654	0.0
36	3.1042211198788113E-4	0.0	0.0	37.4282148866028	0.0
37	3.1042211198788113E-4	0.0	0.0	37.72653053622316	0.0
38	3.1042211198788113E-4	0.0	0.0	38.02546703006749	0.0
39	3.1042211198788113E-4	0.0	0.0	38.32905985559163	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.0257176E-4	45.000004	28
CTTCGGA	20	7.0257176E-4	45.000004	13
CCCTCTA	20	7.0257176E-4	45.000004	14
GTCCTTC	20	7.0257176E-4	45.000004	40
GCTATTT	20	7.0257176E-4	45.000004	36
CCCAGCG	35	1.2082819E-7	45.000004	1
TTCAATG	20	7.0257176E-4	45.000004	12
GGTTTAG	20	7.0257176E-4	45.000004	9
CCGCTTG	20	7.0257176E-4	45.000004	27
TGCGGGT	40	6.7884685E-9	45.000004	4
TGCGGGC	35	1.2082819E-7	45.000004	4
CGACAAT	40	6.7884685E-9	45.000004	20
TGTGCGG	20	7.0257176E-4	45.000004	2
CGTTCCG	20	7.0257176E-4	45.000004	1
ATACTAT	30	2.1606193E-6	45.000004	45
ATATCGG	20	7.0257176E-4	45.000004	45
CTAACGG	40	6.7884685E-9	45.000004	2
CGCTCCG	20	7.0257176E-4	45.000004	1
CGCTATT	20	7.0257176E-4	45.000004	35
TGCGATT	20	7.0257176E-4	45.000004	33
>>END_MODULE