##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323121
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95662925034275	31.0	31.0	33.0	28.0	34.0
2	31.156563640246222	31.0	31.0	34.0	28.0	34.0
3	31.15875786470084	31.0	31.0	34.0	28.0	34.0
4	35.164068568740504	37.0	35.0	37.0	32.0	37.0
5	34.769906629405085	35.0	35.0	37.0	32.0	37.0
6	34.808857981994365	35.0	35.0	37.0	32.0	37.0
7	34.682923734452416	35.0	35.0	37.0	32.0	37.0
8	35.11210042058548	36.0	35.0	37.0	32.0	37.0
9	35.60198810971741	39.0	35.0	39.0	31.0	39.0
10	35.37602631831419	37.0	35.0	39.0	30.0	39.0
11	35.82893405256854	37.0	35.0	39.0	30.0	39.0
12	35.40205990944569	37.0	35.0	39.0	30.0	39.0
13	35.06288975337412	37.0	34.0	39.0	29.0	39.0
14	35.94924501966756	38.0	34.0	40.0	30.0	41.0
15	36.197142865985185	38.0	34.0	40.0	31.0	41.0
16	36.18022969723416	38.0	34.0	40.0	31.0	41.0
17	36.168512724335464	38.0	35.0	40.0	31.0	41.0
18	35.92097387665921	37.0	34.0	40.0	30.0	41.0
19	35.88669879085544	37.0	34.0	40.0	30.0	41.0
20	35.879781877377205	37.0	34.0	40.0	30.0	41.0
21	35.944085961605715	37.0	34.0	40.0	30.0	41.0
22	35.943033724208576	37.0	34.0	40.0	30.0	41.0
23	35.83859916254283	37.0	34.0	40.0	30.0	41.0
24	35.444003948985056	37.0	34.0	40.0	29.0	41.0
25	35.50838230879454	37.0	34.0	40.0	29.0	41.0
26	35.3833734111989	37.0	34.0	40.0	29.0	41.0
27	35.282055329118194	37.0	34.0	40.0	28.0	41.0
28	35.127599258482114	37.0	34.0	40.0	27.0	41.0
29	34.99586223117656	37.0	34.0	40.0	27.0	41.0
30	35.04099702588194	37.0	34.0	40.0	27.0	41.0
31	34.67731902290473	36.0	33.0	40.0	25.0	41.0
32	34.55607651622767	36.0	33.0	40.0	25.0	41.0
33	34.55395656735403	36.0	33.0	40.0	25.0	41.0
34	34.5157232120475	36.0	33.0	40.0	25.0	41.0
35	34.36262576558008	36.0	33.0	40.0	25.0	41.0
36	34.18403941557497	36.0	33.0	40.0	24.0	41.0
37	34.00253155938488	36.0	33.0	40.0	23.0	41.0
38	33.84809096282817	36.0	33.0	39.0	23.0	41.0
39	33.71850173773911	36.0	33.0	39.0	23.0	41.0
40	33.66790459301624	36.0	33.0	39.0	23.0	41.0
41	33.805818872806164	36.0	33.0	39.0	23.0	41.0
42	33.76597002361345	36.0	33.0	39.0	23.0	41.0
43	33.7169605194339	36.0	33.0	39.0	23.0	40.0
44	33.67967417778479	36.0	33.0	39.0	23.0	40.0
45	33.40832381677452	35.0	32.0	39.0	22.0	40.0
46	33.15782941993866	35.0	32.0	39.0	21.0	40.0
47	33.122520665633	35.0	32.0	39.0	20.0	40.0
48	33.03410796574658	35.0	32.0	39.0	20.0	40.0
49	32.95029106743294	35.0	31.0	39.0	20.0	40.0
50	32.829243534155935	35.0	31.0	39.0	20.0	40.0
51	30.792814456503912	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	7.0
11	12.0
12	9.0
13	11.0
14	10.0
15	32.0
16	82.0
17	170.0
18	393.0
19	751.0
20	1255.0
21	1839.0
22	2426.0
23	3177.0
24	3679.0
25	4013.0
26	4544.0
27	5617.0
28	6796.0
29	8590.0
30	11058.0
31	13854.0
32	18026.0
33	23811.0
34	37154.0
35	40217.0
36	32397.0
37	38236.0
38	40814.0
39	24135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.08833532948957	40.06177252484363	19.015786655772914	23.83410548989388
2	17.73484236555346	41.283296350283635	18.667310388368445	22.314550895794454
3	18.59055895469499	31.42568882864314	26.847837187926505	23.135915028735365
4	23.171195929698165	32.59831456327506	18.374231325107313	25.85625818191947
5	15.126222065418219	41.495600719235206	18.50359462863757	24.874582586709003
6	19.41161360604851	35.30689741613823	24.507227942473563	20.774261035339702
7	70.97960206857493	14.521495043652378	8.214260292583893	6.284642595188799
8	73.49135463185618	8.201571547500782	5.852296817600837	12.454777003042205
9	68.97508982703074	13.531772927169698	11.399135308444825	6.094001937354737
10	38.73873873873874	33.445675149556976	12.800158454572747	15.01542765713154
11	32.05362696946345	23.00438535409336	27.329390537909948	17.612597138533243
12	30.314959411489813	23.600446891412197	31.468706769290762	14.615886927807232
13	21.34834937995364	38.4239959643601	22.33807149643632	17.88958315924994
14	12.584140306572461	38.898431237833506	33.214492403774436	15.302936051819596
15	11.658480878680123	31.05214455265984	35.92121836711325	21.36815620154679
16	12.970992290813657	26.799558060293204	38.63196759108817	21.59748205780497
17	12.833582466011183	27.57728528941171	28.69080004085157	30.898332203725538
18	16.318654621643287	28.941480126639867	29.846713769764264	24.89315148195258
19	19.13586551168138	33.47940864258281	28.8696803983647	18.515045447371108
20	22.056133770321335	33.20520795615265	24.219719547785505	20.51893872574051
21	16.230761850823686	33.68459493502434	25.893705453994016	24.19093776015796
22	16.210026584468356	32.88953673701183	26.03173424197127	24.86870243654854
23	19.472890960352313	32.57912670485669	20.308491246313302	27.63949108847769
24	14.617743817331588	36.12826154907914	28.569792740180922	20.684201893408353
25	15.249705218788009	28.729485239275686	29.157188793052757	26.863620748883545
26	20.044503452267108	37.03751845283965	21.04165312684722	21.876324968046028
27	13.423454371582164	33.49766805623899	26.76860990155391	26.31026767062494
28	17.00446581930608	29.50814091315637	28.91765004441061	24.56974322312694
29	18.731373076958786	30.813534248779867	29.088483880651523	21.366608793609824
30	18.744061822041896	29.553634706503136	22.52097511458556	29.181328356869408
31	25.475286347838733	34.303558109810254	18.346996945416734	21.874158596934276
32	22.86233330547999	33.167760684078104	17.880917674802937	26.08898833563897
33	22.501787256167194	28.6852293722785	20.284970645671436	28.528012725882874
34	18.437365568935476	29.590153533815506	24.540342472324607	27.432138424924407
35	16.479894528675015	30.805487727507654	22.9366088864543	29.77800885736303
36	24.672800591728794	27.108111202923986	26.9533704092275	21.26571779611972
37	17.136923938710265	33.89813723032548	22.31857415643056	26.64636467453369
38	19.322173427291943	34.37473887491064	22.973437195354062	23.329650502443357
39	16.633706877609317	31.39969237530213	28.807474599298715	23.159126147789838
40	20.70617508611325	30.34095586483082	21.66433008068185	27.288538968374077
41	16.867365476091003	22.83602737055159	33.80684016204456	26.48976699131285
42	23.66667595111429	28.225649214999954	22.65064789970321	25.457026934182554
43	20.481800935253357	24.974235657849537	27.61844634053497	26.925517066362136
44	19.768136394725197	25.647977073604007	26.255489429656382	28.328397102014414
45	23.9650162013611	31.681011138242333	22.042207098888653	22.31176556150792
46	18.35411502192677	27.54107594368673	33.23553715171716	20.86927188266934
47	21.182776730698407	27.19383760263183	23.683078475246116	27.940307191423646
48	20.56505148226206	21.493496244440937	35.3301085351927	22.6113437381043
49	21.51082721333494	21.226723116108207	30.53623874647578	26.726210924081073
50	19.122248321836093	24.380649973229843	25.03829834643988	31.458803358494187
51	16.990229666286005	25.438458038938972	31.917764552597944	25.65354774217708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2954.0
1	2423.5
2	1893.0
3	1375.5
4	858.0
5	867.0
6	876.0
7	972.0
8	1068.0
9	1173.5
10	1279.0
11	1405.0
12	1531.0
13	1543.5
14	1556.0
15	1610.0
16	1664.0
17	1633.5
18	1603.0
19	1584.0
20	1565.0
21	1498.0
22	1431.0
23	1477.0
24	1523.0
25	1650.0
26	1947.0
27	2117.0
28	2358.0
29	2599.0
30	2866.5
31	3134.0
32	3876.5
33	4619.0
34	5355.5
35	6092.0
36	6571.0
37	7050.0
38	8066.5
39	9083.0
40	11025.5
41	12968.0
42	18205.5
43	23443.0
44	34465.5
45	45488.0
46	44901.0
47	44314.0
48	41062.5
49	37811.0
50	34016.0
51	30221.0
52	26293.5
53	22366.0
54	19165.0
55	15964.0
56	13444.0
57	10924.0
58	9098.5
59	7273.0
60	6237.0
61	5201.0
62	4398.0
63	3595.0
64	3123.5
65	2652.0
66	2151.0
67	1650.0
68	1359.0
69	1068.0
70	772.0
71	476.0
72	433.5
73	391.0
74	374.0
75	346.5
76	336.0
77	261.0
78	186.0
79	117.5
80	49.0
81	53.0
82	57.0
83	41.5
84	26.0
85	23.0
86	20.0
87	12.5
88	5.0
89	4.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.002020626669456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98770657353487	25.407498803397733
2	10.523685235543649	6.946057544239766
3	3.6119390861365024	3.576038646688514
4	1.7379517307540386	2.294236754660026
5	1.053647130373923	1.7386242164915644
6	0.653143492631042	1.2933033009590746
7	0.49879467085280316	1.1522862411169907
8	0.39624433455031605	1.0461490961624351
9	0.3175922309140337	0.9433066819945417
>10	3.433777785441409	25.764976163446146
>50	0.6959149464612354	15.318294678736425
>100	0.07466898567180637	4.060389636648465
>500	0.006969105329368595	1.5205925909054132
>1k	0.004977932378120426	3.0888269116468865
>5k	0.002986759426872255	5.849418732906023
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	6139	1.8999074650053696	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	5849	1.8101578046614115	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGT	5815	1.799635430690051	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	3435	1.063069252694811	TruSeq Adapter, Index 13 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2496	0.7724660421328233	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	1292	0.39985021091170186	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	1108	0.34290559883139754	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	1070	0.33114529851046515	No Hit
AACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	985	0.3048393635820637	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	827	0.2559412727739763	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	624	0.19311651053320583	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTAT	589	0.18228465497445231	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.18197517338705932	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	512	0.1584545727451945	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	503	0.1556692384586579	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	462	0.1429804933755466	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCCACTATCGTATGCC	381	0.11791248479671701	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	353	0.1092470003497142	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.416497225497569	0.0
2	0.0	0.0	0.0	1.7312399998762074	0.0
3	0.0	0.0	0.0	2.4464519483413336	0.0
4	0.0	0.0	0.0	6.560081207968532	0.0
5	0.0	0.0	0.0	7.076296495739986	0.0
6	0.0	0.0	0.0	9.350057718316048	0.0
7	0.0	0.0	0.0	11.27936593412375	0.0
8	0.0	0.0	0.0	12.430018476050767	0.0
9	0.0	0.0	0.0	15.899616552313221	0.0
10	0.0	0.0	0.0	18.082699669783146	0.0
11	0.0	0.0	0.0	22.39254025581748	0.0
12	0.0	0.0	0.0	23.57290303013422	0.0
13	0.0	0.0	0.0	24.180724867773993	0.0
14	0.0	0.0	0.0	24.856323173052818	0.0
15	0.0	0.0	0.0	25.236985525546157	0.0
16	0.0	0.0	0.0	25.920630352097202	0.0
17	0.0	0.0	0.0	26.755611674883404	0.0
18	0.0	0.0	0.0	27.99848972985352	0.0
19	0.0	0.0	0.0	28.46487848205471	0.0
20	0.0012379263495718323	0.0	0.0	28.958501613946478	0.0
21	0.0012379263495718323	0.0	0.0	29.485239275689292	0.0
22	0.0012379263495718323	0.0	0.0	30.030545832675685	0.0
23	0.0012379263495718323	0.0	0.0	30.58761268998301	0.0
24	0.0012379263495718323	0.0	0.0	30.939493254848802	0.0
25	0.0012379263495718323	0.0	0.0	31.251760176528297	0.0
26	0.0012379263495718323	0.0	0.0	31.548553018838145	0.0
27	0.0012379263495718323	0.0	0.0	31.893624988781294	0.0
28	0.0012379263495718323	0.0	0.0	32.21641428443215	0.0
29	0.0012379263495718323	0.0	0.0	32.56736640453576	0.0
30	0.0012379263495718323	0.0	0.0	33.08079635802068	0.0
31	0.0012379263495718323	0.0	0.0	33.43670018352258	0.0
32	0.0012379263495718323	0.0	0.0	33.71925687281235	0.0
33	0.0012379263495718323	0.0	0.0	34.0247151995692	0.0
34	0.0012379263495718323	0.0	0.0	34.310057223145506	0.0
35	0.0012379263495718323	0.0	0.0	34.67400756991963	0.0
36	0.0012379263495718323	0.0	0.0	34.97884693350169	0.0
37	0.0012379263495718323	0.0	0.0	35.27316392311239	0.0
38	0.0012379263495718323	0.0	0.0	35.57367054447096	0.0
39	0.0012379263495718323	0.0	0.0	35.87850990805302	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGG	30	2.160641E-6	45.000004	6
CAGAACC	20	7.0257497E-4	45.000004	32
TGATAGT	20	7.0257497E-4	45.000004	33
AGTCCGC	20	7.0257497E-4	45.000004	38
GGAGCGT	30	2.160641E-6	45.000004	8
GGAGCAT	20	7.0257497E-4	45.000004	8
ATCTACG	20	7.0257497E-4	45.000004	1
GTTTCAA	20	7.0257497E-4	45.000004	24
ACCATAC	20	7.0257497E-4	45.000004	43
GAGTATA	20	7.0257497E-4	45.000004	27
GATCCAA	20	7.0257497E-4	45.000004	9
TAAGTCC	20	7.0257497E-4	45.000004	36
TACGGGT	20	7.0257497E-4	45.000004	4
TAACGCC	20	7.0257497E-4	45.000004	12
GCGTTGG	30	2.160641E-6	45.000004	2
ACGGGTC	40	6.7884685E-9	45.000004	5
TATGAGT	20	7.0257497E-4	45.000004	20
TAGCACG	20	7.0257497E-4	45.000004	1
TACGCGG	40	6.7884685E-9	45.000004	2
TCGGACC	20	7.0257497E-4	45.000004	12
>>END_MODULE