##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	640239
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.065142860712953	31.0	31.0	34.0	28.0	34.0
2	31.279136385006225	31.0	31.0	34.0	28.0	34.0
3	31.27672322367116	31.0	31.0	34.0	28.0	34.0
4	35.34794506426506	37.0	35.0	37.0	33.0	37.0
5	34.98747342789177	35.0	35.0	37.0	32.0	37.0
6	35.03610058118921	36.0	35.0	37.0	32.0	37.0
7	35.115435017235754	37.0	35.0	37.0	32.0	37.0
8	35.47235485498384	37.0	35.0	37.0	33.0	37.0
9	35.900680839499	39.0	37.0	39.0	32.0	39.0
10	35.53860980040266	37.0	35.0	39.0	30.0	39.0
11	36.021221762498065	37.0	35.0	39.0	31.0	39.0
12	35.96886631398587	37.0	35.0	39.0	32.0	39.0
13	35.70796843053922	37.0	35.0	39.0	30.0	39.0
14	36.711607696500835	38.0	35.0	40.0	31.0	41.0
15	36.74333491086922	38.0	35.0	40.0	31.0	41.0
16	36.79992159178057	38.0	35.0	40.0	32.0	41.0
17	36.74801753720095	38.0	35.0	40.0	32.0	41.0
18	36.627456309284504	38.0	35.0	40.0	31.0	41.0
19	36.63813357199421	38.0	35.0	40.0	31.0	41.0
20	36.556827997044856	38.0	35.0	40.0	31.0	41.0
21	36.54973064746134	38.0	35.0	40.0	31.0	41.0
22	36.576397251651336	38.0	35.0	40.0	31.0	41.0
23	36.470672670674546	38.0	35.0	40.0	31.0	41.0
24	36.090950410706	38.0	34.0	40.0	30.0	41.0
25	36.2702553265265	38.0	35.0	40.0	30.0	41.0
26	36.09152831989304	38.0	35.0	40.0	30.0	41.0
27	36.05267407952343	38.0	34.0	40.0	30.0	41.0
28	36.00663502223389	38.0	34.0	40.0	30.0	41.0
29	35.68838668059897	38.0	34.0	40.0	29.0	41.0
30	35.748490797967634	38.0	34.0	40.0	29.0	41.0
31	35.43862838721165	38.0	34.0	40.0	28.0	41.0
32	35.46165260160659	38.0	34.0	40.0	28.0	41.0
33	35.39920248532189	38.0	34.0	40.0	28.0	41.0
34	35.11225183095688	38.0	34.0	40.0	26.0	41.0
35	35.04285743292739	38.0	34.0	40.0	26.0	41.0
36	34.73642342937559	37.0	33.0	40.0	25.0	41.0
37	34.526679880482135	37.0	33.0	40.0	24.0	41.0
38	34.62820134356076	37.0	33.0	40.0	24.0	41.0
39	34.451222121738915	37.0	33.0	40.0	23.0	41.0
40	34.17363359620392	37.0	33.0	40.0	23.0	41.0
41	34.10495299411626	37.0	33.0	40.0	23.0	41.0
42	34.05413290974152	37.0	33.0	40.0	23.0	41.0
43	34.0265806987703	37.0	33.0	40.0	23.0	41.0
44	33.9970448535625	37.0	33.0	40.0	23.0	41.0
45	33.82364398295012	37.0	33.0	40.0	22.0	41.0
46	33.640404598907594	37.0	32.0	40.0	21.0	41.0
47	33.42064447807772	36.0	32.0	40.0	20.0	41.0
48	33.321378422745255	36.0	32.0	40.0	20.0	41.0
49	33.21788113501364	36.0	32.0	39.0	20.0	41.0
50	33.09873500364708	36.0	31.0	39.0	19.0	41.0
51	31.323154322057857	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	2.0
11	6.0
12	8.0
13	17.0
14	13.0
15	43.0
16	118.0
17	279.0
18	523.0
19	1079.0
20	1870.0
21	2839.0
22	4009.0
23	5392.0
24	6278.0
25	7499.0
26	8904.0
27	10742.0
28	12802.0
29	15496.0
30	19002.0
31	23816.0
32	30148.0
33	40115.0
34	61532.0
35	67623.0
36	65483.0
37	84895.0
38	101303.0
39	68394.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.42627831169298	32.95659902005345	20.681495504022717	25.93562716423086
2	21.50243893296097	32.46162761093904	21.28580108365782	24.750132372442167
3	20.297888757167247	34.27423196650001	20.799107833168552	24.628771443164194
4	18.811412613102295	33.77519957390912	19.661095309720274	27.75229250326831
5	16.4563545800865	36.3189371469092	19.12129689069238	28.103411382311915
6	19.727632962065726	38.626981486601096	19.952392778321844	21.69299277301133
7	81.79992159178056	7.125151701161597	6.074606514129879	5.000320192927953
8	84.08484956399094	6.004320261652289	4.657791855853829	5.253038318502934
9	74.80534612855512	12.596389785689407	8.52150525038306	4.076758835372416
10	34.921021680966014	39.21519932400244	12.089391617817721	13.77438737721382
11	27.09863035522672	25.073917708855596	31.112756330057994	16.714695605859685
12	28.43797394410525	23.768936287855006	30.808026377649593	16.985063390390152
13	22.477855925677755	32.37650314960507	27.3966440657317	17.74899685898547
14	16.14459600243034	34.436515113887154	31.164299581874893	18.254589301807606
15	14.678893350764325	30.95328463276995	35.32352762015435	19.04429439631138
16	17.011772166331635	27.795869979804415	34.54147591758703	20.65088193627692
17	16.859641477635694	27.577826405451717	30.117815378319655	25.44471673859293
18	18.024206585353284	28.612127658577503	29.839950393524916	23.5237153625443
19	20.36145876774142	32.87616030888465	27.883337316220974	18.879043607152955
20	22.61889700564945	30.12296970350135	26.847630338045636	20.410502952803565
21	18.83999568910985	32.58673714034915	27.054115728657578	21.519151441883423
22	18.391257014958477	29.342323725983576	27.567049180071816	24.69937007898613
23	18.604614839146006	31.562744537586745	24.937718570721245	24.894922052546004
24	16.080401225167478	31.749237394160616	29.771069866096877	22.399291514575026
25	18.350022413504956	29.685945404762908	28.435162494006143	23.52886968772599
26	19.19658127667949	33.228528721305636	26.141019213137596	21.43387078887728
27	16.112108134618477	31.241614459600243	28.692410178074123	23.953867227707153
28	20.6294836771893	29.39011837766834	30.49939163343689	19.481006311705475
29	19.30544687218367	28.741454363136263	30.67541964797521	21.277679116704856
30	21.639419029456185	28.82361118269896	28.668512852231743	20.868456935613107
31	25.742574257425744	29.34435421772182	24.456648220430182	20.456423304422255
32	26.127586729330766	27.171884249475585	26.884803956022672	19.815725065170973
33	26.88824017281047	26.161480322192183	26.305332852262982	20.64494665273437
34	23.092157772331895	26.54180704393203	28.844384675097896	21.521650508638178
35	23.861245566108906	25.2069930135465	31.226151484055176	19.705609936289417
36	25.995448574672896	24.62783429313116	31.821085563359937	17.555631568836013
37	23.109495047943035	27.50863349467933	30.2155913650996	19.16628009227804
38	25.546085133832836	25.98201609086607	29.129903051829082	19.341995723472017
39	24.16004023497475	25.271500174153715	29.65127085354063	20.917188737330903
40	25.471425514534417	25.105156043290084	28.025159354553537	21.398259087621966
41	21.050576425366152	23.27240296201887	32.96331526195686	22.713705350658113
42	24.10068739954923	24.9481834127568	29.143179343963737	21.80794984373023
43	20.721324380426683	25.200589154987433	30.79334436046539	23.284742104120493
44	23.286928787530904	24.83088346695531	28.50888496327153	23.373302782242256
45	24.876960010246172	26.453871132498957	27.28590417016146	21.383264687093416
46	22.002564667257072	26.234421833096704	31.034972877316125	20.7280406223301
47	22.316197544979296	25.90204595471379	28.472179920311007	23.30957657999591
48	22.172345014908494	22.455364324884926	34.57661904382582	20.795671616380755
49	22.293549752514295	22.205301457736876	32.2018808601163	23.299267929632528
50	21.35358826938065	23.605403607090476	28.380339217073626	26.66066890645525
51	20.490004513939326	23.94918147754198	31.915112950007728	23.645701058510962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3830.0
1	3188.0
2	2546.0
3	1931.5
4	1317.0
5	1293.5
6	1270.0
7	1414.5
8	1559.0
9	1675.5
10	1792.0
11	1951.0
12	2110.0
13	2233.5
14	2357.0
15	2483.0
16	2609.0
17	2581.5
18	2554.0
19	2586.0
20	2618.0
21	2589.0
22	2560.0
23	3039.5
24	3519.0
25	3768.5
26	4806.0
27	5594.0
28	6987.0
29	8380.0
30	9581.0
31	10782.0
32	12259.0
33	13736.0
34	15424.0
35	17112.0
36	19838.0
37	22564.0
38	22989.0
39	23414.0
40	27434.5
41	31455.0
42	38717.5
43	45980.0
44	52892.5
45	59805.0
46	63264.5
47	66724.0
48	70524.5
49	74325.0
50	69877.5
51	65430.0
52	57614.0
53	49798.0
54	41905.0
55	34012.0
56	28732.5
57	23453.0
58	20334.5
59	17216.0
60	14615.5
61	12015.0
62	10561.0
63	9107.0
64	7554.0
65	6001.0
66	4525.0
67	3049.0
68	2768.5
69	2488.0
70	1839.0
71	1190.0
72	941.5
73	693.0
74	564.5
75	350.0
76	264.0
77	222.5
78	181.0
79	180.5
80	180.0
81	123.5
82	67.0
83	69.0
84	71.0
85	53.0
86	35.0
87	23.0
88	11.0
89	8.5
90	6.0
91	3.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	640239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.71317437060356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15779536289538	29.061339439505318
2	9.507313392104761	6.98087308720633
3	3.413455487960192	3.759563596073286
4	1.6629540939328415	2.4420929448346183
5	0.9241647969764298	1.696451166928455
6	0.5903935517672164	1.3005132847985867
7	0.4316405715303198	1.1092826897613726
8	0.34308833623313784	1.0076689530117962
9	0.2720174237475481	0.8987960798897481
>10	2.848170040161771	23.872619238149664
>50	0.7139473089908166	18.271954147415613
>100	0.12953832791939215	6.812435905153666
>500	0.0033977266339512697	0.9015679652917944
>1k	0.002123579146219544	1.884841501979801
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	4566	0.713171175139284	TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3215	0.5021562260343403	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	1721	0.26880586780874016	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	1438	0.22460362458394442	No Hit
AATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	1148	0.17930803965394174	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	848	0.1324505380022148	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGT	843	0.13166957964135267	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	829	0.12948289623093875	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	781	0.12198569596666245	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	648	0.10121220356773017	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0441413284726484	0.0
2	0.0	0.0	0.0	1.3810467653485652	0.0
3	0.0	0.0	0.0	1.7818345961430029	0.0
4	0.0	0.0	0.0	3.9319691552685794	0.0
5	0.0	0.0	0.0	4.3425970614098794	0.0
6	0.0	0.0	0.0	6.247042120208235	0.0
7	0.0	0.0	0.0	7.801774025012534	0.0
8	0.0	0.0	0.0	8.845290586796493	0.0
9	0.0	0.0	0.0	10.410487333636345	0.0
10	0.0	0.0	0.0	12.705723956210102	0.0
11	0.0	0.0	0.0	15.112637624387142	0.0
12	0.0	0.0	0.0	16.323279275395596	0.0
13	0.0	0.0	0.0	16.83668130182635	0.0
14	0.0	0.0	0.0	17.270737958793514	0.0
15	0.0	0.0	0.0	17.66043618086371	0.0
16	0.0	0.0	0.0	18.39859802355058	0.0
17	0.0	0.0	0.0	19.343089065177224	0.0
18	0.0	0.0	0.0	20.445333695698015	0.0
19	0.0	0.0	0.0	21.001844623648356	0.0
20	0.0	0.0	0.0	21.51977620857211	0.0
21	0.0	0.0	0.0	22.101590187414388	0.0
22	0.0	0.0	0.0	22.789926886678256	0.0
23	0.0	0.0	0.0	23.466080635512675	0.0
24	0.0	0.0	0.0	23.935280418718634	0.0
25	3.123833443448462E-4	0.0	0.0	24.300925123274276	0.0
26	3.123833443448462E-4	0.0	0.0	24.69249764541054	0.0
27	3.123833443448462E-4	0.0	0.0	25.090317834433705	0.0
28	3.123833443448462E-4	0.0	0.0	25.473299814600484	0.0
29	3.123833443448462E-4	0.0	0.0	25.882678187364405	0.0
30	3.123833443448462E-4	0.0	0.0	26.452465407449406	0.0
31	3.123833443448462E-4	0.0	0.0	26.884179189333985	0.0
32	3.123833443448462E-4	0.0	0.0	27.28824704524404	0.0
33	3.123833443448462E-4	0.0	0.0	27.660920375047443	0.0
34	3.123833443448462E-4	0.0	0.0	28.023909821176154	0.0
35	3.123833443448462E-4	0.0	0.0	28.471242770277975	0.0
36	3.123833443448462E-4	0.0	0.0	28.859535267298618	0.0
37	3.123833443448462E-4	0.0	0.0	29.246734422614054	0.0
38	3.123833443448462E-4	0.0	0.0	29.6259678026487	0.0
39	3.123833443448462E-4	0.0	0.0	30.013947916324998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAG	30	2.1639898E-6	45.000004	25
GCGAAGA	30	2.1639898E-6	45.000004	16
TAACGCA	30	2.1639898E-6	45.000004	28
CGCTGCG	30	2.1639898E-6	45.000004	1
TCGGCAT	30	2.1639898E-6	45.000004	32
CCATCCG	30	2.1639898E-6	45.000004	1
TCATCCG	30	2.1639898E-6	45.000004	1
AATTACG	30	2.1639898E-6	45.000004	1
CGCCAGT	30	2.1639898E-6	45.000004	26
TATTTCG	25	3.8888626E-5	45.0	20
CTTAACG	25	3.8888626E-5	45.0	1
CAGGTCG	20	7.0309424E-4	45.0	8
AACGTAG	25	3.8888626E-5	45.0	1
TCGTTTA	35	1.2107921E-7	45.0	38
TCGCACG	35	1.2107921E-7	45.0	1
TCACCGC	20	7.0309424E-4	45.0	24
ATCTACG	20	7.0309424E-4	45.0	10
ATCTACC	70	0.0	45.0	15
GAATGCG	35	1.2107921E-7	45.0	1
CGAAACG	25	3.8888626E-5	45.0	1
>>END_MODULE