FastQCFastQC Report
Sat 18 Jun 2016
SRR3548841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646928
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC198083.0618554151312045No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT179132.7689325550911383No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT170922.6420250785249673No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT71241.101204461702075No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT36990.5717792397299235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34250.5294252219721515No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCT30980.47887863873568615No Hit
AACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG25080.3876783815200455No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTAT22000.34006875571933814No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT16610.2567519105681003No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT12180.1882744293027972No Hit
AATGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTC9400.1453021047164445No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.12397051913041326No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTGCCATC7900.12211559864467143No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA7760.11995152474463927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC207.030998E-445.014
TCGTTAG253.888908E-545.023
ACCGGAT253.888908E-545.037
CGAACAG207.030998E-445.01
CATATAG253.888908E-545.01
CGTATAG207.030998E-445.01
GTCGAGT253.888908E-545.017
GTCGAAC207.030998E-445.026
CCGATTC253.888908E-545.018
CGTGATA207.030998E-445.030
TAGAACG253.888908E-545.01
TAGCGAG207.030998E-445.01
GATCCGA207.030998E-445.038
ACCGATA207.030998E-445.011
ATTCGTA253.888908E-545.031
GTGATAA207.030998E-445.017
TTCCGCA207.030998E-445.015
CGATTCG207.030998E-445.01
GCTTGTA207.030998E-445.027
TGTTGCG207.030998E-445.013